EnhiA.00292.a
Hydrolase TatD family protein
| CENTER ID: | EnhiA.00292.a |
| ORGANISM: | Entamoeba histolytica HM-1:IMSS |
| ASSOCIATED DISEASE: | Amoebic dysentery |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| EnhiA.00292.a.A1.GE26026 | Full length( EnhiA.00292.a ) | 1 | 302 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| EnhiA.00292.a.A1.PS00165 | Full length( EnhiA.00292.a ) | 1 | 302 |
Structures
3IPW
DEPOSITED: 8/18/2009
DETERMINATION: XRay
CLONE: EnhiA.00292.a.A1.GE26026
PROTEIN: EnhiA.00292.a.A1.PS00165
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | AmoebaDB:EHI_119490 |
| OrthoMCL: | OG5_126665 |
| RefSeq: | XP_651470.1 |
| UniProt: | C4M7X7 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAQQFIDIGA NLTDDNYFGN YHGKHYHEED IDVVLQRAER NGLSHIIITS GCLNDFKKAI EIINKYQNLT NIKLVTTIGV HPTRTNELKQ EGYLDELLLL CEKNIDKVVA IGEIGLDYER LQFSDKETQL SGYRTLSILH QKYPYLPFFF HCRKSWSDLC QLNKELGYNG CKGVVHCFDG TEEEMNQILN EGWDIGVTGN SLQSIELLNV MKQIPIERLH IETDCPYCGI KKTSAGFKYL KEKDFGVKVE KYQRNKYVQR RNEPSNIIDI AIIMSSIKHI SLFEFVNKVY SNSMNMYFPT MN
NT Sequence
ATGGCACAAC AATTTATTGA TATTGGTGCA AATTTGACTG ATGACAATTA TTTTGGTAAT TATCATGGAA AACACTACCA TGAAGAGGAT ATCGATGTTG TACTTCAACG TGCTGAAAGA AATGGATTAA GTCATATTAT TATTACATCA GGTTGTCTTA ACGATTTTAA GAAAGCTATT GAAATCATTA ATAAGTACCA AAATTTAACT AACATTAAAT TGGTTACTAC AATTGGTGTT CATCCAACTC GTACAAATGA GTTAAAACAA GAAGGATATT TAGATGAACT ACTTTTATTA TGTGAAAAAA ATATTGATAA AGTTGTGGCT ATAGGAGAAA TTGGATTAGA TTATGAAAGA CTTCAATTTT CTGATAAAGA AACTCAACTT AGTGGATATC GTACATTAAG CATTCTTCAT CAAAAATATC CTTATTTGCC TTTCTTCTTT CATTGTAGAA AATCTTGGTC AGATTTATGT CAATTAAATA AAGAATTAGG ATATAATGGC TGTAAAGGAG TTGTTCATTG TTTTGATGGA ACAGAAGAAG AAATGAATCA AATACTAAAT GAGGGATGGG ATATTGGTGT TACAGGAAAT TCCTTACAAT CAATTGAGTT ATTAAATGTT ATGAAACAAA TACCAATTGA ACGTCTTCAT ATTGAAACTG ATTGTCCTTA TTGTGGAATT AAAAAAACAT CTGCAGGATT TAAGTATTTG AAAGAGAAAG ATTTTGGAGT CAAAGTAGAG AAATATCAAA GAAATAAATA TGTTCAAAGA AGAAATGAAC CATCTAACAT TATTGATATT GCAATAATCA TGTCAAGTAT CAAACATATT TCTTTATTTG AATTTGTTAA CAAAGTTTAT TCAAATTCAA TGAACATGTA TTTCCCAACT ATGAAT
Details for EnhiA.00292.a.A1.GE26026
| HARVESTED ON: | 1/9/2009 |
| SEQUENCED ON: | 7/28/2010 |
| EXPECTED MW: | 37kDa |
| OBSERVED MW: | 38kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAQQFIDIG ANLTDDNYFG NYHGKHYHEE DIDVVLQRAE RNGLSHIIIT SGCLNDFKKA IEIINKYQNL TNIKLVTTIG VHPTRTNELK QEGYLDELLL LCEKNIDKVV AIGEIGLDYE RLQFSDKETQ LSGYRTLSIL HQKYPYLPFF FHCRKSWSDL CQLNKELGYN GCKGVVHCFD GTEEEMNQIL NEGWDIGVTG NSLQSIELLN VMKQIPIERL HIETDCPYCG IKKTSAGFKY LKEKDFGVKV EKYQRNKYVQ RRNEPSNIID IAIIMSSIKH ISLFEFVNKV YSNSMNMYFP TMN
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcac aacaatttat tgatattggt gcaaatttga ctgatgacaa ttattttggt aattatcatg gaaaacacta ccatgaagag gatatcgatg ttgtacttca acgtgctgaa agaaatggat taagtcatat tattattaca tcaggttgtc ttaacgattt taagaaagct attgaaatca ttaataagta ccaaaattta actaacatta aattggttac tacaattggt gttcatccaa ctcgtacaaa tgagttaaaa caagaaggat atttagatga actactttta ttatgtgaaa aaaatattga taaagttgtg gctataggag aaattggatt agattatgaa agacttcaat tttctgataa agaaactcaa cttagtggat atcgtacatt aagcattctt catcaaaaat atccttattt gcctttcttc tttcattgta gaaaatcttg gtcagattta tgtcaattaa ataaagaatt aggatataat ggctgtaaag gagttgttca ttgttttgat ggaacagaag aagaaatgaa tcaaatacta aatgagggat gggatattgg tgttacagga aattccttac aatcaattga gttattaaat gttatgaaac aaataccaat tgaacgtctt catattgaaa ctgattgtcc ttattgtgga attaaaaaaa catctgcagg atttaagtat ttgaaagaga aagattttgg agtcaaagta gagaaatatc aaagaaataa atatgttcaa agaagaaatg aaccatctaa cattattgat attgcaataa tcatgtcaag tatcaaacat atttctttat ttgaatttgt taacaaagtt tattcaaatt caatgaacat gtatttccca actatgaatt aataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAQQFIDIG ANLTDDNYFG NYHGKHYHEE DIDVVLQRAE RNGLSHIIIT SGCLNDFKKA IEIINKYQNL TNIKLVTTIG VHPTRTNELK QEGYLDELLL LCEKNIDKVV AIGEIGLDYE RLQFSDKETQ LSGYRTLSIL HQKYPYLPFF FHCRKSWSDL CQLNKELGYN GCKGVVHCFD GTEEEMNQIL NEGWDIGVTG NSLQSIELLN VMKQIPIERL HIETDCPYCG IKKTSAGFKY LKEKDFGVKV EKYQRNKYVQ RRNEPSNIID IAIIMSSIKH ISLFEFVNKV YSNSMNMYFP TMN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcacaacaa tttattgata ttggtgcaaa tttgactgat gacaattatt ttggtaatta tcatggaaaa cactaccatg aagaggatat cgatgttgta cttcaacgtg ctgaaagaaa tggattaagt catattatta ttacatcagg ttgtcttaac gattttaaga aagctattga aatcattaat aagtaccaaa atttaactaa cattaaattg gttactacaa ttggtgttca tccaactcgt acaaatgagt taaaacaaga aggatattta gatgaactac ttttattatg tgaaaaaaat attgataaag ttgtggctat aggagaaatt ggattagatt atgaaagact tcaattttct gataaagaaa ctcaacttag tggatatcgt acattaagca ttcttcatca aaaatatcct tatttgcctt tcttctttca ttgtagaaaa tcttggtcag atttatgtca attaaataaa gaattaggat ataatggctg taaaggagtt gttcattgtt ttgatggaac agaagaagaa atgaatcaaa tactaaatga gggatgggat attggtgtta caggaaattc cttacaatca attgagttat taaatgttat gaaacaaata ccaattgaac gtcttcatat tgaaactgat tgtccttatt gtggaattaa aaaaacatct gcaggattta agtatttgaa agagaaagat tttggagtca aagtagagaa atatcaaaga aataaatatg ttcaaagaag aaatgaacca tctaacatta ttgatattgc aataatcatg tcaagtatca aacatatttc tttatttgaa tttgttaaca aagtttattc aaattcaatg aacatgtatt tcccaactat gaataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for EnhiA.00292.a.A1.PS00165
| PURIFICATION DATe: | 2/3/2009 |
| CONCENTRATION: | 44.6mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAQQFIDIG ANLTDDNYFG NYHGKHYHEE DIDVVLQRAE RNGLSHIIIT SGCLNDFKKA IEIINKYQNL TNIKLVTTIG VHPTRTNELK QEGYLDELLL LCEKNIDKVV AIGEIGLDYE RLQFSDKETQ LSGYRTLSIL HQKYPYLPFF FHCRKSWSDL CQLNKELGYN GCKGVVHCFD GTEEEMNQIL NEGWDIGVTG NSLQSIELLN VMKQIPIERL HIETDCPYCG IKKTSAGFKY LKEKDFGVKV EKYQRNKYVQ RRNEPSNIID IAIIMSSIKH ISLFEFVNKV YSNSMNMYFP TMN
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcac aacaatttat tgatattggt gcaaatttga ctgatgacaa ttattttggt aattatcatg gaaaacacta ccatgaagag gatatcgatg ttgtacttca acgtgctgaa agaaatggat taagtcatat tattattaca tcaggttgtc ttaacgattt taagaaagct attgaaatca ttaataagta ccaaaattta actaacatta aattggttac tacaattggt gttcatccaa ctcgtacaaa tgagttaaaa caagaaggat atttagatga actactttta ttatgtgaaa aaaatattga taaagttgtg gctataggag aaattggatt agattatgaa agacttcaat tttctgataa agaaactcaa cttagtggat atcgtacatt aagcattctt catcaaaaat atccttattt gcctttcttc tttcattgta gaaaatcttg gtcagattta tgtcaattaa ataaagaatt aggatataat ggctgtaaag gagttgttca ttgttttgat ggaacagaag aagaaatgaa tcaaatacta aatgagggat gggatattgg tgttacagga aattccttac aatcaattga gttattaaat gttatgaaac aaataccaat tgaacgtctt catattgaaa ctgattgtcc ttattgtgga attaaaaaaa catctgcagg atttaagtat ttgaaagaga aagattttgg agtcaaagta gagaaatatc aaagaaataa atatgttcaa agaagaaatg aaccatctaa cattattgat attgcaataa tcatgtcaag tatcaaacat atttctttat ttgaatttgt taacaaagtt tattcaaatt caatgaacat gtatttccca actatgaatt aataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAQQFIDIG ANLTDDNYFG NYHGKHYHEE DIDVVLQRAE RNGLSHIIIT SGCLNDFKKA IEIINKYQNL TNIKLVTTIG VHPTRTNELK QEGYLDELLL LCEKNIDKVV AIGEIGLDYE RLQFSDKETQ LSGYRTLSIL HQKYPYLPFF FHCRKSWSDL CQLNKELGYN GCKGVVHCFD GTEEEMNQIL NEGWDIGVTG NSLQSIELLN VMKQIPIERL HIETDCPYCG IKKTSAGFKY LKEKDFGVKV EKYQRNKYVQ RRNEPSNIID IAIIMSSIKH ISLFEFVNKV YSNSMNMYFP TMN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCACAACAA TTTATTGATA TTGGTGCAAA TTTGACTGAT GACAATTATT TTGGTAATTA TCATGGAAAA CACTACCATG AAGAGGATAT CGATGTTGTA CTTCAACGTG CTGAAAGAAA TGGATTAAGT CATATTATTA TTACATCAGG TTGTCTTAAC GATTTTAAGA AAGCTATTGA AATCATTAAT AAGTACCAAA ATTTAACTAA CATTAAATTG GTTACTACAA TTGGTGTTCA TCCAACTCGT ACAAATGAGT TAAAACAAGA AGGATATTTA GATGAACTAC TTTTATTATG TGAAAAAAAT ATTGATAAAG TTGTGGCTAT AGGAGAAATT GGATTAGATT ATGAAAGACT TCAATTTTCT GATAAAGAAA CTCAACTTAG TGGATATCGT ACATTAAGCA TTCTTCATCA AAAATATCCT TATTTGCCTT TCTTCTTTCA TTGTAGAAAA TCTTGGTCAG ATTTATGTCA ATTAAATAAA GAATTAGGAT ATAATGGCTG TAAAGGAGTT GTTCATTGTT TTGATGGAAC AGAAGAAGAA ATGAATCAAA TACTAAATGA GGGATGGGAT ATTGGTGTTA CAGGAAATTC CTTACAATCA ATTGAGTTAT TAAATGTTAT GAAACAAATA CCAATTGAAC GTCTTCATAT TGAAACTGAT TGTCCTTATT GTGGAATTAA AAAAACATCT GCAGGATTTA AGTATTTGAA AGAGAAAGAT TTTGGAGTCA AAGTAGAGAA ATATCAAAGA AATAAATATG TTCAAAGAAG AAATGAACCA TCTAACATTA TTGATATTGC AATAATCATG TCAAGTATCA AACATATTTC TTTATTTGAA TTTGTTAACA AAGTTTATTC AAATTCAATG AACATGTATT TCCCAACTAT GAATAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA