EncuA.17487.a

Putative uncharacterized protein ECU02_1170

CENTER ID: EncuA.17487.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.17487.a.A1.GE30869 Full length( EncuA.17487.a ) 1 219
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.17487.a.A1.PS00984 Full length( EncuA.17487.a ) 1 219

External Resources

RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU02_1170
RefSeq: NP_584642.1
UniProt: Q8SWA9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAFQKDANFC SPMTSIPKSN ELVFVKNYYN MLCNRPSVIH QYYMKDSQLT ISKETDKPET CTEDFGKYIK LKITNPVSKV LISHISWQRM DDLKSVINVI GQFVYSDLSQ TRIAHQFIVI KVNSVLYIKN EILTFLDEEV VYETNQDAKK AVTIEYGKNS MLQAIGVVSE FGKVESMKTG DEGRIVVTFG TVEDVNNIKK NIAKIISQGY KLEFDGLKI
NT Sequence
ATGGCCTTCC AAAAAGATGC AAATTTTTGC TCCCCGATGA CAAGTATTCC CAAGTCGAAC GAGCTGGTCT TCGTAAAGAA CTACTACAAC ATGCTCTGCA ACAGGCCATC GGTTATCCAC CAATACTACA TGAAAGATTC CCAGCTCACA ATAAGCAAGG AGACGGACAA ACCGGAGACA TGCACTGAGG ACTTTGGAAA GTATATCAAA TTGAAGATAA CCAATCCGGT ATCGAAAGTA CTTATATCAC ATATATCCTG GCAGAGGATG GACGACCTGA AGTCGGTCAT CAATGTCATT GGGCAGTTTG TTTACAGCGA TCTGTCGCAG ACAAGAATAG CTCATCAGTT TATTGTGATA AAGGTCAATT CTGTGCTATA CATCAAGAAC GAAATTCTGA CGTTTCTGGA CGAAGAAGTT GTCTATGAGA CGAATCAGGA TGCCAAGAAG GCTGTAACTA TTGAATATGG GAAAAACAGC ATGTTACAGG CAATCGGCGT TGTCTCCGAG TTTGGAAAAG TAGAGTCTAT GAAGACTGGA GATGAAGGAA GGATCGTTGT GACATTTGGC ACTGTAGAAG ACGTGAATAA CATCAAGAAA AACATTGCAA AGATCATCTC GCAGGGATAC AAATTAGAGT TTGATGGGCT GAAAATA
Details for EncuA.17487.a.A1.GE30869
HARVESTED ON: 11/16/2010
SEQUENCED ON: 11/23/2010
EXPECTED MW: 27kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAFQKDANF CSPMTSIPKS NELVFVKNYY NMLCNRPSVI HQYYMKDSQL TISKETDKPE TCTEDFGKYI KLKITNPVSK VLISHISWQR MDDLKSVINV IGQFVYSDLS QTRIAHQFIV IKVNSVLYIK NEILTFLDEE VVYETNQDAK KAVTIEYGKN SMLQAIGVVS EFGKVESMKT GDEGRIVVTF GTVEDVNNIK KNIAKIISQG YKLEFDGL
Validated NT Sequence
tcagcccatc aaactctaat ttgtatccct gcgagatgat ctttgcaatg tttttcttga tgttattcac gtcttctaca gtgccaaatg tcacaacgat ccttccttca tctccagtct tcatagactc tacttttcca aactcggaga caacgccgat tgcctgtaac atgctgtttt tcccatattc aatagttaca gccttcttgg catcctgatt cgtctcatag acaacttctt cgtccagaaa cgtcagaatt tcgttcttga tgtatagcac agaattgacc tttatcacaa taaactgatg agctattctt gtctgcgaca gatcgctgta aacaaactgc ccaatgacat tgatgaccga cttcaggtcg tccatcctct gccaggatat atgtgatata agtactttcg ataccggatt ggttatcttc aatttgatat actttccaaa gtcctcagtg catgtctccg gtttgtccgt ctccttgctt attgtgagct gggaatcttt catgtagtat tggtggataa ccgatggcct gttgcagagc atgttgtagt agttctttac gaagaccagc tcgttcgact tgggaatact tgtcatcggg gagcaaaaat ttgcatcttt ttggaaggcc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAFQKDANF CSPMTSIPKS NELVFVKNYY NMLCNRPSVI HQYYMKDSQL TISKETDKPE TCTEDFGKYI KLKITNPVSK VLISHISWQR MDDLKSVINV IGQFVYSDLS QTRIAHQFIV IKVNSVLYIK NEILTFLDEE VVYETNQDAK KAVTIEYGKN SMLQAIGVVS EFGKVESMKT GDEGRIVVTF GTVEDVNNIK KNIAKIISQG YKLEFDGLKI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccttccaa aaagatgcaa atttttgctc cccgatgaca agtattccca agtcgaacga gctggtcttc gtaaagaact actacaacat gctctgcaac aggccatcgg ttatccacca atactacatg aaagattccc agctcacaat aagcaaggag acggacaaac cggagacatg cactgaggac tttggaaagt atatcaaatt gaagataacc aatccggtat cgaaagtact tatatcacat atatcctggc agaggatgga cgacctgaag tcggtcatca atgtcattgg gcagtttgtt tacagcgatc tgtcgcagac aagaatagct catcagttta ttgtgataaa ggtcaattct gtgctataca tcaagaacga aattctgacg tttctggacg aagaagttgt ctatgagacg aatcaggatg ccaagaaggc tgtaactatt gaatatggga aaaacagcat gttacaggca atcggcgttg tctccgagtt tggaaaagta gagtctatga agactggaga tgaaggaagg atcgttgtga catttggcac tgtagaagac gtgaataaca tcaagaaaaa cattgcaaag atcatctcgc agggatacaa attagagttt gatgggctga aaatataaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for EncuA.17487.a.A1.PS00984
PURIFICATION DATe: 2/27/2011
CONCENTRATION: 35.17mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 13
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAFQKDANF CSPMTSIPKS NELVFVKNYY NMLCNRPSVI HQYYMKDSQL TISKETDKPE TCTEDFGKYI KLKITNPVSK VLISHISWQR MDDLKSVINV IGQFVYSDLS QTRIAHQFIV IKVNSVLYIK NEILTFLDEE VVYETNQDAK KAVTIEYGKN SMLQAIGVVS EFGKVESMKT GDEGRIVVTF GTVEDVNNIK KNIAKIISQG YKLEFDGL
Validated NT Sequence
tcagcccatc aaactctaat ttgtatccct gcgagatgat ctttgcaatg tttttcttga tgttattcac gtcttctaca gtgccaaatg tcacaacgat ccttccttca tctccagtct tcatagactc tacttttcca aactcggaga caacgccgat tgcctgtaac atgctgtttt tcccatattc aatagttaca gccttcttgg catcctgatt cgtctcatag acaacttctt cgtccagaaa cgtcagaatt tcgttcttga tgtatagcac agaattgacc tttatcacaa taaactgatg agctattctt gtctgcgaca gatcgctgta aacaaactgc ccaatgacat tgatgaccga cttcaggtcg tccatcctct gccaggatat atgtgatata agtactttcg ataccggatt ggttatcttc aatttgatat actttccaaa gtcctcagtg catgtctccg gtttgtccgt ctccttgctt attgtgagct gggaatcttt catgtagtat tggtggataa ccgatggcct gttgcagagc atgttgtagt agttctttac gaagaccagc tcgttcgact tgggaatact tgtcatcggg gagcaaaaat ttgcatcttt ttggaaggcc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAFQKDANF CSPMTSIPKS NELVFVKNYY NMLCNRPSVI HQYYMKDSQL TISKETDKPE TCTEDFGKYI KLKITNPVSK VLISHISWQR MDDLKSVINV IGQFVYSDLS QTRIAHQFIV IKVNSVLYIK NEILTFLDEE VVYETNQDAK KAVTIEYGKN SMLQAIGVVS EFGKVESMKT GDEGRIVVTF GTVEDVNNIK KNIAKIISQG YKLEFDGLKI
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCCTTCCAA AAAGATGCAA ATTTTTGCTC CCCGATGACA AGTATTCCCA AGTCGAACGA GCTGGTCTTC GTAAAGAACT ACTACAACAT GCTCTGCAAC AGGCCATCGG TTATCCACCA ATACTACATG AAAGATTCCC AGCTCACAAT AAGCAAGGAG ACGGACAAAC CGGAGACATG CACTGAGGAC TTTGGAAAGT ATATCAAATT GAAGATAACC AATCCGGTAT CGAAAGTACT TATATCACAT ATATCCTGGC AGAGGATGGA CGACCTGAAG TCGGTCATCA ATGTCATTGG GCAGTTTGTT TACAGCGATC TGTCGCAGAC AAGAATAGCT CATCAGTTTA TTGTGATAAA GGTCAATTCT GTGCTATACA TCAAGAACGA AATTCTGACG TTTCTGGACG AAGAAGTTGT CTATGAGACG AATCAGGATG CCAAGAAGGC TGTAACTATT GAATATGGGA AAAACAGCAT GTTACAGGCA ATCGGCGTTG TCTCCGAGTT TGGAAAAGTA GAGTCTATGA AGACTGGAGA TGAAGGAAGG ATCGTTGTGA CATTTGGCAC TGTAGAAGAC GTGAATAACA TCAAGAAAAA CATTGCAAAG ATCATCTCGC AGGGATACAA ATTAGAGTTT GATGGGCTGA AAATATAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA