SSGCID: Target Page

EnhiA.00237.a

cysteine desulfurase, putative

CENTER ID:	EnhiA.00237.a
ORGANISM:	Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE:	Amoebic dysentery
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EnhiA.00237.a.A1.GE26014	Full length( EnhiA.00237.a )		1	390

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EnhiA.00237.a.A1.PW26068	Full length( EnhiA.00237.a )		1	390

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:EHI_136380
RefSeq:	XP_655257.1
UniProt:	Q769I7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQSTKSVYLD NNATTMVDPE VLNSMLPYFS EIYGNPNSLH AFGQKARKAL SDSLDIIYEC IGASDDDTVL ITANSTEGNN TVLKTMLARY ETMKGRNKII VSQIEHPSIS ESEKYLKERG IEVIKMPVNE DGVVDPKDLE RLIDDKTALV SCMWVNNETG LIMPVEELCK IAHDHGALFH SDATQAMGKI KVSVKDVPVD YLTFTAHKFH GPKGVGALFI RAGKPITPLL HGGEQMGGLR SGTIDTPSVV GMAVALKKAT HDINIENTYV RKLRDKLEAA LRTIPDVTIV GKPELRVPNT ILVAFKGVEG EAMLWDLNKH GIAASTGSAC ASESLQANPT FKAMKFGEDL SHTGIRLSLS RFNTEEEIDY TIDIIKKSVD RLRQLSSTYA

NT Sequence

ATGCAAAGTA CAAAATCAGT TTATCTTGAT AATAATGCTA CTACCATGGT TGATCCAGAA GTGTTAAATA GTATGCTTCC ATATTTTAGT GAAATTTATG GTAATCCAAA TTCACTTCAT GCATTTGGTC AAAAAGCTAG AAAAGCACTT TCAGATTCAT TAGATATTAT TTATGAATGT ATTGGTGCAA GTGATGATGA TACTGTTTTA ATTACTGCTA ATTCTACAGA AGGAAATAAT ACTGTATTAA AGACTATGTT AGCACGTTAT GAAACAATGA AAGGGAGAAA TAAAATTATT GTTTCTCAAA TTGAACATCC ATCAATTAGT GAATCTGAGA AATATTTAAA AGAAAGAGGT ATTGAAGTTA TTAAAATGCC TGTAAATGAA GATGGTGTTG TTGATCCTAA AGATTTAGAA AGACTAATTG ATGATAAAAC AGCTTTAGTT AGTTGTATGT GGGTAAATAA TGAAACGGGT TTAATTATGC CAGTTGAAGA ATTATGTAAA ATTGCACATG ATCATGGTGC ACTTTTCCAT TCTGATGCAA CTCAAGCTAT GGGAAAAATT AAAGTTAGTG TTAAAGATGT TCCTGTTGAT TATTTAACTT TTACTGCTCA TAAATTCCAT GGTCCAAAAG GTGTTGGTGC TTTATTCATT AGAGCAGGTA AACCAATTAC ACCATTATTA CATGGTGGAG AACAAATGGG TGGTTTAAGA TCTGGTACAA TTGATACACC AAGTGTTGTT GGAATGGCTG TAGCATTAAA GAAAGCAACT CATGACATTA ATATTGAAAA CACTTATGTT AGAAAATTAA GAGATAAACT CGAAGCTGCT CTTAGAACTA TTCCTGATGT TACTATCGTT GGTAAACCAG AATTAAGAGT CCCTAATACT ATTCTAGTTG CTTTTAAAGG TGTTGAAGGT GAGGCTATGT TATGGGATCT TAATAAACAT GGTATTGCCG CTTCTACTGG TAGTGCTTGT GCTTCTGAAT CACTTCAAGC TAATCCAACA TTTAAAGCAA TGAAATTTGG TGAAGACTTA TCTCATACTG GTATTCGTTT ATCCTTATCT CGTTTTAACA CTGAAGAAGA AATTGATTAT ACTATTGACA TTATTAAGAA ATCTGTTGAT CGTCTAAGAC AATTATCATC AACATATGCT

Details for EnhiA.00237.a.A1.GE26014

HARVESTED ON:	1/9/2009
SEQUENCED ON:	7/30/2010
EXPECTED MW:	45kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMQSTKSV YLDNNATTMV DPEVLNSMLP YFSEIYGNPN SLHAFGQKAR KALSDSLDII YECIGASDDD TVLITANSTE GNNTVLKTML ARYETMKGRN KIIVSQIEHP SISESEKYLK ERGIEVIKMP VNEDGVVDPK DLERLIDDKT ALVSCMWVNN ETGLIMPVEE LCKIAHDHGA LFHSDATQAM GKIKVSVKDV PVDYLTFTAH KFHGPKGVGA LFIRAGKPIT PLLHGGEQMG GLRSGTIDTP SVVGMAVALK KATHDINIEN TYVRKLRDKL EAALRTIPDV TIVGKPELRV PNTILVAFKG VEGEAMLWDL NKHGIAASTG SACASESLQA NPTFKAMKFG EDLSHTGIRL SLSRFNTEEE IDYTIDIIKK SVDRLRQLSS T

Validated NT Sequence

atgttgatga taattgtctt agacgatcaa cagatttctt aataatgtca atagtataat caatttcttc ttcagtgtta aaacgagata aggataaacg aataccagta tgagataagt cttcaccaaa tttcattgct ttaaatgttg gattagcttg aagtgattca gaagcacaag cactaccagt agaagcggca ataccatgtt tattaagatc ccataacata gcctcacctt caacaccttt aaaagcaact agaatagtat tagggactct taattctggt ttaccaacga tagtaacatc aggaatagtt ctaagagcag cttcgagttt atctcttaat tttctaacat aagtgttttc aatattaatg tcatgagttg ctttctttaa tgctacagcc attccaacaa cacttggtgt atcaattgta ccagatctta aaccacccat ttgttctcca ccatgtaata atggtgtaat tggtttacct gctctaatga ataaagcacc aacacctttt ggaccatgga atttatgagc agtaaaagtt aaataatcaa caggaacatc tttaacacta actttaattt ttcccatagc ttgagttgca tcagaatgga aaagtgcacc atgatcatgt gcaattttac ataattcttc aactggcata attaaacccg tttcattatt tacccacata caactaacta aagctgtttt atcatcaatt agtctttcta aatctttagg atcaacaaca ccatcttcat ttacaggcat tttaataact tcaatacctc tttcttttaa atatttctca gattcactaa ttgatggatg ttcaatttga gaaacaataa ttttatttct ccctttcatt gtttcataac gtgctaacat agtctttaat acagtattat ttccttctgt agaattagca gtaattaaaa cagtatcatc atcacttgca ccaatacatt cataaataat atctaatgaa tctgaaagtg cttttctagc tttttgacca aatgcatgaa gtgaatttgg attaccataa atttcactaa aatatggaag catactattt aacacttctg gatcaaccat ggtagtagca ttattatcaa gataaactga ttttgtactt tgcatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagcca

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMQSTKSVYL DNNATTMVDP EVLNSMLPYF SEIYGNPNSL HAFGQKARKA LSDSLDIIYE CIGASDDDTV LITANSTEGN NTVLKTMLAR YETMKGRNKI IVSQIEHPSI SESEKYLKER GIEVIKMPVN EDGVVDPKDL ERLIDDKTAL VSCMWVNNET GLIMPVEELC KIAHDHGALF HSDATQAMGK IKVSVKDVPV DYLTFTAHKF HGPKGVGALF IRAGKPITPL LHGGEQMGGL RSGTIDTPSV VGMAVALKKA THDINIENTY VRKLRDKLEA ALRTIPDVTI VGKPELRVPN TILVAFKGVE GEAMLWDLNK HGIAASTGSA CASESLQANP TFKAMKFGED LSHTGIRLSL SRFNTEEEID YTIDIIKKSV DRLRQLSSTY A

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaaagtaca aaatcagttt atcttgataa taatgctact accatggttg atccagaagt gttaaatagt atgcttccat attttagtga aatttatggt aatccaaatt cacttcatgc atttggtcaa aaagctagaa aagcactttc agattcatta gatattattt atgaatgtat tggtgcaagt gatgatgata ctgttttaat tactgctaat tctacagaag gaaataatac tgtattaaag actatgttag cacgttatga aacaatgaaa gggagaaata aaattattgt ttctcaaatt gaacatccat caattagtga atctgagaaa tatttaaaag aaagaggtat tgaagttatt aaaatgcctg taaatgaaga tggtgttgtt gatcctaaag atttagaaag actaattgat gataaaacag ctttagttag ttgtatgtgg gtaaataatg aaacgggttt aattatgcca gttgaagaat tatgtaaaat tgcacatgat catggtgcac ttttccattc tgatgcaact caagctatgg gaaaaattaa agttagtgtt aaagatgttc ctgttgatta tttaactttt actgctcata aattccatgg tccaaaaggt gttggtgctt tattcattag agcaggtaaa ccaattacac cattattaca tggtggagaa caaatgggtg gtttaagatc tggtacaatt gatacaccaa gtgttgttgg aatggctgta gcattaaaga aagcaactca tgacattaat attgaaaaca cttatgttag aaaattaaga gataaactcg aagctgctct tagaactatt cctgatgtta ctatcgttgg taaaccagaa ttaagagtcc ctaatactat tctagttgct tttaaaggtg ttgaaggtga ggctatgtta tgggatctta ataaacatgg tattgccgct tctactggta gtgcttgtgc ttctgaatca cttcaagcta atccaacatt taaagcaatg aaatttggtg aagacttatc tcatactggt attcgtttat ccttatctcg ttttaacact gaagaagaaa ttgattatac tattgacatt attaagaaat ctgttgatcg tctaagacaa ttatcatcaa catatgctaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa

Details for EnhiA.00237.a.A1.PW26068

PURIFICATION DATe:	3/10/2009
CONCENTRATION:	28.98mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	36
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCAAAGTACA AAATCAGTTT ATCTTGATAA TAATGCTACT ACCATGGTTG ATCCAGAAGT GTTAAATAGT ATGCTTCCAT ATTTTAGTGA AATTTATGGT AATCCAAATT CACTTCATGC ATTTGGTCAA AAAGCTAGAA AAGCACTTTC AGATTCATTA GATATTATTT ATGAATGTAT TGGTGCAAGT GATGATGATA CTGTTTTAAT TACTGCTAAT TCTACAGAAG GAAATAATAC TGTATTAAAG ACTATGTTAG CACGTTATGA AACAATGAAA GGGAGAAATA AAATTATTGT TTCTCAAATT GAACATCCAT CAATTAGTGA ATCTGAGAAA TATTTAAAAG AAAGAGGTAT TGAAGTTATT AAAATGCCTG TAAATGAAGA TGGTGTTGTT GATCCTAAAG ATTTAGAAAG ACTAATTGAT GATAAAACAG CTTTAGTTAG TTGTATGTGG GTAAATAATG AAACGGGTTT AATTATGCCA GTTGAAGAAT TATGTAAAAT TGCACATGAT CATGGTGCAC TTTTCCATTC TGATGCAACT CAAGCTATGG GAAAAATTAA AGTTAGTGTT AAAGATGTTC CTGTTGATTA TTTAACTTTT ACTGCTCATA AATTCCATGG TCCAAAAGGT GTTGGTGCTT TATTCATTAG AGCAGGTAAA CCAATTACAC CATTATTACA TGGTGGAGAA CAAATGGGTG GTTTAAGATC TGGTACAATT GATACACCAA GTGTTGTTGG AATGGCTGTA GCATTAAAGA AAGCAACTCA TGACATTAAT ATTGAAAACA CTTATGTTAG AAAATTAAGA GATAAACTCG AAGCTGCTCT TAGAACTATT CCTGATGTTA CTATCGTTGG TAAACCAGAA TTAAGAGTCC CTAATACTAT TCTAGTTGCT TTTAAAGGTG TTGAAGGTGA GGCTATGTTA TGGGATCTTA ATAAACATGG TATTGCCGCT TCTACTGGTA GTGCTTGTGC TTCTGAATCA CTTCAAGCTA ATCCAACATT TAAAGCAATG AAATTTGGTG AAGACTTATC TCATACTGGT ATTCGTTTAT CCTTATCTCG TTTTAACACT GAAGAAGAAA TTGATTATAC TATTGACATT ATTAAGAAAT CTGTTGATCG TCTAAGACAA TTATCATCAA CATATGCTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA