SSGCID: Target Page

EncuA.17477.a

RNA 3'TERMINAL PHOSPHATE CYCLASE

CENTER ID:	EncuA.17477.a
ORGANISM:	Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE:	Microsporidiosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EncuA.17477.a.A1.GE30868	Full length( EncuA.17477.a )		1	341

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EncuA.17477.a.A1.PW30756	Full length( EncuA.17477.a )		1	341

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	MicrosporidiaDB:ECU06_1030
RefSeq:	NP_585859.1
UniProt:	Q8SRP7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNGGSEMVFE CPESLELILV CSLLSKNTVK VAVGRRYSMA KSFVKLLQGI SHGSSHSWEE GYVLFAPGAL RGGDTTVVCS DEISMYIEPL LVLCPFVTEP LRIRFKGITN GKLCVDLIRI AHFNVLRRFG INGCEIVVRK RGFAPEGKGE VLFTVDRPGK VCTIALESPE KILKVRGLVL SSRVSSVPAK EMTDRIKEML GDILNTKVFS NMSNRVDSGP SPGFQCAVFA ESKSGIYYSI GSGEGLTPRE TATAACKDLL RSVKYGGVFD KKLLYLALSL MSLSSTSIGS LWVGRVDSEV QGILDLLKRF FGFEYEVRST GEGHVVYGPG CGLTNVNRLL R

NT Sequence

ATGAATGGTG GAAGCGAGAT GGTCTTCGAG TGTCCGGAGA GCCTAGAGCT TATTCTTGTA TGCAGTCTAC TGTCCAAGAA CACGGTGAAG GTGGCGGTTG GCAGGAGATA CTCAATGGCG AAGTCATTTG TCAAGCTCCT CCAGGGTATA TCCCACGGGT CGAGCCACTC GTGGGAGGAG GGATATGTTC TTTTTGCTCC TGGTGCGCTG AGGGGAGGAG ATACTACTGT CGTGTGCTCG GATGAGATTT CGATGTACAT AGAGCCTCTT CTGGTTCTCT GTCCCTTTGT GACTGAGCCC TTAAGGATAA GATTCAAGGG GATAACGAAC GGGAAGCTTT GCGTTGATCT GATTAGAATT GCCCATTTCA ATGTGTTGAG GAGATTTGGA ATCAACGGGT GTGAGATTGT TGTAAGGAAG AGGGGGTTTG CGCCTGAGGG CAAGGGGGAG GTTCTATTCA CTGTGGATAG GCCTGGAAAG GTATGTACGA TTGCCCTAGA GAGCCCGGAG AAGATTCTCA AGGTCCGAGG GCTGGTGCTG AGCAGCAGGG TAAGCTCCGT TCCGGCTAAA GAAATGACCG ACCGGATCAA GGAGATGCTG GGAGATATCT TGAACACAAA GGTTTTCTCG AACATGTCGA ACAGGGTAGA CTCTGGGCCA TCTCCTGGAT TCCAGTGTGC TGTTTTTGCA GAGTCCAAGA GCGGGATTTA TTATTCGATC GGGAGTGGGG AGGGCCTGAC GCCAAGAGAA ACGGCCACGG CGGCGTGCAA GGACCTTCTT CGAAGCGTTA AGTACGGAGG GGTATTTGAC AAGAAGCTTC TCTATCTTGC ACTCTCTCTG ATGTCGCTGT CGTCCACAAG CATAGGGAGT CTGTGGGTTG GCAGGGTTGA TTCGGAGGTT CAGGGGATTC TGGATTTGCT AAAGCGGTTC TTTGGGTTTG AATATGAAGT TCGAAGTACT GGAGAAGGAC ATGTTGTCTA TGGACCTGGA TGTGGACTTA CCAATGTCAA CAGGCTGTTG AGA

Details for EncuA.17477.a.A1.GE30868

HARVESTED ON:	11/16/2010
SEQUENCED ON:	11/23/2010
EXPECTED MW:	39kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Validated AA Sequence

Validated NT Sequence

ctcaacagcc tgttgacatt ggtaagtcca catccaggtc catagacaac atgtccttct ccagtacttc gaacttcata ttcaaaccca aagaaccgct ttagcaaatc cagaatcccc tgaacctccg aatcaaccct gccaacccac agactcccta tgcttgtgga cgacagcgac atcagagaga gtgcaagata gagaagcttc ttgtcaaata cccctccgta cttaacgctt cgaagaaggt ccttgcacgc cgccgtggcc gtttctcttg gcgtcaggcc ctccccactc ccgatcgaat aataaatccc gctcttggac tctgcaaaaa cagcacactg gaatccagga gatggcccag agtctaccct gttcgacatg ttcgagaaaa cctttgtgtt caagatatct cccagcatct ccttgatccg gtcggtcatt tctttagccg gaacggagct taccctgctg ctcagcacca gccctcggac cttgagaatc ttctccgggc tctctagggc aatcgtacat acctttccag gcctatccac agtgaataga acctccccct tgccctcagg cgcaaacccc ctcttcctta caacaatctc acacccgttg attccaaatc tcctcaacac attgaaatgg gcaattctaa tcagatcaac gcaaagcttc ccgttcgtta tccccttgaa tcttatcctt aagggctcag tcacaaaggg acagagaacc agaagaggct ctatgtacat cgaaatctca tccgagcaca cgacagtagt atctcctccc ctcagcgcac caggagcaaa aagaacatat ccctcctccc acgagtggct cgacccgtgg gatataccct ggaggagctt gacaaatgac ttcgccattg agtatctcct gccaaccgcc accttcaccg tgttcttgga cagtagactg catacaagaa taagctctag gctctccgga cactcgaaga ccatctcgct tccaccattc atcgaaccag gaccctgggt ctgagcttcc ag

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMNGGSEMVF ECPESLELIL VCSLLSKNTV KVAVGRRYSM AKSFVKLLQG ISHGSSHSWE EGYVLFAPGA LRGGDTTVVC SDEISMYIEP LLVLCPFVTE PLRIRFKGIT NGKLCVDLIR IAHFNVLRRF GINGCEIVVR KRGFAPEGKG EVLFTVDRPG KVCTIALESP EKILKVRGLV LSSRVSSVPA KEMTDRIKEM LGDILNTKVF SNMSNRVDSG PSPGFQCAVF AESKSGIYYS IGSGEGLTPR ETATAACKDL LRSVKYGGVF DKKLLYLALS LMSLSSTSIG SLWVGRVDSE VQGILDLLKR FFGFEYEVRS TGEGHVVYGP GCGLTNVNRL LR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaatggtgga agcgagatgg tcttcgagtg tccggagagc ctagagctta ttcttgtatg cagtctactg tccaagaaca cggtgaaggt ggcggttggc aggagatact caatggcgaa gtcatttgtc aagctcctcc agggtatatc ccacgggtcg agccactcgt gggaggaggg atatgttctt tttgctcctg gtgcgctgag gggaggagat actactgtcg tgtgctcgga tgagatttcg atgtacatag agcctcttct ggttctctgt ccctttgtga ctgagccctt aaggataaga ttcaagggga taacgaacgg gaagctttgc gttgatctga ttagaattgc ccatttcaat gtgttgagga gatttggaat caacgggtgt gagattgttg taaggaagag ggggtttgcg cctgagggca agggggaggt tctattcact gtggataggc ctggaaaggt atgtacgatt gccctagaga gcccggagaa gattctcaag gtccgagggc tggtgctgag cagcagggta agctccgttc cggctaaaga aatgaccgac cggatcaagg agatgctggg agatatcttg aacacaaagg ttttctcgaa catgtcgaac agggtagact ctgggccatc tcctggattc cagtgtgctg tttttgcaga gtccaagagc gggatttatt attcgatcgg gagtggggag ggcctgacgc caagagaaac ggccacggcg gcgtgcaagg accttcttcg aagcgttaag tacggagggg tatttgacaa gaagcttctc tatcttgcac tctctctgat gtcgctgtcg tccacaagca tagggagtct gtgggttggc agggttgatt cggaggttca ggggattctg gatttgctaa agcggttctt tgggtttgaa tatgaagttc gaagtactgg agaaggacat gttgtctatg gacctggatg tggacttacc aatgtcaaca ggctgttgag ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa

Details for EncuA.17477.a.A1.PW30756

PURIFICATION DATe:	3/25/2011
CONCENTRATION:	40.55mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAATGGTGGA AGCGAGATGG TCTTCGAGTG TCCGGAGAGC CTAGAGCTTA TTCTTGTATG CAGTCTACTG TCCAAGAACA CGGTGAAGGT GGCGGTTGGC AGGAGATACT CAATGGCGAA GTCATTTGTC AAGCTCCTCC AGGGTATATC CCACGGGTCG AGCCACTCGT GGGAGGAGGG ATATGTTCTT TTTGCTCCTG GTGCGCTGAG GGGAGGAGAT ACTACTGTCG TGTGCTCGGA TGAGATTTCG ATGTACATAG AGCCTCTTCT GGTTCTCTGT CCCTTTGTGA CTGAGCCCTT AAGGATAAGA TTCAAGGGGA TAACGAACGG GAAGCTTTGC GTTGATCTGA TTAGAATTGC CCATTTCAAT GTGTTGAGGA GATTTGGAAT CAACGGGTGT GAGATTGTTG TAAGGAAGAG GGGGTTTGCG CCTGAGGGCA AGGGGGAGGT TCTATTCACT GTGGATAGGC CTGGAAAGGT ATGTACGATT GCCCTAGAGA GCCCGGAGAA GATTCTCAAG GTCCGAGGGC TGGTGCTGAG CAGCAGGGTA AGCTCCGTTC CGGCTAAAGA AATGACCGAC CGGATCAAGG AGATGCTGGG AGATATCTTG AACACAAAGG TTTTCTCGAA CATGTCGAAC AGGGTAGACT CTGGGCCATC TCCTGGATTC CAGTGTGCTG TTTTTGCAGA GTCCAAGAGC GGGATTTATT ATTCGATCGG GAGTGGGGAG GGCCTGACGC CAAGAGAAAC GGCCACGGCG GCGTGCAAGG ACCTTCTTCG AAGCGTTAAG TACGGAGGGG TATTTGACAA GAAGCTTCTC TATCTTGCAC TCTCTCTGAT GTCGCTGTCG TCCACAAGCA TAGGGAGTCT GTGGGTTGGC AGGGTTGATT CGGAGGTTCA GGGGATTCTG GATTTGCTAA AGCGGTTCTT TGGGTTTGAA TATGAAGTTC GAAGTACTGG AGAAGGACAT GTTGTCTATG GACCTGGATG TGGACTTACC AATGTCAACA GGCTGTTGAG ATAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA