EncuA.01426.a

CELL CYCLE PROTEIN KINASE

CENTER ID: EncuA.01426.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
EncuA.01426.a.A1.GE27841 Full length( EncuA.01426.a ) 1 309
EncuA.01426.a.A1.GE28354 Full length( EncuA.01426.a ) 1 309
EncuA.01426.a.A1.GE29795 Full length( EncuA.01426.a ) 1 309
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU08_1790
OrthoMCL: OG5_224722
RefSeq: NP_597307.1
UniProt: Q8SR90
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEKYENIKQV GEGAFGQVYK SRRTSDGAVF AIKKMPVDRE TGFPFTAIRE IKLCKSVINK HIIGLDEIVF EEGFIFVVLE YMPYDLTGLL ASGAKLSTDQ IRSITSQLIE AVSSMHGMGL VHRDIKPSNI LLDCHGMLKL TDFGLTREIS GMMTNRVCTL WYRAPELLLG ETSYSLKVDA WSVGCIMLEM RLGRPPYRGS DEVSQIKLIF EELGIPQDKY KWSDLLDVDI YSKSRSTEEI IAERYGHLFD EEELKVLSGF LCLTSRKRLS VANSRYFTVI SSHKNTYIPL SFEEAHELYT KERKETKKP
NT Sequence
ATGGAGAAAT ACGAAAATAT CAAACAAGTA GGAGAGGGAG CGTTCGGGCA GGTTTACAAG TCCAGAAGAA CATCTGATGG GGCAGTGTTT GCAATAAAAA AGATGCCAGT GGACAGAGAG ACCGGCTTTC CATTTACAGC CATACGGGAG ATAAAGTTGT GCAAGAGCGT AATTAATAAG CACATAATAG GGCTTGACGA AATTGTGTTC GAGGAGGGCT TCATATTTGT TGTACTTGAA TACATGCCAT ATGATCTGAC GGGGCTTCTT GCATCTGGCG CCAAGCTCAG CACCGACCAG ATAAGATCGA TCACATCGCA ACTCATAGAG GCTGTGAGCT CCATGCACGG GATGGGGCTG GTTCATCGAG ACATAAAGCC CAGCAATATC CTGCTAGACT GCCACGGGAT GCTGAAGCTC ACGGACTTTG GGCTGACCAG AGAGATATCG GGGATGATGA CGAACAGAGT TTGCACACTC TGGTACAGGG CGCCTGAGCT ATTGCTTGGA GAAACATCCT ACAGCCTTAA GGTGGATGCG TGGTCTGTTG GGTGCATAAT GCTAGAAATG AGGCTTGGGA GACCGCCATA TAGAGGAAGC GATGAAGTAT CGCAGATAAA GCTGATCTTC GAGGAGCTAG GCATACCTCA GGACAAATAC AAGTGGTCTG ACCTGCTTGA CGTTGATATT TACTCCAAGA GCAGGAGTAC CGAGGAAATA ATTGCCGAGA GATACGGACA TCTGTTTGAC GAGGAAGAGC TAAAGGTTCT TTCTGGATTT CTTTGCCTAA CCAGTAGGAA GAGGTTGTCT GTGGCCAATT CTAGGTATTT CACCGTCATC TCAAGCCACA AAAACACCTA TATCCCACTG AGCTTTGAGG AAGCTCACGA GCTGTACACA AAGGAAAGGA AAGAAACGAA AAAGCCT
Details for EncuA.01426.a.A1.GE27841
HARVESTED ON: 11/3/2009
SEQUENCED ON: 11/10/2009
EXPECTED MW: 37kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Validated AA Sequence
MGTLEAQTQG PGSMEKYENI KQVGEGAFGQ VYKSRRTSDG AVFAIKKMPV DRETGFPFTA IREIKLCKSV INKHIIGLDE IVFEEGFIFV VLEYMPYDLT GLLASGAKLS TDQIRSITSQ LIEAVSSMHG MGLVHRDIKP SNILLDCHGM LKLTDFGLTR EISGMMTNRV CTLWYRAPEL LLGETSYSLK VDAWSVGCIM LEMRLGRPPY RGSDEVSQIK LIFEELGIPQ DKYKWSDLLD VDIYSKSRST EEIIAERYGH LFDEEELKVL SGFLCLTSRK RLSVANSRYF TVISSHKNTY IPLSFEEAHE LYTKERKETK
Validated NT Sequence
tggctcatca ccatcaccat cntatgggta ccctggaagc tcagacccag ggtcctggtt cgatggagaa atacgaaaat atcaaacaag taggagaggg agcgttcggg caggtttaca agtccagaag aacatctgat ggggcagtgt ttgcaataaa aaagatgcca gtggacagag agaccggctt tccatttaca gccatacggg agataaagtt gtgcaagagc gtaattaata agcacataat agggcttgac gaaattgtgt tcgaggaggg cttcatattt gttgtacttg aatacatgcc atatgatctg acggggcttc ttgcatctgg cgccaagctc agcaccgacc agataagatc gatcacatcg caactcatag aggctgtgag ctccatgcac gggatggggc tggttcatcg agacataaag cccagcaata tcctgctaga ctgccacggg atgctgaagc tcacggactt tgggctgacc agagagatat cggggatgat gacgaacaga gtttgcacac tctggtacag ggcgcctgag ctattgcttg gagaaacatc ctacagcctt aaggtggatg cgtggtctgt tgggtgcata atgctagaaa tgaggcttgg gagaccgcca tatagaggaa gcgatgaagt atcgcagata aagctgatct tcgaggagct aggcatacct caggacaaat acaagtggtc tgacctgctt gacgttgata tttactccaa gagcaggagt accgaggaaa taattgccga gagatacgga catctgtttg acgaggaaga gctaaaggtt ctttctggat ttctttgcct aaccagtagg aagaggttgt ctgtggccaa ttctaggtat ttcaccgtca tctcaagcca caaaaacacc tatatcccac tgagctttga ggaagctcac gagctgtaca caaaggaaag gaaagaaacg aaaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEKYENIKQ VGEGAFGQVY KSRRTSDGAV FAIKKMPVDR ETGFPFTAIR EIKLCKSVIN KHIIGLDEIV FEEGFIFVVL EYMPYDLTGL LASGAKLSTD QIRSITSQLI EAVSSMHGMG LVHRDIKPSN ILLDCHGMLK LTDFGLTREI SGMMTNRVCT LWYRAPELLL GETSYSLKVD AWSVGCIMLE MRLGRPPYRG SDEVSQIKLI FEELGIPQDK YKWSDLLDVD IYSKSRSTEE IIAERYGHLF DEEELKVLSG FLCLTSRKRL SVANSRYFTV ISSHKNTYIP LSFEEAHELY TKERKETKKP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggagaaatac gaaaatatca aacaagtagg agagggagcg ttcgggcagg tttacaagtc cagaagaaca tctgatgggg cagtgtttgc aataaaaaag atgccagtgg acagagagac cggctttcca tttacagcca tacgggagat aaagttgtgc aagagcgtaa ttaataagca cataataggg cttgacgaaa ttgtgttcga ggagggcttc atatttgttg tacttgaata catgccatat gatctgacgg ggcttcttgc atctggcgcc aagctcagca ccgaccagat aagatcgatc acatcgcaac tcatagaggc tgtgagctcc atgcacggga tggggctggt tcatcgagac ataaagccca gcaatatcct gctagactgc cacgggatgc tgaagctcac ggactttggg ctgaccagag agatatcggg gatgatgacg aacagagttt gcacactctg gtacagggcg cctgagctat tgcttggaga aacatcctac agccttaagg tggatgcgtg gtctgttggg tgcataatgc tagaaatgag gcttgggaga ccgccatata gaggaagcga tgaagtatcg cagataaagc tgatcttcga ggagctaggc atacctcagg acaaatacaa gtggtctgac ctgcttgacg ttgatattta ctccaagagc aggagtaccg aggaaataat tgccgagaga tacggacatc tgtttgacga ggaagagcta aaggttcttt ctggatttct ttgcctaacc agtaggaaga ggttgtctgt ggccaattct aggtatttca ccgtcatctc aagccacaaa aacacctata tcccactgag ctttgaggaa gctcacgagc tgtacacaaa ggaaaggaaa gaaacgaaaa agcctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for EncuA.01426.a.A1.GE28354
HARVESTED ON: 1/5/2010
SEQUENCED ON: 1/15/2010
EXPECTED MW: 37kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Validated AA Sequence
MGTLEAQTQG PGSMEKYENI KQVGEGAFGQ VYKSRRTSDG AVFAIKKMPV DRETGFPFTA IREIKLCKSV INKHIIGLDE IVFEEGFIFV VLEYMPYDLT GLLASGAKLS TDQIRSITSQ LIEAVSSMHG MGLVHRDIKP SNILLDCHGM LKLTDFGLTR EISGMMTNRV CTLWYRAPEL LLGETSYSLK VDAWSVGCIM LEMRLGRPPY RGSDEVSQIK LIFEELGIPQ DKYKWSDLLD VDIYSKSRST EEIIAERYGH LFDEEELKVL SGFLCLTSRK RLSVANSRYF TVISSHKNTY IPLSFEEAHE LYTKERKETK
Validated NT Sequence
tggctcatca ccatcaccat cntatgggta ccctggaagc tcagacccag ggtcctggtt cgatggagaa atacgaaaat atcaaacaag taggagaggg agcgttcggg caggtttaca agtccagaag aacatctgat ggggcagtgt ttgcaataaa aaagatgcca gtggacagag agaccggctt tccatttaca gccatacggg agataaagtt gtgcaagagc gtaattaata agcacataat agggcttgac gaaattgtgt tcgaggaggg cttcatattt gttgtacttg aatacatgcc atatgatctg acggggcttc ttgcatctgg cgccaagctc agcaccgacc agataagatc gatcacatcg caactcatag aggctgtgag ctccatgcac gggatggggc tggttcatcg agacataaag cccagcaata tcctgctaga ctgccacggg atgctgaagc tcacggactt tgggctgacc agagagatat cggggatgat gacgaacaga gtttgcacac tctggtacag ggcgcctgag ctattgcttg gagaaacatc ctacagcctt aaggtggatg cgtggtctgt tgggtgcata atgctagaaa tgaggcttgg gagaccgcca tatagaggaa gcgatgaagt atcgcagata aagctgatct tcgaggagct aggcatacct caggacaaat acaagtggtc tgacctgctt gacgttgata tttactccaa gagcaggagt accgaggaaa taattgccga gagatacgga catctgtttg acgaggaaga gctaaaggtt ctttctggat ttctttgcct aaccagtagg aagaggttgt ctgtggccaa ttctaggtat ttcaccgtca tctcaagcca caaaaacacc tatatcccac tgagctttga ggaagctcac gagctgtaca caaaggaaag gaaagaaacg aaaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEKYENIKQ VGEGAFGQVY KSRRTSDGAV FAIKKMPVDR ETGFPFTAIR EIKLCKSVIN KHIIGLDEIV FEEGFIFVVL EYMPYDLTGL LASGAKLSTD QIRSITSQLI EAVSSMHGMG LVHRDIKPSN ILLDCHGMLK LTDFGLTREI SGMMTNRVCT LWYRAPELLL GETSYSLKVD AWSVGCIMLE MRLGRPPYRG SDEVSQIKLI FEELGIPQDK YKWSDLLDVD IYSKSRSTEE IIAERYGHLF DEEELKVLSG FLCLTSRKRL SVANSRYFTV ISSHKNTYIP LSFEEAHELY TKERKETKKP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggagaaatac gaaaatatca aacaagtagg agagggagcg ttcgggcagg tttacaagtc cagaagaaca tctgatgggg cagtgtttgc aataaaaaag atgccagtgg acagagagac cggctttcca tttacagcca tacgggagat aaagttgtgc aagagcgtaa ttaataagca cataataggg cttgacgaaa ttgtgttcga ggagggcttc atatttgttg tacttgaata catgccatat gatctgacgg ggcttcttgc atctggcgcc aagctcagca ccgaccagat aagatcgatc acatcgcaac tcatagaggc tgtgagctcc atgcacggga tggggctggt tcatcgagac ataaagccca gcaatatcct gctagactgc cacgggatgc tgaagctcac ggactttggg ctgaccagag agatatcggg gatgatgacg aacagagttt gcacactctg gtacagggcg cctgagctat tgcttggaga aacatcctac agccttaagg tggatgcgtg gtctgttggg tgcataatgc tagaaatgag gcttgggaga ccgccatata gaggaagcga tgaagtatcg cagataaagc tgatcttcga ggagctaggc atacctcagg acaaatacaa gtggtctgac ctgcttgacg ttgatattta ctccaagagc aggagtaccg aggaaataat tgccgagaga tacggacatc tgtttgacga ggaagagcta aaggttcttt ctggatttct ttgcctaacc agtaggaaga ggttgtctgt ggccaattct aggtatttca ccgtcatctc aagccacaaa aacacctata tcccactgag ctttgaggaa gctcacgagc tgtacacaaa ggaaaggaaa gaaacgaaaa agcctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for EncuA.01426.a.A1.GE29795
HARVESTED ON: 5/27/2010
SEQUENCED ON: 8/31/2010
EXPECTED MW: 37kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Validated AA Sequence
MGTLEAQTQG PGSMEKYENI KQVGEGAFGQ VYKSRRTSDG AVFAIKKMPV DRETGFPFTA IREIKLCKSV INKHIIGLDE IVFEEGFIFV VLEYMPYDLT GLLASGAKLS TDQIRSITSQ LIEAVSSMHG MGLVHRDIKP SNILLDCHGM LKLTDFGLTR EISGMMTNRV CTLWYRAPEL LLGETSYSLK VDAWSVGCIM LEMRLGRPPY RGSDEVSQIK LIFEELGIPQ DKYKWSDLLD VDIYSKSRST EEIIAERYGH LFDEEELKVL SGFLCLTSRK RLSVANSRYF TVISSHKNTY IPLSFEEAHE LYTKERKETK
Validated NT Sequence
tggctcatca ccatcaccat cntatgggta ccctggaagc tcagacccag ggtcctggtt cgatggagaa atacgaaaat atcaaacaag taggagaggg agcgttcggg caggtttaca agtccagaag aacatctgat ggggcagtgt ttgcaataaa aaagatgcca gtggacagag agaccggctt tccatttaca gccatacggg agataaagtt gtgcaagagc gtaattaata agcacataat agggcttgac gaaattgtgt tcgaggaggg cttcatattt gttgtacttg aatacatgcc atatgatctg acggggcttc ttgcatctgg cgccaagctc agcaccgacc agataagatc gatcacatcg caactcatag aggctgtgag ctccatgcac gggatggggc tggttcatcg agacataaag cccagcaata tcctgctaga ctgccacggg atgctgaagc tcacggactt tgggctgacc agagagatat cggggatgat gacgaacaga gtttgcacac tctggtacag ggcgcctgag ctattgcttg gagaaacatc ctacagcctt aaggtggatg cgtggtctgt tgggtgcata atgctagaaa tgaggcttgg gagaccgcca tatagaggaa gcgatgaagt atcgcagata aagctgatct tcgaggagct aggcatacct caggacaaat acaagtggtc tgacctgctt gacgttgata tttactccaa gagcaggagt accgaggaaa taattgccga gagatacgga catctgtttg acgaggaaga gctaaaggtt ctttctggat ttctttgcct aaccagtagg aagaggttgt ctgtggccaa ttctaggtat ttcaccgtca tctcaagcca caaaaacacc tatatcccac tgagctttga ggaagctcac gagctgtaca caaaggaaag gaaagaaacg aaaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEKYENIKQ VGEGAFGQVY KSRRTSDGAV FAIKKMPVDR ETGFPFTAIR EIKLCKSVIN KHIIGLDEIV FEEGFIFVVL EYMPYDLTGL LASGAKLSTD QIRSITSQLI EAVSSMHGMG LVHRDIKPSN ILLDCHGMLK LTDFGLTREI SGMMTNRVCT LWYRAPELLL GETSYSLKVD AWSVGCIMLE MRLGRPPYRG SDEVSQIKLI FEELGIPQDK YKWSDLLDVD IYSKSRSTEE IIAERYGHLF DEEELKVLSG FLCLTSRKRL SVANSRYFTV ISSHKNTYIP LSFEEAHELY TKERKETKKP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggagaaatac gaaaatatca aacaagtagg agagggagcg ttcgggcagg tttacaagtc cagaagaaca tctgatgggg cagtgtttgc aataaaaaag atgccagtgg acagagagac cggctttcca tttacagcca tacgggagat aaagttgtgc aagagcgtaa ttaataagca cataataggg cttgacgaaa ttgtgttcga ggagggcttc atatttgttg tacttgaata catgccatat gatctgacgg ggcttcttgc atctggcgcc aagctcagca ccgaccagat aagatcgatc acatcgcaac tcatagaggc tgtgagctcc atgcacggga tggggctggt tcatcgagac ataaagccca gcaatatcct gctagactgc cacgggatgc tgaagctcac ggactttggg ctgaccagag agatatcggg gatgatgacg aacagagttt gcacactctg gtacagggcg cctgagctat tgcttggaga aacatcctac agccttaagg tggatgcgtg gtctgttggg tgcataatgc tagaaatgag gcttgggaga ccgccatata gaggaagcga tgaagtatcg cagataaagc tgatcttcga ggagctaggc atacctcagg acaaatacaa gtggtctgac ctgcttgacg ttgatattta ctccaagagc aggagtaccg aggaaataat tgccgagaga tacggacatc tgtttgacga ggaagagcta aaggttcttt ctggatttct ttgcctaacc agtaggaaga ggttgtctgt ggccaattct aggtatttca ccgtcatctc aagccacaaa aacacctata tcccactgag ctttgaggaa gctcacgagc tgtacacaaa ggaaaggaaa gaaacgaaaa agcctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa