EncuA.01470.a

HEAT SHOCK TRANSCRIPTION FACTOR HSF

CENTER ID: EncuA.01470.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.01470.a.A1.GE27852 Full length( EncuA.01470.a ) 1 295
EncuA.01470.a.A1.GE28359 Full length( EncuA.01470.a ) 1 295
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.01470.a.A1.PS00522 Full length( EncuA.01470.a ) 1 295

External Resources

RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU08_0970
RefSeq: NP_597227.1
UniProt: Q8SRB4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMSMRLATGS EFVVKLFQIL EEPENYPYIS WMPDGRSFVI SDQQGFSEFV LEKHFRHKNW SSFVRQLNKY DFYKVRRDGE RTGCIEAMWE YRNKHFQRGK HELLSKIRRK KAPSEGCGAG GTNPKQIESG IVYQSHVLDT VRSISKYLQT VVEDINEIKK YIYHERMRIA LKTSVLFVRE GVPGSEWVYG ALKIPNYSLT VVEESADVGS IMRWRYDVVV LYANSPRCIT AVSRIRDEDE EVPIVLVVDE GFKSEYANIL GIRATEALLM PFDPSELVDA INRHGSQRSP EGVHG
NT Sequence
ATGATGTCTA TGCGATTGGC GACGGGGTCT GAGTTTGTGG TGAAGCTTTT CCAGATACTC GAGGAGCCCG AGAACTATCC GTACATATCA TGGATGCCCG ATGGAAGAAG CTTTGTCATA TCTGACCAGC AGGGCTTTTC TGAGTTTGTT CTTGAGAAAC ACTTCAGGCA CAAGAACTGG AGTTCCTTTG TCAGGCAGCT CAACAAGTAC GACTTCTACA AGGTACGGAG AGATGGAGAA AGGACCGGGT GCATTGAGGC TATGTGGGAG TACAGGAACA AGCATTTCCA ACGGGGGAAG CACGAGCTGC TGAGCAAGAT CAGGAGGAAG AAGGCGCCGA GCGAAGGGTG TGGGGCTGGA GGAACAAACC CAAAGCAGAT TGAAAGCGGA ATAGTCTACC AATCGCACGT GCTTGACACT GTGCGGAGTA TTTCCAAGTA TCTTCAAACT GTTGTTGAGG ACATCAACGA GATAAAGAAA TACATTTACC ACGAAAGAAT GAGAATTGCT TTGAAGACAA GTGTTCTGTT TGTCAGGGAG GGTGTTCCTG GGTCTGAATG GGTCTACGGA GCCCTAAAGA TTCCAAACTA CTCTCTGACC GTGGTGGAGG AGAGCGCCGA TGTGGGAAGC ATCATGAGAT GGAGGTACGA TGTAGTTGTG TTGTATGCCA ACTCGCCCCG ATGCATTACG GCTGTGAGCC GAATAAGGGA CGAAGACGAG GAGGTTCCGA TTGTTCTGGT TGTCGACGAA GGATTCAAGA GCGAGTATGC GAACATTCTG GGGATTAGAG CTACGGAGGC CCTTCTAATG CCTTTTGACC CAAGTGAGCT GGTTGACGCC ATCAACAGGC ATGGGAGTCA AAGAAGCCCG GAAGGAGTCC ATGGC
Details for EncuA.01470.a.A1.GE27852
HARVESTED ON: 11/3/2009
SEQUENCED ON: 11/10/2009
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMMSMRLATG SEFVVKLFQI LEEPENYPYI SWMPDGRSFV ISDQQGFSEF VLEKHFRHKN WSSFVRQLNK YDFYKVRRDG ERTGCIEAMW EYRNKHFQRG KHELLSKIRR KKAPSEGCGA GGTNPKQIES GIVYQSHVLD TVRSISKYLQ TVVEDINEIK KYIYHERMRI ALKTSVLFVR EGVPGSEWVY GALKIPNYSL TVVEESADVG SIMRWRYDVV VLYANSPRCI TAVSRIRDED EEVPIVLVVD EGFKSEYANI LGIRATEALL MPFDPSELVD AINRHGSQRS PEG
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgatgt ctatgcgatt ggcgacgggg tctgagtttg tggtgaagct tttccagata ctcgaggagc ccgagaacta tccgtacata tcatggatgc ccgatggaag aagctttgtc atatctgacc agcagggctt ttctgagttt gttcttgaga aacacttcag gcacaagaac tggagttcct ttgtcaggca gctcaacaag tacgacttct acaaggtacg gagagatgga gaaaggaccg ggtgcattga ggctatgtgg gagtacagga acaagcattt ccaacggggg aagcacgagc tgctgagcaa gatcaggagg aagaaggcgc cgagcgaagg gtgtggggct ggaggaacaa acccaaagca gattgaaagc ggaatagtct accaatcgca cgtgcttgac actgtgcgga gtatttccaa gtatcttcaa actgttgttg aggacatcaa cgagataaag aaatacattt accacgaaag aatgagaatt gctttgaaga caagtgttct gtttgtcagg gagggtgttc ctgggtctga atgggtctac ggagccctaa agattccaaa ctactctctg accgtggtgg aggagagcgc cgatgtggga agcatcatga gatggaggta cgatgtagtt gtgttgtatg ccaactcgcc ccgatgcatt acggctgtga gccgaataag ggacgaagac gaggaggttc cgattgttct ggttgtcgac gaaggattca agagcgagta tgcgaacatt ctggggatta gagctacgga ggcccttcta atgccttttg acccaagtga gctggttgac gccatcaaca ggcatgggag tcaaagaagc ccggaaggag t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMMSMRLATG SEFVVKLFQI LEEPENYPYI SWMPDGRSFV ISDQQGFSEF VLEKHFRHKN WSSFVRQLNK YDFYKVRRDG ERTGCIEAMW EYRNKHFQRG KHELLSKIRR KKAPSEGCGA GGTNPKQIES GIVYQSHVLD TVRSISKYLQ TVVEDINEIK KYIYHERMRI ALKTSVLFVR EGVPGSEWVY GALKIPNYSL TVVEESADVG SIMRWRYDVV VLYANSPRCI TAVSRIRDED EEVPIVLVVD EGFKSEYANI LGIRATEALL MPFDPSELVD AINRHGSQRS PEGVHG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatgtctatg cgattggcga cggggtctga gtttgtggtg aagcttttcc agatactcga ggagcccgag aactatccgt acatatcatg gatgcccgat ggaagaagct ttgtcatatc tgaccagcag ggcttttctg agtttgttct tgagaaacac ttcaggcaca agaactggag ttcctttgtc aggcagctca acaagtacga cttctacaag gtacggagag atggagaaag gaccgggtgc attgaggcta tgtgggagta caggaacaag catttccaac gggggaagca cgagctgctg agcaagatca ggaggaagaa ggcgccgagc gaagggtgtg gggctggagg aacaaaccca aagcagattg aaagcggaat agtctaccaa tcgcacgtgc ttgacactgt gcggagtatt tccaagtatc ttcaaactgt tgttgaggac atcaacgaga taaagaaata catttaccac gaaagaatga gaattgcttt gaagacaagt gttctgtttg tcagggaggg tgttcctggg tctgaatggg tctacggagc cctaaagatt ccaaactact ctctgaccgt ggtggaggag agcgccgatg tgggaagcat catgagatgg aggtacgatg tagttgtgtt gtatgccaac tcgccccgat gcattacggc tgtgagccga ataagggacg aagacgagga ggttccgatt gttctggttg tcgacgaagg attcaagagc gagtatgcga acattctggg gattagagct acggaggccc ttctaatgcc ttttgaccca agtgagctgg ttgacgccat caacaggcat gggagtcaaa gaagcccgga aggagtccat ggcaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for EncuA.01470.a.A1.GE28359
HARVESTED ON: 1/5/2010
SEQUENCED ON: 1/15/2010
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMMSMRLATG SEFVVKLFQI LEEPENYPYI SWMPDGRSFV ISDQQGFSEF VLEKHFRHKN WSSFVRQLNK YDFYKVRRDG ERTGCIEAMW EYRNKHFQRG KHELLSKIRR KKAPSEGCGA GGTNPKQIES GIVYQSHVLD TVRSISKYLQ TVVEDINEIK KYIYHERMRI ALKTSVLFVR EGVPGSEWVY GALKIPNYSL TVVEESADVG SIMRWRYDVV VLYANSPRCI TAVSRIRDED EEVPIVLVVD EGFKSEYANI LGIRATEALL MPFDPSELVD AINRHGSQRS PEG
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgatgt ctatgcgatt ggcgacgggg tctgagtttg tggtgaagct tttccagata ctcgaggagc ccgagaacta tccgtacata tcatggatgc ccgatggaag aagctttgtc atatctgacc agcagggctt ttctgagttt gttcttgaga aacacttcag gcacaagaac tggagttcct ttgtcaggca gctcaacaag tacgacttct acaaggtacg gagagatgga gaaaggaccg ggtgcattga ggctatgtgg gagtacagga acaagcattt ccaacggggg aagcacgagc tgctgagcaa gatcaggagg aagaaggcgc cgagcgaagg gtgtggggct ggaggaacaa acccaaagca gattgaaagc ggaatagtct accaatcgca cgtgcttgac actgtgcgga gtatttccaa gtatcttcaa actgttgttg aggacatcaa cgagataaag aaatacattt accacgaaag aatgagaatt gctttgaaga caagtgttct gtttgtcagg gagggtgttc ctgggtctga atgggtctac ggagccctaa agattccaaa ctactctctg accgtggtgg aggagagcgc cgatgtggga agcatcatga gatggaggta cgatgtagtt gtgttgtatg ccaactcgcc ccgatgcatt acggctgtga gccgaataag ggacgaagac gaggaggttc cgattgttct ggttgtcgac gaaggattca agagcgagta tgcgaacatt ctggggatta gagctacgga ggcccttcta atgccttttg acccaagtga gctggttgac gccatcaaca ggcatgggag tcaaagaagc ccggaaggag t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMMSMRLATG SEFVVKLFQI LEEPENYPYI SWMPDGRSFV ISDQQGFSEF VLEKHFRHKN WSSFVRQLNK YDFYKVRRDG ERTGCIEAMW EYRNKHFQRG KHELLSKIRR KKAPSEGCGA GGTNPKQIES GIVYQSHVLD TVRSISKYLQ TVVEDINEIK KYIYHERMRI ALKTSVLFVR EGVPGSEWVY GALKIPNYSL TVVEESADVG SIMRWRYDVV VLYANSPRCI TAVSRIRDED EEVPIVLVVD EGFKSEYANI LGIRATEALL MPFDPSELVD AINRHGSQRS PEGVHG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatgtctatg cgattggcga cggggtctga gtttgtggtg aagcttttcc agatactcga ggagcccgag aactatccgt acatatcatg gatgcccgat ggaagaagct ttgtcatatc tgaccagcag ggcttttctg agtttgttct tgagaaacac ttcaggcaca agaactggag ttcctttgtc aggcagctca acaagtacga cttctacaag gtacggagag atggagaaag gaccgggtgc attgaggcta tgtgggagta caggaacaag catttccaac gggggaagca cgagctgctg agcaagatca ggaggaagaa ggcgccgagc gaagggtgtg gggctggagg aacaaaccca aagcagattg aaagcggaat agtctaccaa tcgcacgtgc ttgacactgt gcggagtatt tccaagtatc ttcaaactgt tgttgaggac atcaacgaga taaagaaata catttaccac gaaagaatga gaattgcttt gaagacaagt gttctgtttg tcagggaggg tgttcctggg tctgaatggg tctacggagc cctaaagatt ccaaactact ctctgaccgt ggtggaggag agcgccgatg tgggaagcat catgagatgg aggtacgatg tagttgtgtt gtatgccaac tcgccccgat gcattacggc tgtgagccga ataagggacg aagacgagga ggttccgatt gttctggttg tcgacgaagg attcaagagc gagtatgcga acattctggg gattagagct acggaggccc ttctaatgcc ttttgaccca agtgagctgg ttgacgccat caacaggcat gggagtcaaa gaagcccgga aggagtccat ggcaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for EncuA.01470.a.A1.PS00522
PURIFICATION DATe: 2/17/2010
CONCENTRATION: 1.64mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMMSMRLATG SEFVVKLFQI LEEPENYPYI SWMPDGRSFV ISDQQGFSEF VLEKHFRHKN WSSFVRQLNK YDFYKVRRDG ERTGCIEAMW EYRNKHFQRG KHELLSKIRR KKAPSEGCGA GGTNPKQIES GIVYQSHVLD TVRSISKYLQ TVVEDINEIK KYIYHERMRI ALKTSVLFVR EGVPGSEWVY GALKIPNYSL TVVEESADVG SIMRWRYDVV VLYANSPRCI TAVSRIRDED EEVPIVLVVD EGFKSEYANI LGIRATEALL MPFDPSELVD AINRHGSQRS PEG
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgatgt ctatgcgatt ggcgacgggg tctgagtttg tggtgaagct tttccagata ctcgaggagc ccgagaacta tccgtacata tcatggatgc ccgatggaag aagctttgtc atatctgacc agcagggctt ttctgagttt gttcttgaga aacacttcag gcacaagaac tggagttcct ttgtcaggca gctcaacaag tacgacttct acaaggtacg gagagatgga gaaaggaccg ggtgcattga ggctatgtgg gagtacagga acaagcattt ccaacggggg aagcacgagc tgctgagcaa gatcaggagg aagaaggcgc cgagcgaagg gtgtggggct ggaggaacaa acccaaagca gattgaaagc ggaatagtct accaatcgca cgtgcttgac actgtgcgga gtatttccaa gtatcttcaa actgttgttg aggacatcaa cgagataaag aaatacattt accacgaaag aatgagaatt gctttgaaga caagtgttct gtttgtcagg gagggtgttc ctgggtctga atgggtctac ggagccctaa agattccaaa ctactctctg accgtggtgg aggagagcgc cgatgtggga agcatcatga gatggaggta cgatgtagtt gtgttgtatg ccaactcgcc ccgatgcatt acggctgtga gccgaataag ggacgaagac gaggaggttc cgattgttct ggttgtcgac gaaggattca agagcgagta tgcgaacatt ctggggatta gagctacgga ggcccttcta atgccttttg acccaagtga gctggttgac gccatcaaca ggcatgggag tcaaagaagc ccggaaggag t
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMMSMRLATG SEFVVKLFQI LEEPENYPYI SWMPDGRSFV ISDQQGFSEF VLEKHFRHKN WSSFVRQLNK YDFYKVRRDG ERTGCIEAMW EYRNKHFQRG KHELLSKIRR KKAPSEGCGA GGTNPKQIES GIVYQSHVLD TVRSISKYLQ TVVEDINEIK KYIYHERMRI ALKTSVLFVR EGVPGSEWVY GALKIPNYSL TVVEESADVG SIMRWRYDVV VLYANSPRCI TAVSRIRDED EEVPIVLVVD EGFKSEYANI LGIRATEALL MPFDPSELVD AINRHGSQRS PEGVHG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GATGTCTATG CGATTGGCGA CGGGGTCTGA GTTTGTGGTG AAGCTTTTCC AGATACTCGA GGAGCCCGAG AACTATCCGT ACATATCATG GATGCCCGAT GGAAGAAGCT TTGTCATATC TGACCAGCAG GGCTTTTCTG AGTTTGTTCT TGAGAAACAC TTCAGGCACA AGAACTGGAG TTCCTTTGTC AGGCAGCTCA ACAAGTACGA CTTCTACAAG GTACGGAGAG ATGGAGAAAG GACCGGGTGC ATTGAGGCTA TGTGGGAGTA CAGGAACAAG CATTTCCAAC GGGGGAAGCA CGAGCTGCTG AGCAAGATCA GGAGGAAGAA GGCGCCGAGC GAAGGGTGTG GGGCTGGAGG AACAAACCCA AAGCAGATTG AAAGCGGAAT AGTCTACCAA TCGCACGTGC TTGACACTGT GCGGAGTATT TCCAAGTATC TTCAAACTGT TGTTGAGGAC ATCAACGAGA TAAAGAAATA CATTTACCAC GAAAGAATGA GAATTGCTTT GAAGACAAGT GTTCTGTTTG TCAGGGAGGG TGTTCCTGGG TCTGAATGGG TCTACGGAGC CCTAAAGATT CCAAACTACT CTCTGACCGT GGTGGAGGAG AGCGCCGATG TGGGAAGCAT CATGAGATGG AGGTACGATG TAGTTGTGTT GTATGCCAAC TCGCCCCGAT GCATTACGGC TGTGAGCCGA ATAAGGGACG AAGACGAGGA GGTTCCGATT GTTCTGGTTG TCGACGAAGG ATTCAAGAGC GAGTATGCGA ACATTCTGGG GATTAGAGCT ACGGAGGCCC TTCTAATGCC TTTTGACCCA AGTGAGCTGG TTGACGCCAT CAACAGGCAT GGGAGTCAAA GAAGCCCGGA AGGAGTCCAT GGCAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA