SSGCID: Target Page

EnhiA.00275.a

hypothetical protein, conserved

CENTER ID:	EnhiA.00275.a
ORGANISM:	Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE:	Amoebic dysentery
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EnhiA.00275.a.A1.GE26822	Full length( EnhiA.00275.a )		1	229

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:EHI_188790
RefSeq:	XP_655138.1
UniProt:	C4M2U6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSEESYPSVI GKINHLASQR EKPVCLIAVS KTKPKEAIQH LYNVYNHRVF GENYIQELHE KATELEEVCP DIEWHFIGRL QSNKLKLLVS TPHLKCVQTV HSIEIAEKLN KACINANKTI DVMVQINSSG EEQKGGVSVE EAISVVKEVM KCSNLHFIGI MTIGMVGDSK KNFTTMKQLA DTICSQEHLG SIEISMGMSS DYEQAIELGA TMVRVGTALF GARDYSKHQ

NT Sequence

ATGTCTGAAG AATCATATCC ATCAGTTATT GGTAAAATTA ATCATTTGGC TTCTCAAAGA GAAAAACCAG TTTGTTTGAT TGCTGTAAGT AAAACAAAAC CAAAGGAAGC TATACAACAT TTATATAATG TATACAACCA CCGTGTTTTT GGTGAGAATT ATATTCAAGA ACTTCATGAA AAAGCAACAG AATTAGAAGA AGTATGTCCA GATATTGAAT GGCATTTTAT TGGTAGATTA CAATCAAATA AATTGAAGCT TCTTGTTTCT ACACCTCATC TTAAGTGTGT TCAGACAGTT CATTCAATAG AAATTGCAGA GAAATTAAAT AAAGCATGTA TCAATGCAAA TAAAACTATT GATGTTATGG TCCAAATAAA TAGTAGTGGT GAAGAGCAAA AAGGTGGTGT TTCTGTTGAG GAAGCAATAA GCGTTGTTAA AGAAGTAATG AAATGTTCAA ATCTTCATTT CATTGGTATT ATGACAATTG GAATGGTTGG TGATTCTAAA AAGAATTTCA CAACTATGAA ACAATTAGCT GATACCATCT GTTCCCAAGA ACATTTAGGA TCAATTGAAA TTTCAATGGG AATGTCATCT GATTATGAAC AGGCTATTGA GTTAGGAGCT ACAATGGTTC GAGTAGGAAC TGCTTTATTT GGTGCACGTG ATTATTCGAA GCATCAA

Details for EnhiA.00275.a.A1.GE26822

HARVESTED ON:	5/1/2009
SEQUENCED ON:	3/16/2012
EXPECTED MW:	27kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMSEESYPSV IGKINHLASQ REKPVCLIAV SKTKPKEAIQ HLYNVYNHRV FGENYIQELH EKATELEEVC PDIEWHFIGR LQSNKLKLLV STPHLKCVQT VHSIEIAEKL NKACINANKT IDVMVQINSS GEEQKGGVSV EEAISVVKEV MKCSNLHFIG IMTIGMVGDS KKNFTTMKQL ADTICSQEHL GSIEISMGMS SDYEQAIELG ATMVRVGTAL FGARDYS

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtctg aagaatcata tccatcagtt attggtaaaa ttaatcattt ggcttctcaa agagaaaaac cagtttgttt gattgctgta agtaaaacaa aaccaaagga agctatacaa catttatata atgtatacaa ccaccgtgtt tttggtgaga attatattca agaacttcat gaaaaagcaa cagaattaga agaagtatgt ccagatattg aatggcattt tattggtaga ttacaatcaa ataaattgaa gcttcttgtt tctacacctc atcttaagtg tgttcagaca gttcattcaa tagaaattgc agagaaatta aataaagcat gtatcaatgc aaataaaact attgatgtta tggtccaaat aaatagtagt ggtgaagagc aaaaaggtgg tgtttctgtt gaggaagcaa taagcgttgt taaagaagta atgaaatgtt caaatcttca tttcattggt attatgacaa ttggaatggt tggtgattct aaaaagaatt tcacaactat gaaacaatta gctgatacca tctgttccca agaacattta ggatcaattg aaatttcaat gggaatgtca tctgattatg aacaggctat tgagttagga gctacaatgg ttcgagtagg aactgcttta tttggtgcac gtgattattc gaag

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctgaagaa tcatatccat cagttattgg taaaattaat catttggctt ctcaaagaga aaaaccagtt tgtttgattg ctgtaagtaa aacaaaacca aaggaagcta tacaacattt atataatgta tacaaccacc gtgtttttgg tgagaattat attcaagaac ttcatgaaaa agcaacagaa ttagaagaag tatgtccaga tattgaatgg cattttattg gtagattaca atcaaataaa ttgaagcttc ttgtttctac acctcatctt aagtgtgttc agacagttca ttcaatagaa attgcagaga aattaaataa agcatgtatc aatgcaaata aaactattga tgttatggtc caaataaata gtagtggtga agagcaaaaa ggtggtgttt ctgttgagga agcaataagc gttgttaaag aagtaatgaa atgttcaaat cttcatttca ttggtattat gacaattgga atggttggtg attctaaaaa gaatttcaca actatgaaac aattagctga taccatctgt tcccaagaac atttaggatc aattgaaatt tcaatgggaa tgtcatctga ttatgaacag gctattgagt taggagctac aatggttcga gtaggaactg ctttatttgg tgcacgtgat tattcgaagc atcaaaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa