SSGCID: Target Page

EnhiA.00335.c

aldose reductase, putative

CENTER ID:	EnhiA.00335.c
ORGANISM:	Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE:	Amoebic dysentery
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EnhiA.00335.c.A1.PW26090	Full length( EnhiA.00335.c )		1	305

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:EHI_029620
RefSeq:	XP_648383.1
UniProt:	C4M7W2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDFYFTLNNG YKIPKLGLGT WMSANGEVGK AVEIAIKNGY RHIDCAKAYG NEKEVGDGIK SAIAKGYVKR EELFVTTKLW STDKHKEDVR PACLESLKKL QLEYLDLYII HIPLTADKKT GEFTEEIIPI EETWREMEKL VEEGLVKSIG VSNFNIKKLE ELLAIAKIQP AVNQFEFHIY YQRPKLHQFC KKHNIHITGY CPLGNPGISS GVPAPFENEV VKAIAKKHNK KAAQICIKFS IASGHSVIPK SVHEERIKEN GEVFDFELDE EDMEKLRGLD KNIKVCDMSF YFKDENKRKE FWDDE

NT Sequence

ATGGACTTTT ACTTTACATT AAACAATGGT TATAAAATTC CTAAACTTGG ACTTGGAACA TGGATGTCAG CGAATGGAGA AGTTGGAAAA GCAGTAGAGA TTGCGATAAA GAATGGATAT CGACATATTG ATTGTGCAAA AGCATATGGA AATGAGAAAG AGGTTGGAGA TGGGATTAAG AGTGCTATAG CAAAGGGGTA TGTTAAAAGA GAGGAATTAT TTGTTACAAC CAAATTATGG AGTACTGATA AACATAAAGA AGATGTTCGA CCAGCATGTC TTGAATCATT AAAGAAATTA CAATTAGAAT ATCTTGATCT TTATATTATT CATATTCCAT TAACAGCAGA TAAGAAAACA GGAGAATTTA CTGAAGAAAT AATTCCAATT GAAGAAACAT GGAGAGAAAT GGAGAAATTA GTTGAAGAAG GATTAGTTAA AAGTATTGGA GTATCTAATT TCAATATTAA AAAGTTAGAA GAGTTATTAG CTATTGCTAA AATCCAACCA GCAGTTAATC AATTTGAATT TCATATTTAT TATCAACGAC CAAAATTACA CCAATTCTGT AAAAAACATA ATATTCATAT CACAGGATAT TGTCCATTAG GAAATCCAGG AATTAGTTCA GGAGTTCCAG CGCCATTTGA GAATGAAGTT GTTAAGGCTA TTGCAAAGAA ACACAATAAG AAAGCAGCAC AAATTTGTAT TAAATTCAGT ATTGCAAGTG GACATTCAGT CATTCCTAAA TCAGTTCATG AAGAAAGAAT TAAAGAGAAT GGAGAAGTAT TTGACTTTGA ACTTGATGAA GAAGATATGG AGAAATTAAG AGGATTAGAC AAGAACATAA AAGTCTGTGA TATGTCGTTT TATTTTAAGG ACGAAAACAA AAGAAAAGAG TTTTGGGATG ATGAA

Details for EnhiA.00335.c.A1.PW26090

PURIFICATION DATe:	3/10/2009
CONCENTRATION:	26.67mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	19
VIAL VOLUME:	100µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMDFYFTLNN GYKIPKLGLG TWMSANGEVG KAVEIAIKNG YRHIDCAKAY GNEKEVGDGI KSAIAKGYVK REELFVTTKL WSTDKHKEDV RPACLESLKK LQLEYLDLYI IHIPLTADKK TGEFTEEIIP IEETWREMEK LVEEGLVKSI GVSNFNIKKL EELLAIAKIQ PAVNQFEFHI YYQRPKLHQF CKKHNIHITG YCPLGNPGIS SGVPAPFENE VVKAIAKKHN KKAAQICIKF SIASGHSVIP KSVHEERIKE NGEVFDFELD EEDMEKLRGL DKNIKVCDMS FYFKDENKRK EFWDDE

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGACTTTTAC TTTACATTAA ACAATGGTTA TAAAATTCCT AAACTTGGAC TTGGAACATG GATGTCAGCG AATGGAGAAG TTGGAAAAGC AGTAGAGATT GCGATAAAGA ATGGATATCG ACATATTGAT TGTGCAAAAG CATATGGAAA TGAGAAAGAG GTTGGAGATG GGATTAAGAG TGCTATAGCA AAGGGGTATG TTAAAAGAGA GGAATTATTT GTTACAACCA AATTATGGAG TACTGATAAA CATAAAGAAG ATGTTCGACC AGCATGTCTT GAATCATTAA AGAAATTACA ATTAGAATAT CTTGATCTTT ATATTATTCA TATTCCATTA ACAGCAGATA AGAAAACAGG AGAATTTACT GAAGAAATAA TTCCAATTGA AGAAACATGG AGAGAAATGG AGAAATTAGT TGAAGAAGGA TTAGTTAAAA GTATTGGAGT ATCTAATTTC AATATTAAAA AGTTAGAAGA GTTATTAGCT ATTGCTAAAA TCCAACCAGC AGTTAATCAA TTTGAATTTC ATATTTATTA TCAACGACCA AAATTACACC AATTCTGTAA AAAACATAAT ATTCATATCA CAGGATATTG TCCATTAGGA AATCCAGGAA TTAGTTCAGG AGTTCCAGCG CCATTTGAGA ATGAAGTTGT TAAGGCTATT GCAAAGAAAC ACAATAAGAA AGCAGCACAA ATTTGTATTA AATTCAGTAT TGCAAGTGGA CATTCAGTCA TTCCTAAATC AGTTCATGAA GAAAGAATTA AAGAGAATGG AGAAGTATTT GACTTTGAAC TTGATGAAGA AGATATGGAG AAATTAAGAG GATTAGACAA GAACATAAAA GTCTGTGATA TGTCGTTTTA TTTTAAGGAC GAAAACAAAA GAAAAGAGTT TTGGGATGAT GAAAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA