EncuA.17854.a

Chitin synthase 1

CENTER ID: EncuA.17854.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.17854.a.A2.GE32220 soluble domain 1 85 240
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU01_1390
RefSeq: XP_965977.1
UniProt: Q8SSI7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLSQGEILRN PSRTRLQRPP KSRSERKGWW YRVTIFLTCL IPNFMLRCFG MTTPEVQHAW REKVALCICI FFCWIILGFT TYGMNTIICK GSNQYVASRL KRDAFDGNTV IANGGIYYTD DEYAFGENHT YAFEKKSGAC KLAFGRQLPS GDEDIDDLER INDIYWDWGD IMSKGMIVVG NKVYDPSYCT EPLFEEFNRK YAGTEGKPDF DTDEWRCYED MFYAGKVATK TPGCLLADTM FWITTISIFG LIITKFLLGF FYSWYAKRRP KPSPKITPCI LLVTCYSEGK DGIKNTLDSL CKQDYGYDYK LIVVICDGNI TGSGNSMSTP DIVLGLSDVD RRAEPKGYIS LTHGTKRYNR AKVHAGYYHV REEKKSRRYR CWPCFGRQAD SSEVENYKTR ILVINKCGNP SETFKAGNRG KRDSQVILMS FFSKLIYGDR MTELDFEIYQ KMKFLMPHIE PEDFECILMV DADTIVKPDA LSIMVNVFET DQKVIGMCGE TMILNKFESW VTMIQVFEYY ISHHLSKAFE SVFGGVTCLP GCFCMYRIKI VTNQQGQLLS GPSKSRASVP RFSSMKSILS SSLEKSLCLP ILANPAIINA YSVLEVKTLH QKNLLHLGED RYLTTLLLKT FYRRKLVFIP AAKCETYVPG EFSVLLSQRR RWINSTIHNL FELVQVNNLC GAFCFSMQLV VVMELFGTLV LPAAIIFTFV MIAVSILIEP AWVPLIMLVG IFGLPAVLIL ITTMEIQYVF WCLVYILSIP IWNFVLPTYA FWHFDNFSWG DTRKVDGEGK EDEEGEFDHT KIRIRELEEF LSEANK
NT Sequence
atgctatctc agggagaaat actaaggaat ccgtccagaa caaggcttca gaggcctcca aaaagtaggt ctgagaggaa aggatggtgg tatagggtca ctatatttct aacctgcctt attccaaatt ttatgctgag gtgttttgga atgacaacgc cagaggtcca gcatgcatgg agagaaaagg tggctctgtg catctgtata tttttctgct ggattatact gggctttacg acctatggaa tgaacaccat tatttgtaag ggaagtaatc aatatgttgc atcgagactc aagagagatg cattcgatgg aaatactgtt atagcaaatg gcgggatcta ctatacggat gatgaatatg cttttgggga aaaccacaca tatgcatttg aaaagaaaag cggtgcatgt aagcttgcgt ttggaagaca gctccctagt ggagatgaag acattgatga tttggagaga ataaacgata tctactggga ttggggggat ataatgagta aagggatgat agttgttggc aacaaggttt acgacccatc atattgcaca gagcctttgt ttgaagagtt caatcggaag tatgctggaa cagagggaaa acctgatttc gacacggatg aatggaggtg ctatgaggac atgttttatg ctgggaaggt tgccacaaag acacctgggt gtcttcttgc ggatacgatg ttctggatca caaccatttc gatttttggg ctgattatta caaagttctt actgggattt ttctactcct ggtatgcgaa gagaaggccg aaacccagcc caaagataac gccctgcata ttgctagtaa catgctatag tgaaggcaaa gacgggataa aaaacactct ggacagtctt tgcaagcagg attatggata cgactacaag ttgattgtag tgatctgtga tggaaatata actgggtctg ggaatagcat gagcactccg gatatagttt tgggtctgtc tgatgtggac agaagggcag agcccaaggg ttacatatct cttacccatg gaaccaagcg ctataaccga gccaaagtgc atgcgggata ctatcatgtg agggaggaga aaaagagtag gcgatacaga tgctggcctt gctttggaag gcaggcagac tccagtgaag ttgagaatta taagacaagg attcttgtaa tcaataagtg tggaaatccc tcggagacat tcaaagctgg aaacagagga aagagagata gccaggtaat cttgatgtcg tttttttcga agttaattta tggagacaga atgacggagc tggattttga gatctaccaa aagatgaaat ttttaatgcc tcacattgag ccggaggact ttgaatgtat actgatggtt gatgcagaca cgatagttaa gcctgatgct ctttcgatta tggtgaatgt atttgaaacg gaccagaagg ttatagggat gtgtggagag acgatgatcc tgaataagtt tgagagctgg gttaccatga tacaagtttt tgaatactat atcagtcacc atcttagcaa agcatttgag tctgtgtttg gtggtgttac gtgtcttccg gggtgcttct gcatgtatag aataaagata gttacgaacc aacaaggaca gctgttgtcg gggccgtcaa agtctcgagc aagtgtaccg agatttagca gcatgaagtc cattctgagc tcgtctttgg aaaagagcct ctgccttccc attctggcaa accctgcgat cataaatgcc tactcggttc ttgaggtaaa gactctgcac caaaagaatc ttttgcattt gggagaagat aggtatttaa caaccctatt gctgaagact ttctatagga ggaagcttgt gtttattcca gctgccaaat gcgaaacata tgttcccggg gaattcagtg ttcttcttag tcaaaggaga agatggatca actcgactat acacaacttg tttgagttgg tccaggtcaa caacctgtgc ggagccttct gcttttcgat gcagcttgtt gtggtgatgg aactgtttgg aacgctggtg ttgccggctg ctataatttt tacatttgtt atgattgctg tttcgatctt gattgagcct gcatgggttc ctttgatcat gctggtcggc atttttggtc ttcctgctgt actgatattg attactacga tggagatcca gtatgtgttt tggtgcttgg tttacatact gtctattccg atctggaact ttgtccttcc cacgtatgcc ttctggcatt tcgataactt cagctgggga gataccagaa aagttgatgg ggagggaaag gaggatgagg agggtgaatt cgaccacaca aagatcagaa tccgagagct tgaagagttt cttagtgaag caaataaatg a
Details for EncuA.17854.a.A2.GE32220
HARVESTED ON: 7/28/2011
SEQUENCED ON: 8/3/2011
EXPECTED MW: 20kDa
OBSERVED MW: 20kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNTIICKGS NQYVASRLKR DAFDGNTVIA NGGIYYTDDE YAFGENLTYA FEKKSGACKL AFGRQLPSGD EDIDDLERIN DIYWDWGDIM SKGMIVVGNK VYDPSYCTEP LFEEFNRKYA GTEGKPDFDT DEWRCYEDMS YAGKVATKTP GCLLADTM
Validated NT Sequence
antttnttnn ntttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaca ccattatttg taagggaagt aatcaatatg ttgcatcgag actcaagaga gatgcattcg atggaaatac tgttatagca aatggcggga tctactatac ggatgatgaa tatgcttttg gggaaaacct cacatatgca tttgaaaaga aaagcggtgc atgtaagctt gcgtttggaa gacagctccc tagtggagat gaagacattg atgatttgga gagaataaac gatatctact gggattgggg ggatataatg agtaaaggga tgatagttgt tggcaacaag gtttacgacc catcatattg cacagagcct ttgtttgaag agttcaatcg gaagtatgct ggaacagagg gaaaacctga tttcgacacg gatgaatgga ggtgctatga ggacatgtct tatgctggga aggttgccac aaagacacct gggtgtcttc ttgcggatac gatgtaataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccnnn nnnnnnnnnn naaaaaaana aaannnnggn ncnnnnnncn ncannnnnnn ngnagcnnnc nnnnnnnann nncnnngngc gnncnnntnn nnnngaannn nnnnnnnntc nnnnnnnnnc nnctatnnaa nncttgnnnn tgcncntctg cnnnantnca tcntcnnnna ancgntcgcg agcggcaagn ncgtcnaann gctcngnnan ntcntcncgn ctcnnttgac gcncnangng ntgtancnnn nnnnncgtcn ncttccntcn cganngagnn nnnnnnnntt nttnnnnnnn cnnnnnnnna aggnnntnnn tntnnnnaac nnnngcnnnn aantnntnnn nnnnnncnnn nncnnnnang nnngnnnana annncnnnnn nnnnnnt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNTIICKGS NQYVASRLKR DAFDGNTVIA NGGIYYTDDE YAFGENHTYA FEKKSGACKL AFGRQLPSGD EDIDDLERIN DIYWDWGDIM SKGMIVVGNK VYDPSYCTEP LFEEFNRKYA GTEGKPDFDT DEWRCYEDMF YAGKVATKTP GCLLADTM
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaacaccatt atttgtaagg gaagtaatca atatgttgca tcgagactca agagagatgc attcgatgga aatactgtta tagcaaatgg cgggatctac tatacggatg atgaatatgc ttttggggaa aaccacacat atgcatttga aaagaaaagc ggtgcatgta agcttgcgtt tggaagacag ctccctagtg gagatgaaga cattgatgat ttggagagaa taaacgatat ctactgggat tggggggata taatgagtaa agggatgata gttgttggca acaaggttta cgacccatca tattgcacag agcctttgtt tgaagagttc aatcggaagt atgctggaac agagggaaaa cctgatttcg acacggatga atggaggtgc tatgaggaca tgttttatgc tgggaaggtt gccacaaaga cacctgggtg tcttcttgcg gatacgatgt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa