EncuA.17072.a

G2/MITOTIC SPECIFIC CYCLIN 2

CENTER ID: EncuA.17072.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
EncuA.17072.a.MB1.GE30478 full length 1 300
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU08_0260
OrthoMCL: OG5_126649
RefSeq: NP_597155.1
UniProt: Q8SRF2
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVVSFRKSEP LKNVTNKIGR RAEKTVLPLT KMEEIVYKQQ RVGEASQIVM VDKYSDDVFE YFSTIDKPFV YINDGVSWKM RSLLVDWIID IHDKLGLCHD TLFLAINFID RFLSMRSIPS SKLQLVGISA LMIACKYEEV VCPALQTLVL LTEKTLTGED VRKAEKYMLH TLNYDLQYVS PLNFLRKCSK ANNYEICSRT VGKYLLELMT LYEEFLRFKG SVRAAAAMYL ARKITSQDQC KNLFIMYSGH TKDDIKECFN ALVHTISESI PFENIQNKYN TPKFLHAASF VKEYAEKNFK
NT Sequence
atggtggtct cgttcaggaa atcagagcct ttgaagaatg tgacaaacaa gatcggaagg agagccgaaa agacagtcct ccctctcacg aagatggagg aaattgttta caagcagcag agggttggag aggcctccca gatagtgatg gtggacaagt actcggatga tgttttcgag tacttctcaa caatcgataa acctttcgta tatatcaacg acggagtgtc gtggaagatg aggagcctct tagtagactg gatcatcgac atccatgaca agctgggact ctgccacgac actcttttcc ttgcaatcaa cttcattgac aggttccttt ccatgaggtc gattcctagc tcgaagctcc agcttgtcgg gatttccgcc ctgatgatag catgcaagta tgaggaggtt gtgtgcccag ctcttcagac gcttgttctt ctaacagaga agacgttgac gggagaggat gtaaggaagg cggagaagta catgctccat acccttaact acgatctgca gtatgtgtct cctctgaatt tcctgaggaa atgctccaag gccaacaact acgagatctg cagtaggaca gtcggaaagt atttattaga actcatgacc ctctacgagg agttcctaag attcaaagga tccgtgaggg ctgcagcagc aatgtatctc gcaagaaaga taacgtccca ggatcaatgc aagaacctct ttatcatgta ctccggacat actaaagatg acataaagga atgcttcaat gcccttgtcc acaccataag cgagagcatt ccattcgaaa acatacagaa caaatacaat acccccaagt tccttcatgc agcatccttt gtcaaggagt atgcagagaa aaacttcaag taa
Details for EncuA.17072.a.MB1.GE30478
HARVESTED ON: 5/11/2010
SEQUENCED ON: 10/7/2010
EXPECTED MW: 79kDa
OBSERVED MW: 80kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 36
Validated AA Sequence
MVVSFRKSEP LKNVTNKIGR RAEKTVLPLT KMEEIVYKQQ RVGEASQIVM VDKYSDDVFE YFSTIDKPFV YINDGVSWKM RSLLVDWIID IHDKLGLCHD TLFLAINFID RFLSMRSIPS SKLQLVGISA LMIACKYEEV VCPALQTLVL LTEKTLTGED VRKAEKYMLH TLNYDLQYVS PLNFLRKCSK ANNYEICSRT VGKYLLELMT LYEEXLRFKG SVRAAAAMYL ARKITSQDQC KNLFIMYSGH TKDDIKEC
Validated NT Sequence
gaagcattcc tttatgtcat ctttagtatg tccggagtac atgataaaga ggttcttgca ttgatcctgg gacgttatct ttcttgcgag atacattgct gctgcagccc tcacggatcc tttgaatctt agnaactcct cgtagagggt catgagttct aataaatact ttccgactgt cctactgcag atctcgtagt tgttggcctt ggagcatttc ctcaggaaat tcagaggaga cacatactgc agatcgtagt taagggtatg gagcatgtac ttctccgcct tccttacatc ctctcccgtc aacgtcttct ctgttagaag aacaagcgtc tgaagagctg ggcacacaac ctcctcatac ttgcatgcta tcatcagggc ggaaatcccg acaagctgga gcttcgagct aggaatcgac ctcatggaaa ggaacctgtc aatgaagttg attgcaagga aaagagtgtc gtggcagagt cccagcttgt catggatgtc gatgatccag tctactaaga ggctcctcat cttccacgac actccgtcgt tgatatatac gaaaggttta tcgattgttg agaagtactc gaaaacatca tccgagtact tgtccaccat cactatctgg gaggcctctc caaccctctg ctgcttgtaa acaatttcct ccatcttcgt gagagggagg actgtctttt cggctctcct tccgatcttg tttgtcacat tcttcaaagg ctctgatttc ctgaacgaga ccaccatcga accaggaccc nng
Expected Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS MVVSFRKSEP LKNVTNKIGR RAEKTVLPLT KMEEIVYKQQ RVGEASQIVM VDKYSDDVFE YFSTIDKPFV YINDGVSWKM RSLLVDWIID IHDKLGLCHD TLFLAINFID RFLSMRSIPS SKLQLVGISA LMIACKYEEV VCPALQTLVL LTEKTLTGED VRKAEKYMLH TLNYDLQYVS PLNFLRKCSK ANNYEICSRT VGKYLLELMT LYEEFLRFKG SVRAAAAMYL ARKITSQDQC KNLFIMYSGH TKDDIKECFN ALVHTISESI PFENIQNKYN TPKFLHAASF VKEYAEKNFK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcgatggt ggtctcgttc aggaaatcag agcctttgaa gaatgtgaca aacaagatcg gaaggagagc cgaaaagaca gtcctccctc tcacgaagat ggaggaaatt gtttacaagc agcagagggt tggagaggcc tcccagatag tgatggtgga caagtactcg gatgatgttt tcgagtactt ctcaacaatc gataaacctt tcgtatatat caacgacgga gtgtcgtgga agatgaggag cctcttagta gactggatca tcgacatcca tgacaagctg ggactctgcc acgacactct tttccttgca atcaacttca ttgacaggtt cctttccatg aggtcgattc ctagctcgaa gctccagctt gtcgggattt ccgccctgat gatagcatgc aagtatgagg aggttgtgtg cccagctctt cagacgcttg ttcttctaac agagaagacg ttgacgggag aggatgtaag gaaggcggag aagtacatgc tccataccct taactacgat ctgcagtatg tgtctcctct gaatttcctg aggaaatgct ccaaggccaa caactacgag atctgcagta ggacagtcgg aaagtattta ttagaactca tgaccctcta cgaggagttc ctaagattca aaggatccgt gagggctgca gcagcaatgt atctcgcaag aaagataacg tcccaggatc aatgcaagaa cctctttatc atgtactccg gacatactaa agatgacata aaggaatgct tcaatgccct tgtccacacc ataagcgaga gcattccatt cgaaaacata cagaacaaat acaatacccc caagttcctt catgcagcat cctttgtcaa ggagtatgca gagaaaaact tcaagtaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa