EncuA.01479.a

SER/THR PROTEIN KINASE

CENTER ID: EncuA.01479.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.01479.a.A1.GE27849 Full length( EncuA.01479.a ) 1 405
EncuA.01479.a.A1.GE28362 Full length( EncuA.01479.a ) 1 405
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU11_0180
RefSeq: NP_586324.1
UniProt: Q8SQZ4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKKVLTFPS EKIGFSSDNE EFDLVMARGD YLAKNKSQKY MIIDMLGTGT FGQVVRCVGS DGEEVAIKVV KNQPKYYNYE MNEVRILHKL LYNNLNDRFV TIKDVFMYKQ HLCIVEELLG RNLYTFLKMT RFKGLDHPTL RTILHQILEG MVQLSLLGII HCDLKPENIL IADYDTFKIK IIDFGSAVTS PQGSHFYVQS RYYRAPEVIL GIPYGSSCDI WSLGCIGYEL YVGHPLFPGK DNMDQIGRIH GLFGSLPMFM LEHGKNSSTF FEKENGYRFM GPPSNFTLED MKKMIRSKGN SKEDDNMLIK FLLRALQPSH LIRPDAKSLA SHSYLKVRDT SAEADKACND RSGVQQNIFP ANKNMRHMST TGVILPSKKQ KPTDDRRKIS VYGISYENNL NRDSE
NT Sequence
ATGAAGAAGA AAGTTTTGAC ATTTCCATCT GAAAAGATAG GTTTCTCGTC TGATAATGAA GAATTTGATC TTGTGATGGC CCGAGGAGAC TATCTAGCAA AGAATAAAAG CCAGAAATAC ATGATTATCG ACATGCTGGG AACAGGAACC TTTGGACAAG TTGTCAGGTG CGTTGGCTCT GATGGAGAGG AAGTCGCAAT TAAGGTAGTA AAAAATCAGC CGAAATACTA CAACTACGAG ATGAACGAGG TCCGGATTCT CCATAAACTG CTGTACAACA ACTTAAATGA TAGATTTGTA ACCATTAAGG ATGTTTTCAT GTACAAGCAG CACCTCTGTA TTGTCGAAGA GCTACTAGGC AGGAATCTGT ATACATTTTT GAAAATGACA AGGTTCAAGG GCTTGGACCA TCCTACCCTC AGAACCATAC TTCATCAGAT ACTTGAAGGA ATGGTTCAGC TTTCACTGTT GGGAATCATT CACTGTGACC TGAAGCCTGA AAACATCCTG ATAGCGGATT ATGACACCTT CAAGATAAAG ATCATCGATT TTGGATCGGC CGTCACTTCT CCCCAGGGGT CCCACTTTTA CGTTCAGTCA AGATACTATC GCGCCCCAGA GGTTATCCTC GGGATTCCAT ATGGATCGAG TTGTGATATA TGGTCTCTTG GATGTATAGG ATATGAATTA TATGTTGGGC ATCCGTTGTT CCCAGGTAAA GACAACATGG ATCAAATAGG AAGAATTCAT GGGCTATTTG GATCCTTACC TATGTTCATG CTCGAGCACG GAAAGAACTC GAGCACCTTC TTTGAAAAAG AAAACGGATA CAGATTCATG GGACCACCAT CGAACTTTAC TTTAGAGGAT ATGAAAAAAA TGATCAGGTC GAAAGGCAAT TCAAAGGAAG ATGATAACAT GCTCATAAAG TTTCTACTGA GAGCACTGCA GCCCAGTCAT CTCATAAGGC CAGATGCCAA ATCACTAGCA AGCCACTCTT ATCTTAAGGT CAGGGACACA TCCGCTGAAG CCGATAAGGC CTGTAACGAT CGTAGTGGAG TCCAGCAGAA TATTTTCCCT GCAAACAAAA ACATGAGGCA CATGTCTACA ACAGGAGTAA TTCTTCCTAG TAAGAAACAA AAGCCCACCG ATGACAGGAG GAAAATCTCG GTTTATGGAA TTTCGTATGA GAACAATCTA AATAGAGACT CAGAA
Details for EncuA.01479.a.A1.GE27849
HARVESTED ON: 11/3/2009
SEQUENCED ON: 11/10/2009
EXPECTED MW: 48kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MGTLEAQTQG PGSMKKKVLT FPSEKIGFSS DNEEFDLVMA RGDYLAKNKS QKYMIIDMLG TGTFGQVVRC VGSDGEEVAI KVVKNQPKYY NYEMNEVRIL HKLLYNNLND RFVTIKDVFM YKQHLCIVEE LLGRNLYTFL KMTRFKGLDH PTLRTILHQI LEGMVQLSLL GIIHCDLKPE NILIADYDTF KIKIIDFGSA VTSPQGSHFY VQSRYYRAPE VILGIPYGSS CDIWSLGCIG YELYVGHPLF PGKDNMDQIG RIHGLFGSLP MFMLEHGKNS STFFEKENGY RFMGPPSNFT LEDMKKMIRS KGNSKEDDNM LIKFLLRALQ PSHLIRPDAK SLASHSYLKV RDTSAEADKA CNDRSGVQQN IFPANKNMRH MSTTGVILPS KKQKPTDDRR KISVYGISYE NNLNRDSE
Validated NT Sequence
ttattattct gagtctctat ttagattgtt ctcatacgaa attccataaa ccgagatttt cctcctgtca tcggtgggct tttgtttctt actaggaaga attactcctg ttgtagacat gtgcctcatg tttttgtttg cagggaaaat attctgctgg actccactac gatcgttaca ggccttatcg gcttcagcgg atgtgtccct gaccttaaga taagagtggc ttgctagtga tttggcatct ggccttatga gatgactggg ctgcagtgct ctcagtagaa actttatgag catgttatca tcttcctttg aattgccttt cgacctgatc atttttttca tatcctctaa agtaaagttc gatggtggtc ccatgaatct gtatccgttt tctttttcaa agaaggtgct cgagttcttt ccgtgctcga gcatgaacat aggtaaggat ccaaatagcc catgaattct tcctatttga tccatgttgt ctttacctgg gaacaacgga tgcccaacat ataattcata tcctatacat ccaagagacc atatatcaca actcgatcca tatggaatcc cgaggataac ctctggggcg cgatagtatc ttgactgaac gtaaaagtgg gacccctggg gagaagtgac ggccgatcca aaatcgatga tctttatctt gaaggtgtca taatccgcta tcaggatgtt ttcaggcttc aggtcacagt gaatgattcc caacagtgaa agctgaacca ttccttcaag tatctgatga agtatggttc tgagggtagg atggtccaag cccttgaacc ttgtcatttt caaaaatgta tacagattcc tgcctagtag ctcttcgaca atacagaggt gctgcttgta catgaaaaca tccttaatgg ttacaaatct atcatttaag ttgttgtaca gcagtttatg gagaatccgg acctcgttca tctcgtagtt gtagtatttc ggctgatttt ttactacctt aattgcgact tcctctccat cagagccaac gcacctgaca acttgtccaa aggttcctgt tcccagcatg tcgataatca tgtatttctg gcttttattc tttgctagat agtctcctcg ggccatcaca agatcaaatt cttcattatc agacgagaaa cctatctttt cagatggaaa tgtcaaaact ttcttcttca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag cca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKKKVLTFP SEKIGFSSDN EEFDLVMARG DYLAKNKSQK YMIIDMLGTG TFGQVVRCVG SDGEEVAIKV VKNQPKYYNY EMNEVRILHK LLYNNLNDRF VTIKDVFMYK QHLCIVEELL GRNLYTFLKM TRFKGLDHPT LRTILHQILE GMVQLSLLGI IHCDLKPENI LIADYDTFKI KIIDFGSAVT SPQGSHFYVQ SRYYRAPEVI LGIPYGSSCD IWSLGCIGYE LYVGHPLFPG KDNMDQIGRI HGLFGSLPMF MLEHGKNSST FFEKENGYRF MGPPSNFTLE DMKKMIRSKG NSKEDDNMLI KFLLRALQPS HLIRPDAKSL ASHSYLKVRD TSAEADKACN DRSGVQQNIF PANKNMRHMS TTGVILPSKK QKPTDDRRKI SVYGISYENN LNRDSE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagaagaaa gttttgacat ttccatctga aaagataggt ttctcgtctg ataatgaaga atttgatctt gtgatggccc gaggagacta tctagcaaag aataaaagcc agaaatacat gattatcgac atgctgggaa caggaacctt tggacaagtt gtcaggtgcg ttggctctga tggagaggaa gtcgcaatta aggtagtaaa aaatcagccg aaatactaca actacgagat gaacgaggtc cggattctcc ataaactgct gtacaacaac ttaaatgata gatttgtaac cattaaggat gttttcatgt acaagcagca cctctgtatt gtcgaagagc tactaggcag gaatctgtat acatttttga aaatgacaag gttcaagggc ttggaccatc ctaccctcag aaccatactt catcagatac ttgaaggaat ggttcagctt tcactgttgg gaatcattca ctgtgacctg aagcctgaaa acatcctgat agcggattat gacaccttca agataaagat catcgatttt ggatcggccg tcacttctcc ccaggggtcc cacttttacg ttcagtcaag atactatcgc gccccagagg ttatcctcgg gattccatat ggatcgagtt gtgatatatg gtctcttgga tgtataggat atgaattata tgttgggcat ccgttgttcc caggtaaaga caacatggat caaataggaa gaattcatgg gctatttgga tccttaccta tgttcatgct cgagcacgga aagaactcga gcaccttctt tgaaaaagaa aacggataca gattcatggg accaccatcg aactttactt tagaggatat gaaaaaaatg atcaggtcga aaggcaattc aaaggaagat gataacatgc tcataaagtt tctactgaga gcactgcagc ccagtcatct cataaggcca gatgccaaat cactagcaag ccactcttat cttaaggtca gggacacatc cgctgaagcc gataaggcct gtaacgatcg tagtggagtc cagcagaata ttttccctgc aaacaaaaac atgaggcaca tgtctacaac aggagtaatt cttcctagta agaaacaaaa gcccaccgat gacaggagga aaatctcggt ttatggaatt tcgtatgaga acaatctaaa tagagactca gaaaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for EncuA.01479.a.A1.GE28362
HARVESTED ON: 1/5/2010
SEQUENCED ON: 1/15/2010
EXPECTED MW: 48kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MGTLEAQTQG PGSMKKKVLT FPSEKIGFSS DNEEFDLVMA RGDYLAKNKS QKYMIIDMLG TGTFGQVVRC VGSDGEEVAI KVVKNQPKYY NYEMNEVRIL HKLLYNNLND RFVTIKDVFM YKQHLCIVEE LLGRNLYTFL KMTRFKGLDH PTLRTILHQI LEGMVQLSLL GIIHCDLKPE NILIADYDTF KIKIIDFGSA VTSPQGSHFY VQSRYYRAPE VILGIPYGSS CDIWSLGCIG YELYVGHPLF PGKDNMDQIG RIHGLFGSLP MFMLEHGKNS STFFEKENGY RFMGPPSNFT LEDMKKMIRS KGNSKEDDNM LIKFLLRALQ PSHLIRPDAK SLASHSYLKV RDTSAEADKA CNDRSGVQQN IFPANKNMRH MSTTGVILPS KKQKPTDDRR KISVYGISYE NNLNRDSE
Validated NT Sequence
ttattattct gagtctctat ttagattgtt ctcatacgaa attccataaa ccgagatttt cctcctgtca tcggtgggct tttgtttctt actaggaaga attactcctg ttgtagacat gtgcctcatg tttttgtttg cagggaaaat attctgctgg actccactac gatcgttaca ggccttatcg gcttcagcgg atgtgtccct gaccttaaga taagagtggc ttgctagtga tttggcatct ggccttatga gatgactggg ctgcagtgct ctcagtagaa actttatgag catgttatca tcttcctttg aattgccttt cgacctgatc atttttttca tatcctctaa agtaaagttc gatggtggtc ccatgaatct gtatccgttt tctttttcaa agaaggtgct cgagttcttt ccgtgctcga gcatgaacat aggtaaggat ccaaatagcc catgaattct tcctatttga tccatgttgt ctttacctgg gaacaacgga tgcccaacat ataattcata tcctatacat ccaagagacc atatatcaca actcgatcca tatggaatcc cgaggataac ctctggggcg cgatagtatc ttgactgaac gtaaaagtgg gacccctggg gagaagtgac ggccgatcca aaatcgatga tctttatctt gaaggtgtca taatccgcta tcaggatgtt ttcaggcttc aggtcacagt gaatgattcc caacagtgaa agctgaacca ttccttcaag tatctgatga agtatggttc tgagggtagg atggtccaag cccttgaacc ttgtcatttt caaaaatgta tacagattcc tgcctagtag ctcttcgaca atacagaggt gctgcttgta catgaaaaca tccttaatgg ttacaaatct atcatttaag ttgttgtaca gcagtttatg gagaatccgg acctcgttca tctcgtagtt gtagtatttc ggctgatttt ttactacctt aattgcgact tcctctccat cagagccaac gcacctgaca acttgtccaa aggttcctgt tcccagcatg tcgataatca tgtatttctg gcttttattc tttgctagat agtctcctcg ggccatcaca agatcaaatt cttcattatc agacgagaaa cctatctttt cagatggaaa tgtcaaaact ttcttcttca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag cca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKKKVLTFP SEKIGFSSDN EEFDLVMARG DYLAKNKSQK YMIIDMLGTG TFGQVVRCVG SDGEEVAIKV VKNQPKYYNY EMNEVRILHK LLYNNLNDRF VTIKDVFMYK QHLCIVEELL GRNLYTFLKM TRFKGLDHPT LRTILHQILE GMVQLSLLGI IHCDLKPENI LIADYDTFKI KIIDFGSAVT SPQGSHFYVQ SRYYRAPEVI LGIPYGSSCD IWSLGCIGYE LYVGHPLFPG KDNMDQIGRI HGLFGSLPMF MLEHGKNSST FFEKENGYRF MGPPSNFTLE DMKKMIRSKG NSKEDDNMLI KFLLRALQPS HLIRPDAKSL ASHSYLKVRD TSAEADKACN DRSGVQQNIF PANKNMRHMS TTGVILPSKK QKPTDDRRKI SVYGISYENN LNRDSE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagaagaaa gttttgacat ttccatctga aaagataggt ttctcgtctg ataatgaaga atttgatctt gtgatggccc gaggagacta tctagcaaag aataaaagcc agaaatacat gattatcgac atgctgggaa caggaacctt tggacaagtt gtcaggtgcg ttggctctga tggagaggaa gtcgcaatta aggtagtaaa aaatcagccg aaatactaca actacgagat gaacgaggtc cggattctcc ataaactgct gtacaacaac ttaaatgata gatttgtaac cattaaggat gttttcatgt acaagcagca cctctgtatt gtcgaagagc tactaggcag gaatctgtat acatttttga aaatgacaag gttcaagggc ttggaccatc ctaccctcag aaccatactt catcagatac ttgaaggaat ggttcagctt tcactgttgg gaatcattca ctgtgacctg aagcctgaaa acatcctgat agcggattat gacaccttca agataaagat catcgatttt ggatcggccg tcacttctcc ccaggggtcc cacttttacg ttcagtcaag atactatcgc gccccagagg ttatcctcgg gattccatat ggatcgagtt gtgatatatg gtctcttgga tgtataggat atgaattata tgttgggcat ccgttgttcc caggtaaaga caacatggat caaataggaa gaattcatgg gctatttgga tccttaccta tgttcatgct cgagcacgga aagaactcga gcaccttctt tgaaaaagaa aacggataca gattcatggg accaccatcg aactttactt tagaggatat gaaaaaaatg atcaggtcga aaggcaattc aaaggaagat gataacatgc tcataaagtt tctactgaga gcactgcagc ccagtcatct cataaggcca gatgccaaat cactagcaag ccactcttat cttaaggtca gggacacatc cgctgaagcc gataaggcct gtaacgatcg tagtggagtc cagcagaata ttttccctgc aaacaaaaac atgaggcaca tgtctacaac aggagtaatt cttcctagta agaaacaaaa gcccaccgat gacaggagga aaatctcggt ttatggaatt tcgtatgaga acaatctaaa tagagactca gaaaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa