EncuA.01574.a

Actin

CENTER ID: EncuA.01574.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.01574.a.A1.GE27806 Full length( EncuA.01574.a ) 1 375
EncuA.01574.a.A1.GE28369 Full length( EncuA.01574.a ) 1 375
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU01_0460
OrthoMCL: OG5_126595
RefSeq: XP_965880.1
UniProt: Q8SWN8
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSEIVQALVI DIGSGVVKSG FAGDDAPRAV FPSIVGFPKH KGVMVGMGQK DAYVGDEAQT KRGILHIKYP IEHGIVNNWD DMEKIWHHTF YNELRVAPEE HPVLLTEAPL NPKANREKIT QIMFETFNVP SFYISIQAVL SLYASGRTTG IVFDSGDGVS HVVPIYEGYS LPYAINRIDL AGRDLTDYLQ LILTESGNSF TTTAEREIVR DIKEKLCYVS LNYEEDMRNT EHLASITKTY EMPDGQVISI GNERFRAPEL LFQPKLRGLE LKGIHQNIYD SIMKCDVDIR KELYGNIVLS GGTTMYPGLA ERILNEIKAL APPVIKIGVV APPERKYSVW IGGSILASLS TFQQMWVSKA EYQEHGPSIV HRKCF
NT Sequence
ATGTCCGAAA TAGTTCAGGC ACTTGTTATT GATATTGGAT CTGGAGTAGT GAAGTCTGGG TTTGCCGGAG ACGACGCACC TAGGGCTGTA TTTCCTAGCA TAGTGGGGTT TCCCAAGCAC AAGGGAGTGA TGGTTGGAAT GGGACAGAAA GATGCATATG TGGGGGATGA GGCACAAACC AAGAGAGGAA TTCTGCACAT AAAGTATCCG ATTGAGCATG GAATTGTAAA CAACTGGGAC GACATGGAGA AGATATGGCA CCACACATTC TACAACGAGC TGAGGGTTGC GCCTGAGGAG CATCCTGTGC TTCTTACGGA AGCGCCACTT AACCCAAAGG CCAACAGGGA AAAGATAACT CAGATTATGT TTGAGACCTT CAATGTGCCA TCATTCTACA TTTCGATCCA GGCAGTGCTG TCTCTCTATG CAAGTGGGAG GACGACAGGG ATAGTGTTTG ACTCTGGTGA CGGGGTGTCT CATGTTGTTC CGATCTACGA GGGATACTCC CTTCCATACG CAATCAACAG GATTGACCTT GCCGGAAGGG ATCTGACCGA CTATCTTCAG CTGATTCTTA CCGAGAGTGG AAACTCGTTC ACCACTACTG CCGAAAGAGA GATAGTCAGG GACATCAAGG AAAAGCTCTG CTATGTATCG CTGAACTATG AGGAAGACAT GAGAAACACC GAGCATCTGG CTTCGATCAC AAAGACTTAT GAAATGCCTG ATGGACAGGT GATTTCGATA GGTAATGAGA GATTCAGGGC ACCTGAGCTG CTATTCCAGC CAAAGCTCCG GGGTCTTGAG CTGAAGGGAA TCCATCAGAA CATCTATGAC TCGATCATGA AGTGCGACGT TGATATCAGG AAGGAGCTCT ACGGAAACAT TGTTCTCTCT GGAGGTACCA CGATGTATCC CGGATTGGCC GAGAGGATTC TTAATGAGAT TAAGGCTCTG GCACCTCCAG TGATCAAGAT TGGAGTGGTT GCGCCGCCGG AGAGGAAGTA CAGCGTGTGG ATTGGAGGGT CCATCCTGGC CTCTCTCTCA ACCTTCCAGC AGATGTGGGT TTCCAAGGCT GAGTACCAGG AGCACGGGCC ATCGATTGTT CACAGAAAGT GCTTC
Details for EncuA.01574.a.A1.GE27806
HARVESTED ON: 11/3/2009
SEQUENCED ON: 11/10/2009
EXPECTED MW: 44kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSEIVQALV IDIGSGVVKS GFAGDDAPRA VFPSIVGFPK HKGVMVGMGQ KDAYVGDEAQ TKRGILHIKY PIEHGIVNNW DDMEKIWHHT FYNELRVAPE EHPVLLTEAP LNPKANREKI TQIMFETFNV PSFYISIQAV LSLYASGRTT GIVFDSGDGV SHVVPIYEGY SLPYAINRID LAGRDLTDYL QLILTESGNS FTTTAEREIV RDIKEKLCYV SLNYEEDMRN TEHLASITKT YEMPDGQVIS IGNERFRAPE LLFQPKLRGL ELKGIHQNIY DSIMKCDVDI RKELYGNIVL SGGTTMYPGL AERILNEIKA LAPPVIKIGV VAPPERKYSV WIGGSILASL STFQQMWVSK AEYQEHGPSI VHRKCF
Validated NT Sequence
ttagaagcac tttctgtgaa caatcgatgg cccgtgctcc tggtactcag ccttggaaac ccacatctgc tggaaggttg agagagaggc caggatggac cctccaatcc acacgctgta cttcctctcc ggcggcgcaa ccactccaat cttgatcact ggaggtgcca gagccttaat ctcattaaga atcctctcgg ccaatccggg atacatcgtg gtacctccag agagaacaat gtttccgtag agctccttcc tgatatcaac gtcgcacttc atgatcgagt catagatgtt ctgatggatt cccttcagct caagaccccg gagctttggc tggaatagca gctcaggtgc cctgaatctc tcattaccta tcgaaatcac ctgtccatca ggcatttcat aagtctttgt gatcgaagcc agatgctcgg tgtttctcat gtcttcctca tagttcagcg atacatagca gagcttttcc ttgatgtccc tgactatctc tctttcggca gtagtggtga acgagtttcc actctcggta agaatcagct gaagatagtc ggtcagatcc cttccggcaa ggtcaatcct gttgattgcg tatggaaggg agtatccctc gtagatcgga acaacatgag acaccccgtc accagagtca aacactatcc ctgtcgtcct cccacttgca tagagagaca gcactgcctg gatcgaaatg tagaatgatg gcacattgaa ggtctcaaac ataatctgag ttatcttttc cctgttggcc tttgggttaa gtggcgcttc cgtaagaagc acaggatgct cctcaggcgc aaccctcagc tcgttgtaga atgtgtggtg ccatatcttc tccatgtcgt cccagttgtt tacaattcca tgctcaatcg gatactttat gtgcagaatt cctctcttgg tttgtgcctc atcccccaca tatgcatctt tctgtcccat tccaaccatc actcccttgt gcttgggaaa ccccactatg ctaggaaata cagccctagg tgcgtcgtct ccggcaaacc cagacttcac tactccagat ccaatatcaa taacaagtgc ctgaactatt tcggacatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSEIVQALV IDIGSGVVKS GFAGDDAPRA VFPSIVGFPK HKGVMVGMGQ KDAYVGDEAQ TKRGILHIKY PIEHGIVNNW DDMEKIWHHT FYNELRVAPE EHPVLLTEAP LNPKANREKI TQIMFETFNV PSFYISIQAV LSLYASGRTT GIVFDSGDGV SHVVPIYEGY SLPYAINRID LAGRDLTDYL QLILTESGNS FTTTAEREIV RDIKEKLCYV SLNYEEDMRN TEHLASITKT YEMPDGQVIS IGNERFRAPE LLFQPKLRGL ELKGIHQNIY DSIMKCDVDI RKELYGNIVL SGGTTMYPGL AERILNEIKA LAPPVIKIGV VAPPERKYSV WIGGSILASL STFQQMWVSK AEYQEHGPSI VHRKCF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtccgaaata gttcaggcac ttgttattga tattggatct ggagtagtga agtctgggtt tgccggagac gacgcaccta gggctgtatt tcctagcata gtggggtttc ccaagcacaa gggagtgatg gttggaatgg gacagaaaga tgcatatgtg ggggatgagg cacaaaccaa gagaggaatt ctgcacataa agtatccgat tgagcatgga attgtaaaca actgggacga catggagaag atatggcacc acacattcta caacgagctg agggttgcgc ctgaggagca tcctgtgctt cttacggaag cgccacttaa cccaaaggcc aacagggaaa agataactca gattatgttt gagaccttca atgtgccatc attctacatt tcgatccagg cagtgctgtc tctctatgca agtgggagga cgacagggat agtgtttgac tctggtgacg gggtgtctca tgttgttccg atctacgagg gatactccct tccatacgca atcaacagga ttgaccttgc cggaagggat ctgaccgact atcttcagct gattcttacc gagagtggaa actcgttcac cactactgcc gaaagagaga tagtcaggga catcaaggaa aagctctgct atgtatcgct gaactatgag gaagacatga gaaacaccga gcatctggct tcgatcacaa agacttatga aatgcctgat ggacaggtga tttcgatagg taatgagaga ttcagggcac ctgagctgct attccagcca aagctccggg gtcttgagct gaagggaatc catcagaaca tctatgactc gatcatgaag tgcgacgttg atatcaggaa ggagctctac ggaaacattg ttctctctgg aggtaccacg atgtatcccg gattggccga gaggattctt aatgagatta aggctctggc acctccagtg atcaagattg gagtggttgc gccgccggag aggaagtaca gcgtgtggat tggagggtcc atcctggcct ctctctcaac cttccagcag atgtgggttt ccaaggctga gtaccaggag cacgggccat cgattgttca cagaaagtgc ttcaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for EncuA.01574.a.A1.GE28369
HARVESTED ON: 1/5/2010
SEQUENCED ON: 1/15/2010
EXPECTED MW: 44kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSEIVQALV IDIGSGVVKS GFAGDDAPRA VFPSIVGFPK HKGVMVGMGQ KDAYVGDEAQ TKRGILHIKY PIEHGIVNNW DDMEKIWHHT FYNELRVAPE EHPVLLTEAP LNPKANREKI TQIMFETFNV PSFYISIQAV LSLYASGRTT GIVFDSGDGV SHVVPIYEGY SLPYAINRID LAGRDLTDYL QLILTESGNS FTTTAEREIV RDIKEKLCYV SLNYEEDMRN TEHLASITKT YEMPDGQVIS IGNERFRAPE LLFQPKLRGL ELKGIHQNIY DSIMKCDVDI RKELYGNIVL SGGTTMYPGL AERILNEIKA LAPPVIKIGV VAPPERKYSV WIGGSILASL STFQQMWVSK AEYQEHGPSI VHRKCF
Validated NT Sequence
ttagaagcac tttctgtgaa caatcgatgg cccgtgctcc tggtactcag ccttggaaac ccacatctgc tggaaggttg agagagaggc caggatggac cctccaatcc acacgctgta cttcctctcc ggcggcgcaa ccactccaat cttgatcact ggaggtgcca gagccttaat ctcattaaga atcctctcgg ccaatccggg atacatcgtg gtacctccag agagaacaat gtttccgtag agctccttcc tgatatcaac gtcgcacttc atgatcgagt catagatgtt ctgatggatt cccttcagct caagaccccg gagctttggc tggaatagca gctcaggtgc cctgaatctc tcattaccta tcgaaatcac ctgtccatca ggcatttcat aagtctttgt gatcgaagcc agatgctcgg tgtttctcat gtcttcctca tagttcagcg atacatagca gagcttttcc ttgatgtccc tgactatctc tctttcggca gtagtggtga acgagtttcc actctcggta agaatcagct gaagatagtc ggtcagatcc cttccggcaa ggtcaatcct gttgattgcg tatggaaggg agtatccctc gtagatcgga acaacatgag acaccccgtc accagagtca aacactatcc ctgtcgtcct cccacttgca tagagagaca gcactgcctg gatcgaaatg tagaatgatg gcacattgaa ggtctcaaac ataatctgag ttatcttttc cctgttggcc tttgggttaa gtggcgcttc cgtaagaagc acaggatgct cctcaggcgc aaccctcagc tcgttgtaga atgtgtggtg ccatatcttc tccatgtcgt cccagttgtt tacaattcca tgctcaatcg gatactttat gtgcagaatt cctctcttgg tttgtgcctc atcccccaca tatgcatctt tctgtcccat tccaaccatc actcccttgt gcttgggaaa ccccactatg ctaggaaata cagccctagg tgcgtcgtct ccggcaaacc cagacttcac tactccagat ccaatatcaa taacaagtgc ctgaactatt tcggacatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSEIVQALV IDIGSGVVKS GFAGDDAPRA VFPSIVGFPK HKGVMVGMGQ KDAYVGDEAQ TKRGILHIKY PIEHGIVNNW DDMEKIWHHT FYNELRVAPE EHPVLLTEAP LNPKANREKI TQIMFETFNV PSFYISIQAV LSLYASGRTT GIVFDSGDGV SHVVPIYEGY SLPYAINRID LAGRDLTDYL QLILTESGNS FTTTAEREIV RDIKEKLCYV SLNYEEDMRN TEHLASITKT YEMPDGQVIS IGNERFRAPE LLFQPKLRGL ELKGIHQNIY DSIMKCDVDI RKELYGNIVL SGGTTMYPGL AERILNEIKA LAPPVIKIGV VAPPERKYSV WIGGSILASL STFQQMWVSK AEYQEHGPSI VHRKCF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtccgaaata gttcaggcac ttgttattga tattggatct ggagtagtga agtctgggtt tgccggagac gacgcaccta gggctgtatt tcctagcata gtggggtttc ccaagcacaa gggagtgatg gttggaatgg gacagaaaga tgcatatgtg ggggatgagg cacaaaccaa gagaggaatt ctgcacataa agtatccgat tgagcatgga attgtaaaca actgggacga catggagaag atatggcacc acacattcta caacgagctg agggttgcgc ctgaggagca tcctgtgctt cttacggaag cgccacttaa cccaaaggcc aacagggaaa agataactca gattatgttt gagaccttca atgtgccatc attctacatt tcgatccagg cagtgctgtc tctctatgca agtgggagga cgacagggat agtgtttgac tctggtgacg gggtgtctca tgttgttccg atctacgagg gatactccct tccatacgca atcaacagga ttgaccttgc cggaagggat ctgaccgact atcttcagct gattcttacc gagagtggaa actcgttcac cactactgcc gaaagagaga tagtcaggga catcaaggaa aagctctgct atgtatcgct gaactatgag gaagacatga gaaacaccga gcatctggct tcgatcacaa agacttatga aatgcctgat ggacaggtga tttcgatagg taatgagaga ttcagggcac ctgagctgct attccagcca aagctccggg gtcttgagct gaagggaatc catcagaaca tctatgactc gatcatgaag tgcgacgttg atatcaggaa ggagctctac ggaaacattg ttctctctgg aggtaccacg atgtatcccg gattggccga gaggattctt aatgagatta aggctctggc acctccagtg atcaagattg gagtggttgc gccgccggag aggaagtaca gcgtgtggat tggagggtcc atcctggcct ctctctcaac cttccagcag atgtgggttt ccaaggctga gtaccaggag cacgggccat cgattgttca cagaaagtgc ttcaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa