EncuA.10272.a

HEXOKINASE

CENTER ID: EncuA.10272.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.10272.a.A2.GE28387 Mature protein( EncuA.10272.a ) 25 475
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.10272.a.A2.PS00636 Mature protein( EncuA.10272.a ) 25 475

External Resources

RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU11_1540
RefSeq: NP_586460.1
UniProt: Q8SU17

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFTMHLAKVF AAFIAGTLGM WPALAVSTEE AVKASLFPME DFEIMEIRKA FIERITRVRE DASSGDFLDS HIDLSKARMP YQESRSVLVI DVGGTSLKIS EVVIDLGSDE KGNSPSFHDI VECSPSFYRY PDTSGVSGQD RISWNEWVAD QVVEFYGRNP PRSKVNASLT FSYPLIQTSV NNAQIKKTTK NFCFREDEAT FTTDIVEALN SSLRSRKINV CVNCVLNDST ATYMAGVLRG YKNIIGIVLG TGTNSSFCVK KGRSNEVVLY NSEWGSTRVP RSMLTEADLA VVVDLEMRKI SHNIIDVLAG GCKLGDIVLH KLRNTYPEIY EEYAEREEVL HSMIHSAITG SGNGALAKSP KLRNALMPIV RDFRARGMKI LASLIGAVVE STMDNANTIT LVLNGTTFSN AGLRDALGME VKRMHPDIEI NLVFLSNASL EGSALVSLMY SEYPKVECDL APLAAPVLSF ADQTP
NT Sequence
ATGTTTACTA TGCATTTAGC TAAAGTATTT GCTGCTTTCA TAGCTGGGAC ACTTGGTATG TGGCCTGCGC TAGCTGTAAG TACGGAGGAG GCTGTTAAGG CCAGCTTATT TCCCATGGAG GACTTCGAGA TCATGGAGAT AAGAAAAGCC TTTATTGAAA GGATCACAAG GGTACGGGAA GATGCTTCTA GTGGAGATTT TCTGGATTCC CATATCGATC TTAGTAAAGC AAGGATGCCA TATCAGGAGA GCCGCTCAGT ACTAGTTATA GATGTCGGAG GAACAAGTCT GAAGATAAGT GAGGTTGTCA TCGACCTTGG TTCTGACGAA AAGGGAAACT CTCCGTCTTT CCACGACATA GTGGAATGTA GCCCATCTTT CTACAGATAT CCTGACACCT CGGGTGTTTC GGGCCAAGAC AGGATAAGCT GGAACGAGTG GGTTGCAGAC CAGGTTGTTG AATTCTATGG CAGAAACCCT CCAAGAAGTA AGGTTAATGC ATCTCTGACA TTCTCCTATC CTCTTATCCA GACATCTGTT AATAACGCAC AAATAAAAAA GACAACAAAG AACTTCTGCT TCAGAGAGGA CGAGGCCACG TTTACCACGG ACATAGTCGA GGCCCTCAAC TCCTCGCTGA GGAGCAGGAA GATCAACGTC TGTGTCAACT GCGTCTTAAA TGACTCCACA GCAACATACA TGGCAGGAGT GCTCAGGGGA TATAAGAACA TAATCGGGAT TGTTCTTGGG ACAGGGACAA ACTCGTCCTT CTGTGTGAAG AAGGGCAGAA GCAATGAGGT GGTACTATAC AACTCCGAAT GGGGATCCAC AAGAGTTCCA AGGAGCATGT TAACAGAAGC AGACCTTGCC GTTGTTGTGG ACCTGGAAAT GAGAAAGATT TCGCATAACA TCATTGACGT CTTAGCAGGC GGCTGTAAAC TTGGTGACAT CGTTCTTCAC AAACTTAGAA ATACGTATCC TGAAATTTAT GAGGAATACG CAGAGAGGGA AGAGGTGCTC CATTCGATGA TCCACTCAGC CATCACCGGA TCTGGAAACG GCGCACTGGC TAAAAGCCCC AAACTACGCA ACGCACTGAT GCCCATAGTT CGTGACTTTA GGGCCAGGGG AATGAAGATA TTGGCATCCT TAATAGGTGC TGTGGTCGAA TCCACCATGG ATAATGCAAA CACAATCACG CTCGTTCTGA ACGGAACAAC CTTCTCGAAT GCAGGCCTCA GAGATGCTCT TGGAATGGAG GTAAAAAGGA TGCATCCGGA TATCGAGATC AACCTTGTCT TCCTTAGTAA TGCCTCTCTG GAAGGATCTG CACTTGTATC CCTTATGTAT TCCGAGTACC CCAAGGTAGA ATGTGACTTG GCACCACTTG CCGCTCCAGT CCTTTCATTT GCCGATCAGA CTCCT
Details for EncuA.10272.a.A2.GE28387
HARVESTED ON: 2/2/2010
SEQUENCED ON: 2/22/2010
EXPECTED MW: 52kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAVSTEEAV KASLFPMEDF EIMEIRKAFI ERITRVREDA SSGDFLDSHI DLSKARMPYQ ESRSVLVIDV GGTSLKISEV VIDLGSDEKG NSPSFHDIVE CSPSFYRYPD TSGVSGQDRI SWNEWVADQV VEFYGRNPPR SKVNASLTFS YPLIQTSVNN AQIKKTTKNF CFREDEATFT TDIVEALNSS LRSRKINVCV NCVLNDSTAT YMAGVLRGYK NIIGIVLGTG TNSSFCVKKG RSNEVVLYNS EWGSTRVPRS MLTEADLAVV VDLEMRKISH NIIDVLAGGC KLGDIVLHKL RNTYPEIYEE YAEREEVLHS MIHSAITGSG NGALAKSPKL RNALMPIVRD FRARGMKILA SLIGAVVEST MDNANTITLV LNGTTFSNAG LRDALGMEVK RMHPDIEINL VFLSNASLEG SALVSLMYSE YPKVECDLAP LAAPVLSFAD QTP
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggctg taagtacgga ggaggctgtt aaggccagct tatttcccat ggaggacttc gagatcatgg agataagaaa agcctttatt gaaaggatca caagggtacg ggaagatgct tctagtggag attttctgga ttcccatatc gatcttagta aagcaaggat gccatatcag gagagccgct cagtactagt tatagatgtc ggaggaacaa gtctgaagat aagtgaggtt gtcatcgacc ttggttctga cgaaaaggga aactctccgt ctttccacga catagtggaa tgtagcccat ctttctacag atatcctgac acctcgggtg tttcgggcca agacaggata agctggaacg agtgggttgc agaccaggtt gttgaattct atggcagaaa ccctccaaga agtaaggtta atgcatctct gacattctcc tatcctctta tccagacatc tgttaataac gcacaaataa aaaagacaac aaagaacttc tgcttcagag aggacgaggc cacgtttacc acggacatag tcgaggccct caactcctcg ctgaggagca ggaagatcaa cgtctgtgtc aactgcgtct taaatgactc cacagcaaca tacatggcag gagtgctcag gggatataag aacataatcg ggattgttct tgggacaggg acaaactcgt ccttctgtgt gaagaagggc agaagcaatg aggtggtact atacaactcc gaatggggat ccacaagagt tccaaggagc atgttaacag aagcagacct tgccgttgtt gtggacctgg aaatgagaaa gatttcgcat aacatcattg acgtcttagc aggcggctgt aaacttggtg acatcgttct tcacaaactt agaaatacgt atcctgaaat ttatgaggaa tacgcagaga gggaagaggt gctccattcg atgatccact cagccatcac cggatctgga aacggcgcac tggctaaaag ccccaaacta cgcaacgcac tgatgcccat agttcgtgac tttagggcca ggggaatgaa gatattggca tccttaatag gtgctgtggt cgaatccacc atggataatg caaacacaat cacgctcgtt ctgaacggaa caaccttctc gaatgcaggc ctcagagatg ctcttggaat ggaggtaaaa aggatgcatc cggatatcga gatcaacctt gtcttcctta gtaatgcctc tctggaagga tctgcacttg tatcccttat gtattccgag taccccaagg tagaatgtga cttggcacca cttgccgctc cagtcctttc atttgccgat cagactcctt a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAVSTEEAV KASLFPMEDF EIMEIRKAFI ERITRVREDA SSGDFLDSHI DLSKARMPYQ ESRSVLVIDV GGTSLKISEV VIDLGSDEKG NSPSFHDIVE CSPSFYRYPD TSGVSGQDRI SWNEWVADQV VEFYGRNPPR SKVNASLTFS YPLIQTSVNN AQIKKTTKNF CFREDEATFT TDIVEALNSS LRSRKINVCV NCVLNDSTAT YMAGVLRGYK NIIGIVLGTG TNSSFCVKKG RSNEVVLYNS EWGSTRVPRS MLTEADLAVV VDLEMRKISH NIIDVLAGGC KLGDIVLHKL RNTYPEIYEE YAEREEVLHS MIHSAITGSG NGALAKSPKL RNALMPIVRD FRARGMKILA SLIGAVVEST MDNANTITLV LNGTTFSNAG LRDALGMEVK RMHPDIEINL VFLSNASLEG SALVSLMYSE YPKVECDLAP LAAPVLSFAD QTP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctgtaagt acggaggagg ctgttaaggc cagcttattt cccatggagg acttcgagat catggagata agaaaagcct ttattgaaag gatcacaagg gtacgggaag atgcttctag tggagatttt ctggattccc atatcgatct tagtaaagca aggatgccat atcaggagag ccgctcagta ctagttatag atgtcggagg aacaagtctg aagataagtg aggttgtcat cgaccttggt tctgacgaaa agggaaactc tccgtctttc cacgacatag tggaatgtag cccatctttc tacagatatc ctgacacctc gggtgtttcg ggccaagaca ggataagctg gaacgagtgg gttgcagacc aggttgttga attctatggc agaaaccctc caagaagtaa ggttaatgca tctctgacat tctcctatcc tcttatccag acatctgtta ataacgcaca aataaaaaag acaacaaaga acttctgctt cagagaggac gaggccacgt ttaccacgga catagtcgag gccctcaact cctcgctgag gagcaggaag atcaacgtct gtgtcaactg cgtcttaaat gactccacag caacatacat ggcaggagtg ctcaggggat ataagaacat aatcgggatt gttcttggga cagggacaaa ctcgtccttc tgtgtgaaga agggcagaag caatgaggtg gtactataca actccgaatg gggatccaca agagttccaa ggagcatgtt aacagaagca gaccttgccg ttgttgtgga cctggaaatg agaaagattt cgcataacat cattgacgtc ttagcaggcg gctgtaaact tggtgacatc gttcttcaca aacttagaaa tacgtatcct gaaatttatg aggaatacgc agagagggaa gaggtgctcc attcgatgat ccactcagcc atcaccggat ctggaaacgg cgcactggct aaaagcccca aactacgcaa cgcactgatg cccatagttc gtgactttag ggccagggga atgaagatat tggcatcctt aataggtgct gtggtcgaat ccaccatgga taatgcaaac acaatcacgc tcgttctgaa cggaacaacc ttctcgaatg caggcctcag agatgctctt ggaatggagg taaaaaggat gcatccggat atcgagatca accttgtctt ccttagtaat gcctctctgg aaggatctgc acttgtatcc cttatgtatt ccgagtaccc caaggtagaa tgtgacttgg caccacttgc cgctccagtc ctttcatttg ccgatcagac tcctaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for EncuA.10272.a.A2.PS00636
PURIFICATION DATe: 6/10/2010
CONCENTRATION: 15.4mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 12
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAVSTEEAV KASLFPMEDF EIMEIRKAFI ERITRVREDA SSGDFLDSHI DLSKARMPYQ ESRSVLVIDV GGTSLKISEV VIDLGSDEKG NSPSFHDIVE CSPSFYRYPD TSGVSGQDRI SWNEWVADQV VEFYGRNPPR SKVNASLTFS YPLIQTSVNN AQIKKTTKNF CFREDEATFT TDIVEALNSS LRSRKINVCV NCVLNDSTAT YMAGVLRGYK NIIGIVLGTG TNSSFCVKKG RSNEVVLYNS EWGSTRVPRS MLTEADLAVV VDLEMRKISH NIIDVLAGGC KLGDIVLHKL RNTYPEIYEE YAEREEVLHS MIHSAITGSG NGALAKSPKL RNALMPIVRD FRARGMKILA SLIGAVVEST MDNANTITLV LNGTTFSNAG LRDALGMEVK RMHPDIEINL VFLSNASLEG SALVSLMYSE YPKVECDLAP LAAPVLSFAD QTP
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggctg taagtacgga ggaggctgtt aaggccagct tatttcccat ggaggacttc gagatcatgg agataagaaa agcctttatt gaaaggatca caagggtacg ggaagatgct tctagtggag attttctgga ttcccatatc gatcttagta aagcaaggat gccatatcag gagagccgct cagtactagt tatagatgtc ggaggaacaa gtctgaagat aagtgaggtt gtcatcgacc ttggttctga cgaaaaggga aactctccgt ctttccacga catagtggaa tgtagcccat ctttctacag atatcctgac acctcgggtg tttcgggcca agacaggata agctggaacg agtgggttgc agaccaggtt gttgaattct atggcagaaa ccctccaaga agtaaggtta atgcatctct gacattctcc tatcctctta tccagacatc tgttaataac gcacaaataa aaaagacaac aaagaacttc tgcttcagag aggacgaggc cacgtttacc acggacatag tcgaggccct caactcctcg ctgaggagca ggaagatcaa cgtctgtgtc aactgcgtct taaatgactc cacagcaaca tacatggcag gagtgctcag gggatataag aacataatcg ggattgttct tgggacaggg acaaactcgt ccttctgtgt gaagaagggc agaagcaatg aggtggtact atacaactcc gaatggggat ccacaagagt tccaaggagc atgttaacag aagcagacct tgccgttgtt gtggacctgg aaatgagaaa gatttcgcat aacatcattg acgtcttagc aggcggctgt aaacttggtg acatcgttct tcacaaactt agaaatacgt atcctgaaat ttatgaggaa tacgcagaga gggaagaggt gctccattcg atgatccact cagccatcac cggatctgga aacggcgcac tggctaaaag ccccaaacta cgcaacgcac tgatgcccat agttcgtgac tttagggcca ggggaatgaa gatattggca tccttaatag gtgctgtggt cgaatccacc atggataatg caaacacaat cacgctcgtt ctgaacggaa caaccttctc gaatgcaggc ctcagagatg ctcttggaat ggaggtaaaa aggatgcatc cggatatcga gatcaacctt gtcttcctta gtaatgcctc tctggaagga tctgcacttg tatcccttat gtattccgag taccccaagg tagaatgtga cttggcacca cttgccgctc cagtcctttc atttgccgat cagactcctt a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAVSTEEAV KASLFPMEDF EIMEIRKAFI ERITRVREDA SSGDFLDSHI DLSKARMPYQ ESRSVLVIDV GGTSLKISEV VIDLGSDEKG NSPSFHDIVE CSPSFYRYPD TSGVSGQDRI SWNEWVADQV VEFYGRNPPR SKVNASLTFS YPLIQTSVNN AQIKKTTKNF CFREDEATFT TDIVEALNSS LRSRKINVCV NCVLNDSTAT YMAGVLRGYK NIIGIVLGTG TNSSFCVKKG RSNEVVLYNS EWGSTRVPRS MLTEADLAVV VDLEMRKISH NIIDVLAGGC KLGDIVLHKL RNTYPEIYEE YAEREEVLHS MIHSAITGSG NGALAKSPKL RNALMPIVRD FRARGMKILA SLIGAVVEST MDNANTITLV LNGTTFSNAG LRDALGMEVK RMHPDIEINL VFLSNASLEG SALVSLMYSE YPKVECDLAP LAAPVLSFAD QTP
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCTGTAAGT ACGGAGGAGG CTGTTAAGGC CAGCTTATTT CCCATGGAGG ACTTCGAGAT CATGGAGATA AGAAAAGCCT TTATTGAAAG GATCACAAGG GTACGGGAAG ATGCTTCTAG TGGAGATTTT CTGGATTCCC ATATCGATCT TAGTAAAGCA AGGATGCCAT ATCAGGAGAG CCGCTCAGTA CTAGTTATAG ATGTCGGAGG AACAAGTCTG AAGATAAGTG AGGTTGTCAT CGACCTTGGT TCTGACGAAA AGGGAAACTC TCCGTCTTTC CACGACATAG TGGAATGTAG CCCATCTTTC TACAGATATC CTGACACCTC GGGTGTTTCG GGCCAAGACA GGATAAGCTG GAACGAGTGG GTTGCAGACC AGGTTGTTGA ATTCTATGGC AGAAACCCTC CAAGAAGTAA GGTTAATGCA TCTCTGACAT TCTCCTATCC TCTTATCCAG ACATCTGTTA ATAACGCACA AATAAAAAAG ACAACAAAGA ACTTCTGCTT CAGAGAGGAC GAGGCCACGT TTACCACGGA CATAGTCGAG GCCCTCAACT CCTCGCTGAG GAGCAGGAAG ATCAACGTCT GTGTCAACTG CGTCTTAAAT GACTCCACAG CAACATACAT GGCAGGAGTG CTCAGGGGAT ATAAGAACAT AATCGGGATT GTTCTTGGGA CAGGGACAAA CTCGTCCTTC TGTGTGAAGA AGGGCAGAAG CAATGAGGTG GTACTATACA ACTCCGAATG GGGATCCACA AGAGTTCCAA GGAGCATGTT AACAGAAGCA GACCTTGCCG TTGTTGTGGA CCTGGAAATG AGAAAGATTT CGCATAACAT CATTGACGTC TTAGCAGGCG GCTGTAAACT TGGTGACATC GTTCTTCACA AACTTAGAAA TACGTATCCT GAAATTTATG AGGAATACGC AGAGAGGGAA GAGGTGCTCC ATTCGATGAT CCACTCAGCC ATCACCGGAT CTGGAAACGG CGCACTGGCT AAAAGCCCCA AACTACGCAA CGCACTGATG CCCATAGTTC GTGACTTTAG GGCCAGGGGA ATGAAGATAT TGGCATCCTT AATAGGTGCT GTGGTCGAAT CCACCATGGA TAATGCAAAC ACAATCACGC TCGTTCTGAA CGGAACAACC TTCTCGAATG CAGGCCTCAG AGATGCTCTT GGAATGGAGG TAAAAAGGAT GCATCCGGAT ATCGAGATCA ACCTTGTCTT CCTTAGTAAT GCCTCTCTGG AAGGATCTGC ACTTGTATCC CTTATGTATT CCGAGTACCC CAAGGTAGAA TGTGACTTGG CACCACTTGC CGCTCCAGTC CTTTCATTTG CCGATCAGAC TCCTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA