EncuA.01308.a

DNA REPAIR PROTEIN RAD51 HOMOLOG

CENTER ID: EncuA.01308.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.01308.a.A1.GE27839 Full length( EncuA.01308.a ) 1 334
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU11_0820
RefSeq: NP_586388.1
UniProt: Q8SQX0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSEEYQYQDT EYHSESISIS ELKNGGILAV DIAKLIEAGF TTVESLAFAP KRQLLSIKGF SDIKVDKLIK EAAKLVPMGF TTASAYHQRR SELVYLTTGS SEVDKLLSGG FESGSITEIF GEFRTGKTQL CHTVAVTCQL PPEQGGGGGK AMYIDTEGTF RSERLVPIAE RFGLDPNEVM DNISYARAYN SDHQSQLLIK ASAMMSESKY SVLIIDSATA LYRTDFSGRG ELGARQLHLA KYLRSLVNLA ETFRIAVIIT NQVVSNVDGA VGMFTGDIKK PIGGNIMAHA STTRLYLRKG RGETRICKIY DSPCLPESEA VFAITEQGIN DPDE
NT Sequence
ATGAGTGAGG AGTACCAGTA CCAGGACACG GAGTACCACT CCGAGAGCAT CTCGATATCC GAGCTGAAAA ATGGGGGAAT TCTGGCTGTG GACATAGCAA AGCTGATAGA AGCTGGGTTT ACAACGGTCG AATCGCTAGC ATTTGCTCCG AAGAGACAGC TTCTCTCGAT AAAGGGATTT TCCGACATCA AGGTGGATAA GCTCATCAAG GAAGCAGCAA AGCTAGTTCC GATGGGATTT ACAACGGCGT CTGCATACCA CCAAAGAAGG TCGGAGCTTG TCTACCTCAC TACTGGGTCT TCCGAGGTTG ACAAGCTGCT GAGCGGCGGG TTCGAGTCTG GGAGCATAAC AGAGATATTC GGGGAATTCC GCACCGGAAA GACACAGCTG TGCCATACGG TTGCAGTGAC GTGCCAGCTC CCTCCAGAGC AGGGTGGCGG AGGAGGAAAA GCCATGTACA TAGACACGGA GGGAACGTTC AGGTCTGAGA GGCTGGTTCC AATAGCAGAG CGATTTGGGC TGGATCCAAA CGAAGTCATG GACAATATAT CGTATGCCCG TGCCTACAAC TCCGACCACC AGTCCCAGCT CCTGATCAAG GCATCTGCAA TGATGTCTGA GTCAAAGTAC TCTGTCCTTA TAATTGACTC CGCAACTGCA CTCTACAGAA CAGACTTCAG TGGAAGGGGA GAGCTTGGAG CCAGGCAGCT GCATCTGGCA AAGTATCTCC GCAGCCTTGT CAATCTTGCC GAGACGTTCC GCATTGCTGT CATCATCACC AACCAGGTGG TTTCAAATGT AGACGGAGCA GTTGGCATGT TCACAGGAGA TATCAAGAAG CCAATAGGAG GAAACATCAT GGCACATGCA AGCACAACAA GGCTGTATCT GCGTAAGGGA AGGGGGGAGA CAAGGATATG CAAGATATAC GACTCTCCAT GCCTTCCCGA ATCCGAAGCT GTTTTTGCAA TCACTGAGCA GGGCATCAAT GATCCTGACG AA
Details for EncuA.01308.a.A1.GE27839
HARVESTED ON: 11/3/2009
SEQUENCED ON: 11/10/2009
EXPECTED MW: 38kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 96
Validated AA Sequence
MGTLEAQTQG PGSMSEEYQY QDTEYHSESI SISELKNGGI LAVDIAKLIE AGFTTVESLA FAPKRQLLSI KGFSDIKVDK LIKEAAKLVP MGFTTASAYH QRRSELVYLT TGSSEVDKLL SGGFESGSIT EIFGEFRTGK TQLCHTVAVT CQLPPEQGGG GGKAMYIDTE GTFRSERLVP IAERFGLDPN EVMDNISYAR AYNSDHQSQL LIKAPAMMSE SKYSVLIIDS ATALYRTDFS GRGELGARQL HLAKYLRSLV NLAETFRIAV IITNQVVSNV DGAVGMFTGD IKKPIGGNIM AHASTTRLYL RKGRGETRIC KIYDSPCLPE SEAVFAITEQ GIND
Validated NT Sequence
atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgagt gaggagtacc agtaccagga cacggagtac cactccgaga gcatctcgat atccgagctg aaaaatgggg gaattctggc tgtggacata gcaaagctga tagaagctgg gtttacaacg gtcgaatcgc tagcatttgc tccgaagaga cagcttctct cgataaaggg attttccgac atcaaggtgg ataagctcat caaggaagca gcaaagctag ttccgatggg atttacaacg gcgtctgcat accaccaaag aaggtcggag cttgtctacc tcactactgg gtcttccgag gttgacaagc tgctgagcgg cgggttcgag tctgggagca taacagagat attcggggaa ttccgcaccg gaaagacaca gctgtgccat acggttgcag tgacgtgcca gctccctcca gagcagggtg gcggaggagg aaaagccatg tacatagaca cggagggaac gttcaggtct gagaggctgg ttccaatagc agagcgattt gggctggatc caaacgaagt catggacaat atatcgtatg cccgtgccta caactccgac caccagtccc agctcctgat caaggcacct gcaatgatgt ctgagtcaaa gtactctgtc cttataattg actccgcaac tgcactctac agaacagact tcagtggaag gggagagctt ggagccaggc agctgcatct ggcaaagtat ctccgcagcc ttgtcaatct tgccgagacg ttccgcattg ctgtcatcat caccaaccag gtggtttcaa atgtagacgg agcagttggc atgttcacag gagatatcaa gaagccaata ggaggaaaca tcatggcaca tgcaagcaca acaaggctgt atctgcgtaa gggaaggggg gagacaagga tatgcaagat atacgactct ccatgccttc ccgaatccga agctgttttt gcaatcactg agcagggcat caatgatcc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSEEYQYQD TEYHSESISI SELKNGGILA VDIAKLIEAG FTTVESLAFA PKRQLLSIKG FSDIKVDKLI KEAAKLVPMG FTTASAYHQR RSELVYLTTG SSEVDKLLSG GFESGSITEI FGEFRTGKTQ LCHTVAVTCQ LPPEQGGGGG KAMYIDTEGT FRSERLVPIA ERFGLDPNEV MDNISYARAY NSDHQSQLLI KASAMMSESK YSVLIIDSAT ALYRTDFSGR GELGARQLHL AKYLRSLVNL AETFRIAVII TNQVVSNVDG AVGMFTGDIK KPIGGNIMAH ASTTRLYLRK GRGETRICKI YDSPCLPESE AVFAITEQGI NDPDE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtgaggag taccagtacc aggacacgga gtaccactcc gagagcatct cgatatccga gctgaaaaat gggggaattc tggctgtgga catagcaaag ctgatagaag ctgggtttac aacggtcgaa tcgctagcat ttgctccgaa gagacagctt ctctcgataa agggattttc cgacatcaag gtggataagc tcatcaagga agcagcaaag ctagttccga tgggatttac aacggcgtct gcataccacc aaagaaggtc ggagcttgtc tacctcacta ctgggtcttc cgaggttgac aagctgctga gcggcgggtt cgagtctggg agcataacag agatattcgg ggaattccgc accggaaaga cacagctgtg ccatacggtt gcagtgacgt gccagctccc tccagagcag ggtggcggag gaggaaaagc catgtacata gacacggagg gaacgttcag gtctgagagg ctggttccaa tagcagagcg atttgggctg gatccaaacg aagtcatgga caatatatcg tatgcccgtg cctacaactc cgaccaccag tcccagctcc tgatcaaggc atctgcaatg atgtctgagt caaagtactc tgtccttata attgactccg caactgcact ctacagaaca gacttcagtg gaaggggaga gcttggagcc aggcagctgc atctggcaaa gtatctccgc agccttgtca atcttgccga gacgttccgc attgctgtca tcatcaccaa ccaggtggtt tcaaatgtag acggagcagt tggcatgttc acaggagata tcaagaagcc aataggagga aacatcatgg cacatgcaag cacaacaagg ctgtatctgc gtaagggaag gggggagaca aggatatgca agatatacga ctctccatgc cttcccgaat ccgaagctgt ttttgcaatc actgagcagg gcatcaatga tcctgacgaa aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa