EncuA.17329.b

Putative uncharacterized protein ECU09_0300

CENTER ID: EncuA.17329.b
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.17329.b.A1.GE30866 Full length( EncuA.17329.b ) 1 344
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU09_0300
RefSeq: XP_955582.1
UniProt: Q8STW6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLRDFPKKRK IFNIAEMKEI EETNARADEA IYELKASLQT CSQACSPQVT VQPISESEAT RPLKDILLQG GSSKDPSEGL YERLFLGSAT VQRRRTEAKG SPSTEGEKDF HEFRYELLST SQKIELSRFC WSAFRKRATN MLFAWVNCFQ KKNRMGRGRQ ISPVVFSVVK SLKKRAIRTK FIFRYEPDKL AAAKIANKIH ENTCVDIENI DVLVLANALK NYIREHLDGL VPIEAFEKIK TCIISNDRYT REALFSYMPF IFTDAERRLL TSLFELLKMI DKNSDATEMN IDSLLGLFSL VLHPQAAFTT LDDLEYVKII TRELYQLDFD AIPQGVVVHE LVFV
NT Sequence
ATGCTGAGAG ATTTTCCGAA GAAAAGAAAG ATCTTTAACA TAGCAGAGAT GAAGGAGATA GAGGAAACAA ATGCAAGGGC AGACGAGGCC ATCTATGAGT TGAAGGCCTC TCTTCAAACA TGTTCTCAGG CATGCAGCCC TCAGGTTACG GTGCAGCCCA TTTCCGAAAG CGAAGCCACC AGACCCCTGA AAGACATTCT TCTCCAGGGG GGAAGCTCTA AGGATCCCAG TGAAGGGTTG TACGAGAGAC TTTTTCTTGG CTCGGCAACG GTCCAGAGGA GGAGGACGGA AGCAAAAGGC TCTCCGTCTA CTGAGGGGGA AAAGGACTTC CATGAATTTA GATATGAGCT TCTATCAACG TCGCAGAAGA TAGAGCTTAG CAGATTTTGT TGGAGTGCAT TTAGGAAGAG GGCTACGAAC ATGCTCTTTG CCTGGGTGAA TTGCTTTCAG AAGAAGAATC GGATGGGGCG TGGTAGACAA ATTAGCCCTG TGGTGTTCAG TGTCGTCAAG TCGTTGAAAA AAAGGGCAAT CCGCACCAAG TTCATCTTCC GCTATGAACC AGACAAGCTG GCAGCGGCAA AGATAGCAAA CAAGATACAT GAGAACACAT GCGTGGATAT CGAGAACATA GATGTCCTTG TGCTGGCGAA TGCCCTCAAG AATTATATCA GAGAGCACCT TGACGGCCTA GTTCCCATAG AGGCCTTTGA GAAGATCAAG ACTTGCATCA TCTCGAATGA TAGGTATACA AGAGAAGCAT TGTTCTCGTA CATGCCGTTT ATATTTACAG ATGCAGAGAG ACGCTTGCTC ACCTCGCTCT TTGAGCTTCT GAAGATGATT GATAAGAACT CGGATGCGAC GGAGATGAAT ATCGACTCAC TTCTGGGGCT GTTCAGCCTA GTCCTACACC CACAGGCAGC GTTTACAACC CTTGATGACC TAGAATATGT CAAGATTATC ACGAGGGAGT TATACCAACT CGACTTTGAT GCCATACCCC AAGGCGTTGT TGTCCATGAG CTGGTCTTTG TG
Details for EncuA.17329.b.A1.GE30866
HARVESTED ON: 11/16/2010
SEQUENCED ON: 11/23/2010
EXPECTED MW: 41kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 86
PERCENT COVERAGE: 103
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLRDFPKKR KIFNIAEMKE IEETNARADE AIYELKASLQ TCSQACSPQV TVQPISESEA TRPLKDILLQ GGSSKDPSEG LYERLFLGSA TVQRRRTEAK GSPSTEGEKD FHEFRYELLS TSQKIELSRF CWSAFRKRAT NMLFAWVNCF QKKNRMGRGR QISPVVFSVV KSLKKRAIRT KFIFRYEPDK LAAAKIANKI HENTYVDIEN IDVLVLANXL KNYIREHLDG LVPIEAFEKI KTCIISNDRX TREALXSYMP FIFTDAERRX XTSLFELLKM IXXNSXAXXX NIDSLLGLXX LXLHPXAAXX XXXIXXVXXH XXXXXSTXXX PXXXXXXXXX XXXXXXXXXX XXXXXXXXX
Validated NT Sequence
tttgtttact ttaagaagga ganataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgctgaga gattttccga agaaaagaaa gatctttaac atagcagaga tgaaggagat agaggaaaca aatgcaaggg cagacgaggc catctatgag ttgaaggcct ctcttcaaac atgttctcag gcatgcagcc ctcaggttac ggtgcagccc atttccgaaa gcgaagccac cagacccctg aaagacattc ttctccaggg gggaagctct aaggatccca gtgaagggtt gtacgagaga ctttttcttg gctcggcaac ggtccagagg aggaggacgg aagcaaaagg ctctccgtct actgaggggg aaaaggactt ccatgaattt agatatgagc ttctatcaac gtcgcagaag atagagctta gcagattttg ttggagtgca tttaggaaga gggctacgaa catgctcttt gcctgggtga attgctttca gaagaagaat cggatggggc gtggtagaca aattagccct gtggtgttca gtgtcgtcaa gtcgttgaaa aaaagggcaa tccgcaccaa gttcatcttc cgctatgaac cagacaagct ggcagcggca aagatagcaa acaagataca tgagaacaca tacgtggata tcgagaacat agatgtcctt gtgctggcga atgncctcaa gaattatatc agagagcacc ttgacggcct agttcccata gaggcctttg agaagatcaa gacttgcatc atctcgaatg ataggnatac aagagaagca ttgntctcgt acatgccgtt tatatttaca gatgcagaga gacgcnngnt cacctcgctc tttgagcttc tgaagatgat tganaanaac tcggnngcga ngganangaa tatcgactcn cttctgggnc tgntcannct antcctacac ccncnngcng cgntnnnnnc cnngntgata nnatntgtcn nntntcacnn ggnantnnnc nnctcnactt nntncnnacc cnnnnnnnnn nncnnnnnnn nnnntnnngn nnnanncann nnnnntnnnc nnnnnnnnnn nnnnnnnnnn nnnnnnnngn nnnnnncc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLRDFPKKR KIFNIAEMKE IEETNARADE AIYELKASLQ TCSQACSPQV TVQPISESEA TRPLKDILLQ GGSSKDPSEG LYERLFLGSA TVQRRRTEAK GSPSTEGEKD FHEFRYELLS TSQKIELSRF CWSAFRKRAT NMLFAWVNCF QKKNRMGRGR QISPVVFSVV KSLKKRAIRT KFIFRYEPDK LAAAKIANKI HENTCVDIEN IDVLVLANAL KNYIREHLDG LVPIEAFEKI KTCIISNDRY TREALFSYMP FIFTDAERRL LTSLFELLKM IDKNSDATEM NIDSLLGLFS LVLHPQAAFT TLDDLEYVKI ITRELYQLDF DAIPQGVVVH ELVFV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctgagagat tttccgaaga aaagaaagat ctttaacata gcagagatga aggagataga ggaaacaaat gcaagggcag acgaggccat ctatgagttg aaggcctctc ttcaaacatg ttctcaggca tgcagccctc aggttacggt gcagcccatt tccgaaagcg aagccaccag acccctgaaa gacattcttc tccagggggg aagctctaag gatcccagtg aagggttgta cgagagactt tttcttggct cggcaacggt ccagaggagg aggacggaag caaaaggctc tccgtctact gagggggaaa aggacttcca tgaatttaga tatgagcttc tatcaacgtc gcagaagata gagcttagca gattttgttg gagtgcattt aggaagaggg ctacgaacat gctctttgcc tgggtgaatt gctttcagaa gaagaatcgg atggggcgtg gtagacaaat tagccctgtg gtgttcagtg tcgtcaagtc gttgaaaaaa agggcaatcc gcaccaagtt catcttccgc tatgaaccag acaagctggc agcggcaaag atagcaaaca agatacatga gaacacatgc gtggatatcg agaacataga tgtccttgtg ctggcgaatg ccctcaagaa ttatatcaga gagcaccttg acggcctagt tcccatagag gcctttgaga agatcaagac ttgcatcatc tcgaatgata ggtatacaag agaagcattg ttctcgtaca tgccgtttat atttacagat gcagagagac gcttgctcac ctcgctcttt gagcttctga agatgattga taagaactcg gatgcgacgg agatgaatat cgactcactt ctggggctgt tcagcctagt cctacaccca caggcagcgt ttacaaccct tgatgaccta gaatatgtca agattatcac gagggagtta taccaactcg actttgatgc cataccccaa ggcgttgttg tccatgagct ggtctttgtg taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa