EncuA.01573.a

SER/THR/TYR PROTEIN KINASE

CENTER ID: EncuA.01573.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.01573.a.A1.GE27854 Full length( EncuA.01573.a ) 1 272
EncuA.01573.a.A1.GE28368 Full length( EncuA.01573.a ) 1 272
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU07_0360
RefSeq: NP_585964.1
UniProt: Q8SRL5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVEYWTLDRF EIGRLLGRGK FGQVWLARER EKGFIVALKI IPIKEIQTVE TARQIRREIE IHSNLKHPNI LRMYGHFHDK DNIYLILEYA GKGEFFKFLS DRGGKFGEKE TSLYIRQVML ALTYMKECNV IHRDIKPENL LLGSDNQLKI ADFGWAVYNA DKRRMTFCGT MEYLAPEMVN NDIHDSGIDL WCLGILTYEF LMGKTPFESK NRNMREAYKK INSLKYTIPE TISSNASDFI SRLLVLSPGD RMELTEALNH PFIVKHHGRS SD
NT Sequence
ATGGTGGAGT ACTGGACTTT GGACAGGTTT GAAATAGGAA GATTGCTTGG GCGTGGAAAG TTCGGGCAGG TATGGCTTGC AAGAGAGAGG GAAAAGGGGT TTATAGTTGC GTTGAAGATT ATTCCAATCA AGGAAATCCA GACGGTGGAG ACGGCAAGGC AGATCCGCAG AGAGATTGAG ATACACTCCA ACCTGAAGCA TCCCAACATT CTGAGGATGT ATGGTCATTT CCACGACAAG GACAATATAT ATCTGATACT GGAATATGCA GGGAAGGGCG AGTTCTTTAA GTTCCTTAGC GACCGGGGAG GCAAGTTCGG GGAGAAAGAG ACATCTCTAT ACATAAGACA GGTGATGCTG GCGCTGACAT ACATGAAGGA GTGTAATGTG ATACACAGAG ACATAAAGCC AGAGAATCTG CTGCTTGGGT CAGACAACCA GCTCAAGATA GCAGATTTTG GATGGGCGGT TTATAATGCT GACAAGAGGC GGATGACCTT CTGTGGTACA ATGGAGTATC TTGCCCCAGA GATGGTTAAC AACGATATAC ACGACTCAGG GATAGACCTG TGGTGCCTGG GGATCCTAAC GTATGAGTTT CTCATGGGAA AAACTCCGTT TGAAAGTAAG AACAGGAACA TGAGGGAGGC CTATAAGAAA ATCAACTCAC TGAAGTATAC CATTCCAGAG ACGATTAGCT CCAACGCTTC AGACTTCATT TCCAGACTTC TTGTGTTGAG TCCTGGCGAC AGAATGGAAC TTACAGAGGC TCTAAATCAT CCGTTTATTG TAAAGCACCA CGGGAGGTCC AGTGAT
Details for EncuA.01573.a.A1.GE27854
HARVESTED ON: 11/3/2009
SEQUENCED ON: 11/10/2009
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVEYWTLDR FEIGRLLGRG KFGQVWLARE REKGFIVALK IIPIKEIQTV ETARQIRREI EIHSNLKHPN ILRMYGHFHD KDNIYLILEY AGKGEFFKFL SDRGGKFGEK ETSLYIRQVM LALTYMKECN VIHRDIKPEN LLLGSDNQLK IADFGWAVYN ADKRRMTFCG TMEYLAPEMV NNDIHDSGID LWCLGILTYE FLMGKTPFES KNRNMREAYK KINSLKYTIP ETISSNASDF ISRLLVLSPG DRMELTEALN HPFIVKHHGR SSD
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgg agtactggac tttggacagg tttgaaatag gaagattgct tgggcgtgga aagttcgggc aggtatggct tgcaagagag agggaaaagg ggtttatagt tgcgttgaag attattccaa tcaaggaaat ccagacggtg gagacggcaa ggcagatccg cagagagatt gagatacact ccaacctgaa gcatcccaac attctgagga tgtatggtca tttccacgac aaggacaata tatatctgat actggaatat gcagggaagg gcgagttctt taagttcctt agcgaccggg gaggcaagtt cggggagaaa gagacatctc tatacataag acaggtgatg ctggcgctga catacatgaa ggagtgtaat gtgatacaca gagacataaa gccagagaat ctgctgcttg ggtcagacaa ccagctcaag atagcagatt ttggatgggc ggtttataat gctgacaaga ggcggatgac cttctgtggt acaatggagt atcttgcccc agagatggtt aacaacgata tacacgactc agggatagac ctgtggtgcc tggggatcct aacgtatgag tttctcatgg gaaaaactcc gtttgaaagt aagaacagga acatgaggga ggcctataag aaaatcaact cactgaagta taccattcca gagacgatta gctccaacgc ttcagacttc atttccagac ttcttgtgtt gagtcctggc gacagaatgg aacttacaga ggctctaaat catccgttta ttgtaaagca ccacgggagg tccagtgatt aataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVEYWTLDR FEIGRLLGRG KFGQVWLARE REKGFIVALK IIPIKEIQTV ETARQIRREI EIHSNLKHPN ILRMYGHFHD KDNIYLILEY AGKGEFFKFL SDRGGKFGEK ETSLYIRQVM LALTYMKECN VIHRDIKPEN LLLGSDNQLK IADFGWAVYN ADKRRMTFCG TMEYLAPEMV NNDIHDSGID LWCLGILTYE FLMGKTPFES KNRNMREAYK KINSLKYTIP ETISSNASDF ISRLLVLSPG DRMELTEALN HPFIVKHHGR SSD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggagtac tggactttgg acaggtttga aataggaaga ttgcttgggc gtggaaagtt cgggcaggta tggcttgcaa gagagaggga aaaggggttt atagttgcgt tgaagattat tccaatcaag gaaatccaga cggtggagac ggcaaggcag atccgcagag agattgagat acactccaac ctgaagcatc ccaacattct gaggatgtat ggtcatttcc acgacaagga caatatatat ctgatactgg aatatgcagg gaagggcgag ttctttaagt tccttagcga ccggggaggc aagttcgggg agaaagagac atctctatac ataagacagg tgatgctggc gctgacatac atgaaggagt gtaatgtgat acacagagac ataaagccag agaatctgct gcttgggtca gacaaccagc tcaagatagc agattttgga tgggcggttt ataatgctga caagaggcgg atgaccttct gtggtacaat ggagtatctt gccccagaga tggttaacaa cgatatacac gactcaggga tagacctgtg gtgcctgggg atcctaacgt atgagtttct catgggaaaa actccgtttg aaagtaagaa caggaacatg agggaggcct ataagaaaat caactcactg aagtatacca ttccagagac gattagctcc aacgcttcag acttcatttc cagacttctt gtgttgagtc ctggcgacag aatggaactt acagaggctc taaatcatcc gtttattgta aagcaccacg ggaggtccag tgataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for EncuA.01573.a.A1.GE28368
HARVESTED ON: 1/5/2010
SEQUENCED ON: 1/15/2010
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVEYWTLDR FEIGRLLGRG KFGQVWLARE REKGFIVALK IIPIKEIQTV ETARQIRREI EIHSNLKHPN ILRMYGHFHD KDNIYLILEY AGKGEFFKFL SDRGGKFGEK ETSLYIRQVM LALTYMKECN VIHRDIKPEN LLLGSDNQLK IADFGWAVYN ADKRRMTFCG TMEYLAPEMV NNDIHDSGID LWCLGILTYE FLMGKTPFES KNRNMREAYK KINSLKYTIP ETISSNASDF ISRLLVLSPG DRMELTEALN HPFIVKHHGR SSD
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgg agtactggac tttggacagg tttgaaatag gaagattgct tgggcgtgga aagttcgggc aggtatggct tgcaagagag agggaaaagg ggtttatagt tgcgttgaag attattccaa tcaaggaaat ccagacggtg gagacggcaa ggcagatccg cagagagatt gagatacact ccaacctgaa gcatcccaac attctgagga tgtatggtca tttccacgac aaggacaata tatatctgat actggaatat gcagggaagg gcgagttctt taagttcctt agcgaccggg gaggcaagtt cggggagaaa gagacatctc tatacataag acaggtgatg ctggcgctga catacatgaa ggagtgtaat gtgatacaca gagacataaa gccagagaat ctgctgcttg ggtcagacaa ccagctcaag atagcagatt ttggatgggc ggtttataat gctgacaaga ggcggatgac cttctgtggt acaatggagt atcttgcccc agagatggtt aacaacgata tacacgactc agggatagac ctgtggtgcc tggggatcct aacgtatgag tttctcatgg gaaaaactcc gtttgaaagt aagaacagga acatgaggga ggcctataag aaaatcaact cactgaagta taccattcca gagacgatta gctccaacgc ttcagacttc atttccagac ttcttgtgtt gagtcctggc gacagaatgg aacttacaga ggctctaaat catccgttta ttgtaaagca ccacgggagg tccagtgatt aataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVEYWTLDR FEIGRLLGRG KFGQVWLARE REKGFIVALK IIPIKEIQTV ETARQIRREI EIHSNLKHPN ILRMYGHFHD KDNIYLILEY AGKGEFFKFL SDRGGKFGEK ETSLYIRQVM LALTYMKECN VIHRDIKPEN LLLGSDNQLK IADFGWAVYN ADKRRMTFCG TMEYLAPEMV NNDIHDSGID LWCLGILTYE FLMGKTPFES KNRNMREAYK KINSLKYTIP ETISSNASDF ISRLLVLSPG DRMELTEALN HPFIVKHHGR SSD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggagtac tggactttgg acaggtttga aataggaaga ttgcttgggc gtggaaagtt cgggcaggta tggcttgcaa gagagaggga aaaggggttt atagttgcgt tgaagattat tccaatcaag gaaatccaga cggtggagac ggcaaggcag atccgcagag agattgagat acactccaac ctgaagcatc ccaacattct gaggatgtat ggtcatttcc acgacaagga caatatatat ctgatactgg aatatgcagg gaagggcgag ttctttaagt tccttagcga ccggggaggc aagttcgggg agaaagagac atctctatac ataagacagg tgatgctggc gctgacatac atgaaggagt gtaatgtgat acacagagac ataaagccag agaatctgct gcttgggtca gacaaccagc tcaagatagc agattttgga tgggcggttt ataatgctga caagaggcgg atgaccttct gtggtacaat ggagtatctt gccccagaga tggttaacaa cgatatacac gactcaggga tagacctgtg gtgcctgggg atcctaacgt atgagtttct catgggaaaa actccgtttg aaagtaagaa caggaacatg agggaggcct ataagaaaat caactcactg aagtatacca ttccagagac gattagctcc aacgcttcag acttcatttc cagacttctt gtgttgagtc ctggcgacag aatggaactt acagaggctc taaatcatcc gtttattgta aagcaccacg ggaggtccag tgataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa