EncuA.01483.a

Diphthine synthase

CENTER ID: EncuA.01483.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.01483.a.A1.GE27850 Full length( EncuA.01483.a ) 1 262
EncuA.01483.a.A1.GE28363 Full length( EncuA.01483.a ) 1 262
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.01483.a.A1.PS00459 Full length( EncuA.01483.a ) 1 262
External Resources
RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU11_1430
OrthoMCL: OG5_127443
RefSeq: NP_586449.1
UniProt: Q8SQT9
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGDKSPIPKP PMLYLVGFGL HSYKDITLRG LEAVKGSATV YLESYTSIHG ESLDAFEKLI GKKVHLADRA MMEQTDKIVD ESCRENVSLL VVGTPLFATT HSDIMIRAKE KGVDVEVIHN ASIINVLGCC GLYSYSFGRV VSIPYFTERW KPTSFYDNIV RNHQSNLHTL CLLDIRTDED RFMSVNEAVD QILEAAAITG SPLINEDTRI FAVCRFGSPS EEIVYGKIGD LKLRSFGDPL HSLIVPAELD RVEAELVGSL FK
NT Sequence
ATGGGTGATA AATCTCCCAT CCCAAAACCT CCCATGCTCT ACCTGGTTGG ATTTGGATTG CACTCGTACA AGGACATAAC TCTCAGGGGA CTGGAGGCTG TCAAAGGCAG TGCCACGGTG TATCTCGAAA GCTACACATC CATCCACGGC GAGTCGCTGG ATGCGTTTGA AAAGCTCATA GGGAAGAAGG TGCATCTTGC AGACCGAGCC ATGATGGAAC AGACAGACAA AATAGTTGAC GAGTCCTGCA GGGAGAACGT CTCCCTACTT GTTGTTGGAA CTCCGCTGTT TGCAACAACC CACTCGGACA TAATGATAAG GGCAAAGGAG AAGGGCGTGG ATGTGGAGGT GATTCACAAC GCCTCCATCA TAAACGTGTT GGGCTGCTGC GGGCTCTACT CATACTCCTT TGGAAGAGTA GTGTCCATCC CATACTTCAC AGAGAGGTGG AAGCCAACAA GCTTTTACGA CAATATAGTG AGGAACCACC AGAGCAACCT TCATACACTA TGTCTCCTGG ATATCAGGAC AGATGAGGAC AGGTTCATGA GCGTTAACGA GGCCGTCGAC CAGATTCTGG AGGCAGCCGC CATTACAGGG TCTCCGCTTA TCAACGAAGA CACCAGGATC TTTGCAGTCT GCAGATTCGG ATCGCCTTCA GAAGAAATAG TCTACGGGAA AATCGGGGAC CTGAAGCTGA GGTCGTTTGG GGACCCCCTG CACTCATTGA TAGTACCTGC AGAGCTGGAC AGGGTTGAGG CGGAGCTGGT TGGATCCCTA TTTAAG
Details for EncuA.01483.a.A1.GE27850
HARVESTED ON: 11/3/2009
SEQUENCED ON: 11/10/2009
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGDKSPIPK PPMLYLVGFG LHSYKDITLR GLEAVKGSAT VYLESYTSIH GESLDAFEKL IGKKVHLADR AMMEQTDKIV DESCRENVSL LVVGTPLFAT THSDIMIRAK EKGVDVEVIH NASIINVLGC CGLYSYSFGR VVSIPYFTER WKPTSFYDNI VRNHQSNLHT LCLLDIRTDE DRFMSVNEAV DQILEAAAIT GSPLINEDTR IFAVCRFGSP SEEIVYGKIG DLKLRSFGDP LHSLIVPAEL DRVEAELVGS LFK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgggtg ataaatctcc catcccaaaa cctcccatgc tctacctggt tggatttgga ttgcactcgt acaaggacat aactctcagg ggactggagg ctgtcaaagg cagtgccacg gtgtatctcg aaagctacac atccatccac ggcgagtcgc tggatgcgtt tgaaaagctc atagggaaga aggtgcatct tgcagaccga gccatgatgg aacagacaga caaaatagtt gacgagtcct gcagggagaa cgtctcccta cttgttgttg gaactccgct gtttgcaaca acccactcgg acataatgat aagggcaaag gagaagggcg tggatgtgga ggtgattcac aacgcctcca tcataaacgt gttgggctgc tgcgggctct actcatactc ctttggaaga gtagtgtcca tcccatactt cacagagagg tggaagccaa caagctttta cgacaatata gtgaggaacc accagagcaa ccttcataca ctatgtctcc tggatatcag gacagatgag gacaggttca tgagcgttaa cgaggccgtc gaccagattc tggaggcagc cgccattaca gggtctccgc ttatcaacga agacaccagg atctttgcag tctgcagatt cggatcgcct tcagaagaaa tagtctacgg gaaaatcggg gacctgaagc tgaggtcgtt tggggacccc ctgcactcat tgatagtacc tgcagagctg gacagggttg aggcggagct ggttggatcc ctatttaagt aataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGDKSPIPK PPMLYLVGFG LHSYKDITLR GLEAVKGSAT VYLESYTSIH GESLDAFEKL IGKKVHLADR AMMEQTDKIV DESCRENVSL LVVGTPLFAT THSDIMIRAK EKGVDVEVIH NASIINVLGC CGLYSYSFGR VVSIPYFTER WKPTSFYDNI VRNHQSNLHT LCLLDIRTDE DRFMSVNEAV DQILEAAAIT GSPLINEDTR IFAVCRFGSP SEEIVYGKIG DLKLRSFGDP LHSLIVPAEL DRVEAELVGS LFK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggtgataaa tctcccatcc caaaacctcc catgctctac ctggttggat ttggattgca ctcgtacaag gacataactc tcaggggact ggaggctgtc aaaggcagtg ccacggtgta tctcgaaagc tacacatcca tccacggcga gtcgctggat gcgtttgaaa agctcatagg gaagaaggtg catcttgcag accgagccat gatggaacag acagacaaaa tagttgacga gtcctgcagg gagaacgtct ccctacttgt tgttggaact ccgctgtttg caacaaccca ctcggacata atgataaggg caaaggagaa gggcgtggat gtggaggtga ttcacaacgc ctccatcata aacgtgttgg gctgctgcgg gctctactca tactcctttg gaagagtagt gtccatccca tacttcacag agaggtggaa gccaacaagc ttttacgaca atatagtgag gaaccaccag agcaaccttc atacactatg tctcctggat atcaggacag atgaggacag gttcatgagc gttaacgagg ccgtcgacca gattctggag gcagccgcca ttacagggtc tccgcttatc aacgaagaca ccaggatctt tgcagtctgc agattcggat cgccttcaga agaaatagtc tacgggaaaa tcggggacct gaagctgagg tcgtttgggg accccctgca ctcattgata gtacctgcag agctggacag ggttgaggcg gagctggttg gatccctatt taagaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for EncuA.01483.a.A1.GE28363
HARVESTED ON: 1/5/2010
SEQUENCED ON: 1/15/2010
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGDKSPIPK PPMLYLVGFG LHSYKDITLR GLEAVKGSAT VYLESYTSIH GESLDAFEKL IGKKVHLADR AMMEQTDKIV DESCRENVSL LVVGTPLFAT THSDIMIRAK EKGVDVEVIH NASIINVLGC CGLYSYSFGR VVSIPYFTER WKPTSFYDNI VRNHQSNLHT LCLLDIRTDE DRFMSVNEAV DQILEAAAIT GSPLINEDTR IFAVCRFGSP SEEIVYGKIG DLKLRSFGDP LHSLIVPAEL DRVEAELVGS LFK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgggtg ataaatctcc catcccaaaa cctcccatgc tctacctggt tggatttgga ttgcactcgt acaaggacat aactctcagg ggactggagg ctgtcaaagg cagtgccacg gtgtatctcg aaagctacac atccatccac ggcgagtcgc tggatgcgtt tgaaaagctc atagggaaga aggtgcatct tgcagaccga gccatgatgg aacagacaga caaaatagtt gacgagtcct gcagggagaa cgtctcccta cttgttgttg gaactccgct gtttgcaaca acccactcgg acataatgat aagggcaaag gagaagggcg tggatgtgga ggtgattcac aacgcctcca tcataaacgt gttgggctgc tgcgggctct actcatactc ctttggaaga gtagtgtcca tcccatactt cacagagagg tggaagccaa caagctttta cgacaatata gtgaggaacc accagagcaa ccttcataca ctatgtctcc tggatatcag gacagatgag gacaggttca tgagcgttaa cgaggccgtc gaccagattc tggaggcagc cgccattaca gggtctccgc ttatcaacga agacaccagg atctttgcag tctgcagatt cggatcgcct tcagaagaaa tagtctacgg gaaaatcggg gacctgaagc tgaggtcgtt tggggacccc ctgcactcat tgatagtacc tgcagagctg gacagggttg aggcggagct ggttggatcc ctatttaagt aataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGDKSPIPK PPMLYLVGFG LHSYKDITLR GLEAVKGSAT VYLESYTSIH GESLDAFEKL IGKKVHLADR AMMEQTDKIV DESCRENVSL LVVGTPLFAT THSDIMIRAK EKGVDVEVIH NASIINVLGC CGLYSYSFGR VVSIPYFTER WKPTSFYDNI VRNHQSNLHT LCLLDIRTDE DRFMSVNEAV DQILEAAAIT GSPLINEDTR IFAVCRFGSP SEEIVYGKIG DLKLRSFGDP LHSLIVPAEL DRVEAELVGS LFK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggtgataaa tctcccatcc caaaacctcc catgctctac ctggttggat ttggattgca ctcgtacaag gacataactc tcaggggact ggaggctgtc aaaggcagtg ccacggtgta tctcgaaagc tacacatcca tccacggcga gtcgctggat gcgtttgaaa agctcatagg gaagaaggtg catcttgcag accgagccat gatggaacag acagacaaaa tagttgacga gtcctgcagg gagaacgtct ccctacttgt tgttggaact ccgctgtttg caacaaccca ctcggacata atgataaggg caaaggagaa gggcgtggat gtggaggtga ttcacaacgc ctccatcata aacgtgttgg gctgctgcgg gctctactca tactcctttg gaagagtagt gtccatccca tacttcacag agaggtggaa gccaacaagc ttttacgaca atatagtgag gaaccaccag agcaaccttc atacactatg tctcctggat atcaggacag atgaggacag gttcatgagc gttaacgagg ccgtcgacca gattctggag gcagccgcca ttacagggtc tccgcttatc aacgaagaca ccaggatctt tgcagtctgc agattcggat cgccttcaga agaaatagtc tacgggaaaa tcggggacct gaagctgagg tcgtttgggg accccctgca ctcattgata gtacctgcag agctggacag ggttgaggcg gagctggttg gatccctatt taagaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for EncuA.01483.a.A1.PS00459
PURIFICATION DATe: 1/22/2010
CONCENTRATION: 38.45mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGDKSPIPK PPMLYLVGFG LHSYKDITLR GLEAVKGSAT VYLESYTSIH GESLDAFEKL IGKKVHLADR AMMEQTDKIV DESCRENVSL LVVGTPLFAT THSDIMIRAK EKGVDVEVIH NASIINVLGC CGLYSYSFGR VVSIPYFTER WKPTSFYDNI VRNHQSNLHT LCLLDIRTDE DRFMSVNEAV DQILEAAAIT GSPLINEDTR IFAVCRFGSP SEEIVYGKIG DLKLRSFGDP LHSLIVPAEL DRVEAELVGS LFK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgggtg ataaatctcc catcccaaaa cctcccatgc tctacctggt tggatttgga ttgcactcgt acaaggacat aactctcagg ggactggagg ctgtcaaagg cagtgccacg gtgtatctcg aaagctacac atccatccac ggcgagtcgc tggatgcgtt tgaaaagctc atagggaaga aggtgcatct tgcagaccga gccatgatgg aacagacaga caaaatagtt gacgagtcct gcagggagaa cgtctcccta cttgttgttg gaactccgct gtttgcaaca acccactcgg acataatgat aagggcaaag gagaagggcg tggatgtgga ggtgattcac aacgcctcca tcataaacgt gttgggctgc tgcgggctct actcatactc ctttggaaga gtagtgtcca tcccatactt cacagagagg tggaagccaa caagctttta cgacaatata gtgaggaacc accagagcaa ccttcataca ctatgtctcc tggatatcag gacagatgag gacaggttca tgagcgttaa cgaggccgtc gaccagattc tggaggcagc cgccattaca gggtctccgc ttatcaacga agacaccagg atctttgcag tctgcagatt cggatcgcct tcagaagaaa tagtctacgg gaaaatcggg gacctgaagc tgaggtcgtt tggggacccc ctgcactcat tgatagtacc tgcagagctg gacagggttg aggcggagct ggttggatcc ctatttaagt aataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGDKSPIPK PPMLYLVGFG LHSYKDITLR GLEAVKGSAT VYLESYTSIH GESLDAFEKL IGKKVHLADR AMMEQTDKIV DESCRENVSL LVVGTPLFAT THSDIMIRAK EKGVDVEVIH NASIINVLGC CGLYSYSFGR VVSIPYFTER WKPTSFYDNI VRNHQSNLHT LCLLDIRTDE DRFMSVNEAV DQILEAAAIT GSPLINEDTR IFAVCRFGSP SEEIVYGKIG DLKLRSFGDP LHSLIVPAEL DRVEAELVGS LFK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGTGATAAA TCTCCCATCC CAAAACCTCC CATGCTCTAC CTGGTTGGAT TTGGATTGCA CTCGTACAAG GACATAACTC TCAGGGGACT GGAGGCTGTC AAAGGCAGTG CCACGGTGTA TCTCGAAAGC TACACATCCA TCCACGGCGA GTCGCTGGAT GCGTTTGAAA AGCTCATAGG GAAGAAGGTG CATCTTGCAG ACCGAGCCAT GATGGAACAG ACAGACAAAA TAGTTGACGA GTCCTGCAGG GAGAACGTCT CCCTACTTGT TGTTGGAACT CCGCTGTTTG CAACAACCCA CTCGGACATA ATGATAAGGG CAAAGGAGAA GGGCGTGGAT GTGGAGGTGA TTCACAACGC CTCCATCATA AACGTGTTGG GCTGCTGCGG GCTCTACTCA TACTCCTTTG GAAGAGTAGT GTCCATCCCA TACTTCACAG AGAGGTGGAA GCCAACAAGC TTTTACGACA ATATAGTGAG GAACCACCAG AGCAACCTTC ATACACTATG TCTCCTGGAT ATCAGGACAG ATGAGGACAG GTTCATGAGC GTTAACGAGG CCGTCGACCA GATTCTGGAG GCAGCCGCCA TTACAGGGTC TCCGCTTATC AACGAAGACA CCAGGATCTT TGCAGTCTGC AGATTCGGAT CGCCTTCAGA AGAAATAGTC TACGGGAAAA TCGGGGACCT GAAGCTGAGG TCGTTTGGGG ACCCCCTGCA CTCATTGATA GTACCTGCAG AGCTGGACAG GGTTGAGGCG GAGCTGGTTG GATCCCTATT TAAGAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA