EncuA.17074.a

cyclin C

CENTER ID:	EncuA.17074.a
ORGANISM:	Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE:	Microsporidiosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EncuA.17074.a.A1.GE30656	full length		1	214

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	MicrosporidiaDB:ECU09_0840
OrthoMCL:	OG5_129660
RefSeq:	XP_955638.1
UniProt:	Q8SQN3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGSEYVKCKG VSEAKNVQDI VNISRLLGMP QKTTYFTLAI YYRYILMPKN RGTIPTSAAC ILLSGKVNGS LRSLEQILKT SYRYYGVDPG RFFQEDYQDA IDIELHACIA MDFNFDMEDP YGFLEQICMD NDIDRSRAQT VWVVLNDTMY LPFILSFSIK SIVVSCMFIS DIARGCGGGD IDKFKERYRL AGIDSADIDF ISKEIVSFYE RVSE

NT Sequence

atgggttcag agtatgtgaa gtgtaagggc gtgtctgaag ccaagaacgt tcaggacatc gtaaacatct cgagactgct gggaatgcca cagaagacca catactttac tctggcgatt tactacagat atatcctcat gccaaagaac agaggcacca ttccaacaag cgccgcatgc atattgctat ctggaaaggt caatggatcg ttgagaagcc tcgagcagat tctcaagaca agctacaggt actacggagt cgacccggga agatttttcc aggaggacta ccaggatgca atagacatcg agctccatgc ctgcattgca atggacttca actttgacat ggaggatccc tacggcttct tagagcagat atgcatggac aacgacatcg acagatcgag ggcacagacc gtgtgggtcg ttctgaacga cacgatgtat ctgcccttta tactgtcttt cagcataaaa agcattgttg tgagctgcat gttcatctca gacattgcta gaggctgcgg aggcggagac atcgataagt tcaaagaaag atacaggctt gcagggatcg acagcgcaga catcgatttt atttctaagg aaattgtgtc gttctatgag cgtgtatcgg aatag

Details for EncuA.17074.a.A1.GE30656

HARVESTED ON:	10/27/2010
SEQUENCED ON:	11/3/2010
EXPECTED MW:	26kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMGSEYVKCK GVSEAKNVQD IVNISRLLGM PQKTTYFTLA IYYRYILMPK NRGTIPTSAA CILLSGKVNG SLRSLEQILK TSYRYYGVDP GRFFQEDYQD AIDIELHACI AMDFNFDMED PYGFLEQICM DNDIDRSRAQ TVWVVLNDTM YLPFILSFSI KSIVVSCMFI SDIARGCGGG DIDKFKERYR LAGIDSADID FISKEIVSFY ERVSE

Validated NT Sequence

attttgttta ctttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgggtt cagagtatgt gaagtgtaag ggcgtgtctg aagccaagaa cgttcaggac atcgtaaaca tctcgagact gctgggaatg ccacagaaga ccacatactt tactctggcg atttactaca gatatatcct catgccaaag aacagaggca ccattccaac aagcgccgca tgcatattgc tatctggaaa ggtcaatgga tcgttgagaa gcctcgagca gattctcaag acaagctaca ggtactacgg agtcgacccg ggaagatttt tccaggagga ctaccaggat gcaatagaca tcgagctcca tgcctgcatt gcaatggact tcaactttga catggaggat ccctacggct tcttagagca gatatgcatg gacaacgaca tcgacagatc gagggcacag accgtgtggg tcgttctgaa cgacacgatg tatctgccct ttatactgtc tttcagcata aaaagcattg ttgtgagctg catgttcatc tcagacattg ctagaggctg cggaggcgga gacatcgata agttcaaaga aagatacagg cttgcaggga tcgacagcgc agacatcgat tttatttcta aggaaattgt gtcgttctat gagcgtgtat cggaataata aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttnggct gctgccaccg ctnannnnnn nannnnannn nnnaaaaann naaannnntn aannntnnnn nnnnnnnnna nnngnnntnt angntnnann nnnnnnnnnn nggggnnnnn nnt

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggttcagag tatgtgaagt gtaagggcgt gtctgaagcc aagaacgttc aggacatcgt aaacatctcg agactgctgg gaatgccaca gaagaccaca tactttactc tggcgattta ctacagatat atcctcatgc caaagaacag aggcaccatt ccaacaagcg ccgcatgcat attgctatct ggaaaggtca atggatcgtt gagaagcctc gagcagattc tcaagacaag ctacaggtac tacggagtcg acccgggaag atttttccag gaggactacc aggatgcaat agacatcgag ctccatgcct gcattgcaat ggacttcaac tttgacatgg aggatcccta cggcttctta gagcagatat gcatggacaa cgacatcgac agatcgaggg cacagaccgt gtgggtcgtt ctgaacgaca cgatgtatct gccctttata ctgtctttca gcataaaaag cattgttgtg agctgcatgt tcatctcaga cattgctaga ggctgcggag gcggagacat cgataagttc aaagaaagat acaggcttgc agggatcgac agcgcagaca tcgattttat ttctaaggaa attgtgtcgt tctatgagcg tgtatcggaa aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa