EncuA.01475.a

CHOLINE PHOSPHATE CYTIDYLYLTRANSFERASE

CENTER ID: EncuA.01475.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
EncuA.01475.a.A1.GE27848 Full length( EncuA.01475.a ) 1 278
EncuA.01475.a.MB1.GE30462 Full length( EncuA.01475.a ) 1 278
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU10_1590
RefSeq: NP_586276.1
UniProt: Q8SR03

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKDDASEEY RMMMPSYPDR NAFEYEDMTT DVGMYRVPVN RPVRIYCDGV YDLFHYGHAR SLKQAKNLFP NVYLLVGVTD DDITVRLKGN LVMDENERAE GLIHCRYVDE VITSAPWELT LEFLQKHKID FVAHDDIPYK GEDKDDIYKF VKDMGMFIPI RRTKGISTSG IITSIVKNYD MYVRRNLERG VSAKDLNISF LQMKRIKMSK KMNEMIKNVD IQREIDDIRR EIRIAMRYWE RVSNEIISGF IENFYHGKSS NRFIDRLVGL VKLKKENA
NT Sequence
ATGAAGAAGG ATGACGCCTC AGAGGAGTAC AGGATGATGA TGCCCTCGTA TCCTGATAGG AACGCCTTTG AGTACGAGGA TATGACCACC GATGTCGGGA TGTACAGGGT GCCTGTGAAT AGACCTGTCA GGATATACTG TGATGGGGTG TACGACCTAT TTCATTATGG GCATGCGAGG AGTTTGAAGC AAGCTAAGAA TCTTTTTCCA AATGTCTATC TACTAGTTGG GGTTACTGAT GACGACATAA CGGTGAGGCT CAAGGGAAAC CTTGTTATGG ATGAGAACGA AAGGGCAGAG GGGCTGATCC ACTGCAGGTA TGTCGACGAG GTTATAACAA GCGCGCCATG GGAACTGACC CTGGAATTTC TGCAGAAGCA CAAGATAGAC TTTGTTGCGC ATGACGACAT CCCCTATAAA GGAGAGGACA AGGACGATAT CTACAAGTTT GTGAAGGACA TGGGGATGTT TATACCGATC AGAAGAACAA AGGGGATCAG TACAAGTGGG ATAATAACAA GTATTGTGAA GAACTATGAC ATGTATGTCA GGAGAAATCT TGAAAGAGGA GTTTCTGCAA AGGACCTGAA CATATCATTT CTCCAGATGA AAAGAATAAA GATGAGCAAG AAAATGAATG AAATGATCAA GAATGTTGAC ATTCAGAGGG AAATAGACGA CATAAGGAGG GAGATACGAA TTGCAATGAG ATACTGGGAA AGGGTGTCGA ACGAGATTAT CTCTGGGTTT ATAGAAAACT TCTATCATGG AAAAAGCTCC AACAGATTCA TTGACCGATT GGTGGGGCTT GTGAAGCTTA AGAAAGAGAA TGCA
Details for EncuA.01475.a.A1.GE27848
HARVESTED ON: 11/3/2009
SEQUENCED ON: 11/10/2009
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKKDDASEE YRMMMPSYPD RNAFEYEDMT TDVGMYRVPV NRPVRIYCDG VYDLFHYGHA RSLKQAKNLF PNVYLLVGVT DDDITVRLKG NLVMDENERA EGLIHCRYVD EVITSAPWEL TLEFLQKHKI DFVAHDDIPY KGEDKDDIYK FVKDMGMFIP IRRTKGISTS GIITSIVKNY DMYVRRNLER GVSAKDLNIS FLQMKRIKMS KKMNEMIKNV DIQREIDDIR REIRIAMRYW ERVSNEIISG FIENFYHGKS SNRFIDRLVG LVKLKKE
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaga aggatgacgc ctcagaggag tacaggatga tgatgccctc gtatcctgat aggaacgcct ttgagtacga ggatatgacc accgatgtcg ggatgtacag ggtgcctgtg aatagacctg tcaggatata ctgtgatggg gtgtacgacc tatttcatta tgggcatgcg aggagtttga agcaagctaa gaatcttttt ccaaatgtct atctactagt tggggttact gatgacgaca taacggtgag gctcaaggga aaccttgtta tggatgagaa cgaaagggca gaggggctga tccactgcag gtatgtcgac gaggttataa caagcgcgcc atgggaactg accctggaat ttctgcagaa gcacaagata gactttgttg cgcatgacga catcccctat aaaggagagg acaaggacga tatctacaag tttgtgaagg acatggggat gtttataccg atcagaagaa caaaggggat cagtacaagt gggataataa caagtattgt gaagaactat gacatgtatg tcaggagaaa tcttgaaaga ggagtttctg caaaggacct gaacatatca tttctccaga tgaaaagaat aaagatgagc aagaaaatga atgaaatgat caagaatgtt gacattcaga gggaaataga cgacataagg agggagatac gaattgcaat gagatactgg gaaagggtgt cgaacgagat tatctctggg tttatagaaa acttctatca tggaaaaagc tccaacagat tcattgaccg attggtgggg cttgtgaagc ttaagaaaga ga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKKDDASEE YRMMMPSYPD RNAFEYEDMT TDVGMYRVPV NRPVRIYCDG VYDLFHYGHA RSLKQAKNLF PNVYLLVGVT DDDITVRLKG NLVMDENERA EGLIHCRYVD EVITSAPWEL TLEFLQKHKI DFVAHDDIPY KGEDKDDIYK FVKDMGMFIP IRRTKGISTS GIITSIVKNY DMYVRRNLER GVSAKDLNIS FLQMKRIKMS KKMNEMIKNV DIQREIDDIR REIRIAMRYW ERVSNEIISG FIENFYHGKS SNRFIDRLVG LVKLKKENA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagaaggat gacgcctcag aggagtacag gatgatgatg ccctcgtatc ctgataggaa cgcctttgag tacgaggata tgaccaccga tgtcgggatg tacagggtgc ctgtgaatag acctgtcagg atatactgtg atggggtgta cgacctattt cattatgggc atgcgaggag tttgaagcaa gctaagaatc tttttccaaa tgtctatcta ctagttgggg ttactgatga cgacataacg gtgaggctca agggaaacct tgttatggat gagaacgaaa gggcagaggg gctgatccac tgcaggtatg tcgacgaggt tataacaagc gcgccatggg aactgaccct ggaatttctg cagaagcaca agatagactt tgttgcgcat gacgacatcc cctataaagg agaggacaag gacgatatct acaagtttgt gaaggacatg gggatgttta taccgatcag aagaacaaag gggatcagta caagtgggat aataacaagt attgtgaaga actatgacat gtatgtcagg agaaatcttg aaagaggagt ttctgcaaag gacctgaaca tatcatttct ccagatgaaa agaataaaga tgagcaagaa aatgaatgaa atgatcaaga atgttgacat tcagagggaa atagacgaca taaggaggga gatacgaatt gcaatgagat actgggaaag ggtgtcgaac gagattatct ctgggtttat agaaaacttc tatcatggaa aaagctccaa cagattcatt gaccgattgg tggggcttgt gaagcttaag aaagagaatg caaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for EncuA.01475.a.MB1.GE30462
HARVESTED ON: 5/11/2010
SEQUENCED ON: 10/7/2010
EXPECTED MW: 77kDa
OBSERVED MW: 75kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 83
PERCENT COVERAGE: 44
Validated AA Sequence
MKKDDASEEY RMMMPSYPDR NAFEYEDMTT DVGMYRVPVN RPVRIYCDGV YDLFHYGHAR SLKQAKNLFP NVYLLVGVTD DDITVRLKGN LVMDENERAE GLIHCRYVDX VITSAPWELT LEFLQKHKID FVAHDDXPYK GEDKDDIYXF VKDMGMFIPI RXTKGIXTSG IITSIVKNYD MYVXRNLERX VSAXDLNISX LXMXRIKMSK KMNXMIXNXD IXREXDXIXX XIXXAMRYXE RXXXEXXSXV IEXFYXXXSS XXFIXRXXGX XXLXKXXXXX XAXXILXXXL XXXXXAXXXX XXXXXXLXP
Validated NT Sequence
gnnnnnnnnn nnnncnngnn ctcagaccca gggtcctggt tcgatgaaga aggatgacgc ctcagaggag tacaggatga tgatgccctc gtatcctgat aggaacgcct ttgagtacga ggatatgacc accgatgtcg ggatgtacag ggtgcctgtg aatagacctg tcaggatata ctgtgatggg gtgtacgacc tatttcatta tgggcatgcg aggagtttga agcaagctaa gaatcttttt ccaaatgtct atctactagt tggggttact gatgacgaca taacggtgag gctcaaggga aaccttgtta tggatgagaa cgaaagggca gaggggctga tccactgcag gtatgtcgac naggttataa caagcgcgcc atgggaactg accctggaat ttctgcagaa gcacaagata gactttgttg cgcatgacga cntcccctat aaaggagagg acaaggacga tatctacnag tttgtgaagg acatggggat gtttataccg atcagaanaa caaaggggat cantacaagt gggataataa caagtattgt gaagaactat gacatgtatg tcangagaaa tcttgaaaga ngagtttctg cnnaggacct gaacatatca nttctccnga tgaanagaat aaagatgagc aagaaaatga atganatgat cnanaatgnt gacattcnna gggaaanaga cnacatangg ngggnnatac nnantgcnat gagatactgn gaaagggnnn cnnncgagat nntctctngg gttatagaan acttctatna ngnnnaaagc tcccncnnat tcattgancg annggngggg nnngngnngc tnnngaaana naangnnnan naaanagcnc nnncnattct gnngncnnnc cttcnnnnnn nnnnnnctgc nnncanannc cnnaanaann nnnnttgnnn gctgnnnccn c
Expected Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS MKKDDASEEY RMMMPSYPDR NAFEYEDMTT DVGMYRVPVN RPVRIYCDGV YDLFHYGHAR SLKQAKNLFP NVYLLVGVTD DDITVRLKGN LVMDENERAE GLIHCRYVDE VITSAPWELT LEFLQKHKID FVAHDDIPYK GEDKDDIYKF VKDMGMFIPI RRTKGISTSG IITSIVKNYD MYVRRNLERG VSAKDLNISF LQMKRIKMSK KMNEMIKNVD IQREIDDIRR EIRIAMRYWE RVSNEIISGF IENFYHGKSS NRFIDRLVGL VKLKKENA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcgatgaa gaaggatgac gcctcagagg agtacaggat gatgatgccc tcgtatcctg ataggaacgc ctttgagtac gaggatatga ccaccgatgt cgggatgtac agggtgcctg tgaatagacc tgtcaggata tactgtgatg gggtgtacga cctatttcat tatgggcatg cgaggagttt gaagcaagct aagaatcttt ttccaaatgt ctatctacta gttggggtta ctgatgacga cataacggtg aggctcaagg gaaaccttgt tatggatgag aacgaaaggg cagaggggct gatccactgc aggtatgtcg acgaggttat aacaagcgcg ccatgggaac tgaccctgga atttctgcag aagcacaaga tagactttgt tgcgcatgac gacatcccct ataaaggaga ggacaaggac gatatctaca agtttgtgaa ggacatgggg atgtttatac cgatcagaag aacaaagggg atcagtacaa gtgggataat aacaagtatt gtgaagaact atgacatgta tgtcaggaga aatcttgaaa gaggagtttc tgcaaaggac ctgaacatat catttctcca gatgaaaaga ataaagatga gcaagaaaat gaatgaaatg atcaagaatg ttgacattca gagggaaata gacgacataa ggagggagat acgaattgca atgagatact gggaaagggt gtcgaacgag attatctctg ggtttataga aaacttctat catggaaaaa gctccaacag attcattgac cgattggtgg ggcttgtgaa gcttaagaaa gagaatgcat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa