EncuA.01641.a

Probable cAMP-dependent protein kinase catalytic subunit

CENTER ID: EncuA.01641.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.01641.a.A1.GE27885 Full length( EncuA.01641.a ) 1 322
EncuA.01641.a.A1.GE28372 Full length( EncuA.01641.a ) 1 322
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU07_0520
RefSeq: NP_585980.1
UniProt: Q8SRK8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLKIQDFEFV KVVGVGAFGK VHLVRLKSSP TSVFAMKMLD FGEILRQRLA DQLENEISIL KRIYGCPFVA KLYSTDFHGG KVGLIMEYVG GGELFYWLKK CGRFDEQMAR FYAAEIISAL RFIHGRGILY RDLKPENILI TSTGHIKLID FGFSVYESEN IYMISGTPEY MSPEKLRSED DGRASDYWGL GVMIYEMLCG DPPFYDSSAD AIYHKILESN VVFPHYVSPV ARCLITGLLD KNRATRLGTK GICEIMGHPF FKGIDWHEVE SRRIEPPFIP NPNTVLSSLA SSGELKGTDD AETVVLKPYK HIKHLYKVSK GL
NT Sequence
ATGTTGAAGA TCCAGGACTT CGAATTTGTA AAAGTCGTGG GAGTGGGGGC CTTTGGCAAG GTGCATCTGG TGAGGCTCAA GAGCAGTCCC ACGTCCGTGT TTGCAATGAA AATGCTCGAC TTCGGAGAGA TTCTCAGGCA AAGGCTGGCG GATCAGCTTG AAAACGAGAT CTCCATTCTG AAGCGGATCT ATGGGTGCCC GTTCGTTGCA AAGCTGTACT CGACAGACTT CCACGGTGGA AAGGTGGGAC TGATTATGGA GTATGTCGGA GGAGGAGAGT TGTTTTACTG GCTCAAAAAG TGCGGAAGAT TTGATGAGCA GATGGCCCGG TTTTATGCAG CAGAGATCAT TTCCGCCTTG CGATTCATAC ATGGAAGGGG CATTCTATAC AGAGATCTGA AGCCCGAGAA CATACTGATC ACATCCACAG GACACATAAA GCTGATTGAC TTTGGGTTTT CTGTCTACGA AAGCGAAAAC ATATACATGA TTTCAGGGAC TCCAGAATAT ATGTCTCCAG AAAAGCTGAG GAGCGAGGAT GACGGGCGTG CAAGCGACTA TTGGGGACTG GGGGTTATGA TCTACGAGAT GCTGTGTGGA GACCCTCCGT TCTATGACTC AAGTGCAGAT GCGATTTACC ACAAGATACT GGAGTCCAAT GTTGTGTTTC CCCACTATGT AAGCCCGGTT GCAAGGTGTC TAATAACAGG GCTGTTAGAC AAGAACAGGG CTACCAGGCT GGGGACCAAA GGAATATGCG AGATAATGGG GCATCCTTTC TTCAAGGGGA TAGACTGGCA CGAGGTTGAG AGCAGGAGGA TTGAGCCCCC GTTCATTCCC AATCCAAACA CTGTTCTTAG TTCTTTGGCT AGCAGCGGTG AACTCAAAGG CACGGATGAT GCAGAGACGG TTGTTTTAAA GCCGTACAAG CATATTAAGC ATCTGTACAA GGTCTCGAAG GGACTG
Details for EncuA.01641.a.A1.GE27885
HARVESTED ON: 11/3/2009
SEQUENCED ON: 11/10/2009
EXPECTED MW: 38kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMLKIQDF EFVKVVGVGA FGKVHLVRLK SSPTSVFAMK MLDFGEILRQ RLADQLENEI SILKRIYGCP FVAKLYSTDF HGGKVGLIME YVGGGELFYW LKKCGRFDEQ MARFYAAEII SALRFIHGRG ILYRDLKPEN ILITSTGHIK LIDFGFSVYE SENIYMISGT PEYMSPEKLR SEDDGRASDY WGLGVMIYEM LCGDPPFYDS SADAIYHKIL ESNVVFPHYV SPVARCLITG LLDKNRATRL GTKGICEIMG HPFFKGIDWH EVESRRIEPP FIPNPNTVLS SLASSGELKG TDDAETVVLK PYKHIKHLYK VSKGL
Validated NT Sequence
tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgaagatc caggacttcg aatttgtaaa agtcgtggga gtgggggcct ttggcaaggt gcatctggtg aggctcaaga gcagtcccac gtccgtgttt gcaatgaaaa tgctcgactt cggagagatt ctcaggcaaa ggctggcgga tcagcttgaa aacgagatct ccattctgaa gcggatctat gggtgcccgt tcgttgcaaa gctgtactcg acagacttcc acggtggaaa ggtgggactg attatggagt atgtcggagg aggagagttg ttttactggc tcaaaaagtg cggaagattt gatgagcaga tggcccggtt ttatgcagca gagatcattt ccgccttgcg attcatacat ggaaggggca ttctatacag agatctgaag cccgagaaca tactgatcac atccacagga cacataaagc tgattgactt tgggttttct gtctacgaaa gcgaaaacat atacatgatt tcagggactc cagaatatat gtctccagaa aagctgagga gcgaggatga cgggcgtgca agcgactatt ggggactggg ggttatgatc tacgagatgc tgtgtggaga ccctccgttc tatgactcaa gtgcagatgc gatttaccac aagatactgg agtccaatgt tgtgtttccc cactatgtaa gcccggttgc aaggtgtcta ataacagggc tgttagacaa gaacagggct accaggctgg ggaccaaagg aatatgcgag ataatggggc atcctttctt caaggggata gactggcacg aggttgagag caggaggatt gagcccccgt tcattcccaa tccaaacact gttcttagtt ctttggctag cagcggtgaa ctcaaaggca cggatgatgc agagacggtt gttttaaagc cgtacaagca tattaagcat ctgtacaagg tctcgaaggg actgtaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLKIQDFEF VKVVGVGAFG KVHLVRLKSS PTSVFAMKML DFGEILRQRL ADQLENEISI LKRIYGCPFV AKLYSTDFHG GKVGLIMEYV GGGELFYWLK KCGRFDEQMA RFYAAEIISA LRFIHGRGIL YRDLKPENIL ITSTGHIKLI DFGFSVYESE NIYMISGTPE YMSPEKLRSE DDGRASDYWG LGVMIYEMLC GDPPFYDSSA DAIYHKILES NVVFPHYVSP VARCLITGLL DKNRATRLGT KGICEIMGHP FFKGIDWHEV ESRRIEPPFI PNPNTVLSSL ASSGELKGTD DAETVVLKPY KHIKHLYKVS KGL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgaagatc caggacttcg aatttgtaaa agtcgtggga gtgggggcct ttggcaaggt gcatctggtg aggctcaaga gcagtcccac gtccgtgttt gcaatgaaaa tgctcgactt cggagagatt ctcaggcaaa ggctggcgga tcagcttgaa aacgagatct ccattctgaa gcggatctat gggtgcccgt tcgttgcaaa gctgtactcg acagacttcc acggtggaaa ggtgggactg attatggagt atgtcggagg aggagagttg ttttactggc tcaaaaagtg cggaagattt gatgagcaga tggcccggtt ttatgcagca gagatcattt ccgccttgcg attcatacat ggaaggggca ttctatacag agatctgaag cccgagaaca tactgatcac atccacagga cacataaagc tgattgactt tgggttttct gtctacgaaa gcgaaaacat atacatgatt tcagggactc cagaatatat gtctccagaa aagctgagga gcgaggatga cgggcgtgca agcgactatt ggggactggg ggttatgatc tacgagatgc tgtgtggaga ccctccgttc tatgactcaa gtgcagatgc gatttaccac aagatactgg agtccaatgt tgtgtttccc cactatgtaa gcccggttgc aaggtgtcta ataacagggc tgttagacaa gaacagggct accaggctgg ggaccaaagg aatatgcgag ataatggggc atcctttctt caaggggata gactggcacg aggttgagag caggaggatt gagcccccgt tcattcccaa tccaaacact gttcttagtt ctttggctag cagcggtgaa ctcaaaggca cggatgatgc agagacggtt gttttaaagc cgtacaagca tattaagcat ctgtacaagg tctcgaaggg actgaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for EncuA.01641.a.A1.GE28372
HARVESTED ON: 1/5/2010
SEQUENCED ON: 1/15/2010
EXPECTED MW: 38kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMLKIQDF EFVKVVGVGA FGKVHLVRLK SSPTSVFAMK MLDFGEILRQ RLADQLENEI SILKRIYGCP FVAKLYSTDF HGGKVGLIME YVGGGELFYW LKKCGRFDEQ MARFYAAEII SALRFIHGRG ILYRDLKPEN ILITSTGHIK LIDFGFSVYE SENIYMISGT PEYMSPEKLR SEDDGRASDY WGLGVMIYEM LCGDPPFYDS SADAIYHKIL ESNVVFPHYV SPVARCLITG LLDKNRATRL GTKGICEIMG HPFFKGIDWH EVESRRIEPP FIPNPNTVLS SLASSGELKG TDDAETVVLK PYKHIKHLYK VSKGL
Validated NT Sequence
tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgaagatc caggacttcg aatttgtaaa agtcgtggga gtgggggcct ttggcaaggt gcatctggtg aggctcaaga gcagtcccac gtccgtgttt gcaatgaaaa tgctcgactt cggagagatt ctcaggcaaa ggctggcgga tcagcttgaa aacgagatct ccattctgaa gcggatctat gggtgcccgt tcgttgcaaa gctgtactcg acagacttcc acggtggaaa ggtgggactg attatggagt atgtcggagg aggagagttg ttttactggc tcaaaaagtg cggaagattt gatgagcaga tggcccggtt ttatgcagca gagatcattt ccgccttgcg attcatacat ggaaggggca ttctatacag agatctgaag cccgagaaca tactgatcac atccacagga cacataaagc tgattgactt tgggttttct gtctacgaaa gcgaaaacat atacatgatt tcagggactc cagaatatat gtctccagaa aagctgagga gcgaggatga cgggcgtgca agcgactatt ggggactggg ggttatgatc tacgagatgc tgtgtggaga ccctccgttc tatgactcaa gtgcagatgc gatttaccac aagatactgg agtccaatgt tgtgtttccc cactatgtaa gcccggttgc aaggtgtcta ataacagggc tgttagacaa gaacagggct accaggctgg ggaccaaagg aatatgcgag ataatggggc atcctttctt caaggggata gactggcacg aggttgagag caggaggatt gagcccccgt tcattcccaa tccaaacact gttcttagtt ctttggctag cagcggtgaa ctcaaaggca cggatgatgc agagacggtt gttttaaagc cgtacaagca tattaagcat ctgtacaagg tctcgaaggg actgtaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLKIQDFEF VKVVGVGAFG KVHLVRLKSS PTSVFAMKML DFGEILRQRL ADQLENEISI LKRIYGCPFV AKLYSTDFHG GKVGLIMEYV GGGELFYWLK KCGRFDEQMA RFYAAEIISA LRFIHGRGIL YRDLKPENIL ITSTGHIKLI DFGFSVYESE NIYMISGTPE YMSPEKLRSE DDGRASDYWG LGVMIYEMLC GDPPFYDSSA DAIYHKILES NVVFPHYVSP VARCLITGLL DKNRATRLGT KGICEIMGHP FFKGIDWHEV ESRRIEPPFI PNPNTVLSSL ASSGELKGTD DAETVVLKPY KHIKHLYKVS KGL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgaagatc caggacttcg aatttgtaaa agtcgtggga gtgggggcct ttggcaaggt gcatctggtg aggctcaaga gcagtcccac gtccgtgttt gcaatgaaaa tgctcgactt cggagagatt ctcaggcaaa ggctggcgga tcagcttgaa aacgagatct ccattctgaa gcggatctat gggtgcccgt tcgttgcaaa gctgtactcg acagacttcc acggtggaaa ggtgggactg attatggagt atgtcggagg aggagagttg ttttactggc tcaaaaagtg cggaagattt gatgagcaga tggcccggtt ttatgcagca gagatcattt ccgccttgcg attcatacat ggaaggggca ttctatacag agatctgaag cccgagaaca tactgatcac atccacagga cacataaagc tgattgactt tgggttttct gtctacgaaa gcgaaaacat atacatgatt tcagggactc cagaatatat gtctccagaa aagctgagga gcgaggatga cgggcgtgca agcgactatt ggggactggg ggttatgatc tacgagatgc tgtgtggaga ccctccgttc tatgactcaa gtgcagatgc gatttaccac aagatactgg agtccaatgt tgtgtttccc cactatgtaa gcccggttgc aaggtgtcta ataacagggc tgttagacaa gaacagggct accaggctgg ggaccaaagg aatatgcgag ataatggggc atcctttctt caaggggata gactggcacg aggttgagag caggaggatt gagcccccgt tcattcccaa tccaaacact gttcttagtt ctttggctag cagcggtgaa ctcaaaggca cggatgatgc agagacggtt gttttaaagc cgtacaagca tattaagcat ctgtacaagg tctcgaaggg actgaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa