EncuA.01541.a

Deoxyhypusine synthase

CENTER ID: EncuA.01541.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.01541.a.A1.GE27855 Full length( EncuA.01541.a ) 1 362
EncuA.01541.a.A1.GE28366 Full length( EncuA.01541.a ) 1 362
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU09_0910
RefSeq: XP_955645.1
UniProt: Q8SQN2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MARAGQISVT QEAWVCTGDG KKSFPQLPMD RPQNVKDLAR KVKDNLSFSR EVRGMDFEKE WDFMAIMRSF ETMGFQGSNL YRAVEEIERM KNSKIFFGCT SNIISSGLRD VIATLVKRRH VHVLVITGGG IEEDIIKAFK PTFCADFRLD GAELRDNGLN RIGNLVIPSE NYEHLESWLN NIVNDITEGY TAERPRILTP SSFIRILGER IDDESSILYW AAKNDIPVYS PAVVDGSLGD ILSFHPRRKM LKLDIVEDVY RINCETIFCG ETAAIILGCG VVKHHILNAN LFKNGLEHCV LINNAQEFDG SDAGASLDEA VSWGKVKPGT RGVKVFGDAT ILFPLLVGAT FMRKDKDVPK GE
NT Sequence
ATGGCTCGCG CAGGTCAAAT ATCTGTAACC CAGGAAGCAT GGGTTTGCAC CGGAGACGGT AAAAAGAGCT TTCCACAGCT CCCCATGGAC AGGCCTCAGA ACGTTAAGGA CCTAGCGAGG AAGGTCAAGG ACAACCTCTC GTTCAGCAGA GAGGTAAGGG GAATGGACTT TGAGAAGGAG TGGGACTTCA TGGCGATAAT GAGATCGTTT GAGACGATGG GGTTCCAGGG ATCGAACTTG TACAGGGCAG TTGAGGAGAT TGAAAGGATG AAGAACTCGA AGATATTCTT TGGATGCACT AGCAACATAA TCAGCTCTGG ACTCAGGGAT GTGATTGCCA CACTGGTAAA GAGGAGGCAC GTCCATGTTC TTGTGATAAC AGGCGGAGGA ATCGAGGAGG ACATCATCAA GGCGTTCAAG CCGACATTCT GTGCAGACTT CAGGCTTGAT GGGGCAGAGC TTAGGGACAA CGGGCTGAAC AGAATAGGAA ACCTTGTGAT TCCAAGCGAG AACTACGAGC ACCTTGAGAG CTGGCTGAAC AACATTGTGA ACGACATAAC CGAGGGATAT ACGGCAGAGC GTCCGAGAAT ACTGACCCCA TCGTCATTTA TAAGGATTCT AGGGGAAAGA ATAGATGATG AATCATCAAT ACTTTATTGG GCAGCAAAGA ACGACATACC TGTGTACTCG CCTGCAGTTG TGGATGGCTC TCTTGGAGAC ATATTGTCGT TCCATCCCCG AAGGAAGATG CTGAAGCTGG ACATTGTCGA GGACGTGTAT CGGATTAACT GCGAGACCAT TTTCTGCGGG GAGACTGCGG CCATCATACT CGGGTGCGGG GTTGTGAAGC ACCACATCCT CAACGCTAAT CTATTCAAGA ACGGTCTTGA GCACTGTGTT CTGATCAACA ATGCCCAGGA GTTTGATGGA AGTGATGCAG GAGCCAGCCT GGACGAGGCA GTCAGCTGGG GTAAGGTCAA GCCCGGAACG AGAGGCGTAA AGGTGTTTGG CGATGCAACA ATACTGTTTC CACTGCTTGT TGGAGCAACC TTCATGAGGA AAGACAAGGA CGTTCCCAAA GGGGAG
Details for EncuA.01541.a.A1.GE27855
HARVESTED ON: 11/3/2009
SEQUENCED ON: 11/10/2009
EXPECTED MW: 42kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMARAGQI SVTQEAWVCT GDGKKSFPQL PMDRPQNVKD LARKVKDNLS FSREVRGMDF EKEWDFMAIM RSFETMGFQG SNLYRAVEEI ERMKNSKIFF GCTSNIISSG LRDVIATLVK RRHVHVLVIT GGGIEEDIIK AFKPTFCADF RLDGAELRDN GLNRIGNLVI PSENYEHLES WLNNIVNDIT EGYTAERPRI LTPSSFIRIL GERIDDESSI LYWAAKNDIP VYSPAVVDGS LGDILSFHPR RKMLKLDIVE DVYRINCETI FCGETAAIIL GCGVVKHHIL NANLFKNGLE HCVLINNAQE FDGSDAGASL DEAVSWGKVK PGTRGVKVFG DATILFPLLV GATFMRKDKD VPKGE
Validated NT Sequence
aaggagatat accntggctc atcaccatca ccatcatatg ggtaccctgg aagctcagac ccagggtcct ggttcgatgg ctcgcgcagg tcaaatatct gtaacccagg aagcatgggt ttgcaccgga gacggtaaaa agagctttcc acagctcccc atggacaggc ctcagaacgt taaggaccta gcgaggaagg tcaaggacaa cctctcgttc agcagagagg taaggggaat ggactttgag aaggagtggg acttcatggc gataatgaga tcgtttgaga cgatggggtt ccagggatcg aacttgtaca gggcagttga ggagattgaa aggatgaaga actcgaagat attctttgga tgcactagca acataatcag ctctggactc agggatgtga ttgccacact ggtaaagagg aggcacgtcc atgttcttgt gataacaggc ggaggaatcg aggaggacat catcaaggcg ttcaagccga cattctgtgc agacttcagg cttgatgggg cagagcttag ggacaacggg ctgaacagaa taggaaacct tgtgattcca agcgagaact acgagcacct tgagagctgg ctgaacaaca ttgtgaacga cataaccgag ggatatacgg cagagcgtcc gagaatactg accccatcgt catttataag gattctaggg gaaagaatag atgatgaatc atcaatactt tattgggcag caaagaacga catacctgtg tactcgcctg cagttgtgga tggctctctt ggagacatat tgtcgttcca tccccgaagg aagatgctga agctggacat tgtcgaggac gtgtatcgga ttaactgcga gaccattttc tgcggggaga ctgcggccat catactcggg tgcggggttg tgaagcacca catcctcaac gctaatctat tcaagaacgg tcttgagcac tgtgttctga tcaacaatgc ccaggagttt gatggaagtg atgcaggagc cagcctggac gaggcagtca gctggggtaa ggtcaagccc ggaacgagag gcgtaaaggt gtttggcgat gcaacaatac tgtttccact gcttgttgga gcaaccttca tgaggaaaga caaggacgtt cccaaagggg agtaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMARAGQISV TQEAWVCTGD GKKSFPQLPM DRPQNVKDLA RKVKDNLSFS REVRGMDFEK EWDFMAIMRS FETMGFQGSN LYRAVEEIER MKNSKIFFGC TSNIISSGLR DVIATLVKRR HVHVLVITGG GIEEDIIKAF KPTFCADFRL DGAELRDNGL NRIGNLVIPS ENYEHLESWL NNIVNDITEG YTAERPRILT PSSFIRILGE RIDDESSILY WAAKNDIPVY SPAVVDGSLG DILSFHPRRK MLKLDIVEDV YRINCETIFC GETAAIILGC GVVKHHILNA NLFKNGLEHC VLINNAQEFD GSDAGASLDE AVSWGKVKPG TRGVKVFGDA TILFPLLVGA TFMRKDKDVP KGE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctcgcgca ggtcaaatat ctgtaaccca ggaagcatgg gtttgcaccg gagacggtaa aaagagcttt ccacagctcc ccatggacag gcctcagaac gttaaggacc tagcgaggaa ggtcaaggac aacctctcgt tcagcagaga ggtaagggga atggactttg agaaggagtg ggacttcatg gcgataatga gatcgtttga gacgatgggg ttccagggat cgaacttgta cagggcagtt gaggagattg aaaggatgaa gaactcgaag atattctttg gatgcactag caacataatc agctctggac tcagggatgt gattgccaca ctggtaaaga ggaggcacgt ccatgttctt gtgataacag gcggaggaat cgaggaggac atcatcaagg cgttcaagcc gacattctgt gcagacttca ggcttgatgg ggcagagctt agggacaacg ggctgaacag aataggaaac cttgtgattc caagcgagaa ctacgagcac cttgagagct ggctgaacaa cattgtgaac gacataaccg agggatatac ggcagagcgt ccgagaatac tgaccccatc gtcatttata aggattctag gggaaagaat agatgatgaa tcatcaatac tttattgggc agcaaagaac gacatacctg tgtactcgcc tgcagttgtg gatggctctc ttggagacat attgtcgttc catccccgaa ggaagatgct gaagctggac attgtcgagg acgtgtatcg gattaactgc gagaccattt tctgcgggga gactgcggcc atcatactcg ggtgcggggt tgtgaagcac cacatcctca acgctaatct attcaagaac ggtcttgagc actgtgttct gatcaacaat gcccaggagt ttgatggaag tgatgcagga gccagcctgg acgaggcagt cagctggggt aaggtcaagc ccggaacgag aggcgtaaag gtgtttggcg atgcaacaat actgtttcca ctgcttgttg gagcaacctt catgaggaaa gacaaggacg ttcccaaagg ggagaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for EncuA.01541.a.A1.GE28366
HARVESTED ON: 1/5/2010
SEQUENCED ON: 1/15/2010
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMARAGQI SVTQEAWVCT GDGKKSFPQL PMDRPQNVKD LARKVKDNLS FSREVRGMDF EKEWDFMAIM RSFETMGFQG SNLYRAVEEI ERMKNSKIFF GCTSNIISSG LRDVIATLVK RRHVHVLVIT GGGIEEDIIK AFKPTFCADF RLDGAELRDN GLNRIGNLVI PSENYEHLES WLNNIVNDIT EGYTAERPRI LTPSSFIRIL GERIDDESSI LYWAAKNDIP VYSPAVVDGS LGDILSFHPR RKMLKLDIVE DVYRINCETI FCGETAAIIL GCGVVKHHIL NANLFKNGLE HCVLINNAQE FDGSDAGASL DEAVSWGKVK PGTRGVKVFG DATILFPLLV GATFMRKDKD VPKGE
Validated NT Sequence
aaggagatat accntggctc atcaccatca ccatcatatg ggtaccctgg aagctcagac ccagggtcct ggttcgatgg ctcgcgcagg tcaaatatct gtaacccagg aagcatgggt ttgcaccgga gacggtaaaa agagctttcc acagctcccc atggacaggc ctcagaacgt taaggaccta gcgaggaagg tcaaggacaa cctctcgttc agcagagagg taaggggaat ggactttgag aaggagtggg acttcatggc gataatgaga tcgtttgaga cgatggggtt ccagggatcg aacttgtaca gggcagttga ggagattgaa aggatgaaga actcgaagat attctttgga tgcactagca acataatcag ctctggactc agggatgtga ttgccacact ggtaaagagg aggcacgtcc atgttcttgt gataacaggc ggaggaatcg aggaggacat catcaaggcg ttcaagccga cattctgtgc agacttcagg cttgatgggg cagagcttag ggacaacggg ctgaacagaa taggaaacct tgtgattcca agcgagaact acgagcacct tgagagctgg ctgaacaaca ttgtgaacga cataaccgag ggatatacgg cagagcgtcc gagaatactg accccatcgt catttataag gattctaggg gaaagaatag atgatgaatc atcaatactt tattgggcag caaagaacga catacctgtg tactcgcctg cagttgtgga tggctctctt ggagacatat tgtcgttcca tccccgaagg aagatgctga agctggacat tgtcgaggac gtgtatcgga ttaactgcga gaccattttc tgcggggaga ctgcggccat catactcggg tgcggggttg tgaagcacca catcctcaac gctaatctat tcaagaacgg tcttgagcac tgtgttctga tcaacaatgc ccaggagttt gatggaagtg atgcaggagc cagcctggac gaggcagtca gctggggtaa ggtcaagccc ggaacgagag gcgtaaaggt gtttggcgat gcaacaatac tgtttccact gcttgttgga gcaaccttca tgaggaaaga caaggacgtt cccaaagggg agtaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMARAGQISV TQEAWVCTGD GKKSFPQLPM DRPQNVKDLA RKVKDNLSFS REVRGMDFEK EWDFMAIMRS FETMGFQGSN LYRAVEEIER MKNSKIFFGC TSNIISSGLR DVIATLVKRR HVHVLVITGG GIEEDIIKAF KPTFCADFRL DGAELRDNGL NRIGNLVIPS ENYEHLESWL NNIVNDITEG YTAERPRILT PSSFIRILGE RIDDESSILY WAAKNDIPVY SPAVVDGSLG DILSFHPRRK MLKLDIVEDV YRINCETIFC GETAAIILGC GVVKHHILNA NLFKNGLEHC VLINNAQEFD GSDAGASLDE AVSWGKVKPG TRGVKVFGDA TILFPLLVGA TFMRKDKDVP KGE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctcgcgca ggtcaaatat ctgtaaccca ggaagcatgg gtttgcaccg gagacggtaa aaagagcttt ccacagctcc ccatggacag gcctcagaac gttaaggacc tagcgaggaa ggtcaaggac aacctctcgt tcagcagaga ggtaagggga atggactttg agaaggagtg ggacttcatg gcgataatga gatcgtttga gacgatgggg ttccagggat cgaacttgta cagggcagtt gaggagattg aaaggatgaa gaactcgaag atattctttg gatgcactag caacataatc agctctggac tcagggatgt gattgccaca ctggtaaaga ggaggcacgt ccatgttctt gtgataacag gcggaggaat cgaggaggac atcatcaagg cgttcaagcc gacattctgt gcagacttca ggcttgatgg ggcagagctt agggacaacg ggctgaacag aataggaaac cttgtgattc caagcgagaa ctacgagcac cttgagagct ggctgaacaa cattgtgaac gacataaccg agggatatac ggcagagcgt ccgagaatac tgaccccatc gtcatttata aggattctag gggaaagaat agatgatgaa tcatcaatac tttattgggc agcaaagaac gacatacctg tgtactcgcc tgcagttgtg gatggctctc ttggagacat attgtcgttc catccccgaa ggaagatgct gaagctggac attgtcgagg acgtgtatcg gattaactgc gagaccattt tctgcgggga gactgcggcc atcatactcg ggtgcggggt tgtgaagcac cacatcctca acgctaatct attcaagaac ggtcttgagc actgtgttct gatcaacaat gcccaggagt ttgatggaag tgatgcagga gccagcctgg acgaggcagt cagctggggt aaggtcaagc ccggaacgag aggcgtaaag gtgtttggcg atgcaacaat actgtttcca ctgcttgttg gagcaacctt catgaggaaa gacaaggacg ttcccaaagg ggagaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa