EncuA.17366.a

Mitochondrial protein import protein mas5

CENTER ID: EncuA.17366.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.17366.a.A1.GE30867 Full length( EncuA.17366.a ) 1 398
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.17366.a.A1.PS00990 Full length( EncuA.17366.a ) 1 398

External Resources

RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU07_0760
RefSeq: NP_586004.1
UniProt: Q8SRK0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSKDPKGYYK VLELSPGASV AEVRKAYAKQ QAKYHLDSPY MKNKLKNAAS DEEREKIKKE CGEMSARLNS AKSVLFDEKK KKEYDSGMGE FGAHFSGGGY SDIFDIFSQF TGGRGHQRTN KVSSTKYVIT VSLRESFVGK VSKFNVRTEK VCTTCDGKGG KDVETCKKCN GNGVYTSRRS LGGFVTLAET RCDGCDGSGH KIKGKPCSTC NGAEYIQDKT MFEVNIKPGV RKGEKIVFEG MGDQRRGHVP GDVIFIIDVQ EDSRFERCGN DLVGNIDIPL YTAIGGGVVY FTHIDGRQLE INVSPFRTFD TALKIRNEGF KGSRTGNLIL KPNIIIGSES DRAKIMQVLS APSKKPYGTF TKVNSEFGSM PEPERDHEDA SEEGAQSARS FFNNFSFF
NT Sequence
ATGTCCAAAG ATCCAAAGGG ATATTATAAA GTGCTTGAGC TGAGTCCTGG GGCGTCTGTA GCGGAGGTAA GAAAAGCCTA TGCGAAGCAG CAGGCGAAGT ATCACCTGGA CTCGCCGTAC ATGAAGAACA AGCTGAAGAA TGCAGCCAGT GACGAGGAGA GGGAGAAGAT AAAGAAGGAG TGTGGAGAGA TGTCTGCAAG GCTGAACTCT GCCAAGAGTG TGCTGTTTGA TGAGAAGAAG AAGAAGGAGT ATGACAGCGG GATGGGGGAG TTTGGAGCAC ACTTCTCTGG AGGAGGGTAC TCTGACATAT TTGACATATT CAGCCAGTTT ACTGGTGGCA GGGGGCACCA GAGAACAAAC AAGGTCAGCT CGACGAAGTA TGTCATCACA GTGTCTCTCA GAGAGTCGTT TGTTGGGAAG GTATCGAAGT TTAATGTCAG GACCGAGAAG GTATGTACGA CCTGTGATGG AAAGGGAGGC AAGGATGTGG AGACATGCAA GAAGTGCAAC GGAAATGGGG TGTATACCAG TCGCAGGAGC CTTGGAGGCT TTGTGACGCT CGCAGAAACA CGATGTGACG GGTGTGACGG CTCTGGGCAC AAGATCAAGG GCAAGCCCTG CAGTACGTGT AATGGCGCTG AGTACATTCA GGACAAGACG ATGTTTGAGG TGAATATAAA GCCGGGTGTC AGGAAGGGAG AGAAGATTGT GTTTGAGGGC ATGGGAGACC AGAGAAGAGG CCATGTTCCT GGCGACGTGA TCTTTATAAT TGATGTGCAG GAAGACAGCA GATTTGAGAG ATGTGGAAAC GACCTTGTAG GAAATATAGA CATTCCTCTG TACACCGCGA TTGGGGGAGG GGTGGTTTAC TTCACCCACA TTGATGGAAG GCAGCTTGAG ATAAATGTGA GTCCGTTCAG GACGTTCGAC ACTGCGCTCA AGATTCGAAA CGAGGGATTC AAGGGGAGCA GGACTGGAAA TCTAATTCTC AAGCCGAACA TCATAATTGG AAGCGAGTCT GACAGGGCAA AGATTATGCA GGTGCTGAGT GCGCCTTCCA AGAAGCCCTA CGGGACATTT ACAAAGGTGA ACAGCGAGTT TGGAAGCATG CCTGAGCCTG AGAGGGATCA TGAGGATGCC TCTGAGGAGG GAGCACAGAG TGCCAGGAGC TTCTTCAACA ACTTCAGCTT CTTC
Details for EncuA.17366.a.A1.GE30867
HARVESTED ON: 11/16/2010
SEQUENCED ON: 11/23/2010
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MSKDPKGYYK VLELSPGASV AEVRKAYAKQ QAKYHLDSPY MKNKLKNAAS DEEREKIKKE CGEMSARLNS AKSVLFDEKK KKEYDSGMGE FGAHFSGGGY SDIFDIFSQF TGGRGHQRTN KVSSTKYVIT VSLRESFVGK VSKFNVRTEK VCTTCDGKGG KDVETCKKCN GNGVYTSRRS LGGFVTLAET RCDGCDGSGH KIKGKPCSTC NGAEYIQDKT MFEVNIKPGV RKGEKIVFEG MGDQRRGHVP GDVIFIIDVQ EDSRFERCGN DLVGNIDIPL YTAIGGGVVY FTHIDGRQLE INVSPFRTFD TALKIRNEGF KGSRTGNLIL KPNIIIGSES DRAKIMQVLS APSKKPYGTF TKVNSEFGSM PEPERDHEDA SEEGAQSARS FFNNFSFF
Validated NT Sequence
tagaagaagc tgaagttgtt gaagaagctc ctggcactct gtgctccctc ctcagaggca tcctcatgat ccctctcagg ctcaggcatg cttccaaact cgctgttcac ctttgtaaat gtcccgtagg gcttcttgga aggcgcactc agcacctgca taatctttgc cctgtcagac tcgcttccaa ttatgatgtt cggcttgaga attagatttc cagtcctgct ccccttgaat ccctcgtttc gaatcttgag cgcagtgtcg aacgtcctga acggactcac atttatctca agctgccttc catcaatgtg ggtgaagtaa accacccctc ccccaatcgc ggtgtacaga ggaatgtcta tatttcctac aaggtcgttt ccacatctct caaatctgct gtcttcctgc acatcaatta taaagatcac gtcgccagga acatggcctc ttctctggtc tcccatgccc tcaaacacaa tcttctctcc cttcctgaca cccggcttta tattcacctc aaacatcgtc ttgtcctgaa tgtactcagc gccattacac gtactgcagg gcttgccctt gatcttgtgc ccagagccgt cacacccgtc acatcgtgtt tctgcgagcg tcacaaagcc tccaaggctc ctgcgactgg tatacacccc atttccgttg cacttcttgc atgtctccac atccttgcct ccctttccat cacaggtcgt acataccttc tcggtcctga cattaaactt cgataccttc ccaacaaacg actctctgag agacactgtg atgacatact tcgtcgagct gaccttgttt gttctctggt gccccctgcc accagtaaac tggctgaata tgtcaaatat gtcagagtac cctcctccag agaagtgtgc tccaaactcc cccatcccgc tgtcatactc cttcttcttc ttctcatcaa acagcacact cttggcagag ttcagccttg cagacatctc tccacactcc ttctttatct tctccctctc ctcgtcactg gctgcattct tcagcttgtt cttcatgtac ggcgagtcca ggtgatactt cgcctgctgc ttcgcatagg cttttcttac ctccgctaca gacgccccag gactcagctc aagcacttta taatatccct ttggatcttt ggacatcgaa ccaggaccct gggtctgagc ttccagggt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSKDPKGYY KVLELSPGAS VAEVRKAYAK QQAKYHLDSP YMKNKLKNAA SDEEREKIKK ECGEMSARLN SAKSVLFDEK KKKEYDSGMG EFGAHFSGGG YSDIFDIFSQ FTGGRGHQRT NKVSSTKYVI TVSLRESFVG KVSKFNVRTE KVCTTCDGKG GKDVETCKKC NGNGVYTSRR SLGGFVTLAE TRCDGCDGSG HKIKGKPCST CNGAEYIQDK TMFEVNIKPG VRKGEKIVFE GMGDQRRGHV PGDVIFIIDV QEDSRFERCG NDLVGNIDIP LYTAIGGGVV YFTHIDGRQL EINVSPFRTF DTALKIRNEG FKGSRTGNLI LKPNIIIGSE SDRAKIMQVL SAPSKKPYGT FTKVNSEFGS MPEPERDHED ASEEGAQSAR SFFNNFSFF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtccaaagat ccaaagggat attataaagt gcttgagctg agtcctgggg cgtctgtagc ggaggtaaga aaagcctatg cgaagcagca ggcgaagtat cacctggact cgccgtacat gaagaacaag ctgaagaatg cagccagtga cgaggagagg gagaagataa agaaggagtg tggagagatg tctgcaaggc tgaactctgc caagagtgtg ctgtttgatg agaagaagaa gaaggagtat gacagcggga tgggggagtt tggagcacac ttctctggag gagggtactc tgacatattt gacatattca gccagtttac tggtggcagg gggcaccaga gaacaaacaa ggtcagctcg acgaagtatg tcatcacagt gtctctcaga gagtcgtttg ttgggaaggt atcgaagttt aatgtcagga ccgagaaggt atgtacgacc tgtgatggaa agggaggcaa ggatgtggag acatgcaaga agtgcaacgg aaatggggtg tataccagtc gcaggagcct tggaggcttt gtgacgctcg cagaaacacg atgtgacggg tgtgacggct ctgggcacaa gatcaagggc aagccctgca gtacgtgtaa tggcgctgag tacattcagg acaagacgat gtttgaggtg aatataaagc cgggtgtcag gaagggagag aagattgtgt ttgagggcat gggagaccag agaagaggcc atgttcctgg cgacgtgatc tttataattg atgtgcagga agacagcaga tttgagagat gtggaaacga ccttgtagga aatatagaca ttcctctgta caccgcgatt gggggagggg tggtttactt cacccacatt gatggaaggc agcttgagat aaatgtgagt ccgttcagga cgttcgacac tgcgctcaag attcgaaacg agggattcaa ggggagcagg actggaaatc taattctcaa gccgaacatc ataattggaa gcgagtctga cagggcaaag attatgcagg tgctgagtgc gccttccaag aagccctacg ggacatttac aaaggtgaac agcgagtttg gaagcatgcc tgagcctgag agggatcatg aggatgcctc tgaggaggga gcacagagtg ccaggagctt cttcaacaac ttcagcttct tctaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for EncuA.17366.a.A1.PS00990
PURIFICATION DATe: 2/28/2011
CONCENTRATION: 29.6mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MSKDPKGYYK VLELSPGASV AEVRKAYAKQ QAKYHLDSPY MKNKLKNAAS DEEREKIKKE CGEMSARLNS AKSVLFDEKK KKEYDSGMGE FGAHFSGGGY SDIFDIFSQF TGGRGHQRTN KVSSTKYVIT VSLRESFVGK VSKFNVRTEK VCTTCDGKGG KDVETCKKCN GNGVYTSRRS LGGFVTLAET RCDGCDGSGH KIKGKPCSTC NGAEYIQDKT MFEVNIKPGV RKGEKIVFEG MGDQRRGHVP GDVIFIIDVQ EDSRFERCGN DLVGNIDIPL YTAIGGGVVY FTHIDGRQLE INVSPFRTFD TALKIRNEGF KGSRTGNLIL KPNIIIGSES DRAKIMQVLS APSKKPYGTF TKVNSEFGSM PEPERDHEDA SEEGAQSARS FFNNFSFF
Validated NT Sequence
tagaagaagc tgaagttgtt gaagaagctc ctggcactct gtgctccctc ctcagaggca tcctcatgat ccctctcagg ctcaggcatg cttccaaact cgctgttcac ctttgtaaat gtcccgtagg gcttcttgga aggcgcactc agcacctgca taatctttgc cctgtcagac tcgcttccaa ttatgatgtt cggcttgaga attagatttc cagtcctgct ccccttgaat ccctcgtttc gaatcttgag cgcagtgtcg aacgtcctga acggactcac atttatctca agctgccttc catcaatgtg ggtgaagtaa accacccctc ccccaatcgc ggtgtacaga ggaatgtcta tatttcctac aaggtcgttt ccacatctct caaatctgct gtcttcctgc acatcaatta taaagatcac gtcgccagga acatggcctc ttctctggtc tcccatgccc tcaaacacaa tcttctctcc cttcctgaca cccggcttta tattcacctc aaacatcgtc ttgtcctgaa tgtactcagc gccattacac gtactgcagg gcttgccctt gatcttgtgc ccagagccgt cacacccgtc acatcgtgtt tctgcgagcg tcacaaagcc tccaaggctc ctgcgactgg tatacacccc atttccgttg cacttcttgc atgtctccac atccttgcct ccctttccat cacaggtcgt acataccttc tcggtcctga cattaaactt cgataccttc ccaacaaacg actctctgag agacactgtg atgacatact tcgtcgagct gaccttgttt gttctctggt gccccctgcc accagtaaac tggctgaata tgtcaaatat gtcagagtac cctcctccag agaagtgtgc tccaaactcc cccatcccgc tgtcatactc cttcttcttc ttctcatcaa acagcacact cttggcagag ttcagccttg cagacatctc tccacactcc ttctttatct tctccctctc ctcgtcactg gctgcattct tcagcttgtt cttcatgtac ggcgagtcca ggtgatactt cgcctgctgc ttcgcatagg cttttcttac ctccgctaca gacgccccag gactcagctc aagcacttta taatatccct ttggatcttt ggacatcgaa ccaggaccct gggtctgagc ttccagggt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSKDPKGYY KVLELSPGAS VAEVRKAYAK QQAKYHLDSP YMKNKLKNAA SDEEREKIKK ECGEMSARLN SAKSVLFDEK KKKEYDSGMG EFGAHFSGGG YSDIFDIFSQ FTGGRGHQRT NKVSSTKYVI TVSLRESFVG KVSKFNVRTE KVCTTCDGKG GKDVETCKKC NGNGVYTSRR SLGGFVTLAE TRCDGCDGSG HKIKGKPCST CNGAEYIQDK TMFEVNIKPG VRKGEKIVFE GMGDQRRGHV PGDVIFIIDV QEDSRFERCG NDLVGNIDIP LYTAIGGGVV YFTHIDGRQL EINVSPFRTF DTALKIRNEG FKGSRTGNLI LKPNIIIGSE SDRAKIMQVL SAPSKKPYGT FTKVNSEFGS MPEPERDHED ASEEGAQSAR SFFNNFSFF
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCCAAAGAT CCAAAGGGAT ATTATAAAGT GCTTGAGCTG AGTCCTGGGG CGTCTGTAGC GGAGGTAAGA AAAGCCTATG CGAAGCAGCA GGCGAAGTAT CACCTGGACT CGCCGTACAT GAAGAACAAG CTGAAGAATG CAGCCAGTGA CGAGGAGAGG GAGAAGATAA AGAAGGAGTG TGGAGAGATG TCTGCAAGGC TGAACTCTGC CAAGAGTGTG CTGTTTGATG AGAAGAAGAA GAAGGAGTAT GACAGCGGGA TGGGGGAGTT TGGAGCACAC TTCTCTGGAG GAGGGTACTC TGACATATTT GACATATTCA GCCAGTTTAC TGGTGGCAGG GGGCACCAGA GAACAAACAA GGTCAGCTCG ACGAAGTATG TCATCACAGT GTCTCTCAGA GAGTCGTTTG TTGGGAAGGT ATCGAAGTTT AATGTCAGGA CCGAGAAGGT ATGTACGACC TGTGATGGAA AGGGAGGCAA GGATGTGGAG ACATGCAAGA AGTGCAACGG AAATGGGGTG TATACCAGTC GCAGGAGCCT TGGAGGCTTT GTGACGCTCG CAGAAACACG ATGTGACGGG TGTGACGGCT CTGGGCACAA GATCAAGGGC AAGCCCTGCA GTACGTGTAA TGGCGCTGAG TACATTCAGG ACAAGACGAT GTTTGAGGTG AATATAAAGC CGGGTGTCAG GAAGGGAGAG AAGATTGTGT TTGAGGGCAT GGGAGACCAG AGAAGAGGCC ATGTTCCTGG CGACGTGATC TTTATAATTG ATGTGCAGGA AGACAGCAGA TTTGAGAGAT GTGGAAACGA CCTTGTAGGA AATATAGACA TTCCTCTGTA CACCGCGATT GGGGGAGGGG TGGTTTACTT CACCCACATT GATGGAAGGC AGCTTGAGAT AAATGTGAGT CCGTTCAGGA CGTTCGACAC TGCGCTCAAG ATTCGAAACG AGGGATTCAA GGGGAGCAGG ACTGGAAATC TAATTCTCAA GCCGAACATC ATAATTGGAA GCGAGTCTGA CAGGGCAAAG ATTATGCAGG TGCTGAGTGC GCCTTCCAAG AAGCCCTACG GGACATTTAC AAAGGTGAAC AGCGAGTTTG GAAGCATGCC TGAGCCTGAG AGGGATCATG AGGATGCCTC TGAGGAGGGA GCACAGAGTG CCAGGAGCTT CTTCAACAAC TTCAGCTTCT TCTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA