EncuA.01451.a

MRK1-LIKE SER/THR PROTEIN KINASE

CENTER ID: EncuA.01451.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.01451.a.A1.GE27847 Full length( EncuA.01451.a ) 1 334
EncuA.01451.a.A1.GE28358 Full length( EncuA.01451.a ) 1 334
EncuA.01451.a.A1.GE29796 Full length( EncuA.01451.a ) 1 334
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU07_1270
RefSeq: NP_586056.1
UniProt: Q8SRI3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MESDGFPSRT NFGLPQMATL SQKKLMESVR LMEREWVKMH KALVRSRCGE VKEVRYRYVE MIGRGSFGVV VKIMDDRHNF FALKRVYQDR RYHNRELGIL MEVDHPNIVR LVSYFHTDKT SSGAYLNIIT DFVGMNLEEY IKANRGVETE EIRSVYRQIL EGLRYLHEKN ICHRDMKPSN ILIDTNGLVK ICDLGSAKVI KSGERNITYI CSRFYRAPEN LLDYKEYDFK IDIWSVGCVI AEFRHPGPIF KGDTSGSTLN RILEIVRVTS DDLIGLGCLK PDLKQGVGIR KYLEAFFSDP DLLEVLEKSL AFSPCKRSTA SELLRKQFFQ QAHEX
NT Sequence
ATGGAGTCTG ATGGATTCCC TTCAAGAACG AATTTCGGGC TTCCCCAAAT GGCAACATTG TCCCAGAAGA AGCTTATGGA GTCCGTAAGG CTGATGGAGA GGGAATGGGT AAAGATGCAC AAAGCCCTGG TGCGGTCGAG ATGCGGGGAG GTCAAGGAGG TTAGATACAG ATATGTCGAG ATGATTGGAA GAGGGTCTTT TGGCGTGGTC GTCAAGATAA TGGACGACAG ACATAACTTC TTTGCGCTGA AGAGGGTTTA CCAAGATAGA AGATACCACA ATAGGGAGCT TGGGATTCTG ATGGAGGTGG ATCATCCAAA CATTGTGAGG CTTGTGTCGT ACTTCCACAC TGACAAAACA TCTTCTGGCG CATATCTCAA CATCATCACA GACTTTGTTG GGATGAACCT GGAGGAATAC ATAAAGGCAA ATAGAGGCGT GGAGACAGAG GAGATCAGGT CGGTATACAG GCAGATTCTG GAGGGGCTGA GATATCTGCA TGAGAAGAAC ATATGCCATA GAGATATGAA GCCGTCAAAC ATACTCATAG ACACCAACGG ACTGGTCAAG ATCTGCGACC TTGGAAGTGC GAAGGTAATC AAGAGTGGAG AAAGGAACAT AACATATATA TGCTCCAGGT TCTACCGGGC GCCGGAGAAT CTCCTCGACT ACAAGGAATA TGACTTCAAG ATCGACATAT GGTCGGTTGG ATGTGTGATT GCCGAATTCA GGCACCCCGG GCCTATATTT AAGGGAGACA CCAGCGGATC TACGCTCAAC AGGATCCTCG AGATTGTTAG AGTCACTTCG GACGACCTGA TTGGGCTTGG GTGTCTAAAA CCCGACCTTA AGCAGGGGGT AGGCATCAGA AAGTATCTTG AGGCATTCTT CAGTGATCCC GATCTTCTGG AGGTTCTTGA AAAGTCCCTG GCATTTTCTC CCTGCAAAAG GTCCACAGCC TCGGAGCTAC TGAGAAAGCA GTTCTTCCAG CAGGCACATG AG
Details for EncuA.01451.a.A1.GE27847
HARVESTED ON: 11/3/2009
SEQUENCED ON: 11/10/2009
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMESDGFPSR TNFGLPQMAT LSQKKLMESV RLMEREWVKM HKALVRSRCG EVKEVRYRYV EMIGRGSFGV VVKIMDDRHN FFALKRVYQD RRYHNRELGI LMEVDHPNIV RLVSYFHTDK TSSGAYLNII TDFVGMNLEE YIKANRGVET EEIRSVYRQI LEGLRYLHEK NICHRDMKPS NILIDTNGLV KICDLGSAKV IKSGERNITY ICSRFYRAPE NLLDYKEYDF KIDIWSVGCV IAEFRHPGPI FKGDTSGSTL NRILEIVRVT SDDLIGLGCL KPDLKQGVGI RKYLEAFFSD PDLLEVLEKS LAFSPCKRST ASELLRKQFF QQ
Validated NT Sequence
tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggagtc tgatggattc ccttcaagaa cgaatttcgg gcttccccaa atggcaacat tgtcccagaa gaagcttatg gagtccgtaa ggctgatgga gagggaatgg gtaaagatgc acaaagccct ggtgcggtcg agatgcgggg aggtcaagga ggttagatac agatatgtcg agatgattgg aagagggtct tttggcgtgg tcgtcaagat aatggacgac agacataact tctttgcgct gaagagggtt taccaagata gaagatacca caatagggag cttgggattc tgatggaggt ggatcatcca aacattgtga ggcttgtgtc gtacttccac actgacaaaa catcttctgg cgcatatctc aacatcatca cagactttgt tgggatgaac ctggaggaat acataaaggc aaatagaggc gtggagacag aggagatcag gtcggtatac aggcagattc tggaggggct gagatatctg catgagaaga acatatgcca tagagatatg aagccgtcaa acatactcat agacaccaac ggactggtca agatctgcga ccttggaagt gcgaaggtaa tcaagagtgg agaaaggaac ataacatata tatgctccag gttctaccgg gcgccggaga atctcctcga ctacaaggaa tatgacttca agatcgacat atggtcggtt ggatgtgtga ttgccgaatt caggcacccc gggcctatat ttaagggaga caccagcgga tctacgctca acaggatcct cgagattgtt agagtcactt cggacgacct gattgggctt gggtgtctaa aacccgacct taagcagggg gtaggcatca gaaagtatct tgaggcattc ttcagtgatc ccgatcttct ggaggttctt gaaaagtccc tggcattttc tccctgcaaa aggtccacag cctcggagct actgagaaag cagttcttcc agcaggca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMESDGFPSR TNFGLPQMAT LSQKKLMESV RLMEREWVKM HKALVRSRCG EVKEVRYRYV EMIGRGSFGV VVKIMDDRHN FFALKRVYQD RRYHNRELGI LMEVDHPNIV RLVSYFHTDK TSSGAYLNII TDFVGMNLEE YIKANRGVET EEIRSVYRQI LEGLRYLHEK NICHRDMKPS NILIDTNGLV KICDLGSAKV IKSGERNITY ICSRFYRAPE NLLDYKEYDF KIDIWSVGCV IAEFRHPGPI FKGDTSGSTL NRILEIVRVT SDDLIGLGCL KPDLKQGVGI RKYLEAFFSD PDLLEVLEKS LAFSPCKRST ASELLRKQFF QQAHE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggagtctgat ggattccctt caagaacgaa tttcgggctt ccccaaatgg caacattgtc ccagaagaag cttatggagt ccgtaaggct gatggagagg gaatgggtaa agatgcacaa agccctggtg cggtcgagat gcggggaggt caaggaggtt agatacagat atgtcgagat gattggaaga gggtcttttg gcgtggtcgt caagataatg gacgacagac ataacttctt tgcgctgaag agggtttacc aagatagaag ataccacaat agggagcttg ggattctgat ggaggtggat catccaaaca ttgtgaggct tgtgtcgtac ttccacactg acaaaacatc ttctggcgca tatctcaaca tcatcacaga ctttgttggg atgaacctgg aggaatacat aaaggcaaat agaggcgtgg agacagagga gatcaggtcg gtatacaggc agattctgga ggggctgaga tatctgcatg agaagaacat atgccataga gatatgaagc cgtcaaacat actcatagac accaacggac tggtcaagat ctgcgacctt ggaagtgcga aggtaatcaa gagtggagaa aggaacataa catatatatg ctccaggttc taccgggcgc cggagaatct cctcgactac aaggaatatg acttcaagat cgacatatgg tcggttggat gtgtgattgc cgaattcagg caccccgggc ctatatttaa gggagacacc agcggatcta cgctcaacag gatcctcgag attgttagag tcacttcgga cgacctgatt gggcttgggt gtctaaaacc cgaccttaag cagggggtag gcatcagaaa gtatcttgag gcattcttca gtgatcccga tcttctggag gttcttgaaa agtccctggc attttctccc tgcaaaaggt ccacagcctc ggagctactg agaaagcagt tcttccagca ggcacatgag aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for EncuA.01451.a.A1.GE28358
HARVESTED ON: 1/5/2010
SEQUENCED ON: 1/15/2010
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMESDGFPSR TNFGLPQMAT LSQKKLMESV RLMEREWVKM HKALVRSRCG EVKEVRYRYV EMIGRGSFGV VVKIMDDRHN FFALKRVYQD RRYHNRELGI LMEVDHPNIV RLVSYFHTDK TSSGAYLNII TDFVGMNLEE YIKANRGVET EEIRSVYRQI LEGLRYLHEK NICHRDMKPS NILIDTNGLV KICDLGSAKV IKSGERNITY ICSRFYRAPE NLLDYKEYDF KIDIWSVGCV IAEFRHPGPI FKGDTSGSTL NRILEIVRVT SDDLIGLGCL KPDLKQGVGI RKYLEAFFSD PDLLEVLEKS LAFSPCKRST ASELLRKQFF QQ
Validated NT Sequence
tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggagtc tgatggattc ccttcaagaa cgaatttcgg gcttccccaa atggcaacat tgtcccagaa gaagcttatg gagtccgtaa ggctgatgga gagggaatgg gtaaagatgc acaaagccct ggtgcggtcg agatgcgggg aggtcaagga ggttagatac agatatgtcg agatgattgg aagagggtct tttggcgtgg tcgtcaagat aatggacgac agacataact tctttgcgct gaagagggtt taccaagata gaagatacca caatagggag cttgggattc tgatggaggt ggatcatcca aacattgtga ggcttgtgtc gtacttccac actgacaaaa catcttctgg cgcatatctc aacatcatca cagactttgt tgggatgaac ctggaggaat acataaaggc aaatagaggc gtggagacag aggagatcag gtcggtatac aggcagattc tggaggggct gagatatctg catgagaaga acatatgcca tagagatatg aagccgtcaa acatactcat agacaccaac ggactggtca agatctgcga ccttggaagt gcgaaggtaa tcaagagtgg agaaaggaac ataacatata tatgctccag gttctaccgg gcgccggaga atctcctcga ctacaaggaa tatgacttca agatcgacat atggtcggtt ggatgtgtga ttgccgaatt caggcacccc gggcctatat ttaagggaga caccagcgga tctacgctca acaggatcct cgagattgtt agagtcactt cggacgacct gattgggctt gggtgtctaa aacccgacct taagcagggg gtaggcatca gaaagtatct tgaggcattc ttcagtgatc ccgatcttct ggaggttctt gaaaagtccc tggcattttc tccctgcaaa aggtccacag cctcggagct actgagaaag cagttcttcc agcaggca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMESDGFPSR TNFGLPQMAT LSQKKLMESV RLMEREWVKM HKALVRSRCG EVKEVRYRYV EMIGRGSFGV VVKIMDDRHN FFALKRVYQD RRYHNRELGI LMEVDHPNIV RLVSYFHTDK TSSGAYLNII TDFVGMNLEE YIKANRGVET EEIRSVYRQI LEGLRYLHEK NICHRDMKPS NILIDTNGLV KICDLGSAKV IKSGERNITY ICSRFYRAPE NLLDYKEYDF KIDIWSVGCV IAEFRHPGPI FKGDTSGSTL NRILEIVRVT SDDLIGLGCL KPDLKQGVGI RKYLEAFFSD PDLLEVLEKS LAFSPCKRST ASELLRKQFF QQAHE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggagtctgat ggattccctt caagaacgaa tttcgggctt ccccaaatgg caacattgtc ccagaagaag cttatggagt ccgtaaggct gatggagagg gaatgggtaa agatgcacaa agccctggtg cggtcgagat gcggggaggt caaggaggtt agatacagat atgtcgagat gattggaaga gggtcttttg gcgtggtcgt caagataatg gacgacagac ataacttctt tgcgctgaag agggtttacc aagatagaag ataccacaat agggagcttg ggattctgat ggaggtggat catccaaaca ttgtgaggct tgtgtcgtac ttccacactg acaaaacatc ttctggcgca tatctcaaca tcatcacaga ctttgttggg atgaacctgg aggaatacat aaaggcaaat agaggcgtgg agacagagga gatcaggtcg gtatacaggc agattctgga ggggctgaga tatctgcatg agaagaacat atgccataga gatatgaagc cgtcaaacat actcatagac accaacggac tggtcaagat ctgcgacctt ggaagtgcga aggtaatcaa gagtggagaa aggaacataa catatatatg ctccaggttc taccgggcgc cggagaatct cctcgactac aaggaatatg acttcaagat cgacatatgg tcggttggat gtgtgattgc cgaattcagg caccccgggc ctatatttaa gggagacacc agcggatcta cgctcaacag gatcctcgag attgttagag tcacttcgga cgacctgatt gggcttgggt gtctaaaacc cgaccttaag cagggggtag gcatcagaaa gtatcttgag gcattcttca gtgatcccga tcttctggag gttcttgaaa agtccctggc attttctccc tgcaaaaggt ccacagcctc ggagctactg agaaagcagt tcttccagca ggcacatgag aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for EncuA.01451.a.A1.GE29796
HARVESTED ON: 5/27/2010
SEQUENCED ON: 8/31/2010
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMESDGFPSR TNFGLPQMAT LSQKKLMESV RLMEREWVKM HKALVRSRCG EVKEVRYRYV EMIGRGSFGV VVKIMDDRHN FFALKRVYQD RRYHNRELGI LMEVDHPNIV RLVSYFHTDK TSSGAYLNII TDFVGMNLEE YIKANRGVET EEIRSVYRQI LEGLRYLHEK NICHRDMKPS NILIDTNGLV KICDLGSAKV IKSGERNITY ICSRFYRAPE NLLDYKEYDF KIDIWSVGCV IAEFRHPGPI FKGDTSGSTL NRILEIVRVT SDDLIGLGCL KPDLKQGVGI RKYLEAFFSD PDLLEVLEKS LAFSPCKRST ASELLRKQFF QQ
Validated NT Sequence
tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggagtc tgatggattc ccttcaagaa cgaatttcgg gcttccccaa atggcaacat tgtcccagaa gaagcttatg gagtccgtaa ggctgatgga gagggaatgg gtaaagatgc acaaagccct ggtgcggtcg agatgcgggg aggtcaagga ggttagatac agatatgtcg agatgattgg aagagggtct tttggcgtgg tcgtcaagat aatggacgac agacataact tctttgcgct gaagagggtt taccaagata gaagatacca caatagggag cttgggattc tgatggaggt ggatcatcca aacattgtga ggcttgtgtc gtacttccac actgacaaaa catcttctgg cgcatatctc aacatcatca cagactttgt tgggatgaac ctggaggaat acataaaggc aaatagaggc gtggagacag aggagatcag gtcggtatac aggcagattc tggaggggct gagatatctg catgagaaga acatatgcca tagagatatg aagccgtcaa acatactcat agacaccaac ggactggtca agatctgcga ccttggaagt gcgaaggtaa tcaagagtgg agaaaggaac ataacatata tatgctccag gttctaccgg gcgccggaga atctcctcga ctacaaggaa tatgacttca agatcgacat atggtcggtt ggatgtgtga ttgccgaatt caggcacccc gggcctatat ttaagggaga caccagcgga tctacgctca acaggatcct cgagattgtt agagtcactt cggacgacct gattgggctt gggtgtctaa aacccgacct taagcagggg gtaggcatca gaaagtatct tgaggcattc ttcagtgatc ccgatcttct ggaggttctt gaaaagtccc tggcattttc tccctgcaaa aggtccacag cctcggagct actgagaaag cagttcttcc agcaggca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMESDGFPSR TNFGLPQMAT LSQKKLMESV RLMEREWVKM HKALVRSRCG EVKEVRYRYV EMIGRGSFGV VVKIMDDRHN FFALKRVYQD RRYHNRELGI LMEVDHPNIV RLVSYFHTDK TSSGAYLNII TDFVGMNLEE YIKANRGVET EEIRSVYRQI LEGLRYLHEK NICHRDMKPS NILIDTNGLV KICDLGSAKV IKSGERNITY ICSRFYRAPE NLLDYKEYDF KIDIWSVGCV IAEFRHPGPI FKGDTSGSTL NRILEIVRVT SDDLIGLGCL KPDLKQGVGI RKYLEAFFSD PDLLEVLEKS LAFSPCKRST ASELLRKQFF QQAHE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggagtctgat ggattccctt caagaacgaa tttcgggctt ccccaaatgg caacattgtc ccagaagaag cttatggagt ccgtaaggct gatggagagg gaatgggtaa agatgcacaa agccctggtg cggtcgagat gcggggaggt caaggaggtt agatacagat atgtcgagat gattggaaga gggtcttttg gcgtggtcgt caagataatg gacgacagac ataacttctt tgcgctgaag agggtttacc aagatagaag ataccacaat agggagcttg ggattctgat ggaggtggat catccaaaca ttgtgaggct tgtgtcgtac ttccacactg acaaaacatc ttctggcgca tatctcaaca tcatcacaga ctttgttggg atgaacctgg aggaatacat aaaggcaaat agaggcgtgg agacagagga gatcaggtcg gtatacaggc agattctgga ggggctgaga tatctgcatg agaagaacat atgccataga gatatgaagc cgtcaaacat actcatagac accaacggac tggtcaagat ctgcgacctt ggaagtgcga aggtaatcaa gagtggagaa aggaacataa catatatatg ctccaggttc taccgggcgc cggagaatct cctcgactac aaggaatatg acttcaagat cgacatatgg tcggttggat gtgtgattgc cgaattcagg caccccgggc ctatatttaa gggagacacc agcggatcta cgctcaacag gatcctcgag attgttagag tcacttcgga cgacctgatt gggcttgggt gtctaaaacc cgaccttaag cagggggtag gcatcagaaa gtatcttgag gcattcttca gtgatcccga tcttctggag gttcttgaaa agtccctggc attttctccc tgcaaaaggt ccacagcctc ggagctactg agaaagcagt tcttccagca ggcacatgag aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa