EnhiA.00425.b

elongation factor 1-alpha 1

CENTER ID:	EnhiA.00425.b
ORGANISM:	Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE:	Amoebic dysentery
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EnhiA.00425.b.B1.GE36375	Full length( EnhiA.00425.b )		1	442

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:EHI_052400
OrthoMCL:	OG5_126631
RefSeq:	XP_648717.1
UniProt:	C4MAC9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPKEKTHINI VVIGHVDSGK STTTGHLIYK CGGIDQRTIE KFEKESAEMG KGSFKYAWVL DNLKAERERG ITIDISLWKF ETSKYYFTII DAPGHRDFIK NMITGTSQAD VAILIVAAGT GEFEAGISKN GQTREHILLS YTLGVKQMIV GVNKMDAIQY KQERYEEIKK EISAFLKKTG YNPDKIPFVP ISGFQGDNMI EPSTNMPWYK GPTLIGALDS VTPPERPVDK PLRLPLQDVY KISGIGTVPC GRVETGVLRP GTIVQFAPSG VSSECKSVEM HHTALAQAIP GDNVGFNVRN LTVKDIKRGN VASDAKNQPA VGCEDFTAQV IVMNHPGQIR KGYTPVLDCH TSHIACKFEE LLSKIDRRTG KSMEGGEPEY IKNGDSALVK IVPTKPLCVE EFAKFPPLGR FAVRDMKQTV AVGVVKAVTP RAANASAAGK KK

NT Sequence

ATGCCAAAGG AAAAGACACA TATTAATATT GTCGTTATTG GACACGTCGA TTCAGGAAAA AGTACTACTA CAGGACATTT AATTTATAAA TGTGGAGGAA TTGATCAAAG AACCATTGAA AAGTTTGAAA AAGAATCAGC TGAAATGGGA AAAGGATCAT TCAAATATGC ATGGGTATTA GATAATCTTA AGGCAGAAAG AGAAAGAGGA ATTACTATTG ATATTTCATT ATGGAAATTT GAAACATCTA AATATTATTT CACTATTATT GATGCACCAG GTCATAGAGA TTTCATTAAG AATATGATTA CTGGAACATC ACAAGCTGAT GTTGCTATTC TTATTGTTGC AGCTGGTACA GGAGAATTTG AAGCTGGTAT TTCAAAGAAT GGACAAACCA GAGAACATAT TCTTCTTTCA TATACACTTG GAGTTAAACA AATGATTGTT GGAGTTAATA AGATGGATGC TATTCAATAT AAACAAGAAA GATATGAAGA AATTAAGAAA GAAATTAGTG CATTCCTTAA GAAGACAGGA TATAATCCAG ACAAGATTCC ATTTGTCCCA ATTTCAGGAT TCCAAGGAGA TAATATGATT GAACCATCAA CCAACATGCC ATGGTACAAA GGACCAACAT TAATTGGAGC ACTTGATTCA GTCACACCAC CAGAAAGACC AGTTGATAAA CCACTTAGAC TTCCACTTCA AGATGTTTAT AAGATTTCAG GTATTGGAAC TGTACCATGT GGAAGAGTTG AAACTGGAGT TCTTAGACCA GGAACTATTG TTCAATTTGC ACCATCAGGA GTATCATCAG AATGTAAATC AGTTGAAATG CATCATACAG CACTTGCTCA AGCTATTCCA GGTGATAATG TTGGATTCAA TGTTAGAAAC TTAACAGTTA AAGATATTAA GAGAGGAAAT GTAGCATCAG ATGCTAAGAA TCAACCAGCT GTTGGATGTG AAGATTTCAC TGCTCAAGTT ATTGTTATGA ATCATCCAGG ACAAATTAGA AAGGGATATA CACCAGTTCT TGATTGCCAT ACATCACACA TTGCATGTAA ATTCGAAGAA TTATTAAGCA AGATTGATAG AAGAACAGGT AAATCCATGG AAGGAGGAGA ACCAGAATAT ATTAAGAATG GAGATTCAGC ACTTGTTAAG ATTGTTCCAA CTAAACCACT TTGTGTTGAA GAATTTGCTA AATTCCCACC ATTGGGAAGA TTTGCTGTTA GAGATATGAA ACAAACCGTT GCTGTTGGAG TTGTTAAGGC TGTTACCCCA AGAGCTGCTA ATGCCTCAGC TGCTGGAAAG AAGAAA

Details for EnhiA.00425.b.B1.GE36375

HARVESTED ON:	10/23/2012
SEQUENCED ON:	10/26/2012
EXPECTED MW:	49kDa
OBSERVED MW:	49kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMP KEKTHINIVV IGHVDSGKST TTGHLIYKCG GIDQRTIEKF EKESAEMGKG SFKYAWVLDN LKAERERGIT IDISLWKFET SKYYFTIIDA PGHRDFIKNM ITGTSQADVA ILIVAAGTGE FEAGISKNGQ TREHILLSYT LGVKQMIVGV NKMDAIQYKQ ERYEEIKKEI SAFLKKTGYN PDKIPFVPIS GFQGDNMIEP STNMPWYKGP TLIGALDSVT PPERPVDKPL RLPLQDVYKI SGIGTVPCGR VETGVLRPGT IVQFAPSGVS SECKSVEMHH TALAQAIPGD NVGFNVRNLT VKDIKRGNVA SDAKNQPAVG CEDFTAQVIV MNHPGQIRKG YTPVLDCHTS HIACKFEELL SKIDRRTGKS MEGGEPEYIK NGDSALVKIV PTKPLCVEEF AKFPPLGRFA VRDMKQTVAV GVVKAVTPRA ANASAAGKKK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccaa aggaaaagac acatattaat attgtcgtta ttggacacgt cgattcagga aaaagtacta ctacaggaca tttaatttat aaatgtggag gaattgatca aagaaccatt gaaaagtttg aaaaagaatc agctgaaatg ggaaaaggat cattcaaata tgcatgggta ttagataatc ttaaggcaga aagagaaaga ggaattacta ttgatatttc attatggaaa tttgaaacat ctaaatatta tttcactatt attgatgcac caggtcatag agatttcatt aagaatatga ttactggaac atcacaagct gatgttgcta ttcttattgt tgcagctggt acaggagaat ttgaagctgg tatttcaaag aatggacaaa ccagagaaca tattcttctt tcatatacac ttggagttaa acaaatgatt gttggagtta ataagatgga tgctattcaa tataaacaag aaagatatga agaaattaag aaagaaatta gtgcattcct taagaagaca ggatataatc cagacaagat tccatttgtc ccaatttcag gattccaagg agataatatg attgaaccat caaccaacat gccatggtac aaaggaccaa cattaattgg agcacttgat tcagtcacac caccagaaag accagttgat aaaccactta gacttccact tcaagatgtt tataagattt caggtattgg aactgtacca tgtggaagag ttgaaactgg agttcttaga ccaggaacta ttgttcaatt tgcaccatca ggagtatcat cagaatgtaa atcagttgaa atgcatcata cagcacttgc tcaagctatt ccaggtgata atgttggatt caatgttaga aacttaacag ttaaagatat taagagagga aatgtagcat cagatgctaa gaatcaacca gctgttggat gtgaagattt cactgctcaa gttattgtta tgaatcatcc aggacaaatt agaaagggat atacaccagt tcttgattgc catacatcac acattgcatg taaattcgaa gaattattaa gcaagattga tagaagaaca ggtaaatcca tggaaggagg agaaccagaa tatattaaga atggagattc agcacttgtt aagattgttc caactaaacc actttgtgtt gaagaatttg ctaaattccc accattggga agatttgctg ttagagatat gaaacaaacc gttgctgttg gagttgttaa ggctgttacc ccaagagctg ctaatgcctc agctgctgga aagaagaaat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat