EncuA.17079.a

Spore wall and anchoring disk complex protein EnP1

CENTER ID: EncuA.17079.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.17079.a.A2.GE27882 mature protein 17 357
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.17079.a.A2.PW29677 mature protein 17 357

External Resources

RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU01_0820
RefSeq: XP_965917.1
UniProt: Q8SWL3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKLLGFLIVG LSAISALKTK ALHLTCEQEL RPYSAVVDAN CMAFALNGSN IHEAIKYLQA MNIKKAYVLY WNDHDLRGTP MVLYDNGALA PFDPYTNTAK YVLCVEACPC PGSKAASVGG FQAATSSEKI YVEGSARPAQ CSEVCIEPVE RRPHYKKIVV NPSPSNCIPC EPECYDSSSS SECNKKRCKT FPRICKEKCG SRRRGCPRKV EVLKSQKTYT FDIEKYRRRG EVVVRVCSKD SKEKFERFIL SRNGEIRGNN NKNCILEPLP KCLRCPGQLH KLKKHIERKV CQEVCMYINA KCDIFVLVGD CDFYRVVVND RRRYRNLHLK KVRGHKLREL IKHGLFGVEF GPLDLDR
NT Sequence
atgaagctct tgggattcct gatagttgga ctcagtgcca tcagtgctct taagactaag gctcttcacc ttacatgcga gcaggagctg aggccatata gtgccgtggt agatgctaac tgcatggcgt ttgccctgaa cggaagcaac attcacgagg caatcaaata cctccaggca atgaacatca agaaggcata cgtgctctac tggaacgacc atgacctccg tggaactcca atggttcttt atgacaacgg agctcttgca cccttcgacc cgtacaccaa cacagcgaag tacgttctct gtgtcgaggc ctgcccatgc ccagggtcca aggctgccag cgttggtggg ttccaggcgg ctacctcatc cgagaagata tatgtcgagg gcagtgccag gcctgcccaa tgctccgaag tgtgcatcga gccggttgag cgccgccccc actacaagaa gatcgtcgtc aatccatctc caagcaactg cattccttgc gagcccgagt gctatgactc tagcagcagc agcgagtgca ataagaagag atgcaagaca ttcccaagga tatgcaaaga gaagtgcgga tctaggcgcc gtgggtgccc aaggaaggtc gaggtcctga agagccagaa gacatacact ttcgacattg agaagtacag aagaagggga gaggttgttg tcagagtttg ctctaaggac tccaaggaga agttcgagag attcattctg tcgaggaacg gagagattag gggaaacaac aacaagaact gcattctgga gcctcttcca aagtgcctcc gatgcccagg acagctccac aagctcaaga agcacatcga gcgcaaggtc tgccaggagg tctgcatgta catcaatgcc aagtgcgaca tctttgtgct ggtcggggac tgcgacttct acagagtcgt ggttaacgac aggagaagat acagaaacct ccacctcaag aaggttcgag gacacaagct ccgagagctt attaagcacg gactctttgg tgttgaattt ggacctctcg atctcgatcg ctga
Details for EncuA.17079.a.A2.GE27882
HARVESTED ON: 11/3/2009
SEQUENCED ON: 11/10/2009
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLKTKALHL TCEQELRPYS AVVDANCMAF ALNGSNIHEA IKYLQAMNIK KAYVLYWNDH DLRGTPMVLY DNGALAPFDP YTNTAKYVLC VEACPCPGSK AASVGGFQAA TSSEKIYVEG SARPAQCSEV CIEPVERRPH YKKIVVNPSP SNCIPCEPEC YDSSSSSECN KKRCKTFPRI CKEKCGSRRR GCPRKVEVLK SQKTYTFDIE KYRRRGEVVV RVCSKDSKEK FERFILSRNG EIRGNNNKNC ILEPLPKCLR CPGQLHKLKK HIERKVCQEV CMYINAKCDI FVLVGDCDFY RVVVNDRRRY RNLHLKKVRG HKLRELIKHG LFGVEFGPLD L
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgctta agactaaggc tcttcacctt acatgcgagc aggagctgag gccatatagt gccgtggtag atgctaactg catggcgttt gccctgaacg gaagcaacat tcacgaggca atcaaatacc tccaggcaat gaacatcaag aaggcatacg tgctctactg gaacgaccat gacctccgtg gaactccaat ggttctttat gacaacggag ctcttgcacc cttcgacccg tacaccaaca cagcgaagta cgttctctgt gtcgaggcct gcccatgccc agggtccaag gctgccagcg ttggtgggtt ccaggcggct acctcatccg agaagatata tgtcgagggc agtgccaggc ctgcccaatg ctccgaagtg tgcatcgagc cggttgagcg ccgcccccac tacaagaaga tcgtcgtcaa tccatctcca agcaactgca ttccttgcga gcccgagtgc tatgactcta gcagcagcag cgagtgcaat aagaagagat gcaagacatt cccaaggata tgcaaagaga agtgcggatc taggcgccgt gggtgcccaa ggaaggtcga ggtcctgaag agccagaaga catacacttt cgacattgag aagtacagaa gaaggggaga ggttgttgtc agagtttgct ctaaggactc caaggagaag ttcgagagat tcattctgtc gaggaacgga gagattaggg gaaacaacaa caagaactgc attctggagc ctcttccaaa gtgcctccga tgcccaggac agctccacaa gctcaagaag cacatcgagc gcaaggtctg ccaggaggtc tgcatgtaca tcaatgccaa gtgcgacatc tttgtgctgg tcggggactg cgacttctac agagtcgtgg ttaacgacag gagaagatac agaaacctcc acctcaagaa ggttcgagga cacaagctcc gagagcttat taagcacgga ctctttggtg ttgaatttgg acctctcgat ctcg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLKTKALHL TCEQELRPYS AVVDANCMAF ALNGSNIHEA IKYLQAMNIK KAYVLYWNDH DLRGTPMVLY DNGALAPFDP YTNTAKYVLC VEACPCPGSK AASVGGFQAA TSSEKIYVEG SARPAQCSEV CIEPVERRPH YKKIVVNPSP SNCIPCEPEC YDSSSSSECN KKRCKTFPRI CKEKCGSRRR GCPRKVEVLK SQKTYTFDIE KYRRRGEVVV RVCSKDSKEK FERFILSRNG EIRGNNNKNC ILEPLPKCLR CPGQLHKLKK HIERKVCQEV CMYINAKCDI FVLVGDCDFY RVVVNDRRRY RNLHLKKVRG HKLRELIKHG LFGVEFGPLD LDR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcttaagact aaggctcttc accttacatg cgagcaggag ctgaggccat atagtgccgt ggtagatgct aactgcatgg cgtttgccct gaacggaagc aacattcacg aggcaatcaa atacctccag gcaatgaaca tcaagaaggc atacgtgctc tactggaacg accatgacct ccgtggaact ccaatggttc tttatgacaa cggagctctt gcacccttcg acccgtacac caacacagcg aagtacgttc tctgtgtcga ggcctgccca tgcccagggt ccaaggctgc cagcgttggt gggttccagg cggctacctc atccgagaag atatatgtcg agggcagtgc caggcctgcc caatgctccg aagtgtgcat cgagccggtt gagcgccgcc cccactacaa gaagatcgtc gtcaatccat ctccaagcaa ctgcattcct tgcgagcccg agtgctatga ctctagcagc agcagcgagt gcaataagaa gagatgcaag acattcccaa ggatatgcaa agagaagtgc ggatctaggc gccgtgggtg cccaaggaag gtcgaggtcc tgaagagcca gaagacatac actttcgaca ttgagaagta cagaagaagg ggagaggttg ttgtcagagt ttgctctaag gactccaagg agaagttcga gagattcatt ctgtcgagga acggagagat taggggaaac aacaacaaga actgcattct ggagcctctt ccaaagtgcc tccgatgccc aggacagctc cacaagctca agaagcacat cgagcgcaag gtctgccagg aggtctgcat gtacatcaat gccaagtgcg acatctttgt gctggtcggg gactgcgact tctacagagt cgtggttaac gacaggagaa gatacagaaa cctccacctc aagaaggttc gaggacacaa gctccgagag cttattaagc acggactctt tggtgttgaa tttggacctc tcgatctcga tcgcaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for EncuA.17079.a.A2.PW29677
PURIFICATION DATe: 5/24/2010
CONCENTRATION: 11.41mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLKTKALHL TCEQELRPYS AVVDANCMAF ALNGSNIHEA IKYLQAMNIK KAYVLYWNDH DLRGTPMVLY DNGALAPFDP YTNTAKYVLC VEACPCPGSK AASVGGFQAA TSSEKIYVEG SARPAQCSEV CIEPVERRPH YKKIVVNPSP SNCIPCEPEC YDSSSSSECN KKRCKTFPRI CKEKCGSRRR GCPRKVEVLK SQKTYTFDIE KYRRRGEVVV RVCSKDSKEK FERFILSRNG EIRGNNNKNC ILEPLPKCLR CPGQLHKLKK HIERKVCQEV CMYINAKCDI FVLVGDCDFY RVVVNDRRRY RNLHLKKVRG HKLRELIKHG LFGVEFGPLD L
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgctta agactaaggc tcttcacctt acatgcgagc aggagctgag gccatatagt gccgtggtag atgctaactg catggcgttt gccctgaacg gaagcaacat tcacgaggca atcaaatacc tccaggcaat gaacatcaag aaggcatacg tgctctactg gaacgaccat gacctccgtg gaactccaat ggttctttat gacaacggag ctcttgcacc cttcgacccg tacaccaaca cagcgaagta cgttctctgt gtcgaggcct gcccatgccc agggtccaag gctgccagcg ttggtgggtt ccaggcggct acctcatccg agaagatata tgtcgagggc agtgccaggc ctgcccaatg ctccgaagtg tgcatcgagc cggttgagcg ccgcccccac tacaagaaga tcgtcgtcaa tccatctcca agcaactgca ttccttgcga gcccgagtgc tatgactcta gcagcagcag cgagtgcaat aagaagagat gcaagacatt cccaaggata tgcaaagaga agtgcggatc taggcgccgt gggtgcccaa ggaaggtcga ggtcctgaag agccagaaga catacacttt cgacattgag aagtacagaa gaaggggaga ggttgttgtc agagtttgct ctaaggactc caaggagaag ttcgagagat tcattctgtc gaggaacgga gagattaggg gaaacaacaa caagaactgc attctggagc ctcttccaaa gtgcctccga tgcccaggac agctccacaa gctcaagaag cacatcgagc gcaaggtctg ccaggaggtc tgcatgtaca tcaatgccaa gtgcgacatc tttgtgctgg tcggggactg cgacttctac agagtcgtgg ttaacgacag gagaagatac agaaacctcc acctcaagaa ggttcgagga cacaagctcc gagagcttat taagcacgga ctctttggtg ttgaatttgg acctctcgat ctcg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLKTKALHL TCEQELRPYS AVVDANCMAF ALNGSNIHEA IKYLQAMNIK KAYVLYWNDH DLRGTPMVLY DNGALAPFDP YTNTAKYVLC VEACPCPGSK AASVGGFQAA TSSEKIYVEG SARPAQCSEV CIEPVERRPH YKKIVVNPSP SNCIPCEPEC YDSSSSSECN KKRCKTFPRI CKEKCGSRRR GCPRKVEVLK SQKTYTFDIE KYRRRGEVVV RVCSKDSKEK FERFILSRNG EIRGNNNKNC ILEPLPKCLR CPGQLHKLKK HIERKVCQEV CMYINAKCDI FVLVGDCDFY RVVVNDRRRY RNLHLKKVRG HKLRELIKHG LFGVEFGPLD LDR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCTTAAGACT AAGGCTCTTC ACCTTACATG CGAGCAGGAG CTGAGGCCAT ATAGTGCCGT GGTAGATGCT AACTGCATGG CGTTTGCCCT GAACGGAAGC AACATTCACG AGGCAATCAA ATACCTCCAG GCAATGAACA TCAAGAAGGC ATACGTGCTC TACTGGAACG ACCATGACCT CCGTGGAACT CCAATGGTTC TTTATGACAA CGGAGCTCTT GCACCCTTCG ACCCGTACAC CAACACAGCG AAGTACGTTC TCTGTGTCGA GGCCTGCCCA TGCCCAGGGT CCAAGGCTGC CAGCGTTGGT GGGTTCCAGG CGGCTACCTC ATCCGAGAAG ATATATGTCG AGGGCAGTGC CAGGCCTGCC CAATGCTCCG AAGTGTGCAT CGAGCCGGTT GAGCGCCGCC CCCACTACAA GAAGATCGTC GTCAATCCAT CTCCAAGCAA CTGCATTCCT TGCGAGCCCG AGTGCTATGA CTCTAGCAGC AGCAGCGAGT GCAATAAGAA GAGATGCAAG ACATTCCCAA GGATATGCAA AGAGAAGTGC GGATCTAGGC GCCGTGGGTG CCCAAGGAAG GTCGAGGTCC TGAAGAGCCA GAAGACATAC ACTTTCGACA TTGAGAAGTA CAGAAGAAGG GGAGAGGTTG TTGTCAGAGT TTGCTCTAAG GACTCCAAGG AGAAGTTCGA GAGATTCATT CTGTCGAGGA ACGGAGAGAT TAGGGGAAAC AACAACAAGA ACTGCATTCT GGAGCCTCTT CCAAAGTGCC TCCGATGCCC AGGACAGCTC CACAAGCTCA AGAAGCACAT CGAGCGCAAG GTCTGCCAGG AGGTCTGCAT GTACATCAAT GCCAAGTGCG ACATCTTTGT GCTGGTCGGG GACTGCGACT TCTACAGAGT CGTGGTTAAC GACAGGAGAA GATACAGAAA CCTCCACCTC AAGAAGGTTC GAGGACACAA GCTCCGAGAG CTTATTAAGC ACGGACTCTT TGGTGTTGAA TTTGGACCTC TCGATCTCGA TCGCAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA