EncuA.01565.a

UDP-N-ACETYLGLUCOSAMINE PYROPHOSPHORYLASE

CENTER ID: EncuA.01565.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.01565.a.A1.GE27857 Full length( EncuA.01565.a ) 1 335
EncuA.01565.a.A1.GE28367 Full length( EncuA.01565.a ) 1 335
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.01565.a.A1.PS00444 Full length( EncuA.01565.a ) 1 335

External Resources

RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU10_0670
RefSeq: NP_586484.1
UniProt: Q8SQS1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGYISMNSTN LIRPYEGTEL NDAGRKYKKI GERLLREKKL GVVILSGGQG TRLGSDEPKG LFKIKGKTLF EWHMETIKEL ISKYNADIAV FIMTSSFTDE AVRKYFQSTD FGLKIQFFKQ RNSLCVGTDG KPLEWYDGHA ESPYGNGDIF NAIQQVNLEG IEALNVICID NVLAKILDPV FVGAFYSDDY DILSKSVTKE EKESVGAFLM DERLKIKEYS ENDAKGEGIQ GNICNHIFKT SFIKKMKNIN LPEHKAFKKI PYTISGKLIK PVKPNGFKKE TFIFDSFEYT QKNGVMNVPR EKEFSPLKNG MDSSVDNPVT CTIAVERHRI KTTIQ
NT Sequence
ATGGGATATA TTAGTATGAA TAGTACCAAT CTTATCAGGC CTTATGAAGG CACCGAGCTT AACGATGCGG GGAGGAAGTA CAAGAAGATC GGAGAAAGAC TTCTTAGAGA AAAGAAGCTG GGAGTCGTCA TACTGAGTGG GGGACAGGGG ACTAGGCTTG GGTCGGACGA GCCCAAGGGT CTGTTCAAAA TCAAGGGTAA AACATTATTT GAATGGCACA TGGAGACGAT AAAGGAGCTA ATAAGCAAGT ACAATGCAGA CATAGCCGTG TTCATCATGA CATCCTCGTT CACAGATGAG GCCGTCAGAA AGTACTTCCA GAGTACAGAC TTTGGTCTGA AGATCCAGTT CTTTAAGCAA AGAAACTCCT TGTGCGTGGG AACTGATGGA AAGCCCTTGG AGTGGTATGA CGGGCATGCC GAGTCGCCAT ATGGGAATGG AGACATCTTC AATGCCATAC AGCAGGTCAA TCTCGAAGGG ATTGAGGCCT TGAACGTCAT TTGCATAGAC AATGTTCTTG CAAAGATCCT TGATCCCGTG TTTGTCGGGG CATTCTACAG CGATGATTAT GATATTCTGA GTAAGTCGGT GACCAAGGAG GAGAAGGAAA GCGTCGGAGC CTTTCTGATG GATGAGAGGC TTAAGATCAA GGAGTATAGT GAGAACGATG CTAAGGGAGA GGGAATACAG GGCAACATAT GCAACCATAT CTTTAAAACT TCATTCATCA AGAAGATGAA GAATATCAAT CTTCCAGAAC ACAAGGCGTT CAAGAAGATC CCATACACCA TAAGCGGGAA GCTTATAAAG CCAGTCAAGC CTAACGGGTT CAAGAAGGAA ACATTTATAT TCGACAGCTT TGAATACACA CAGAAGAATG GCGTGATGAA TGTTCCTAGG GAAAAGGAGT TCTCGCCTTT GAAGAATGGA ATGGACTCAT CCGTGGACAA TCCGGTAACA TGCACGATAG CGGTTGAGAG GCATAGAATC AAAACAACAA TCCAA
Details for EncuA.01565.a.A1.GE27857
HARVESTED ON: 11/3/2009
SEQUENCED ON: 11/10/2009
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMGYISMN STNLIRPYEG TELNDAGRKY KKIGERLLRE KKLGVVILSG GQGTRLGSDE PKGLFKIKGK TLFEWHMETI KELISKYNAD IAVFIMTSSF TDEAVRKYFQ STDFGLKIQF FKQRNSLCVG TDGKPLEWYD GHAESPYGNG DIFNAIQQVN LEGIEALNVI CIDNVLAKIL DPVFVGAFYS DDYDILSKSV TKEEKESVGA FLMDERLKIK EYSENDAKGE GIQGNICNHI FKTSFIKKMK NINLPEHKAF KKIPYTISGK LIKPVKPNGF KKETFIFDSF EYTQKNGVMN VPREKEFSPL KNGMDSSVDN PVTCTIAVER HRIKTTIQ
Validated NT Sequence
tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgggata tattagtatg aatagtacca atcttatcag gccttatgaa ggcaccgagc ttaacgatgc ggggaggaag tacaagaaga tcggagaaag acttcttaga gaaaagaagc tgggagtcgt catactgagt gggggacagg ggactaggct tgggtcggac gagcccaagg gtctgttcaa aatcaagggt aaaacattat ttgaatggca catggagacg ataaaggagc taataagcaa gtacaatgca gacatagccg tgttcatcat gacatcctcg ttcacagatg aggccgtcag aaagtacttc cagagtacag actttggtct gaagatccag ttctttaagc aaagaaactc cttgtgcgtg ggaactgatg gaaagccctt ggagtggtat gacgggcatg ccgagtcgcc atatgggaat ggagacatct tcaatgccat acagcaggtc aatctcgaag ggattgaggc cttgaacgtc atttgcatag acaatgttct tgcaaagatc cttgatcccg tgtttgtcgg ggcattctac agcgatgatt atgatattct gagtaagtcg gtgaccaagg aggagaagga aagcgtcgga gcctttctga tggatgagag gcttaagatc aaggagtata gtgagaacga tgctaaggga gagggaatac agggcaacat atgcaaccat atctttaaaa cttcattcat caagaagatg aagaatatca atcttccaga acacaaggcg ttcaagaaga tcccatacac cataagcggg aagcttataa agccagtcaa gcctaacggg ttcaagaagg aaacatttat attcgacagc tttgaataca cacagaagaa tggcgtgatg aatgttccta gggaaaagga gttctcgcct ttgaagaatg gaatggactc atccgtggac aatccggtaa catgcacgat agcggttgag aggcatagaa tcaaaacaac aatccaata
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGYISMNST NLIRPYEGTE LNDAGRKYKK IGERLLREKK LGVVILSGGQ GTRLGSDEPK GLFKIKGKTL FEWHMETIKE LISKYNADIA VFIMTSSFTD EAVRKYFQST DFGLKIQFFK QRNSLCVGTD GKPLEWYDGH AESPYGNGDI FNAIQQVNLE GIEALNVICI DNVLAKILDP VFVGAFYSDD YDILSKSVTK EEKESVGAFL MDERLKIKEY SENDAKGEGI QGNICNHIFK TSFIKKMKNI NLPEHKAFKK IPYTISGKLI KPVKPNGFKK ETFIFDSFEY TQKNGVMNVP REKEFSPLKN GMDSSVDNPV TCTIAVERHR IKTTIQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggatatatt agtatgaata gtaccaatct tatcaggcct tatgaaggca ccgagcttaa cgatgcgggg aggaagtaca agaagatcgg agaaagactt cttagagaaa agaagctggg agtcgtcata ctgagtgggg gacaggggac taggcttggg tcggacgagc ccaagggtct gttcaaaatc aagggtaaaa cattatttga atggcacatg gagacgataa aggagctaat aagcaagtac aatgcagaca tagccgtgtt catcatgaca tcctcgttca cagatgaggc cgtcagaaag tacttccaga gtacagactt tggtctgaag atccagttct ttaagcaaag aaactccttg tgcgtgggaa ctgatggaaa gcccttggag tggtatgacg ggcatgccga gtcgccatat gggaatggag acatcttcaa tgccatacag caggtcaatc tcgaagggat tgaggccttg aacgtcattt gcatagacaa tgttcttgca aagatccttg atcccgtgtt tgtcggggca ttctacagcg atgattatga tattctgagt aagtcggtga ccaaggagga gaaggaaagc gtcggagcct ttctgatgga tgagaggctt aagatcaagg agtatagtga gaacgatgct aagggagagg gaatacaggg caacatatgc aaccatatct ttaaaacttc attcatcaag aagatgaaga atatcaatct tccagaacac aaggcgttca agaagatccc atacaccata agcgggaagc ttataaagcc agtcaagcct aacgggttca agaaggaaac atttatattc gacagctttg aatacacaca gaagaatggc gtgatgaatg ttcctaggga aaaggagttc tcgcctttga agaatggaat ggactcatcc gtggacaatc cggtaacatg cacgatagcg gttgagaggc atagaatcaa aacaacaatc caaaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for EncuA.01565.a.A1.GE28367
HARVESTED ON: 1/5/2010
SEQUENCED ON: 1/15/2010
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMGYISMN STNLIRPYEG TELNDAGRKY KKIGERLLRE KKLGVVILSG GQGTRLGSDE PKGLFKIKGK TLFEWHMETI KELISKYNAD IAVFIMTSSF TDEAVRKYFQ STDFGLKIQF FKQRNSLCVG TDGKPLEWYD GHAESPYGNG DIFNAIQQVN LEGIEALNVI CIDNVLAKIL DPVFVGAFYS DDYDILSKSV TKEEKESVGA FLMDERLKIK EYSENDAKGE GIQGNICNHI FKTSFIKKMK NINLPEHKAF KKIPYTISGK LIKPVKPNGF KKETFIFDSF EYTQKNGVMN VPREKEFSPL KNGMDSSVDN PVTCTIAVER HRIKTTIQ
Validated NT Sequence
tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgggata tattagtatg aatagtacca atcttatcag gccttatgaa ggcaccgagc ttaacgatgc ggggaggaag tacaagaaga tcggagaaag acttcttaga gaaaagaagc tgggagtcgt catactgagt gggggacagg ggactaggct tgggtcggac gagcccaagg gtctgttcaa aatcaagggt aaaacattat ttgaatggca catggagacg ataaaggagc taataagcaa gtacaatgca gacatagccg tgttcatcat gacatcctcg ttcacagatg aggccgtcag aaagtacttc cagagtacag actttggtct gaagatccag ttctttaagc aaagaaactc cttgtgcgtg ggaactgatg gaaagccctt ggagtggtat gacgggcatg ccgagtcgcc atatgggaat ggagacatct tcaatgccat acagcaggtc aatctcgaag ggattgaggc cttgaacgtc atttgcatag acaatgttct tgcaaagatc cttgatcccg tgtttgtcgg ggcattctac agcgatgatt atgatattct gagtaagtcg gtgaccaagg aggagaagga aagcgtcgga gcctttctga tggatgagag gcttaagatc aaggagtata gtgagaacga tgctaaggga gagggaatac agggcaacat atgcaaccat atctttaaaa cttcattcat caagaagatg aagaatatca atcttccaga acacaaggcg ttcaagaaga tcccatacac cataagcggg aagcttataa agccagtcaa gcctaacggg ttcaagaagg aaacatttat attcgacagc tttgaataca cacagaagaa tggcgtgatg aatgttccta gggaaaagga gttctcgcct ttgaagaatg gaatggactc atccgtggac aatccggtaa catgcacgat agcggttgag aggcatagaa tcaaaacaac aatccaata
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGYISMNST NLIRPYEGTE LNDAGRKYKK IGERLLREKK LGVVILSGGQ GTRLGSDEPK GLFKIKGKTL FEWHMETIKE LISKYNADIA VFIMTSSFTD EAVRKYFQST DFGLKIQFFK QRNSLCVGTD GKPLEWYDGH AESPYGNGDI FNAIQQVNLE GIEALNVICI DNVLAKILDP VFVGAFYSDD YDILSKSVTK EEKESVGAFL MDERLKIKEY SENDAKGEGI QGNICNHIFK TSFIKKMKNI NLPEHKAFKK IPYTISGKLI KPVKPNGFKK ETFIFDSFEY TQKNGVMNVP REKEFSPLKN GMDSSVDNPV TCTIAVERHR IKTTIQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggatatatt agtatgaata gtaccaatct tatcaggcct tatgaaggca ccgagcttaa cgatgcgggg aggaagtaca agaagatcgg agaaagactt cttagagaaa agaagctggg agtcgtcata ctgagtgggg gacaggggac taggcttggg tcggacgagc ccaagggtct gttcaaaatc aagggtaaaa cattatttga atggcacatg gagacgataa aggagctaat aagcaagtac aatgcagaca tagccgtgtt catcatgaca tcctcgttca cagatgaggc cgtcagaaag tacttccaga gtacagactt tggtctgaag atccagttct ttaagcaaag aaactccttg tgcgtgggaa ctgatggaaa gcccttggag tggtatgacg ggcatgccga gtcgccatat gggaatggag acatcttcaa tgccatacag caggtcaatc tcgaagggat tgaggccttg aacgtcattt gcatagacaa tgttcttgca aagatccttg atcccgtgtt tgtcggggca ttctacagcg atgattatga tattctgagt aagtcggtga ccaaggagga gaaggaaagc gtcggagcct ttctgatgga tgagaggctt aagatcaagg agtatagtga gaacgatgct aagggagagg gaatacaggg caacatatgc aaccatatct ttaaaacttc attcatcaag aagatgaaga atatcaatct tccagaacac aaggcgttca agaagatccc atacaccata agcgggaagc ttataaagcc agtcaagcct aacgggttca agaaggaaac atttatattc gacagctttg aatacacaca gaagaatggc gtgatgaatg ttcctaggga aaaggagttc tcgcctttga agaatggaat ggactcatcc gtggacaatc cggtaacatg cacgatagcg gttgagaggc atagaatcaa aacaacaatc caaaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for EncuA.01565.a.A1.PS00444
PURIFICATION DATe: 1/7/2010
CONCENTRATION: 21.25mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 12
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMGYISMN STNLIRPYEG TELNDAGRKY KKIGERLLRE KKLGVVILSG GQGTRLGSDE PKGLFKIKGK TLFEWHMETI KELISKYNAD IAVFIMTSSF TDEAVRKYFQ STDFGLKIQF FKQRNSLCVG TDGKPLEWYD GHAESPYGNG DIFNAIQQVN LEGIEALNVI CIDNVLAKIL DPVFVGAFYS DDYDILSKSV TKEEKESVGA FLMDERLKIK EYSENDAKGE GIQGNICNHI FKTSFIKKMK NINLPEHKAF KKIPYTISGK LIKPVKPNGF KKETFIFDSF EYTQKNGVMN VPREKEFSPL KNGMDSSVDN PVTCTIAVER HRIKTTIQ
Validated NT Sequence
tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgggata tattagtatg aatagtacca atcttatcag gccttatgaa ggcaccgagc ttaacgatgc ggggaggaag tacaagaaga tcggagaaag acttcttaga gaaaagaagc tgggagtcgt catactgagt gggggacagg ggactaggct tgggtcggac gagcccaagg gtctgttcaa aatcaagggt aaaacattat ttgaatggca catggagacg ataaaggagc taataagcaa gtacaatgca gacatagccg tgttcatcat gacatcctcg ttcacagatg aggccgtcag aaagtacttc cagagtacag actttggtct gaagatccag ttctttaagc aaagaaactc cttgtgcgtg ggaactgatg gaaagccctt ggagtggtat gacgggcatg ccgagtcgcc atatgggaat ggagacatct tcaatgccat acagcaggtc aatctcgaag ggattgaggc cttgaacgtc atttgcatag acaatgttct tgcaaagatc cttgatcccg tgtttgtcgg ggcattctac agcgatgatt atgatattct gagtaagtcg gtgaccaagg aggagaagga aagcgtcgga gcctttctga tggatgagag gcttaagatc aaggagtata gtgagaacga tgctaaggga gagggaatac agggcaacat atgcaaccat atctttaaaa cttcattcat caagaagatg aagaatatca atcttccaga acacaaggcg ttcaagaaga tcccatacac cataagcggg aagcttataa agccagtcaa gcctaacggg ttcaagaagg aaacatttat attcgacagc tttgaataca cacagaagaa tggcgtgatg aatgttccta gggaaaagga gttctcgcct ttgaagaatg gaatggactc atccgtggac aatccggtaa catgcacgat agcggttgag aggcatagaa tcaaaacaac aatccaata
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGYISMNST NLIRPYEGTE LNDAGRKYKK IGERLLREKK LGVVILSGGQ GTRLGSDEPK GLFKIKGKTL FEWHMETIKE LISKYNADIA VFIMTSSFTD EAVRKYFQST DFGLKIQFFK QRNSLCVGTD GKPLEWYDGH AESPYGNGDI FNAIQQVNLE GIEALNVICI DNVLAKILDP VFVGAFYSDD YDILSKSVTK EEKESVGAFL MDERLKIKEY SENDAKGEGI QGNICNHIFK TSFIKKMKNI NLPEHKAFKK IPYTISGKLI KPVKPNGFKK ETFIFDSFEY TQKNGVMNVP REKEFSPLKN GMDSSVDNPV TCTIAVERHR IKTTIQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGATATATT AGTATGAATA GTACCAATCT TATCAGGCCT TATGAAGGCA CCGAGCTTAA CGATGCGGGG AGGAAGTACA AGAAGATCGG AGAAAGACTT CTTAGAGAAA AGAAGCTGGG AGTCGTCATA CTGAGTGGGG GACAGGGGAC TAGGCTTGGG TCGGACGAGC CCAAGGGTCT GTTCAAAATC AAGGGTAAAA CATTATTTGA ATGGCACATG GAGACGATAA AGGAGCTAAT AAGCAAGTAC AATGCAGACA TAGCCGTGTT CATCATGACA TCCTCGTTCA CAGATGAGGC CGTCAGAAAG TACTTCCAGA GTACAGACTT TGGTCTGAAG ATCCAGTTCT TTAAGCAAAG AAACTCCTTG TGCGTGGGAA CTGATGGAAA GCCCTTGGAG TGGTATGACG GGCATGCCGA GTCGCCATAT GGGAATGGAG ACATCTTCAA TGCCATACAG CAGGTCAATC TCGAAGGGAT TGAGGCCTTG AACGTCATTT GCATAGACAA TGTTCTTGCA AAGATCCTTG ATCCCGTGTT TGTCGGGGCA TTCTACAGCG ATGATTATGA TATTCTGAGT AAGTCGGTGA CCAAGGAGGA GAAGGAAAGC GTCGGAGCCT TTCTGATGGA TGAGAGGCTT AAGATCAAGG AGTATAGTGA GAACGATGCT AAGGGAGAGG GAATACAGGG CAACATATGC AACCATATCT TTAAAACTTC ATTCATCAAG AAGATGAAGA ATATCAATCT TCCAGAACAC AAGGCGTTCA AGAAGATCCC ATACACCATA AGCGGGAAGC TTATAAAGCC AGTCAAGCCT AACGGGTTCA AGAAGGAAAC ATTTATATTC GACAGCTTTG AATACACACA GAAGAATGGC GTGATGAATG TTCCTAGGGA AAAGGAGTTC TCGCCTTTGA AGAATGGAAT GGACTCATCC GTGGACAATC CGGTAACATG CACGATAGCG GTTGAGAGGC ATAGAATCAA AACAACAATC CAAAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA