EnhiA.00450.a

dual specificity protein phosphatase, putative

CENTER ID: EnhiA.00450.a
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.00450.a.A1.GE26070 Full length( EnhiA.00450.a ) 1 205
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.00450.a.A1.PS00214 Full length( EnhiA.00450.a ) 1 205

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_059820
RefSeq: XP_652900.1
UniProt: C4M341

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDIQEVGES NWMISTIEKG TLFLGSVYAA EDEEVIQEYQ IKSVLSILGY EVYLPSVIKE KKWIVLEDSS SENISSYFTE CFKFIDNALK PVLVHCEMGV SRSATIVIGY LMYKGKTLKE AYEYVQQRRK NISPNNGFMY QLYKYSEELY PHNEETMLFI YNKYGRKDTV DKFNERDKNK VESFVNQFSE CEFKWGSYFK TKYNY
NT Sequence
ATGTCAGATA TTCAAGAAGT TGGTGAATCA AATTGGATGA TTAGTACTAT TGAAAAAGGG ACGTTGTTTC TAGGTAGTGT TTATGCAGCA GAAGATGAAG AAGTTATTCA AGAATATCAA ATTAAATCTG TACTTTCTAT ACTTGGGTAT GAGGTATATT TACCTAGTGT TATTAAAGAA AAAAAGTGGA TTGTTTTAGA AGATTCTTCA AGTGAAAATA TAAGCTCTTA TTTTACTGAA TGTTTTAAAT TTATTGATAA TGCACTAAAA CCTGTATTGG TTCATTGTGA AATGGGGGTT TCTCGTTCTG CTACCATTGT TATTGGTTAT TTAATGTATA AAGGAAAGAC TTTAAAAGAG GCATATGAAT ATGTTCAACA AAGAAGAAAA AATATAAGCC CCAATAATGG TTTTATGTAT CAATTATATA AATACTCTGA AGAACTTTAT CCTCATAATG AAGAAACAAT GCTTTTTATA TATAATAAGT ATGGTAGAAA AGATACTGTT GACAAATTTA ATGAAAGAGA TAAAAATAAA GTAGAAAGTT TTGTAAATCA ATTTAGTGAA TGTGAATTTA AGTGGGGAAG TTATTTTAAA ACAAAATATA ATTAT
Details for EnhiA.00450.a.A1.GE26070
HARVESTED ON: 1/9/2009
SEQUENCED ON: 7/28/2010
EXPECTED MW: 26kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSDIQEVGE SNWMISTIEK GTLFLGSVYA AEDEEVIQEY QIKSVLSILG YEVYLPSVIK EKKWIVLEDS LSENISSYFT ECFKFIDNAL KPVLVHCEMG VSRSATIVIG YLMYKGKTLK EAYEYVQQRR KNISPNNGFM YQLYKYSEEL YPHNEETMLF IYNKYGRKDT VDKFNERDKN KVESFVNQFS ECEFKWGSYF KTKY
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtcag atattcaaga agttggtgaa tcaaattgga tgattagtac tattgaaaaa gggacgttgt ttctaggtag tgtttatgca gcagaagatg aagaagttat tcaagaatat caaattaaat ctgtactttc tatacttggg tatgaggtat atttacctag tgttattaaa gaaaaaaagt ggattgtttt agaagattct ttaagtgaaa atataagctc ttattttact gaatgtttta aatttattga taatgcacta aaacctgtat tggttcattg tgaaatgggg gtttctcgtt ctgctaccat tgttattggt tatttaatgt ataaaggaaa gactttaaaa gaggcatatg aatatgttca acaaagaaga aaaaatataa gccccaataa tggttttatg tatcaattat ataaatactc tgaagaactt tatcctcata atgaagaaac aatgcttttt atatataata agtatggtag aaaagatact gttgacaaat ttaatgaaag agataaaaat aaagtagaaa gttttgtaaa tcaatttagt gaatgtgaat ttaagtgggg aagttatttt aaaacaaaat ata
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSDIQEVGE SNWMISTIEK GTLFLGSVYA AEDEEVIQEY QIKSVLSILG YEVYLPSVIK EKKWIVLEDS SSENISSYFT ECFKFIDNAL KPVLVHCEMG VSRSATIVIG YLMYKGKTLK EAYEYVQQRR KNISPNNGFM YQLYKYSEEL YPHNEETMLF IYNKYGRKDT VDKFNERDKN KVESFVNQFS ECEFKWGSYF KTKYNY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcagatatt caagaagttg gtgaatcaaa ttggatgatt agtactattg aaaaagggac gttgtttcta ggtagtgttt atgcagcaga agatgaagaa gttattcaag aatatcaaat taaatctgta ctttctatac ttgggtatga ggtatattta cctagtgtta ttaaagaaaa aaagtggatt gttttagaag attcttcaag tgaaaatata agctcttatt ttactgaatg ttttaaattt attgataatg cactaaaacc tgtattggtt cattgtgaaa tgggggtttc tcgttctgct accattgtta ttggttattt aatgtataaa ggaaagactt taaaagaggc atatgaatat gttcaacaaa gaagaaaaaa tataagcccc aataatggtt ttatgtatca attatataaa tactctgaag aactttatcc tcataatgaa gaaacaatgc tttttatata taataagtat ggtagaaaag atactgttga caaatttaat gaaagagata aaaataaagt agaaagtttt gtaaatcaat ttagtgaatg tgaatttaag tggggaagtt attttaaaac aaaatataat tataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for EnhiA.00450.a.A1.PS00214
PURIFICATION DATe: 5/14/2009
CONCENTRATION: 16mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 17
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSDIQEVGE SNWMISTIEK GTLFLGSVYA AEDEEVIQEY QIKSVLSILG YEVYLPSVIK EKKWIVLEDS LSENISSYFT ECFKFIDNAL KPVLVHCEMG VSRSATIVIG YLMYKGKTLK EAYEYVQQRR KNISPNNGFM YQLYKYSEEL YPHNEETMLF IYNKYGRKDT VDKFNERDKN KVESFVNQFS ECEFKWGSYF KTKY
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtcag atattcaaga agttggtgaa tcaaattgga tgattagtac tattgaaaaa gggacgttgt ttctaggtag tgtttatgca gcagaagatg aagaagttat tcaagaatat caaattaaat ctgtactttc tatacttggg tatgaggtat atttacctag tgttattaaa gaaaaaaagt ggattgtttt agaagattct ttaagtgaaa atataagctc ttattttact gaatgtttta aatttattga taatgcacta aaacctgtat tggttcattg tgaaatgggg gtttctcgtt ctgctaccat tgttattggt tatttaatgt ataaaggaaa gactttaaaa gaggcatatg aatatgttca acaaagaaga aaaaatataa gccccaataa tggttttatg tatcaattat ataaatactc tgaagaactt tatcctcata atgaagaaac aatgcttttt atatataata agtatggtag aaaagatact gttgacaaat ttaatgaaag agataaaaat aaagtagaaa gttttgtaaa tcaatttagt gaatgtgaat ttaagtgggg aagttatttt aaaacaaaat ata
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSDIQEVGE SNWMISTIEK GTLFLGSVYA AEDEEVIQEY QIKSVLSILG YEVYLPSVIK EKKWIVLEDS SSENISSYFT ECFKFIDNAL KPVLVHCEMG VSRSATIVIG YLMYKGKTLK EAYEYVQQRR KNISPNNGFM YQLYKYSEEL YPHNEETMLF IYNKYGRKDT VDKFNERDKN KVESFVNQFS ECEFKWGSYF KTKYNY
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCAGATATT CAAGAAGTTG GTGAATCAAA TTGGATGATT AGTACTATTG AAAAAGGGAC GTTGTTTCTA GGTAGTGTTT ATGCAGCAGA AGATGAAGAA GTTATTCAAG AATATCAAAT TAAATCTGTA CTTTCTATAC TTGGGTATGA GGTATATTTA CCTAGTGTTA TTAAAGAAAA AAAGTGGATT GTTTTAGAAG ATTCTTCAAG TGAAAATATA AGCTCTTATT TTACTGAATG TTTTAAATTT ATTGATAATG CACTAAAACC TGTATTGGTT CATTGTGAAA TGGGGGTTTC TCGTTCTGCT ACCATTGTTA TTGGTTATTT AATGTATAAA GGAAAGACTT TAAAAGAGGC ATATGAATAT GTTCAACAAA GAAGAAAAAA TATAAGCCCC AATAATGGTT TTATGTATCA ATTATATAAA TACTCTGAAG AACTTTATCC TCATAATGAA GAAACAATGC TTTTTATATA TAATAAGTAT GGTAGAAAAG ATACTGTTGA CAAATTTAAT GAAAGAGATA AAAATAAAGT AGAAAGTTTT GTAAATCAAT TTAGTGAATG TGAATTTAAG TGGGGAAGTT ATTTTAAAAC AAAATATAAT TATAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA