EncuA.17066.a

polar tube protein

CENTER ID: EncuA.17066.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.17066.a.A2.GE27880 mature protein 19 277
EncuA.17066.a.A2.GE28374 mature protein 19 277
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.17066.a.A2.PW27771 mature protein 19 277
EncuA.17066.a.A2.PW28270 mature protein 19 277
EncuA.17066.a.A2.PW29194 mature protein 19 277

External Resources

RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU06_0240
RefSeq: NP_585780.1
UniProt: Q8SRT0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLLLLAITAV VSATMVHPSA VVPQPAAPLH VVPPQQQMGM VNGCTSKKLE GAEIMRRNMI ECQKRSSEAT KAMIERANEK AVESFNKEVS KGPSQKDGGQ CIEKAVQGTD RCILAGIIDK AVNKRKYRIS DVENSTSLYR GDKLIALIVN VDYGLQPITK PKKKKSKIMA NLPQPKREMY FNQIGQLVGA RGTFPQENKE DCKPCEGPKK TVETTSEKCN LGCELKGTSA LISKAIQKKE VKDTKEGEKS ASQDSDGEGT AEDAEVQQPS ADGEGLE
NT Sequence
atgttgttac ttctcgccat aactgctgtt gttagcgcca cgatggtcca tccttcagct gttgttccac agcccgcagc acctctccat gtcgttcccc cacagcagca aatgggcatg gttaacggat gcaccagcaa gaaactagag ggtgcagaaa taatgagaag gaacatgatt gagtgccaga aaagaagctc ggaggcaaca aaggcgatga ttgaaagggc aaatgaaaag gctgtagaat cattcaacaa ggaagttagc aaaggaccta gccaaaagga tggaggccag tgcatagaaa aagctgtaca aggtaccgat aggtgtattc tcgctggaat aatcgataag gcggtgaaca agcgcaagta cagaatctca gatgtggaga acagcacctc gctctacaga ggagacaagc taattgccct aattgtcaat gtcgactatg ggctgcagcc gatcactaag ccaaagaaga agaagtccaa gataatggcg aatctccctc agccgaagag agagatgtat ttcaaccaaa tcggtcagct tgttggagca agaggaacgt tcccccagga aaacaaggag gactgcaagc cttgtgaggg tcccaagaag actgttgaaa ctacttctga gaaatgtaat cttgggtgcg agcttaaagg aacatctgct ctgataagca aggccataca gaagaaggaa gtcaaggaca cgaaggaagg ggagaaaagt gcaagccagg actctgatgg cgagggcact gctgaggatg cggaagtaca gcaaccttct gcggacggcg agggtctaga gtaa
Details for EncuA.17066.a.A2.GE27880
HARVESTED ON: 11/3/2009
SEQUENCED ON: 11/10/2009
EXPECTED MW: 30kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSAVVPQPA APLHVVPPQQ QMGMVNGCTS KKLEGAEIMR RNMIECQKRS SEATKAMIER ANEKAVESFN KEVSKGPSQK DGGQCIEKAV QGTDRCILAG IIDKAVNKRK YRISDVENST SLYRGDKLIA LIVNVDYGLQ PITKPKKKKS KIMANLPQPK REMYFNQIGQ LVGARGTFPQ ENKEDCKPCE GPKKTVETTS EKCNLGCELK GTSALISKAI QKKEVKDTKE GEKSASQDSD GEGTAEDAEV QQPSADGEGL E
Validated NT Sequence
ttattactct agaccctcgc cgtccgcaga aggttgctgt acttccgcat cctcagcagt gccctcgcca tcagagtcct ggcttgcact tttctcccct tccttcgtgt ccttgacttc cttcttctgt atggccttgc ttatcagagc agatgttcct ttaagctcgc acccaagatt acatttctca gaagtagttt caacagtctt cttgggaccc tcacaaggct tgcagtcctc cttgttttcc tgggggaacg ttcctcttgc tccaacaagc tgaccgattt ggttgaaata catctctctc ttcggctgag ggagattcgc cattatcttg gacttcttct tctttggctt agtgatcggc tgcagcccat agtcgacatt gacaattagg gcaattagct tgtctcccct gtagagcgag gtgctgttct ccacatctga gattctgtac ttgcgcttgt tcaccgcctt atcgattatt ccagcgagaa tacacctatc ggtaccttgt acagcttttt ctatgcactg gcctccatcc ttttggctag gtcctttgct aacttccttg ttgaatgatt ctacagcctt ttcatttgcc ctttcaatca tcgcctttgt tgcctccgag cttcttttct ggcactcaat catgttcctt ctcattattt ctgcaccctc tagtttcttg ctggtgcatc cgttaaccat gcccatttgc tgctgtgggg gaacgacatg gagaggtgct gcgggctgtg gaacaacagc tgacatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSAVVPQPA APLHVVPPQQ QMGMVNGCTS KKLEGAEIMR RNMIECQKRS SEATKAMIER ANEKAVESFN KEVSKGPSQK DGGQCIEKAV QGTDRCILAG IIDKAVNKRK YRISDVENST SLYRGDKLIA LIVNVDYGLQ PITKPKKKKS KIMANLPQPK REMYFNQIGQ LVGARGTFPQ ENKEDCKPCE GPKKTVETTS EKCNLGCELK GTSALISKAI QKKEVKDTKE GEKSASQDSD GEGTAEDAEV QQPSADGEGL E
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcagctgtt gttccacagc ccgcagcacc tctccatgtc gttcccccac agcagcaaat gggcatggtt aacggatgca ccagcaagaa actagagggt gcagaaataa tgagaaggaa catgattgag tgccagaaaa gaagctcgga ggcaacaaag gcgatgattg aaagggcaaa tgaaaaggct gtagaatcat tcaacaagga agttagcaaa ggacctagcc aaaaggatgg aggccagtgc atagaaaaag ctgtacaagg taccgatagg tgtattctcg ctggaataat cgataaggcg gtgaacaagc gcaagtacag aatctcagat gtggagaaca gcacctcgct ctacagagga gacaagctaa ttgccctaat tgtcaatgtc gactatgggc tgcagccgat cactaagcca aagaagaaga agtccaagat aatggcgaat ctccctcagc cgaagagaga gatgtatttc aaccaaatcg gtcagcttgt tggagcaaga ggaacgttcc cccaggaaaa caaggaggac tgcaagcctt gtgagggtcc caagaagact gttgaaacta cttctgagaa atgtaatctt gggtgcgagc ttaaaggaac atctgctctg ataagcaagg ccatacagaa gaaggaagtc aaggacacga aggaagggga gaaaagtgca agccaggact ctgatggcga gggcactgct gaggatgcgg aagtacagca accttctgcg gacggcgagg gtctagagaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for EncuA.17066.a.A2.GE28374
HARVESTED ON: 1/5/2010
SEQUENCED ON: 1/15/2010
EXPECTED MW: 30kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSAVVPQPA APLHVVPPQQ QMGMVNGCTS KKLEGAEIMR RNMIECQKRS SEATKAMIER ANEKAVESFN KEVSKGPSQK DGGQCIEKAV QGTDRCILAG IIDKAVNKRK YRISDVENST SLYRGDKLIA LIVNVDYGLQ PITKPKKKKS KIMANLPQPK REMYFNQIGQ LVGARGTFPQ ENKEDCKPCE GPKKTVETTS EKCNLGCELK GTSALISKAI QKKEVKDTKE GEKSASQDSD GEGTAEDAEV QQPSADGEGL E
Validated NT Sequence
ttattactct agaccctcgc cgtccgcaga aggttgctgt acttccgcat cctcagcagt gccctcgcca tcagagtcct ggcttgcact tttctcccct tccttcgtgt ccttgacttc cttcttctgt atggccttgc ttatcagagc agatgttcct ttaagctcgc acccaagatt acatttctca gaagtagttt caacagtctt cttgggaccc tcacaaggct tgcagtcctc cttgttttcc tgggggaacg ttcctcttgc tccaacaagc tgaccgattt ggttgaaata catctctctc ttcggctgag ggagattcgc cattatcttg gacttcttct tctttggctt agtgatcggc tgcagcccat agtcgacatt gacaattagg gcaattagct tgtctcccct gtagagcgag gtgctgttct ccacatctga gattctgtac ttgcgcttgt tcaccgcctt atcgattatt ccagcgagaa tacacctatc ggtaccttgt acagcttttt ctatgcactg gcctccatcc ttttggctag gtcctttgct aacttccttg ttgaatgatt ctacagcctt ttcatttgcc ctttcaatca tcgcctttgt tgcctccgag cttcttttct ggcactcaat catgttcctt ctcattattt ctgcaccctc tagtttcttg ctggtgcatc cgttaaccat gcccatttgc tgctgtgggg gaacgacatg gagaggtgct gcgggctgtg gaacaacagc tgacatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSAVVPQPA APLHVVPPQQ QMGMVNGCTS KKLEGAEIMR RNMIECQKRS SEATKAMIER ANEKAVESFN KEVSKGPSQK DGGQCIEKAV QGTDRCILAG IIDKAVNKRK YRISDVENST SLYRGDKLIA LIVNVDYGLQ PITKPKKKKS KIMANLPQPK REMYFNQIGQ LVGARGTFPQ ENKEDCKPCE GPKKTVETTS EKCNLGCELK GTSALISKAI QKKEVKDTKE GEKSASQDSD GEGTAEDAEV QQPSADGEGL E
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcagctgtt gttccacagc ccgcagcacc tctccatgtc gttcccccac agcagcaaat gggcatggtt aacggatgca ccagcaagaa actagagggt gcagaaataa tgagaaggaa catgattgag tgccagaaaa gaagctcgga ggcaacaaag gcgatgattg aaagggcaaa tgaaaaggct gtagaatcat tcaacaagga agttagcaaa ggacctagcc aaaaggatgg aggccagtgc atagaaaaag ctgtacaagg taccgatagg tgtattctcg ctggaataat cgataaggcg gtgaacaagc gcaagtacag aatctcagat gtggagaaca gcacctcgct ctacagagga gacaagctaa ttgccctaat tgtcaatgtc gactatgggc tgcagccgat cactaagcca aagaagaaga agtccaagat aatggcgaat ctccctcagc cgaagagaga gatgtatttc aaccaaatcg gtcagcttgt tggagcaaga ggaacgttcc cccaggaaaa caaggaggac tgcaagcctt gtgagggtcc caagaagact gttgaaacta cttctgagaa atgtaatctt gggtgcgagc ttaaaggaac atctgctctg ataagcaagg ccatacagaa gaaggaagtc aaggacacga aggaagggga gaaaagtgca agccaggact ctgatggcga gggcactgct gaggatgcgg aagtacagca accttctgcg gacggcgagg gtctagagaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for EncuA.17066.a.A2.PW27771
PURIFICATION DATe: 3/15/2010
CONCENTRATION: 14.15mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 10
VIAL VOLUME: 50µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSAVVPQPA APLHVVPPQQ QMGMVNGCTS KKLEGAEIMR RNMIECQKRS SEATKAMIER ANEKAVESFN KEVSKGPSQK DGGQCIEKAV QGTDRCILAG IIDKAVNKRK YRISDVENST SLYRGDKLIA LIVNVDYGLQ PITKPKKKKS KIMANLPQPK REMYFNQIGQ LVGARGTFPQ ENKEDCKPCE GPKKTVETTS EKCNLGCELK GTSALISKAI QKKEVKDTKE GEKSASQDSD GEGTAEDAEV QQPSADGEGL E
Validated NT Sequence
ttattactct agaccctcgc cgtccgcaga aggttgctgt acttccgcat cctcagcagt gccctcgcca tcagagtcct ggcttgcact tttctcccct tccttcgtgt ccttgacttc cttcttctgt atggccttgc ttatcagagc agatgttcct ttaagctcgc acccaagatt acatttctca gaagtagttt caacagtctt cttgggaccc tcacaaggct tgcagtcctc cttgttttcc tgggggaacg ttcctcttgc tccaacaagc tgaccgattt ggttgaaata catctctctc ttcggctgag ggagattcgc cattatcttg gacttcttct tctttggctt agtgatcggc tgcagcccat agtcgacatt gacaattagg gcaattagct tgtctcccct gtagagcgag gtgctgttct ccacatctga gattctgtac ttgcgcttgt tcaccgcctt atcgattatt ccagcgagaa tacacctatc ggtaccttgt acagcttttt ctatgcactg gcctccatcc ttttggctag gtcctttgct aacttccttg ttgaatgatt ctacagcctt ttcatttgcc ctttcaatca tcgcctttgt tgcctccgag cttcttttct ggcactcaat catgttcctt ctcattattt ctgcaccctc tagtttcttg ctggtgcatc cgttaaccat gcccatttgc tgctgtgggg gaacgacatg gagaggtgct gcgggctgtg gaacaacagc tgacatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSAVVPQPA APLHVVPPQQ QMGMVNGCTS KKLEGAEIMR RNMIECQKRS SEATKAMIER ANEKAVESFN KEVSKGPSQK DGGQCIEKAV QGTDRCILAG IIDKAVNKRK YRISDVENST SLYRGDKLIA LIVNVDYGLQ PITKPKKKKS KIMANLPQPK REMYFNQIGQ LVGARGTFPQ ENKEDCKPCE GPKKTVETTS EKCNLGCELK GTSALISKAI QKKEVKDTKE GEKSASQDSD GEGTAEDAEV QQPSADGEGL E
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCAGCTGTT GTTCCACAGC CCGCAGCACC TCTCCATGTC GTTCCCCCAC AGCAGCAAAT GGGCATGGTT AACGGATGCA CCAGCAAGAA ACTAGAGGGT GCAGAAATAA TGAGAAGGAA CATGATTGAG TGCCAGAAAA GAAGCTCGGA GGCAACAAAG GCGATGATTG AAAGGGCAAA TGAAAAGGCT GTAGAATCAT TCAACAAGGA AGTTAGCAAA GGACCTAGCC AAAAGGATGG AGGCCAGTGC ATAGAAAAAG CTGTACAAGG TACCGATAGG TGTATTCTCG CTGGAATAAT CGATAAGGCG GTGAACAAGC GCAAGTACAG AATCTCAGAT GTGGAGAACA GCACCTCGCT CTACAGAGGA GACAAGCTAA TTGCCCTAAT TGTCAATGTC GACTATGGGC TGCAGCCGAT CACTAAGCCA AAGAAGAAGA AGTCCAAGAT AATGGCGAAT CTCCCTCAGC CGAAGAGAGA GATGTATTTC AACCAAATCG GTCAGCTTGT TGGAGCAAGA GGAACGTTCC CCCAGGAAAA CAAGGAGGAC TGCAAGCCTT GTGAGGGTCC CAAGAAGACT GTTGAAACTA CTTCTGAGAA ATGTAATCTT GGGTGCGAGC TTAAAGGAAC ATCTGCTCTG ATAAGCAAGG CCATACAGAA GAAGGAAGTC AAGGACACGA AGGAAGGGGA GAAAAGTGCA AGCCAGGACT CTGATGGCGA GGGCACTGCT GAGGATGCGG AAGTACAGCA ACCTTCTGCG GACGGCGAGG GTCTAGAGAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA
Details for EncuA.17066.a.A2.PW28270
PURIFICATION DATe: 5/24/2010
CONCENTRATION: 27.4mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSAVVPQPA APLHVVPPQQ QMGMVNGCTS KKLEGAEIMR RNMIECQKRS SEATKAMIER ANEKAVESFN KEVSKGPSQK DGGQCIEKAV QGTDRCILAG IIDKAVNKRK YRISDVENST SLYRGDKLIA LIVNVDYGLQ PITKPKKKKS KIMANLPQPK REMYFNQIGQ LVGARGTFPQ ENKEDCKPCE GPKKTVETTS EKCNLGCELK GTSALISKAI QKKEVKDTKE GEKSASQDSD GEGTAEDAEV QQPSADGEGL E
Validated NT Sequence
ttattactct agaccctcgc cgtccgcaga aggttgctgt acttccgcat cctcagcagt gccctcgcca tcagagtcct ggcttgcact tttctcccct tccttcgtgt ccttgacttc cttcttctgt atggccttgc ttatcagagc agatgttcct ttaagctcgc acccaagatt acatttctca gaagtagttt caacagtctt cttgggaccc tcacaaggct tgcagtcctc cttgttttcc tgggggaacg ttcctcttgc tccaacaagc tgaccgattt ggttgaaata catctctctc ttcggctgag ggagattcgc cattatcttg gacttcttct tctttggctt agtgatcggc tgcagcccat agtcgacatt gacaattagg gcaattagct tgtctcccct gtagagcgag gtgctgttct ccacatctga gattctgtac ttgcgcttgt tcaccgcctt atcgattatt ccagcgagaa tacacctatc ggtaccttgt acagcttttt ctatgcactg gcctccatcc ttttggctag gtcctttgct aacttccttg ttgaatgatt ctacagcctt ttcatttgcc ctttcaatca tcgcctttgt tgcctccgag cttcttttct ggcactcaat catgttcctt ctcattattt ctgcaccctc tagtttcttg ctggtgcatc cgttaaccat gcccatttgc tgctgtgggg gaacgacatg gagaggtgct gcgggctgtg gaacaacagc tgacatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSAVVPQPA APLHVVPPQQ QMGMVNGCTS KKLEGAEIMR RNMIECQKRS SEATKAMIER ANEKAVESFN KEVSKGPSQK DGGQCIEKAV QGTDRCILAG IIDKAVNKRK YRISDVENST SLYRGDKLIA LIVNVDYGLQ PITKPKKKKS KIMANLPQPK REMYFNQIGQ LVGARGTFPQ ENKEDCKPCE GPKKTVETTS EKCNLGCELK GTSALISKAI QKKEVKDTKE GEKSASQDSD GEGTAEDAEV QQPSADGEGL E
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCAGCTGTT GTTCCACAGC CCGCAGCACC TCTCCATGTC GTTCCCCCAC AGCAGCAAAT GGGCATGGTT AACGGATGCA CCAGCAAGAA ACTAGAGGGT GCAGAAATAA TGAGAAGGAA CATGATTGAG TGCCAGAAAA GAAGCTCGGA GGCAACAAAG GCGATGATTG AAAGGGCAAA TGAAAAGGCT GTAGAATCAT TCAACAAGGA AGTTAGCAAA GGACCTAGCC AAAAGGATGG AGGCCAGTGC ATAGAAAAAG CTGTACAAGG TACCGATAGG TGTATTCTCG CTGGAATAAT CGATAAGGCG GTGAACAAGC GCAAGTACAG AATCTCAGAT GTGGAGAACA GCACCTCGCT CTACAGAGGA GACAAGCTAA TTGCCCTAAT TGTCAATGTC GACTATGGGC TGCAGCCGAT CACTAAGCCA AAGAAGAAGA AGTCCAAGAT AATGGCGAAT CTCCCTCAGC CGAAGAGAGA GATGTATTTC AACCAAATCG GTCAGCTTGT TGGAGCAAGA GGAACGTTCC CCCAGGAAAA CAAGGAGGAC TGCAAGCCTT GTGAGGGTCC CAAGAAGACT GTTGAAACTA CTTCTGAGAA ATGTAATCTT GGGTGCGAGC TTAAAGGAAC ATCTGCTCTG ATAAGCAAGG CCATACAGAA GAAGGAAGTC AAGGACACGA AGGAAGGGGA GAAAAGTGCA AGCCAGGACT CTGATGGCGA GGGCACTGCT GAGGATGCGG AAGTACAGCA ACCTTCTGCG GACGGCGAGG GTCTAGAGAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA
Details for EncuA.17066.a.A2.PW29194
PURIFICATION DATe: 3/15/2010
CONCENTRATION: 11.36mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 50µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSAVVPQPA APLHVVPPQQ QMGMVNGCTS KKLEGAEIMR RNMIECQKRS SEATKAMIER ANEKAVESFN KEVSKGPSQK DGGQCIEKAV QGTDRCILAG IIDKAVNKRK YRISDVENST SLYRGDKLIA LIVNVDYGLQ PITKPKKKKS KIMANLPQPK REMYFNQIGQ LVGARGTFPQ ENKEDCKPCE GPKKTVETTS EKCNLGCELK GTSALISKAI QKKEVKDTKE GEKSASQDSD GEGTAEDAEV QQPSADGEGL E
Validated NT Sequence
ttattactct agaccctcgc cgtccgcaga aggttgctgt acttccgcat cctcagcagt gccctcgcca tcagagtcct ggcttgcact tttctcccct tccttcgtgt ccttgacttc cttcttctgt atggccttgc ttatcagagc agatgttcct ttaagctcgc acccaagatt acatttctca gaagtagttt caacagtctt cttgggaccc tcacaaggct tgcagtcctc cttgttttcc tgggggaacg ttcctcttgc tccaacaagc tgaccgattt ggttgaaata catctctctc ttcggctgag ggagattcgc cattatcttg gacttcttct tctttggctt agtgatcggc tgcagcccat agtcgacatt gacaattagg gcaattagct tgtctcccct gtagagcgag gtgctgttct ccacatctga gattctgtac ttgcgcttgt tcaccgcctt atcgattatt ccagcgagaa tacacctatc ggtaccttgt acagcttttt ctatgcactg gcctccatcc ttttggctag gtcctttgct aacttccttg ttgaatgatt ctacagcctt ttcatttgcc ctttcaatca tcgcctttgt tgcctccgag cttcttttct ggcactcaat catgttcctt ctcattattt ctgcaccctc tagtttcttg ctggtgcatc cgttaaccat gcccatttgc tgctgtgggg gaacgacatg gagaggtgct gcgggctgtg gaacaacagc tgacatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSAVVPQPA APLHVVPPQQ QMGMVNGCTS KKLEGAEIMR RNMIECQKRS SEATKAMIER ANEKAVESFN KEVSKGPSQK DGGQCIEKAV QGTDRCILAG IIDKAVNKRK YRISDVENST SLYRGDKLIA LIVNVDYGLQ PITKPKKKKS KIMANLPQPK REMYFNQIGQ LVGARGTFPQ ENKEDCKPCE GPKKTVETTS EKCNLGCELK GTSALISKAI QKKEVKDTKE GEKSASQDSD GEGTAEDAEV QQPSADGEGL E
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCAGCTGTT GTTCCACAGC CCGCAGCACC TCTCCATGTC GTTCCCCCAC AGCAGCAAAT GGGCATGGTT AACGGATGCA CCAGCAAGAA ACTAGAGGGT GCAGAAATAA TGAGAAGGAA CATGATTGAG TGCCAGAAAA GAAGCTCGGA GGCAACAAAG GCGATGATTG AAAGGGCAAA TGAAAAGGCT GTAGAATCAT TCAACAAGGA AGTTAGCAAA GGACCTAGCC AAAAGGATGG AGGCCAGTGC ATAGAAAAAG CTGTACAAGG TACCGATAGG TGTATTCTCG CTGGAATAAT CGATAAGGCG GTGAACAAGC GCAAGTACAG AATCTCAGAT GTGGAGAACA GCACCTCGCT CTACAGAGGA GACAAGCTAA TTGCCCTAAT TGTCAATGTC GACTATGGGC TGCAGCCGAT CACTAAGCCA AAGAAGAAGA AGTCCAAGAT AATGGCGAAT CTCCCTCAGC CGAAGAGAGA GATGTATTTC AACCAAATCG GTCAGCTTGT TGGAGCAAGA GGAACGTTCC CCCAGGAAAA CAAGGAGGAC TGCAAGCCTT GTGAGGGTCC CAAGAAGACT GTTGAAACTA CTTCTGAGAA ATGTAATCTT GGGTGCGAGC TTAAAGGAAC ATCTGCTCTG ATAAGCAAGG CCATACAGAA GAAGGAAGTC AAGGACACGA AGGAAGGGGA GAAAAGTGCA AGCCAGGACT CTGATGGCGA GGGCACTGCT GAGGATGCGG AAGTACAGCA ACCTTCTGCG GACGGCGAGG GTCTAGAGAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA