EncuA.18809.a

3-HYDROXY-3-METHYLGLUTARYL CoA REDUCTASE

CENTER ID: EncuA.18809.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.18809.a.B2.GE41341 soluble domain 1 305
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU10_1720
UniProt: Q8SQZ9
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGDANRKANE RANRLLKNIR EETGTEIKGI GSEEDYSDVI NRCCENLIGY KKVPLGVSNR GVLINGRMFF IPIATTEGAL VASMCRGIKL INACGGVKGY AENVGITRGF IMRFESFEEA MRFYQWIKMD EAVEDLKRIG NEGSRYMKIS KIESKHVVGE DVYVKVYGYS GDAMGMNMIT KACTQISAEI SRRFPHSSLV CISSNTCTDK KWSAENYCNG RGRRIFMNMK IDDKSCREIL GVGIRQVLDV YHAKVVIGGS LVLGGFNCQA ANYVAGMFLA LGQDLGQVIE SSNCVLSMKR STDGDLSVSL FMPSVVVGVI GGGTHLEPSK SFLKQFYSGS GEYIITNRDD DKSLAPGYLG LAVGAAVLGG ELSLLGSLAN NTLMESHLRL NREECRGG
NT Sequence
atgggcgacg ccaacagaaa agctaacgag cgagcaaata gattgctgaa aaacataagg gaggagacag gaactgaaat aaagggaatt ggctctgaag aggactattc ggatgttatc aacagatgct gtgaaaacct gattggatat aagaaggtgc ctttgggtgt ttcgaatagg ggagtgttaa taaacggccg catgtttttt attccgatag caacgaccga gggtgcgcta gttgcatcga tgtgccgggg aattaaactt ataaatgcat gtggaggagt taaaggatat gcagagaatg ttgggattac aagaggattt atcatgagat ttgagagctt tgaagaggcg atgagattct accaatggat aaagatggat gaggcagtcg aagatctcaa aaggatagga aatgaaggaa gcaggtatat gaagattagt aagattgaat ccaagcatgt tgtcggtgaa gatgtgtatg taaaggtgta tggatactct ggagatgcca tggggatgaa tatgataaca aaggcatgca cccagatatc tgctgaaata tctcgaagat tcccccactc cagccttgtg tgcataagct cgaacacgtg cactgacaag aagtggtctg ccgagaacta ctgcaatgga agagggagaa ggatattcat gaatatgaaa atagatgaca aaagctgcag agagatactt ggggttggca taagacaggt tctggatgtg tatcatgcaa aggttgttat aggaggatct cttgtgctgg gagggtttaa ctgccaagca gctaattatg ttgcaggaat gtttctagct ctagggcagg atctggggca ggttattgag agcagcaact gtgtattgag catgaagaga tccacggatg gagacttaag cgtttcttta ttcatgcctt cggttgtggt gggagttatt ggaggaggca cgcatctaga accttcaaaa agctttctaa agcagttcta tagtgggagc ggcgaatata taataacgaa cagggacgac gacaagtcgt tggcacctgg atacctgggt ctggctgtcg gggctgcagt gcttggaggt gagctgtctc tcttagggtc gctagcaaac aataccttaa tggaaagcca tctgagattg aatcgagaag aatgcagagg tggctag
Details for EncuA.18809.a.B2.GE41341
HARVESTED ON: data unavailable
SEQUENCED ON: 4/10/2017
EXPECTED MW: 35kDa
OBSERVED MW: 0kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHHM GDANRKANER ANRLLKNIRE ETGTEIKGIG SEEDYSDVIN RCCENLIGYK KVPLGVSNRG VLINGRMFFI PIATTEGALV ASMCRGIKLI NACGGVKGYA ENVGITRGFI MRFESFEEAM RFYQWIKMDE AVEDLKRIGN EGSRYMKISK IESKHVVGED VYVKVYGYSG DAMGMNMITK ACTQISAEIS RRFPHSSLVC ISSNTCTDKK WSAENYCNGR GRRIFMNMKI DDKSCREILG VGIRQVLDVY HAKVVIGGSL VLGGFNCQAA NYVAGMFLAL GQDLGQVIES SNCVLS
Validated NT Sequence
tgctcaatac acagttgctg ctctcaataa cctgccccag atcctgccct agagctagaa acattcctgc aacataatta gctgcttggc agttaaaccc tcccagcaca agagatcctc ctataacaac ctttgcatga tacacatcca gaacctgtct tatgccaacc ccaagtatct ctctgcagct tttgtcatct attttcatat tcatgaatat ccttctccct cttccattgc agtagttctc ggcagaccac ttcttgtcag tgcacgtgtt cgagcttatg cacacaaggc tggagtgggg gaatcttcga gatatttcag cagatatctg ggtgcatgcc tttgttatca tattcatccc catggcatct ccagagtatc catacacctt tacatacaca tcttcaccga caacatgctt ggattcaatc ttactaatct tcatatacct gcttccttca tttcctatcc ttttgagatc ttcgactgcc tcatccatct ttatccattg gtagaatctc atcgcctctt caaagctctc aaatctcatg ataaatcctc ttgtaatccc aacattctct gcatatcctt taactcctcc acatgcattt ataagtttaa ttccccggca catcgatgca actagcgcac cctcggtcgt tgctatcgga ataaaaaaca tgcggccgtt tattaacact cccctattcg aaacacccaa aggcaccttc ttatatccaa tcaggttttc acagcatctg ttgataacat ccgaatagtc ctcttcagag ccaattccct ttatttcagt tcctgtctcc tcccttatgt ttttcagcaa tctatttgct cgctcgttag cttttctgtt ggcgtcgccc atatggtggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMG DANRKANERA NRLLKNIREE TGTEIKGIGS EEDYSDVINR CCENLIGYKK VPLGVSNRGV LINGRMFFIP IATTEGALVA SMCRGIKLIN ACGGVKGYAE NVGITRGFIM RFESFEEAMR FYQWIKMDEA VEDLKRIGNE GSRYMKISKI ESKHVVGEDV YVKVYGYSGD AMGMNMITKA CTQISAEISR RFPHSSLVCI SSNTCTDKKW SAENYCNGRG RRIFMNMKID DKSCREILGV GIRQVLDVYH AKVVIGGSLV LGGFNCQAAN YVAGMFLALG QDLGQVIESS NCVLSMKRST DGD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggcg acgccaacag aaaagctaac gagcgagcaa atagattgct gaaaaacata agggaggaga caggaactga aataaaggga attggctctg aagaggacta ttcggatgtt atcaacagat gctgtgaaaa cctgattgga tataagaagg tgcctttggg tgtttcgaat aggggagtgt taataaacgg ccgcatgttt tttattccga tagcaacgac cgagggtgcg ctagttgcat cgatgtgccg gggaattaaa cttataaatg catgtggagg agttaaagga tatgcagaga atgttgggat tacaagagga tttatcatga gatttgagag ctttgaagag gcgatgagat tctaccaatg gataaagatg gatgaggcag tcgaagatct caaaaggata ggaaatgaag gaagcaggta tatgaagatt agtaagattg aatccaagca tgttgtcggt gaagatgtgt atgtaaaggt gtatggatac tctggagatg ccatggggat gaatatgata acaaaggcat gcacccagat atctgctgaa atatctcgaa gattccccca ctccagcctt gtgtgcataa gctcgaacac gtgcactgac aagaagtggt ctgccgagaa ctactgcaat ggaagaggga gaaggatatt catgaatatg aaaatagatg acaaaagctg cagagagata cttggggttg gcataagaca ggttctggat gtgtatcatg caaaggttgt tataggagga tctcttgtgc tgggagggtt taactgccaa gcagctaatt atgttgcagg aatgtttcta gctctagggc aggatctggg gcaggttatt gagagcagca actgtgtatt gagcatgaag agatccacgg atggagactg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat