EncuA.17526.a

SIGNAL SEQUENCE RECEPTOR ALPHA SUBUNIT (TRANSLOCON-ASSOCIATED PROTEIN ALPHA SUBUNIT)

CENTER ID: EncuA.17526.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.17526.a.A1.GE30871 Full length( EncuA.17526.a ) 1 338
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.17526.a.A1.PS01162 Full length( EncuA.17526.a ) 1 338

External Resources

RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU04_1230
RefSeq: NP_584807.1
UniProt: Q8ST29

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVYPLSCRMD LEKEVTIQVN LMEPKEIDLR KKTKQKKRRQ RTDEYDYNDP FIEPFEGETE MVMIECNLED FFVYKGELPY SAKKVLSVHK AKEKSRLAKS SNTDSCPQEA GGKKEGTGGS AVSETPTKRR RGKIPKSDTK ICDCLAGLIK SEVDRLELSI QSESTEVERY VHLMILETFQ QERGIEFWHI AGPKATKRPS DEEMARCLRE KESRMDVLFD AIAREIRNYD LYSDDLSLFK GFQKEDFLFN ACEYFLLFIK ISFLSQKDMS FNRARKEAYG NILALFPDTC RNSTQAQYYL AKHISQTCGI RGLGAPSSGR HEGENAEDDV SSNLERME
NT Sequence
ATGGTCTATC CCCTTTCTTG CAGAATGGAT CTTGAAAAAG AGGTTACAAT CCAGGTGAAT CTCATGGAGC CGAAGGAGAT CGATCTTAGG AAGAAGACAA AACAAAAGAA AAGGAGGCAA AGGACGGACG AGTATGACTA CAACGACCCG TTCATAGAGC CCTTTGAGGG AGAGACGGAG ATGGTGATGA TAGAATGCAA CCTCGAGGAT TTCTTTGTGT ACAAGGGAGA GCTGCCATAT AGTGCCAAGA AGGTGCTGAG CGTGCACAAG GCAAAAGAAA AATCCAGGCT TGCAAAGAGC TCAAACACAG ACTCTTGTCC TCAGGAGGCC GGTGGAAAAA AAGAGGGGAC AGGGGGCTCG GCAGTCTCAG AGACACCTAC AAAGAGGAGA CGTGGTAAGA TCCCAAAGAG CGACACCAAA ATCTGTGACT GCCTTGCTGG CCTCATCAAG TCCGAGGTCG ACAGGCTAGA GCTGTCGATT CAGTCCGAAA GCACCGAGGT GGAACGCTAT GTACATCTGA TGATCCTTGA AACATTTCAG CAGGAAAGGG GAATTGAGTT CTGGCATATT GCAGGTCCTA AAGCCACCAA GAGGCCATCG GATGAAGAAA TGGCGAGATG CCTAAGAGAG AAGGAGTCAA GAATGGATGT TCTATTCGAT GCAATCGCTA GGGAGATCCG GAACTATGAC CTTTACTCAG ATGACCTCTC TCTGTTCAAA GGCTTCCAGA AAGAAGACTT CCTATTTAAC GCATGCGAGT ACTTTCTACT TTTTATTAAG ATTTCGTTTC TCAGCCAGAA AGATATGTCG TTTAATAGAG CAAGGAAAGA GGCCTATGGC AACATTCTTG CTCTTTTTCC CGACACATGC AGAAACTCAA CGCAGGCCCA GTACTACCTT GCAAAGCACA TCTCGCAAAC CTGTGGAATC AGAGGCCTTG GAGCGCCCAG CAGTGGGAGG CATGAGGGGG AGAATGCAGA GGACGATGTG TCCTCAAACT TGGAAAGAAT GGAA
Details for EncuA.17526.a.A1.GE30871
HARVESTED ON: 11/16/2010
SEQUENCED ON: 11/23/2010
EXPECTED MW: 41kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Validated AA Sequence
MVYPLSCRMD LEKEVTIQVN LMEPKEIDLR KKTKQKKRRQ RTDEYDYNDP FIEPFEGETE MVMIECNLED FFVYKGELPY SAKKVLSVHK AKEKSRLAKS SNTDSCPQEA GGKKEGTGGS AVSETPTKRR RGKIPKSDTK ICDCLAGLIK SEVDRLELSI QSESTEVERY VHLMILETFQ QERGIEFWHI AGPKATKRPS DEEMARCLRE KESRMDVLFD AIAREIRNYD LYSDDLSLFK GFQKEDFLFN ACEYFLLFIK ISFLSQKDMS FNRARKEAYG NILALFPDTC RNSTQAQYYL AKHISQTCGI RGLGAPSSGR HEGENAEDDV SSNLERM
Validated NT Sequence
ttccattctt tccaagtttg aggacacatc gtcctctgca ttctccccct catgcctccc actgctgggc gctccaaggc ctctgattcc acaggtttgc gagatgtgct ttgcaaggta gtactgggcc tgcgttgagt ttctgcatgt gtcgggaaaa agagcaagaa tgttgccata ggcctctttc cttgctctat taaacgacat atctttctgg ctgagaaacg aaatcttaat aaaaagtaga aagtactcgc atgcgttaaa taggaagtct tctttctgga agcctttgaa cagagagagg tcatctgagt aaaggtcata gttccggatc tccctagcga ttgcatcgaa tagaacatcc attcttgact ccttctctct taggcatctc gccatttctt catccgatgg cctcttggtg gctttaggac ctgcaatatg ccagaactca attccccttt cctgctgaaa tgtttcaagg atcatcagat gtacatagcg ttccacctcg gtgctttcgg actgaatcga cagctctagc ctgtcgacct cggacttgat gaggccagca aggcagtcac agattttggt gtcgctcttt gggatcttac cacgtctcct ctttgtaggt gtctctgaga ctgccgagcc ccctgtcccc tctttttttc caccggcctc ctgaggacaa gagtctgtgt ttgagctctt tgcaagcctg gatttttctt ttgccttgtg cacgctcagc accttcttgg cactatatgg cagctctccc ttgtacacaa agaaatcctc gaggttgcat tctatcatca ccatctccgt ctctccctca aagggctcta tgaacgggtc gttgtagtca tactcgtccg tcctttgcct ccttttcttt tgttttgtct tcttcctaag atcgatctcc ttcggctcca tgagattcac ctggattgta acctcttttt caagatccat tctgcaagaa aggggataga ccatcgaacc aggaccctgg gtctgagctt ccagggtacc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVYPLSCRM DLEKEVTIQV NLMEPKEIDL RKKTKQKKRR QRTDEYDYND PFIEPFEGET EMVMIECNLE DFFVYKGELP YSAKKVLSVH KAKEKSRLAK SSNTDSCPQE AGGKKEGTGG SAVSETPTKR RRGKIPKSDT KICDCLAGLI KSEVDRLELS IQSESTEVER YVHLMILETF QQERGIEFWH IAGPKATKRP SDEEMARCLR EKESRMDVLF DAIAREIRNY DLYSDDLSLF KGFQKEDFLF NACEYFLLFI KISFLSQKDM SFNRARKEAY GNILALFPDT CRNSTQAQYY LAKHISQTCG IRGLGAPSSG RHEGENAEDD VSSNLERME
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtctatccc ctttcttgca gaatggatct tgaaaaagag gttacaatcc aggtgaatct catggagccg aaggagatcg atcttaggaa gaagacaaaa caaaagaaaa ggaggcaaag gacggacgag tatgactaca acgacccgtt catagagccc tttgagggag agacggagat ggtgatgata gaatgcaacc tcgaggattt ctttgtgtac aagggagagc tgccatatag tgccaagaag gtgctgagcg tgcacaaggc aaaagaaaaa tccaggcttg caaagagctc aaacacagac tcttgtcctc aggaggccgg tggaaaaaaa gaggggacag ggggctcggc agtctcagag acacctacaa agaggagacg tggtaagatc ccaaagagcg acaccaaaat ctgtgactgc cttgctggcc tcatcaagtc cgaggtcgac aggctagagc tgtcgattca gtccgaaagc accgaggtgg aacgctatgt acatctgatg atccttgaaa catttcagca ggaaagggga attgagttct ggcatattgc aggtcctaaa gccaccaaga ggccatcgga tgaagaaatg gcgagatgcc taagagagaa ggagtcaaga atggatgttc tattcgatgc aatcgctagg gagatccgga actatgacct ttactcagat gacctctctc tgttcaaagg cttccagaaa gaagacttcc tatttaacgc atgcgagtac tttctacttt ttattaagat ttcgtttctc agccagaaag atatgtcgtt taatagagca aggaaagagg cctatggcaa cattcttgct ctttttcccg acacatgcag aaactcaacg caggcccagt actaccttgc aaagcacatc tcgcaaacct gtggaatcag aggccttgga gcgcccagca gtgggaggca tgagggggag aatgcagagg acgatgtgtc ctcaaacttg gaaagaatgg aataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for EncuA.17526.a.A1.PS01162
PURIFICATION DATe: 7/21/2011
CONCENTRATION: 14.52mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 50µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Protocol Notes
notes unavailable
Validated AA Sequence
MVYPLSCRMD LEKEVTIQVN LMEPKEIDLR KKTKQKKRRQ RTDEYDYNDP FIEPFEGETE MVMIECNLED FFVYKGELPY SAKKVLSVHK AKEKSRLAKS SNTDSCPQEA GGKKEGTGGS AVSETPTKRR RGKIPKSDTK ICDCLAGLIK SEVDRLELSI QSESTEVERY VHLMILETFQ QERGIEFWHI AGPKATKRPS DEEMARCLRE KESRMDVLFD AIAREIRNYD LYSDDLSLFK GFQKEDFLFN ACEYFLLFIK ISFLSQKDMS FNRARKEAYG NILALFPDTC RNSTQAQYYL AKHISQTCGI RGLGAPSSGR HEGENAEDDV SSNLERM
Validated NT Sequence
ttccattctt tccaagtttg aggacacatc gtcctctgca ttctccccct catgcctccc actgctgggc gctccaaggc ctctgattcc acaggtttgc gagatgtgct ttgcaaggta gtactgggcc tgcgttgagt ttctgcatgt gtcgggaaaa agagcaagaa tgttgccata ggcctctttc cttgctctat taaacgacat atctttctgg ctgagaaacg aaatcttaat aaaaagtaga aagtactcgc atgcgttaaa taggaagtct tctttctgga agcctttgaa cagagagagg tcatctgagt aaaggtcata gttccggatc tccctagcga ttgcatcgaa tagaacatcc attcttgact ccttctctct taggcatctc gccatttctt catccgatgg cctcttggtg gctttaggac ctgcaatatg ccagaactca attccccttt cctgctgaaa tgtttcaagg atcatcagat gtacatagcg ttccacctcg gtgctttcgg actgaatcga cagctctagc ctgtcgacct cggacttgat gaggccagca aggcagtcac agattttggt gtcgctcttt gggatcttac cacgtctcct ctttgtaggt gtctctgaga ctgccgagcc ccctgtcccc tctttttttc caccggcctc ctgaggacaa gagtctgtgt ttgagctctt tgcaagcctg gatttttctt ttgccttgtg cacgctcagc accttcttgg cactatatgg cagctctccc ttgtacacaa agaaatcctc gaggttgcat tctatcatca ccatctccgt ctctccctca aagggctcta tgaacgggtc gttgtagtca tactcgtccg tcctttgcct ccttttcttt tgttttgtct tcttcctaag atcgatctcc ttcggctcca tgagattcac ctggattgta acctcttttt caagatccat tctgcaagaa aggggataga ccatcgaacc aggaccctgg gtctgagctt ccagggtacc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVYPLSCRM DLEKEVTIQV NLMEPKEIDL RKKTKQKKRR QRTDEYDYND PFIEPFEGET EMVMIECNLE DFFVYKGELP YSAKKVLSVH KAKEKSRLAK SSNTDSCPQE AGGKKEGTGG SAVSETPTKR RRGKIPKSDT KICDCLAGLI KSEVDRLELS IQSESTEVER YVHLMILETF QQERGIEFWH IAGPKATKRP SDEEMARCLR EKESRMDVLF DAIAREIRNY DLYSDDLSLF KGFQKEDFLF NACEYFLLFI KISFLSQKDM SFNRARKEAY GNILALFPDT CRNSTQAQYY LAKHISQTCG IRGLGAPSSG RHEGENAEDD VSSNLERME
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTCTATCCC CTTTCTTGCA GAATGGATCT TGAAAAAGAG GTTACAATCC AGGTGAATCT CATGGAGCCG AAGGAGATCG ATCTTAGGAA GAAGACAAAA CAAAAGAAAA GGAGGCAAAG GACGGACGAG TATGACTACA ACGACCCGTT CATAGAGCCC TTTGAGGGAG AGACGGAGAT GGTGATGATA GAATGCAACC TCGAGGATTT CTTTGTGTAC AAGGGAGAGC TGCCATATAG TGCCAAGAAG GTGCTGAGCG TGCACAAGGC AAAAGAAAAA TCCAGGCTTG CAAAGAGCTC AAACACAGAC TCTTGTCCTC AGGAGGCCGG TGGAAAAAAA GAGGGGACAG GGGGCTCGGC AGTCTCAGAG ACACCTACAA AGAGGAGACG TGGTAAGATC CCAAAGAGCG ACACCAAAAT CTGTGACTGC CTTGCTGGCC TCATCAAGTC CGAGGTCGAC AGGCTAGAGC TGTCGATTCA GTCCGAAAGC ACCGAGGTGG AACGCTATGT ACATCTGATG ATCCTTGAAA CATTTCAGCA GGAAAGGGGA ATTGAGTTCT GGCATATTGC AGGTCCTAAA GCCACCAAGA GGCCATCGGA TGAAGAAATG GCGAGATGCC TAAGAGAGAA GGAGTCAAGA ATGGATGTTC TATTCGATGC AATCGCTAGG GAGATCCGGA ACTATGACCT TTACTCAGAT GACCTCTCTC TGTTCAAAGG CTTCCAGAAA GAAGACTTCC TATTTAACGC ATGCGAGTAC TTTCTACTTT TTATTAAGAT TTCGTTTCTC AGCCAGAAAG ATATGTCGTT TAATAGAGCA AGGAAAGAGG CCTATGGCAA CATTCTTGCT CTTTTTCCCG ACACATGCAG AAACTCAACG CAGGCCCAGT ACTACCTTGC AAAGCACATC TCGCAAACCT GTGGAATCAG AGGCCTTGGA GCGCCCAGCA GTGGGAGGCA TGAGGGGGAG AATGCAGAGG ACGATGTGTC CTCAAACTTG GAAAGAATGG AATAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA