SSGCID: Target Page

EnhiA.00232.a

glycerol kinase, putative

CENTER ID:	EnhiA.00232.a
ORGANISM:	Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE:	Amoebic dysentery
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EnhiA.00232.a.A1.GE27540	Full length( EnhiA.00232.a )		1	485

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:EHI_164850
RefSeq:	XP_655213.1
UniProt:	C4M1X8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKYILAIDQG TTSTRVILFD EKCQSIHTEQ EEFDSIFPHP GWVEQDPEVI YTSVVNLMKK CLVNTGINKE DIAAIGITNQ RETTVMWDKR TGKPIYNAIV WQSKQSGNET SYLMEKGYEE IFQSKTGLVL NPYFSASKIM WIFNNVEGAK ALAEEGVLLF GTIDTWIIYN LTGGQSHSTD ISNAARTLLF NIYEKKWDDE LLAKTNIPKS ILPIVKQSSD DFGIVSTIQE FNGIHITGVA GDQQASLFGH GSPIGGCKST YGTGCFVVKN IGDTIKEIPK GLLATVGWEI NGKITYALEG TVMTAGAALK WIRDIGILKD YNEISKIVTS KNGGVYFVPA FQGLGTPYWD DDVRGIIVGL TSGTGKGELV RATLESIALQ CAQVIEIMGK INEIKVDGGV SRSDCMLQFQ ADICNCTVIR LKEKEITAMG AAMLAGLYVH LFKEDELDSM RVIEHVFKPQ MSEEERKQIF SVWNKAVESA RSFVH

NT Sequence

ATGAAATATA TCCTTGCTAT TGATCAAGGT ACTACAAGTA CTAGAGTTAT TCTATTTGAT GAAAAGTGTC AGTCAATTCA TACAGAACAA GAAGAATTTG ATTCTATTTT TCCACATCCA GGTTGGGTTG AACAAGATCC TGAAGTAATT TATACTAGTG TTGTTAACTT AATGAAAAAA TGTCTTGTTA ATACTGGCAT TAATAAAGAA GACATTGCAG CTATTGGAAT AACTAATCAA AGAGAAACTA CAGTTATGTG GGATAAAAGA ACAGGAAAAC CTATTTACAA TGCAATAGTA TGGCAGTCTA AACAATCAGG AAATGAAACA TCTTATCTTA TGGAGAAAGG ATATGAAGAG ATTTTTCAGT CTAAAACTGG ACTAGTACTA AATCCTTATT TCAGTGCTAG TAAAATTATG TGGATATTTA ATAATGTAGA AGGTGCAAAG GCATTAGCTG AAGAAGGTGT TTTATTGTTT GGAACTATTG ACACATGGAT TATCTATAAT CTAACTGGAG GTCAAAGTCA TTCTACTGAT ATATCAAATG CAGCACGTAC TTTATTGTTT AATATTTATG AAAAGAAATG GGATGATGAG TTATTAGCAA AAACAAATAT TCCAAAGTCT ATTCTCCCAA TAGTTAAACA ATCCTCTGAT GATTTTGGTA TTGTTTCTAC TATTCAGGAA TTTAATGGGA TTCATATTAC TGGAGTTGCT GGAGATCAAC AAGCATCACT TTTTGGACAT GGAAGTCCTA TAGGAGGATG TAAATCAACA TATGGAACTG GGTGTTTCGT TGTAAAAAAT ATTGGAGATA CAATTAAAGA AATACCAAAA GGATTATTAG CAACTGTTGG ATGGGAAATT AATGGAAAGA TAACATATGC TCTTGAAGGC ACAGTTATGA CTGCTGGAGC AGCACTAAAA TGGATAAGGG ATATAGGAAT ATTAAAAGAC TACAATGAAA TATCAAAAAT TGTTACTTCC AAAAATGGTG GAGTTTATTT TGTACCAGCA TTTCAAGGAC TTGGGACACC TTATTGGGAT GATGATGTTC GTGGCATAAT TGTTGGACTA ACTAGTGGTA CTGGAAAAGG TGAATTAGTT CGAGCTACTC TAGAAAGTAT TGCTTTACAA TGTGCTCAAG TAATTGAAAT TATGGGAAAA ATTAATGAAA TTAAAGTGGA TGGAGGGGTT TCAAGGTCTG ATTGTATGCT CCAGTTTCAA GCAGATATAT GCAATTGTAC TGTTATTAGA CTGAAAGAGA AAGAAATTAC TGCAATGGGA GCAGCTATGT TAGCTGGGTT ATATGTTCAT TTATTTAAAG AAGATGAATT AGACTCAATG AGAGTAATTG AACATGTATT TAAACCTCAA ATGAGTGAAG AAGAAAGAAA GCAAATATTT TCAGTTTGGA ATAAAGCAGT AGAATCAGCA CGTTCATTTG TCCAT

Details for EnhiA.00232.a.A1.GE27540

HARVESTED ON:	7/21/2009
SEQUENCED ON:	7/28/2009
EXPECTED MW:	55kDa
OBSERVED MW:	56kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMKYILAIDQ GTTSTRVILF DEKCQSIHTE QEEFDSIFPH PGWVEQDPEV IYTSVVNLMK KCLVNTGINK EDIAAIGITN QRETTVMWDK RTGKPIYNAI VWQSKQSGNE TSYLMEKGYE EIFQSKTGLV LNPYFSASKI MWIFNNVEGA KALAEEGVLL FGTIDTWIIY NLTGGQSHST DISNAARTLL FNIYEKKWDD ELLAKTNIPK SILPIVKQSS DDFGIVSTIQ EFNGIHITGV AGDQQASLFG HGSPIGGCKS TYGTGCFVVK NIGDTIKEIP KGLLATVGWE INGKITYALE GTVMTAGAAL KWIRDIGILK DYNEISKIVT SKNGGVYFVP AFQGLGTPYW DDDVRGIIVG LTSGTGKGEL VRATLESIAL QCAQVIEIMG KINEIKVDGG VSRSDCMLQF QADICNCTVI RLKEKEITAM GAAMLAGLYV HLFKEDELDS MRVIEHVFKP QMSEEERKQI FSVWNKAVES ARSFVH

Validated NT Sequence

ttattaatgg acaaatgaac gtgctgattc tactgcttta ttccaaactg aaaatatttg ctttctttct tcttcactca tttgaggttt aaatacatgt tcaattactc tcattgagtc taattcatct tctttaaata aatgaacata taacccagct aacatagctg ctcccattgc agtaatttct ttctctttca gtctaataac agtacaattg catatatctg cttgaaactg gagcatacaa tcagaccttg aaacccctcc atccacttta atttcattaa tttttcccat aatttcaatt acttgagcac attgtaaagc aatactttct agagtagctc gaactaattc accttttcca gtaccactag ttagtccaac aattatgcca cgaacatcat catcccaata aggtgtccca agtccttgaa atgctggtac aaaataaact ccaccatttt tggaagtaac aatttttgat atttcattgt agtcttttaa tattcctata tcccttatcc attttagtgc tgctccagca gtcataactg tgccttcaag agcatatgtt atctttccat taatttccca tccaacagtt gctaataatc cttttggtat ttctttaatt gtatctccaa tattttttac aacgaaacac ccagttccat atgttgattt acatcctcct ataggacttc catgtccaaa aagtgatgct tgttgatctc cagcaactcc agtaatatga atcccattaa attcctgaat agtagaaaca ataccaaaat catcagagga ttgtttaact attgggagaa tagactttgg aatatttgtt tttgctaata actcatcatc ccatttcttt tcataaatat taaacaataa agtacgtgct gcatttgata tatcagtaga atgactttga cctccagtta gattatagat aatccatgtg tcaatagttc caaacaataa aacaccttct tcagctaatg cctttgcacc ttctacatta ttaaatatcc acataatttt actagcactg aaataaggat ttagtactag tccagtttta gactgaaaaa tctcttcata tcctttctcc ataagataag atgtttcatt tcctgattgt ttagactgcc atactattgc attgtaaata ggttttcctg ttcttttatc ccacataact gtagtttctc tttgattagt tattccaata gctgcaatgt cttctttatt aatgccagta ttaacaagac attttttcat taagttaaca acactagtat aaattacttc aggatcttgt tcaacccaac ctggatgtgg aaaaatagaa tcaaattctt cttgttctgt atgaattgac tgacactttt catcaaatag aataactcta gtacttgtag taccttgatc aatagcaagg atatatttca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaatatatc cttgctattg atcaaggtac tacaagtact agagttattc tatttgatga aaagtgtcag tcaattcata cagaacaaga agaatttgat tctatttttc cacatccagg ttgggttgaa caagatcctg aagtaattta tactagtgtt gttaacttaa tgaaaaaatg tcttgttaat actggcatta ataaagaaga cattgcagct attggaataa ctaatcaaag agaaactaca gttatgtggg ataaaagaac aggaaaacct atttacaatg caatagtatg gcagtctaaa caatcaggaa atgaaacatc ttatcttatg gagaaaggat atgaagagat ttttcagtct aaaactggac tagtactaaa tccttatttc agtgctagta aaattatgtg gatatttaat aatgtagaag gtgcaaaggc attagctgaa gaaggtgttt tattgtttgg aactattgac acatggatta tctataatct aactggaggt caaagtcatt ctactgatat atcaaatgca gcacgtactt tattgtttaa tatttatgaa aagaaatggg atgatgagtt attagcaaaa acaaatattc caaagtctat tctcccaata gttaaacaat cctctgatga ttttggtatt gtttctacta ttcaggaatt taatgggatt catattactg gagttgctgg agatcaacaa gcatcacttt ttggacatgg aagtcctata ggaggatgta aatcaacata tggaactggg tgtttcgttg taaaaaatat tggagataca attaaagaaa taccaaaagg attattagca actgttggat gggaaattaa tggaaagata acatatgctc ttgaaggcac agttatgact gctggagcag cactaaaatg gataagggat ataggaatat taaaagacta caatgaaata tcaaaaattg ttacttccaa aaatggtgga gtttattttg taccagcatt tcaaggactt gggacacctt attgggatga tgatgttcgt ggcataattg ttggactaac tagtggtact ggaaaaggtg aattagttcg agctactcta gaaagtattg ctttacaatg tgctcaagta attgaaatta tgggaaaaat taatgaaatt aaagtggatg gaggggtttc aaggtctgat tgtatgctcc agtttcaagc agatatatgc aattgtactg ttattagact gaaagagaaa gaaattactg caatgggagc agctatgtta gctgggttat atgttcattt atttaaagaa gatgaattag actcaatgag agtaattgaa catgtattta aacctcaaat gagtgaagaa gaaagaaagc aaatattttc agtttggaat aaagcagtag aatcagcacg ttcatttgtc cataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa