EnhiA.00351.a

Meiotic recombination protein DMC1, putative

CENTER ID: EnhiA.00351.a
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.00351.a.A1.GE26042 Full length( EnhiA.00351.a ) 1 347
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_050430
RefSeq: XP_656580.1
UniProt: C4LTR6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTEVKSKTNE QEENVQEIEE DEVEEVPSFH SIDILQQQGI NVGDINKLKS AGCNTIESVV MHTHKELCAI RGFSDSKVDK IMEAVSKIFP THSFISATTS LERRANVIKI TTGSSQFDQL LGGGIETMSV TEMFGEFRTG KTQLCHTLAV TTQLPSHLKG GNGKVAYIDT EGTFRPERIA QIAERFGVDQ TAVLDNILIA RAYTHEQQFD LLIEVAARMA EDHFRMLIID SVTSLFRVDF SGRGELSERQ QKLGKMMNKL IKISEEFNVA VVITNQVMSD PGGGAMFVVD PKKPIGGHVI AHASTTRLYL RKGKGEQRIV KIYDSPNLPE AEATFAIDTG GIIDAKD
NT Sequence
ATGACTGAGG TGAAAAGTAA AACTAATGAA CAAGAAGAAA ATGTCCAAGA GATTGAAGAA GATGAAGTTG AAGAAGTTCC ATCATTTCAT TCAATTGATA TTCTTCAACA ACAAGGAATT AATGTTGGAG ATATTAATAA ATTGAAAAGT GCAGGATGTA ATACTATTGA ATCTGTTGTT ATGCATACTC ATAAAGAGTT ATGTGCTATT CGTGGATTTT CTGATTCAAA AGTGGATAAA ATTATGGAAG CAGTTTCTAA AATATTTCCT ACACATTCAT TTATAAGTGC AACAACATCA TTAGAAAGAA GAGCTAATGT TATTAAAATT ACAACAGGTA GTAGTCAATT TGATCAATTA CTTGGTGGAG GGATTGAAAC AATGAGTGTT ACTGAAATGT TTGGAGAGTT TAGAACAGGA AAAACTCAGT TGTGTCATAC ATTAGCAGTG ACAACTCAAC TCCCATCACA TTTAAAAGGA GGAAATGGAA AAGTAGCATA TATTGATACT GAAGGAACAT TTAGACCAGA AAGAATAGCA CAAATTGCAG AGAGATTTGG AGTTGACCAA ACAGCAGTAT TAGATAATAT ATTAATTGCT AGAGCATATA CTCATGAACA ACAATTTGAT TTGTTGATTG AAGTTGCAGC AAGAATGGCA GAAGACCATT TTAGAATGCT TATTATTGAT AGTGTAACGT CTCTGTTCAG GGTTGATTTT TCAGGAAGAG GAGAGTTAAG TGAAAGACAA CAAAAATTAG GAAAAATGAT GAATAAATTA ATCAAAATTA GTGAAGAGTT TAATGTTGCA GTTGTAATTA CAAACCAAGT AATGAGTGAT CCAGGAGGAG GTGCTATGTT TGTTGTTGAT CCAAAAAAAC CAATTGGTGG GCATGTAATA GCACATGCCA GTACAACACG TCTTTATCTT AGGAAAGGAA AAGGAGAACA ACGAATTGTT AAAATATATG ACTCTCCAAA TTTACCTGAA GCAGAAGCTA CTTTTGCAAT TGATACAGGT GGAATTATTG ATGCTAAAGA T
Details for EnhiA.00351.a.A1.GE26042
HARVESTED ON: 1/9/2009
SEQUENCED ON: 7/28/2010
EXPECTED MW: 40kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTEVKSKTN EQEENVQEIE EDEVEEVPSF HSIDILQQQG INVGDINKLK SAGCNTIESV VMHTHKELCA IRGFSDSKVD KIMEAVSKIF PTHSFISATT SLERRANVIK ITTGSSQFDQ LLGGGIETMS VTEMFGEFRT GKTQLCHTLA VTTQLPSHLK GGNGKVAYID TEGTFRPERI AQIAERFGVD QTAVLDNILI ARAYTHEQQF DLLIEVAARM AEDXFRMLII DSVTSLFRVD FSGRGELSER QQKLGKXMNK LIKISEEFNV AVVITNQVMS DPGGGXMFVV DPKKPIGGHV IAHASTTXLY XRKGKGEQRI VKIYDSPNLP EAEXXFAIDT GGIID
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgactg aggtgaaaag taaaactaat gaacaagaag aaaatgtcca agagattgaa gaagatgaag ttgaagaagt tccatcattt cattcaattg atattcttca acaacaagga attaatgttg gagatattaa taaattgaaa agtgcaggat gtaatactat tgaatctgtt gttatgcata ctcataaaga gttatgtgct attcgtggat tttctgattc aaaagtggat aaaattatgg aagcagtttc taaaatattt cctacacatt catttataag tgcaacaaca tcattagaaa gaagagctaa tgttattaaa attacaacag gtagtagtca atttgatcaa ttacttggtg gagggattga aacaatgagt gttactgaaa tgtttggaga gtttagaaca ggaaaaactc agttgtgtca tacattagca gtgacaactc aactcccatc acatttaaaa ggaggaaatg gaaaagtagc atatattgat actgaaggaa catttagacc agaaagaata gcacaaattg cagagagatt tggagttgac caaacagcag tattagataa tatattaatt gctagagcat atactcatga acaacaattt gatttgttga ttgaagttgc agcaagaatg gcagaagacc nttttagaat gcttattatt gatagtgtaa cgtctctgtt cagggttgat ttttcaggaa gaggagagtt aagtgaaaga caacaaaaat taggaaaaan gatgaataaa ttaatcaaaa ttagtgaaga gtttaatgtt gcagttgtaa ttacaaacca agtaatgagt gatccaggag gaggtnnnat gtttgttgtt gatccaaaaa aaccaattgg tgggcatgta atagcacatg ccagtacaac acntctttat nttaggaaag gaaaaggaga acaacgaatt gttaaaatat atgactctcc aaatttaccn gaagcagaag nnanttttgc aattgataca ggtggaatta ttgatnnt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTEVKSKTN EQEENVQEIE EDEVEEVPSF HSIDILQQQG INVGDINKLK SAGCNTIESV VMHTHKELCA IRGFSDSKVD KIMEAVSKIF PTHSFISATT SLERRANVIK ITTGSSQFDQ LLGGGIETMS VTEMFGEFRT GKTQLCHTLA VTTQLPSHLK GGNGKVAYID TEGTFRPERI AQIAERFGVD QTAVLDNILI ARAYTHEQQF DLLIEVAARM AEDHFRMLII DSVTSLFRVD FSGRGELSER QQKLGKMMNK LIKISEEFNV AVVITNQVMS DPGGGAMFVV DPKKPIGGHV IAHASTTRLY LRKGKGEQRI VKIYDSPNLP EAEATFAIDT GGIIDAKD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gactgaggtg aaaagtaaaa ctaatgaaca agaagaaaat gtccaagaga ttgaagaaga tgaagttgaa gaagttccat catttcattc aattgatatt cttcaacaac aaggaattaa tgttggagat attaataaat tgaaaagtgc aggatgtaat actattgaat ctgttgttat gcatactcat aaagagttat gtgctattcg tggattttct gattcaaaag tggataaaat tatggaagca gtttctaaaa tatttcctac acattcattt ataagtgcaa caacatcatt agaaagaaga gctaatgtta ttaaaattac aacaggtagt agtcaatttg atcaattact tggtggaggg attgaaacaa tgagtgttac tgaaatgttt ggagagttta gaacaggaaa aactcagttg tgtcatacat tagcagtgac aactcaactc ccatcacatt taaaaggagg aaatggaaaa gtagcatata ttgatactga aggaacattt agaccagaaa gaatagcaca aattgcagag agatttggag ttgaccaaac agcagtatta gataatatat taattgctag agcatatact catgaacaac aatttgattt gttgattgaa gttgcagcaa gaatggcaga agaccatttt agaatgctta ttattgatag tgtaacgtct ctgttcaggg ttgatttttc aggaagagga gagttaagtg aaagacaaca aaaattagga aaaatgatga ataaattaat caaaattagt gaagagttta atgttgcagt tgtaattaca aaccaagtaa tgagtgatcc aggaggaggt gctatgtttg ttgttgatcc aaaaaaacca attggtgggc atgtaatagc acatgccagt acaacacgtc tttatcttag gaaaggaaaa ggagaacaac gaattgttaa aatatatgac tctccaaatt tacctgaagc agaagctact tttgcaattg atacaggtgg aattattgat gctaaagata aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a