EnhiA.00235.b

Cysteine protease, putative

CENTER ID: EnhiA.00235.b
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.00235.b.A2.GE33553 mature protein 19 426
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_117650
RefSeq: XP_651581.1
UniProt: C4M680

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIGVVLVLLV LGSEGSSFNL NEDPVKLFEQ FKQEYKKEYV NLVEELRRKA VFIKNVKMMR EHNAKESSYT MGINKFADME GNELLATSSK IQNNRRMGAR RIQEKIKTEK VNLTKQTLKQ AVPANYTLCT SEAEYNYCGT NNIDQNVCGG CYAVGPAQHL STIYAYLTKQ ADNNKPKRKM LSAQQLIDCN TESWGGCGGG FAENVLNSVD GIYYADDYPF IDGNTECEDP TDCTRFKHEC KKLNLGYPLK FATSNKFTHF ANKNWEEIKE IIYTYHGFIS AMKVTESLYR YSGGIYKSQS CTGQITDHVI TVDGYGECDG HKFLWVRNSW GNDWGVEGGH FKIDWDTLCG INGIDHYEDG SQLQNNLYVE MELGDGDTAD YNPQDGAGEN KQDPIGCVVV SPTGDNDNKT TGIVITLFVL LMTFFI
NT Sequence
atgataggag ttgttttagt tttgctagta ttaggaagtg aaggaagttc atttaattta aatgaagatc cagttaagtt atttgagcaa tttaaacaag aatataaaaa agaatatgta aatcttgttg aagaattaag aagaaaagct gttttcataa aaaatgtaaa aatgatgaga gagcacaatg caaaagaaag ttcatataca atgggaatta ataaatttgc agatatggaa ggtaatgaac ttcttgcaac atcaagtaaa atacaaaata atagaagaat gggtgctaga agaattcaag aaaaaattaa aacagaaaaa gtaaatttaa caaaacaaac tcttaaacaa gctgttccag ctaattatac attatgtact tctgaagctg aatataatta ttgtggaacc aataatattg accaaaatgt atgtggtgga tgttatgctg ttggaccagc acaacattta tcaacaatct atgcttactt aactaaacaa gctgataata ataaaccaaa acgaaaaatg ttatctgccc aacaattaat tgattgtaat actgaatcat ggggtggatg tggaggagga tttgctgaaa atgttcttaa ttctgtagat ggaatttatt atgcagatga ttatccattt attgatggta atacagaatg tgaagaccct actgattgta caagatttaa acatgaatgt aagaaattaa atcttggtta tccattaaaa tttgctacta gtaataaatt tactcacttt gccaataaaa attgggaaga aataaaagaa attatttata catatcatgg atttattagt gcaatgaaag ttactgaatc attatataga tattcaggtg gtatttataa atctcaaagt tgtactggac aaattactga ccatgttatt actgttgatg gttatggaga atgtgatgga cataaattcc tttgggtaag aaattcatgg ggaaatgatt ggggagttga aggtggacat ttcaaaattg attgggatac tctttgtgga attaatggta ttgatcatta tgaagatggt tctcaacttc aaaataattt atatgttgaa atggaattag gagatggtga tactgctgat tacaatcctc aagatggtgc aggtgaaaac aagcaagacc caattggttg tgttgttgtt agtccaactg gtgataatga taacaaaaca acaggaattg ttattacttt attcgtctta ttaatgactt tcttcatcta a
Details for EnhiA.00235.b.A2.GE33553
HARVESTED ON: 10/31/2011
SEQUENCED ON: 11/7/2011
EXPECTED MW: 48kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 96
PERCENT COVERAGE: 67
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNLNEDPVK LFEQFKQEYK KEYVNLVEEL RRKAAFIKNV KMMREHSAKE SSYTMGINKF ADMEGNELLA TSSKIQNNRR MGARRIQEKI KTEKVNLTKQ TLKQAVPANY TLCTSEAEYN YCGTNNIDQN VCGGCYAVGP AQHLSTIYAY LTKQADNNKP KRKMLSAQQL IDCNTESWGG CGGGFAEXVL NSVDGIYYAD XYPFIDGNTE CEDPTDCTRX KHECKKLNLG YXLKFATSNX FTHFAXKNWE EIKEXIYTYX GFISAMKVT
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaatt taaatgaaga tccagttaag ttatttgagc aatttaaaca agaatataaa aaagaatatg taaatcttgt tgaagaatta agaagaaaag ctgctttcat aaaaaatgta aaaatgatga gagagcacag tgcaaaagaa agttcatata caatgggaat taataaattt gcagatatgg aaggtaatga acttcttgca acatcaagta aaatacaaaa taatagaaga atgggtgcta gaagaattca agaaaaaatt aaaacagaaa aagtaaattt aacaaaacaa actcttaaac aagctgttcc agctaattat acattatgta cttctgaagc tgaatataat tattgtggaa ccaataatat tgaccaaaat gtatgtggtg gatgttatgc tgttggacca gcacaacatt tatcaacaat ctatgcttac ttaactaaac aagctgataa taataaacca aaacgaaaaa tgttatctgc ccaacaatta attgattgta atactgaatc atggggtgga tgtggaggag gatttgctga anntgttctt aattctgtag atggaattta ttatgcagat gantatccat ttattgatgg taatacagaa tgtgaagacc ctactgattg tacaagantt aaacatgaat gtaagaaatt aaatcttggt tatcnattaa aatttgctac tagtaataan tttactcact ttgccnataa aaattgggaa gaaataaaag aaantattta tacatatcan ggatttatta gtgcaatgaa agttactg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNLNEDPVK LFEQFKQEYK KEYVNLVEEL RRKAVFIKNV KMMREHNAKE SSYTMGINKF ADMEGNELLA TSSKIQNNRR MGARRIQEKI KTEKVNLTKQ TLKQAVPANY TLCTSEAEYN YCGTNNIDQN VCGGCYAVGP AQHLSTIYAY LTKQADNNKP KRKMLSAQQL IDCNTESWGG CGGGFAENVL NSVDGIYYAD DYPFIDGNTE CEDPTDCTRF KHECKKLNLG YPLKFATSNK FTHFANKNWE EIKEIIYTYH GFISAMKVTE SLYRYSGGIY KSQSCTGQIT DHVITVDGYG ECDGHKFLWV RNSWGNDWGV EGGHFKIDWD TLCGINGIDH YEDGSQLQNN LYVEMELGDG DTADYNPQDG AGENKQDPIG CVVVSPTGDN DNKTTGIVIT LFVLLMTFFI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaatttaaat gaagatccag ttaagttatt tgagcaattt aaacaagaat ataaaaaaga atatgtaaat cttgttgaag aattaagaag aaaagctgtt ttcataaaaa atgtaaaaat gatgagagag cacaatgcaa aagaaagttc atatacaatg ggaattaata aatttgcaga tatggaaggt aatgaacttc ttgcaacatc aagtaaaata caaaataata gaagaatggg tgctagaaga attcaagaaa aaattaaaac agaaaaagta aatttaacaa aacaaactct taaacaagct gttccagcta attatacatt atgtacttct gaagctgaat ataattattg tggaaccaat aatattgacc aaaatgtatg tggtggatgt tatgctgttg gaccagcaca acatttatca acaatctatg cttacttaac taaacaagct gataataata aaccaaaacg aaaaatgtta tctgcccaac aattaattga ttgtaatact gaatcatggg gtggatgtgg aggaggattt gctgaaaatg ttcttaattc tgtagatgga atttattatg cagatgatta tccatttatt gatggtaata cagaatgtga agaccctact gattgtacaa gatttaaaca tgaatgtaag aaattaaatc ttggttatcc attaaaattt gctactagta ataaatttac tcactttgcc aataaaaatt gggaagaaat aaaagaaatt atttatacat atcatggatt tattagtgca atgaaagtta ctgaatcatt atatagatat tcaggtggta tttataaatc tcaaagttgt actggacaaa ttactgacca tgttattact gttgatggtt atggagaatg tgatggacat aaattccttt gggtaagaaa ttcatgggga aatgattggg gagttgaagg tggacatttc aaaattgatt gggatactct ttgtggaatt aatggtattg atcattatga agatggttct caacttcaaa ataatttata tgttgaaatg gaattaggag atggtgatac tgctgattac aatcctcaag atggtgcagg tgaaaacaag caagacccaa ttggttgtgt tgttgttagt ccaactggtg ataatgataa caaaacaaca ggaattgtta ttactttatt cgtcttatta atgactttct tcatctaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa