EnhiA.00425.a

elongation factor 1-alpha 1

CENTER ID: EnhiA.00425.a
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.00425.a.B1.GE36374 Full length( EnhiA.00425.a ) 1 442
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_011210
OrthoMCL: OG5_126631
RefSeq: XP_651869.1
UniProt: C4M7D4
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPKEKTHINI VVIGHVDSGK STTTGHLIYK CGGIDQRTIE KFEKESAEMG KGSFKYAWVL DNLKAERERG ITIDISLWKF ETSKYYFTII DAPGHRDFIK NMITGTSQAD VAILIVAAGT GEFEAGISKN GQTREHILLS YTLGVKQMIV GVNKMDAIQY KQERYEEIKK EISAFLKKTG YNPDKIPFVP ISGFQGDNMI EPSTNMPWYK GPTLIGALDS VTPPERPVDK PLRLPLQDVY KISGIGTVPV GRVETGILKP GTIVQFAPSG VSSECKSIEM HHTALAQAIP GDNVGFNVRN LTVKDIKRGN VASDAKNQPA VGCEDFTAQV IVMNHPGQIR KGYTPVLDCH TSHIACKFEE LLSKIDRRTG KSMEGGEPEY IKNGDSALVK IVPTKPLCVE EFAKFPPLGR FAVRDMKQTV AVGVVKAVTP RAANASAAGK KK
NT Sequence
ATGCCAAAGG AAAAGACTCA TATTAATATT GTCGTTATTG GTCACGTCGA TTCTGGTAAA AGTACTACCA CTGGTCATCT TATCTACAAA TGTGGAGGAA TTGATCAAAG AACCATTGAA AAATTCGAAA AAGAATCAGC TGAAATGGGT AAAGGATCAT TCAAATATGC TTGGGTCTTA GATAATCTTA AAGCTGAAAG AGAAAGAGGA ATTACTATTG ATATTTCATT ATGGAAATTC GAAACATCTA AATACTACTT CACTATTATT GATGCCCCAG GTCACAGAGA TTTCATTAAG AACATGATTA CTGGAACTTC ACAAGCTGAT GTTGCCATCC TTATTGTTGC TGCTGGTACT GGTGAATTTG AAGCTGGTAT TTCAAAGAAT GGACAAACCA GAGAACACAT TCTTCTTTCA TACACTCTTG GAGTTAAACA AATGATTGTT GGTGTTAACA AGATGGATGC CATCCAATAC AAACAAGAAA GATATGAAGA AATTAAGAAA GAAATTAGTG CATTCCTTAA GAAGACAGGA TATAATCCAG ACAAGATTCC ATTTGTCCCA ATTTCAGGAT TCCAAGGAGA TAATATGATT GAACCATCAA CTAACATGCC ATGGTACAAA GGACCAACAT TAATTGGAGC ACTTGATTCA GTCACACCAC CAGAAAGACC AGTTGATAAA CCACTTAGAC TTCCACTTCA AGATGTTTAC AAGATTTCAG GTATTGGAAC TGTACCAGTC GGAAGAGTTG AAACTGGTAT CCTTAAACCA GGAACTATTG TTCAATTTGC ACCATCAGGA GTTTCATCTG AATGTAAATC AATTGAAATG CATCACACAG CACTTGCTCA AGCTATTCCA GGGGATAATG TTGGATTCAA TGTTAGAAAT TTAACAGTTA AAGATATTAA GAGAGGAAAT GTAGCATCAG ATGCTAAGAA TCAACCAGCT GTTGGATGTG AAGATTTCAC TGCTCAAGTC ATTGTCATGA ACCATCCAGG ACAAATTAGA AAGGGATATA CACCAGTTCT TGATTGCCAT ACATCACACA TTGCATGTAA ATTCGAAGAA TTATTAAGCA AGATTGATAG AAGAACAGGT AAATCCATGG AAGGAGGAGA ACCAGAATAT ATTAAGAATG GAGATTCAGC ACTTGTTAAG ATTGTTCCAA CTAAACCACT TTGTGTTGAA GAATTTGCTA AATTCCCACC ATTGGGAAGA TTTGCTGTTA GAGATATGAA ACAAACCGTT GCTGTTGGAG TTGTTAAGGC TGTTACCCCA AGAGCTGCTA ATGCCTCAGC TGCTGGAAAG AAGAAA
Details for EnhiA.00425.a.B1.GE36374
HARVESTED ON: 10/23/2012
SEQUENCED ON: 10/26/2012
EXPECTED MW: 49kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 66
PERCENT COVERAGE: 4
Validated AA Sequence
MAHHHHHHMI NTDKIMASIY S
Validated NT Sequence
aaagttcatg tgttggaaac ttaattccca atgcaatagt gttgagaagt ggggtctaat aagagatgat taggttatga gggctctccc cttatgaata gattgatgcc attattttat cagtattaat catatggtgg tggtggtggt gagccatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naaaccgttg tggtctccct atagtgannc gtattaanna ngcaatcann anctggtgta tatccctttc taatttgtcc tgnanggttc atgacnntga cttgnnnnnn gnantcttca catccnacan ctggntgant cntannatct gangctncat ttcctctcnn aatatcttta actgtnaaat ttctaacatt gaatccaaca ttannnnctg naatannttg agcaanngct gtgtgatgca tttcanttga tttacattca gatgaaactc nnganggtgc aaattgaaca atagttcctg gtttaaggat accagtttca actcttccga ctggtacagt tccnatacct gaaatcttgt aaacatcttg aagtggaagt ctaagtggtt tatcaactnn tctttctggt ggtgtgactg aatcaagtgc tccaattaat gttggtcctt tgtaccatgg catgttagtt gatggttcaa tcatattatc tccttggaat cctgaaattg ggacaaatgg aatcttgtct ggattatatc ctgtcttctt aaggaatgca ctaatttctt tcttaatttc ttcatatctt tcttgtttgt attggatggc atccatcttg ttaacaccaa caatcatttg tttaactcca agagtgtatg aaagaagaat gtgttctctg gtttgtccat tctttgaaat accagcttca aattcaccag taccagcagc aacaataagg atggcaacat cagcttgtga agttccagta atcatgttct taatgaaatc tctgtgacct ggggcatcaa taatagtgaa gtagtattta gatgtttcga atttccataa tgaaatatca atagtaattc ctctttctct ttcagcttta agattatcta agacccaagc atatttgaat gatcctttac ccatttcagc tgattctttt tcgaattttt caatggttct ttgatcaatt cctccacatt tgtagataag atgaccagtg gtagtacttt taccagaatc gacgtgacca ataacgacaa tattaatatg agtcttttcc tt
Expected Protein Sequence
MAHHHHHHMP KEKTHINIVV IGHVDSGKST TTGHLIYKCG GIDQRTIEKF EKESAEMGKG SFKYAWVLDN LKAERERGIT IDISLWKFET SKYYFTIIDA PGHRDFIKNM ITGTSQADVA ILIVAAGTGE FEAGISKNGQ TREHILLSYT LGVKQMIVGV NKMDAIQYKQ ERYEEIKKEI SAFLKKTGYN PDKIPFVPIS GFQGDNMIEP STNMPWYKGP TLIGALDSVT PPERPVDKPL RLPLQDVYKI SGIGTVPVGR VETGILKPGT IVQFAPSGVS SECKSIEMHH TALAQAIPGD NVGFNVRNLT VKDIKRGNVA SDAKNQPAVG CEDFTAQVIV MNHPGQIRKG YTPVLDCHTS HIACKFEELL SKIDRRTGKS MEGGEPEYIK NGDSALVKIV PTKPLCVEEF AKFPPLGRFA VRDMKQTVAV GVVKAVTPRA ANASAAGKKK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccaa aggaaaagac tcatattaat attgtcgtta ttggtcacgt cgattctggt aaaagtacta ccactggtca tcttatctac aaatgtggag gaattgatca aagaaccatt gaaaaattcg aaaaagaatc agctgaaatg ggtaaaggat cattcaaata tgcttgggtc ttagataatc ttaaagctga aagagaaaga ggaattacta ttgatatttc attatggaaa ttcgaaacat ctaaatacta cttcactatt attgatgccc caggtcacag agatttcatt aagaacatga ttactggaac ttcacaagct gatgttgcca tccttattgt tgctgctggt actggtgaat ttgaagctgg tatttcaaag aatggacaaa ccagagaaca cattcttctt tcatacactc ttggagttaa acaaatgatt gttggtgtta acaagatgga tgccatccaa tacaaacaag aaagatatga agaaattaag aaagaaatta gtgcattcct taagaagaca ggatataatc cagacaagat tccatttgtc ccaatttcag gattccaagg agataatatg attgaaccat caactaacat gccatggtac aaaggaccaa cattaattgg agcacttgat tcagtcacac caccagaaag accagttgat aaaccactta gacttccact tcaagatgtt tacaagattt caggtattgg aactgtacca gtcggaagag ttgaaactgg tatccttaaa ccaggaacta ttgttcaatt tgcaccatca ggagtttcat ctgaatgtaa atcaattgaa atgcatcaca cagcacttgc tcaagctatt ccaggggata atgttggatt caatgttaga aatttaacag ttaaagatat taagagagga aatgtagcat cagatgctaa gaatcaacca gctgttggat gtgaagattt cactgctcaa gtcattgtca tgaaccatcc aggacaaatt agaaagggat atacaccagt tcttgattgc catacatcac acattgcatg taaattcgaa gaattattaa gcaagattga tagaagaaca ggtaaatcca tggaaggagg agaaccagaa tatattaaga atggagattc agcacttgtt aagattgttc caactaaacc actttgtgtt gaagaatttg ctaaattccc accattggga agatttgctg ttagagatat gaaacaaacc gttgctgttg gagttgttaa ggctgttacc ccaagagctg ctaatgcctc agctgctgga aagaagaaat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat