EnhiA.00245.b

40S ribosomal protein S16, putative

CENTER ID:	EnhiA.00245.b
ORGANISM:	Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE:	Amoebic dysentery
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EnhiA.00245.b.A1.GE26021	Full length( EnhiA.00245.b )		1	158

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:EHI_038620
OrthoMCL:	OG5_126930
RefSeq:	XP_656860.1
UniProt:	C4LZP6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSSAVKATGK TQKKHTEALK SVQVFGKKKT AIAVCLCKEG KGMIRVNGVP LDLINPPVLR IKVFEPLFIV GKESYAKLDL KIRVTGGGQV AQAYAIRQAI AKALIAYNQK FVDETTKNEL KAKFLEYDRT LLVADPRRCE AKKFGGPGAR AKYQKSYR

NT Sequence

ATGTCTTCAG CAGTAAAAGC CACAGGAAAG ACTCAAAAGA AACATACTGA AGCTCTTAAG AGTGTTCAAG TTTTTGGAAA GAAAAAGACA GCTATTGCAG TATGCCTTTG CAAAGAAGGA AAAGGTATGA TTAGAGTTAA TGGTGTTCCA CTTGATCTCA TTAATCCACC AGTATTAAGA ATTAAAGTTT TTGAACCATT ATTCATTGTT GGTAAAGAAA GTTATGCTAA ATTAGACCTT AAAATTAGAG TCACTGGTGG TGGACAAGTT GCTCAAGCTT ATGCTATTAG ACAAGCTATT GCTAAAGCTC TTATTGCTTA TAACCAAAAG TTTGTTGATG AAACTACAAA GAATGAACTT AAAGCTAAAT TCCTTGAATA TGATAGAACT CTCCTTGTTG CTGATCCAAG AAGATGTGAA GCTAAGAAAT TCGGAGGACC AGGAGCTCGT GCTAAATACC AAAAATCATA CAGA

Details for EnhiA.00245.b.A1.GE26021

HARVESTED ON:	1/9/2009
SEQUENCED ON:	7/28/2010
EXPECTED MW:	19kDa
OBSERVED MW:	22kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMSSAVKATG KTQKKHTEAL KSVQVFGKKK TAIAVCLCKE GKGMIRVNGV PLDLINPPVL RIKVFEPLFI VGKESYAKLD LKIRVTGGGQ VAQAYAIRQA IAKALIAYNQ KFVDETTKNE LKAKFLEYDR TLLVADPRRC EAKKFGGPGA RAKY

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtctt cagcagtaaa agccacagga aagactcaaa agaaacatac tgaagctctt aagagtgttc aagtttttgg aaagaaaaag acagctattg cagtatgcct ttgcaaagaa ggaaaaggta tgattagagt taatggtgtt ccacttgatc tcattaatcc accagtatta agaattaaag tttttgaacc attattcatt gttggtaaag aaagttatgc taaattagac cttaaaatta gagtcactgg tggtggacaa gttgctcaag cttatgctat tagacaagct attgctaaag ctcttattgc ttataaccaa aagtttgttg atgaaactac aaagaatgaa cttaaagcta aattccttga atatgataga actctccttg ttgctgatcc aagaagatgt gaagctaaga aattcggagg accaggagct cgtgctaaat accaa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSSAVKATG KTQKKHTEAL KSVQVFGKKK TAIAVCLCKE GKGMIRVNGV PLDLINPPVL RIKVFEPLFI VGKESYAKLD LKIRVTGGGQ VAQAYAIRQA IAKALIAYNQ KFVDETTKNE LKAKFLEYDR TLLVADPRRC EAKKFGGPGA RAKYQKSYR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcttcagca gtaaaagcca caggaaagac tcaaaagaaa catactgaag ctcttaagag tgttcaagtt tttggaaaga aaaagacagc tattgcagta tgcctttgca aagaaggaaa aggtatgatt agagttaatg gtgttccact tgatctcatt aatccaccag tattaagaat taaagttttt gaaccattat tcattgttgg taaagaaagt tatgctaaat tagaccttaa aattagagtc actggtggtg gacaagttgc tcaagcttat gctattagac aagctattgc taaagctctt attgcttata accaaaagtt tgttgatgaa actacaaaga atgaacttaa agctaaattc cttgaatatg atagaactct ccttgttgct gatccaagaa gatgtgaagc taagaaattc ggaggaccag gagctcgtgc taaataccaa aaatcataca gaaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa