EnhiA.00231.a

threonine dehydratase, putative

CENTER ID: EnhiA.00231.a
ORGANISM: Entamoeba histolytica HM-1:IMSS
ASSOCIATED DISEASE: Amoebic dysentery
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.00231.a.A1.GE26010 Full length( EnhiA.00231.a ) 1 429
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EnhiA.00231.a.A1.PS00186 Full length( EnhiA.00231.a ) 1 429

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:EHI_049910
RefSeq: XP_657171.1
UniProt: C4LUI9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDVEVPALA LRDASFSEIH IGEGDEQVLE QIFLSDVYTA KHVISDHIHH TPLDSNASIS ADIGMDVYFK LENMQKSGSF KVRGVMNKFN SLTLAEKKRG VIAASSGNHG MAVAYCGKQL GVPTTIIVPN FTNPRRAETM KRYGATVILH GVTVAEAEAY AKEMSTEKRL AYIEARDDVS IIAGDGTVGL EILRDLPDVD AIIAPIGSGS LLCGLALAVK TLDPRVKVYG VQNEKGSSAY LSKKHHVLCR THQNEDNELT DVVNTIGNLT LHMIESYVDD IITVDETQIT RAMMLLMERC KVIIEGAGAV ALAGLLSKKL PIEKGSKVAV IVSGGNVNMR LLNTVIDLGL INCERSLKLE VTAPDSPINL NELVSIIAEQ KAQLYDFQVK KNVGIGMCTI RFSVEVADKN HKQALGDALK AKGYEFVFV
NT Sequence
ATGAGTGACG TAGAAGTTCC TGCACTTGCT CTAAGAGATG CTTCTTTTAG TGAGATTCAT ATTGGAGAAG GAGACGAACA AGTTTTAGAA CAAATCTTCT TATCTGATGT TTATACAGCC AAACATGTTA TTAGTGATCA CATTCATCAT ACTCCATTAG ATTCTAATGC TAGCATTTCA GCAGATATTG GAATGGATGT TTATTTTAAA TTAGAAAATA TGCAAAAAAG TGGTTCATTT AAAGTACGAG GTGTTATGAA CAAATTTAAT TCATTAACTC TTGCTGAAAA GAAAAGAGGA GTAATTGCTG CTTCAAGTGG TAATCATGGT ATGGCTGTTG CTTATTGTGG TAAACAATTA GGTGTTCCAA CTACTATCAT TGTTCCAAAT TTTACAAACC CAAGAAGAGC AGAAACAATG AAAAGATATG GTGCTACTGT TATTTTACAT GGAGTTACTG TTGCAGAAGC TGAAGCTTAT GCCAAAGAAA TGTCAACTGA AAAAAGACTT GCTTACATTG AAGCTCGTGA TGACGTTTCT ATTATTGCTG GTGATGGAAC AGTTGGTCTT GAGATTTTAA GAGATTTACC AGATGTAGAT GCTATTATTG CACCAATTGG ATCAGGAAGT TTACTTTGTG GATTAGCTTT AGCTGTTAAA ACCCTTGACC CAAGAGTTAA AGTTTATGGT GTTCAAAATG AAAAAGGGTC TTCAGCTTAT CTTTCTAAAA AACATCATGT TCTTTGTAGA ACTCATCAAA ATGAAGATAA TGAATTAACT GATGTTGTTA ATACTATTGG AAATTTAACT CTTCACATGA TAGAATCTTA TGTTGATGAT ATTATTACAG TTGATGAAAC CCAAATCACA AGAGCTATGA TGTTATTAAT GGAGAGATGT AAAGTTATTA TTGAAGGGGC TGGTGCTGTT GCTTTAGCTG GTCTCCTTTC TAAAAAACTT CCAATTGAAA AAGGTTCAAA AGTTGCAGTT ATTGTTTCTG GTGGTAATGT TAATATGAGA TTATTAAATA CTGTTATTGA TCTTGGATTA ATTAATTGTG AACGTTCACT TAAATTAGAA GTTACTGCTC CAGATTCACC AATTAATCTC AATGAATTAG TTAGTATTAT TGCTGAACAA AAAGCTCAAT TATATGATTT CCAAGTTAAA AAGAATGTAG GTATTGGAAT GTGTACCATC AGATTCTCAG TAGAAGTTGC TGATAAAAAC CATAAACAAG CTCTTGGTGA CGCATTAAAA GCTAAAGGTT ATGAATTTGT CTTTGTC
Details for EnhiA.00231.a.A1.GE26010
HARVESTED ON: 1/9/2009
SEQUENCED ON: 7/28/2010
EXPECTED MW: 48kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 96
PERCENT COVERAGE: 63
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSDVEVPAL ALRDASFSEI HIGEGDEQVL EQIFLSDVYT AKHVISDHIH HTPLDSNASI SADIGMDVYF KLENMQKSGS FKVRGVMNKF NSLTLAEKKR GVIAASSGNH GMAVAYCGKQ LGVPTTIIVP NFTNPRRAET MKRYGATVIL HGVTVAEAEA YAKEMSTEKR LAYIEARDDV SIIAGDGTVG LEILRDLPXV DAIIAPIGSG SLLCXLAXAV KTLDPRVKVY GVQNEKGXSA YLSXKHHVLC RTXQXXDXXL TDVVNTI
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagtg acgtagaagt tcctgcactt gctctaagag atgcttcttt tagtgagatt catattggag aaggagacga acaagtttta gaacaaatct tcttatctga tgtttataca gccaaacatg ttattagtga tcacattcat catactccat tagattctaa tgctagcatt tcagcagata ttggaatgga tgtttatttt aaattagaaa atatgcaaaa aagtggttca tttaaagtac gaggtgttat gaacaaattt aattcattaa ctcttgctga aaagaaaaga ggagtaattg ctgcttcaag tggtaatcat ggtatggctg ttgcttattg tggtaaacaa ttaggtgttc caactactat cattgttcca aattttacaa acccaagaag agcagaaaca atgaaaagat atggtgctac tgttatttta catggagtta ctgttgcaga agctgaagct tatgccaaag aaatgtcaac tgaaaaaaga cttgcttaca ttgaagctcg tgatgacgtt tctattattg ctggtgatgg aacagttggt cttgagattt taagagattt accagangta gatgctatta ttgcaccaat tggatcagga agtttacttt gtgnattagc ttnagctgtt aaaacccttg acccaagagt taaagtttat ggtgttcaaa atgaaaaagg gncttcagct tatctttctn aaaaacatca tgttctttgt agaactcntc aanatgnaga taangantta actgatgttg ttaatactat tg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSDVEVPAL ALRDASFSEI HIGEGDEQVL EQIFLSDVYT AKHVISDHIH HTPLDSNASI SADIGMDVYF KLENMQKSGS FKVRGVMNKF NSLTLAEKKR GVIAASSGNH GMAVAYCGKQ LGVPTTIIVP NFTNPRRAET MKRYGATVIL HGVTVAEAEA YAKEMSTEKR LAYIEARDDV SIIAGDGTVG LEILRDLPDV DAIIAPIGSG SLLCGLALAV KTLDPRVKVY GVQNEKGSSA YLSKKHHVLC RTHQNEDNEL TDVVNTIGNL TLHMIESYVD DIITVDETQI TRAMMLLMER CKVIIEGAGA VALAGLLSKK LPIEKGSKVA VIVSGGNVNM RLLNTVIDLG LINCERSLKL EVTAPDSPIN LNELVSIIAE QKAQLYDFQV KKNVGIGMCT IRFSVEVADK NHKQALGDAL KAKGYEFVFV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtgacgta gaagttcctg cacttgctct aagagatgct tcttttagtg agattcatat tggagaagga gacgaacaag ttttagaaca aatcttctta tctgatgttt atacagccaa acatgttatt agtgatcaca ttcatcatac tccattagat tctaatgcta gcatttcagc agatattgga atggatgttt attttaaatt agaaaatatg caaaaaagtg gttcatttaa agtacgaggt gttatgaaca aatttaattc attaactctt gctgaaaaga aaagaggagt aattgctgct tcaagtggta atcatggtat ggctgttgct tattgtggta aacaattagg tgttccaact actatcattg ttccaaattt tacaaaccca agaagagcag aaacaatgaa aagatatggt gctactgtta ttttacatgg agttactgtt gcagaagctg aagcttatgc caaagaaatg tcaactgaaa aaagacttgc ttacattgaa gctcgtgatg acgtttctat tattgctggt gatggaacag ttggtcttga gattttaaga gatttaccag atgtagatgc tattattgca ccaattggat caggaagttt actttgtgga ttagctttag ctgttaaaac ccttgaccca agagttaaag tttatggtgt tcaaaatgaa aaagggtctt cagcttatct ttctaaaaaa catcatgttc tttgtagaac tcatcaaaat gaagataatg aattaactga tgttgttaat actattggaa atttaactct tcacatgata gaatcttatg ttgatgatat tattacagtt gatgaaaccc aaatcacaag agctatgatg ttattaatgg agagatgtaa agttattatt gaaggggctg gtgctgttgc tttagctggt ctcctttcta aaaaacttcc aattgaaaaa ggttcaaaag ttgcagttat tgtttctggt ggtaatgtta atatgagatt attaaatact gttattgatc ttggattaat taattgtgaa cgttcactta aattagaagt tactgctcca gattcaccaa ttaatctcaa tgaattagtt agtattattg ctgaacaaaa agctcaatta tatgatttcc aagttaaaaa gaatgtaggt attggaatgt gtaccatcag attctcagta gaagttgctg ataaaaacca taaacaagct cttggtgacg cattaaaagc taaaggttat gaatttgtct ttgtcaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for EnhiA.00231.a.A1.PS00186
PURIFICATION DATe: 3/19/2009
CONCENTRATION: 23.49mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 50µl
PERCENT IDENTITY: 96
PERCENT COVERAGE: 63
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSDVEVPAL ALRDASFSEI HIGEGDEQVL EQIFLSDVYT AKHVISDHIH HTPLDSNASI SADIGMDVYF KLENMQKSGS FKVRGVMNKF NSLTLAEKKR GVIAASSGNH GMAVAYCGKQ LGVPTTIIVP NFTNPRRAET MKRYGATVIL HGVTVAEAEA YAKEMSTEKR LAYIEARDDV SIIAGDGTVG LEILRDLPXV DAIIAPIGSG SLLCXLAXAV KTLDPRVKVY GVQNEKGXSA YLSXKHHVLC RTXQXXDXXL TDVVNTI
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagtg acgtagaagt tcctgcactt gctctaagag atgcttcttt tagtgagatt catattggag aaggagacga acaagtttta gaacaaatct tcttatctga tgtttataca gccaaacatg ttattagtga tcacattcat catactccat tagattctaa tgctagcatt tcagcagata ttggaatgga tgtttatttt aaattagaaa atatgcaaaa aagtggttca tttaaagtac gaggtgttat gaacaaattt aattcattaa ctcttgctga aaagaaaaga ggagtaattg ctgcttcaag tggtaatcat ggtatggctg ttgcttattg tggtaaacaa ttaggtgttc caactactat cattgttcca aattttacaa acccaagaag agcagaaaca atgaaaagat atggtgctac tgttatttta catggagtta ctgttgcaga agctgaagct tatgccaaag aaatgtcaac tgaaaaaaga cttgcttaca ttgaagctcg tgatgacgtt tctattattg ctggtgatgg aacagttggt cttgagattt taagagattt accagangta gatgctatta ttgcaccaat tggatcagga agtttacttt gtgnattagc ttnagctgtt aaaacccttg acccaagagt taaagtttat ggtgttcaaa atgaaaaagg gncttcagct tatctttctn aaaaacatca tgttctttgt agaactcntc aanatgnaga taangantta actgatgttg ttaatactat tg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSDVEVPAL ALRDASFSEI HIGEGDEQVL EQIFLSDVYT AKHVISDHIH HTPLDSNASI SADIGMDVYF KLENMQKSGS FKVRGVMNKF NSLTLAEKKR GVIAASSGNH GMAVAYCGKQ LGVPTTIIVP NFTNPRRAET MKRYGATVIL HGVTVAEAEA YAKEMSTEKR LAYIEARDDV SIIAGDGTVG LEILRDLPDV DAIIAPIGSG SLLCGLALAV KTLDPRVKVY GVQNEKGSSA YLSKKHHVLC RTHQNEDNEL TDVVNTIGNL TLHMIESYVD DIITVDETQI TRAMMLLMER CKVIIEGAGA VALAGLLSKK LPIEKGSKVA VIVSGGNVNM RLLNTVIDLG LINCERSLKL EVTAPDSPIN LNELVSIIAE QKAQLYDFQV KKNVGIGMCT IRFSVEVADK NHKQALGDAL KAKGYEFVFV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGTGACGTA GAAGTTCCTG CACTTGCTCT AAGAGATGCT TCTTTTAGTG AGATTCATAT TGGAGAAGGA GACGAACAAG TTTTAGAACA AATCTTCTTA TCTGATGTTT ATACAGCCAA ACATGTTATT AGTGATCACA TTCATCATAC TCCATTAGAT TCTAATGCTA GCATTTCAGC AGATATTGGA ATGGATGTTT ATTTTAAATT AGAAAATATG CAAAAAAGTG GTTCATTTAA AGTACGAGGT GTTATGAACA AATTTAATTC ATTAACTCTT GCTGAAAAGA AAAGAGGAGT AATTGCTGCT TCAAGTGGTA ATCATGGTAT GGCTGTTGCT TATTGTGGTA AACAATTAGG TGTTCCAACT ACTATCATTG TTCCAAATTT TACAAACCCA AGAAGAGCAG AAACAATGAA AAGATATGGT GCTACTGTTA TTTTACATGG AGTTACTGTT GCAGAAGCTG AAGCTTATGC CAAAGAAATG TCAACTGAAA AAAGACTTGC TTACATTGAA GCTCGTGATG ACGTTTCTAT TATTGCTGGT GATGGAACAG TTGGTCTTGA GATTTTAAGA GATTTACCAG ATGTAGATGC TATTATTGCA CCAATTGGAT CAGGAAGTTT ACTTTGTGGA TTAGCTTTAG CTGTTAAAAC CCTTGACCCA AGAGTTAAAG TTTATGGTGT TCAAAATGAA AAAGGGTCTT CAGCTTATCT TTCTAAAAAA CATCATGTTC TTTGTAGAAC TCATCAAAAT GAAGATAATG AATTAACTGA TGTTGTTAAT ACTATTGGAA ATTTAACTCT TCACATGATA GAATCTTATG TTGATGATAT TATTACAGTT GATGAAACCC AAATCACAAG AGCTATGATG TTATTAATGG AGAGATGTAA AGTTATTATT GAAGGGGCTG GTGCTGTTGC TTTAGCTGGT CTCCTTTCTA AAAAACTTCC AATTGAAAAA GGTTCAAAAG TTGCAGTTAT TGTTTCTGGT GGTAATGTTA ATATGAGATT ATTAAATACT GTTATTGATC TTGGATTAAT TAATTGTGAA CGTTCACTTA AATTAGAAGT TACTGCTCCA GATTCACCAA TTAATCTCAA TGAATTAGTT AGTATTATTG CTGAACAAAA AGCTCAATTA TATGATTTCC AAGTTAAAAA GAATGTAGGT ATTGGAATGT GTACCATCAG ATTCTCAGTA GAAGTTGCTG ATAAAAACCA TAAACAAGCT CTTGGTGACG CATTAAAAGC TAAAGGTTAT GAATTTGTCT TTGTCAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA