EncuA.01299.a

Putative leucine repeat-rich protein

CENTER ID: EncuA.01299.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.01299.a.A1.GE27836 Full length( EncuA.01299.a ) 1 218
EncuA.01299.a.A1.GE28349 Full length( EncuA.01299.a ) 1 218
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.01299.a.A1.PS00479 Full length( EncuA.01299.a ) 1 218
External Resources
RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU09_0460
OrthoMCL: OG5_127611
RefSeq: XP_955599.1
UniProt: Q8STV7
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDAVYIVHR QLEKIPTIPE HVRTVDLRRN NISRMTLNKA ESVEYLDLSD NRIRTISSLE NVPNLKVLDL SYNLITDISI PPMNLEELYL ISNDIATIHG LNLPRIKKLD MAVNDICKIE NLEKCTTLEE LYLGSNQIGA VEGLEEMRSL KILDLQNNKL ELVDCSMIPS SVEVLLLGEN RSLEVVENIE LLKNLKILGL EKTRVSENME GKSFTIWR
NT Sequence
ATGAGTGATG CTGTGTATAT AGTTCACAGA CAGCTGGAGA AGATACCGAC TATTCCAGAG CATGTGAGGA CTGTCGATCT GCGACGGAAC AACATATCTC GGATGACTCT AAACAAGGCG GAGTCTGTCG AGTACCTCGA TTTGTCGGAC AACAGAATAA GAACCATCTC TAGTCTTGAG AACGTTCCGA ACTTGAAGGT GCTGGATCTG AGCTACAACC TGATAACGGA CATCTCGATT CCTCCCATGA ATCTTGAGGA GCTGTACTTG ATTTCAAACG ACATTGCAAC GATCCACGGC CTAAACCTCC CTCGAATCAA GAAGTTAGAC ATGGCAGTAA ATGACATATG CAAGATCGAA AATCTCGAGA AATGCACCAC GCTGGAGGAG CTCTATCTCG GGAGCAACCA AATAGGGGCA GTGGAGGGCT TGGAGGAGAT GAGGAGCCTG AAGATACTGG ATCTCCAAAA CAACAAGCTC GAGCTTGTGG ACTGCTCGAT GATACCCAGT AGTGTTGAAG TTCTTCTTCT TGGAGAAAAC AGGAGTCTTG AAGTAGTGGA GAACATAGAG TTGCTCAAGA ACCTCAAAAT CCTTGGGCTG GAAAAGACAA GAGTGTCTGA GAATATGGAG GGGAAAAGCT TTACTATTTG GCGT
Details for EncuA.01299.a.A1.GE27836
HARVESTED ON: 11/3/2009
SEQUENCED ON: 11/10/2009
EXPECTED MW: 27kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSDAVYIVH RQLEKIPTIP EHVRTVDLRR NNISRMTLNK AESVEYLDLS DNRIRTISSL ENVPNLKVLD LSYNLITDIS IPPMNLEELY LISNDIATIH GLNLPRIKKL DMAVNDICKI ENLEKCTTLE ELYLGSNQIG AVEGLEEMRS LKILDLQNNK LELVDCSMIP SSVEVLLLGE NRSLEVVENI ELLKNLKILG LEKTRVSENM EGKSFTIWR
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagtg atgctgtgta tatagttcac agacagctgg agaagatacc gactattcca gagcatgtga ggactgtcga tctgcgacgg aacaacatat ctcggatgac tctaaacaag gcggagtctg tcgagtacct cgatttgtcg gacaacagaa taagaaccat ctctagtctt gagaacgttc cgaacttgaa ggtgctggat ctgagctaca acctgataac ggacatctcg attcctccca tgaatcttga ggagctgtac ttgatttcaa acgacattgc aacgatccac ggcctaaacc tccctcgaat caagaagtta gacatggcag taaatgacat atgcaagatc gaaaatctcg agaaatgcac cacgctggag gagctctatc tcgggagcaa ccaaataggg gcagtggagg gcttggagga gatgaggagc ctgaagatac tggatctcca aaacaacaag ctcgagcttg tggactgctc gatgataccc agtagtgttg aagttcttct tcttggagaa aacaggagtc ttgaagtagt ggagaacata gagttgctca agaacctcaa aatccttggg ctggaaaaga caagagtgtc tgagaatatg gaggggaaaa gctttactat ttggcgttaa taa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSDAVYIVH RQLEKIPTIP EHVRTVDLRR NNISRMTLNK AESVEYLDLS DNRIRTISSL ENVPNLKVLD LSYNLITDIS IPPMNLEELY LISNDIATIH GLNLPRIKKL DMAVNDICKI ENLEKCTTLE ELYLGSNQIG AVEGLEEMRS LKILDLQNNK LELVDCSMIP SSVEVLLLGE NRSLEVVENI ELLKNLKILG LEKTRVSENM EGKSFTIWR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtgatgct gtgtatatag ttcacagaca gctggagaag ataccgacta ttccagagca tgtgaggact gtcgatctgc gacggaacaa catatctcgg atgactctaa acaaggcgga gtctgtcgag tacctcgatt tgtcggacaa cagaataaga accatctcta gtcttgagaa cgttccgaac ttgaaggtgc tggatctgag ctacaacctg ataacggaca tctcgattcc tcccatgaat cttgaggagc tgtacttgat ttcaaacgac attgcaacga tccacggcct aaacctccct cgaatcaaga agttagacat ggcagtaaat gacatatgca agatcgaaaa tctcgagaaa tgcaccacgc tggaggagct ctatctcggg agcaaccaaa taggggcagt ggagggcttg gaggagatga ggagcctgaa gatactggat ctccaaaaca acaagctcga gcttgtggac tgctcgatga tacccagtag tgttgaagtt cttcttcttg gagaaaacag gagtcttgaa gtagtggaga acatagagtt gctcaagaac ctcaaaatcc ttgggctgga aaagacaaga gtgtctgaga atatggaggg gaaaagcttt actatttggc gtaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for EncuA.01299.a.A1.GE28349
HARVESTED ON: 1/5/2010
SEQUENCED ON: 1/15/2010
EXPECTED MW: 27kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSDAVYIVH RQLEKIPTIP EHVRTVDLRR NNISRMTLNK AESVEYLDLS DNRIRTISSL ENVPNLKVLD LSYNLITDIS IPPMNLEELY LISNDIATIH GLNLPRIKKL DMAVNDICKI ENLEKCTTLE ELYLGSNQIG AVEGLEEMRS LKILDLQNNK LELVDCSMIP SSVEVLLLGE NRSLEVVENI ELLKNLKILG LEKTRVSENM EGKSFTIWR
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagtg atgctgtgta tatagttcac agacagctgg agaagatacc gactattcca gagcatgtga ggactgtcga tctgcgacgg aacaacatat ctcggatgac tctaaacaag gcggagtctg tcgagtacct cgatttgtcg gacaacagaa taagaaccat ctctagtctt gagaacgttc cgaacttgaa ggtgctggat ctgagctaca acctgataac ggacatctcg attcctccca tgaatcttga ggagctgtac ttgatttcaa acgacattgc aacgatccac ggcctaaacc tccctcgaat caagaagtta gacatggcag taaatgacat atgcaagatc gaaaatctcg agaaatgcac cacgctggag gagctctatc tcgggagcaa ccaaataggg gcagtggagg gcttggagga gatgaggagc ctgaagatac tggatctcca aaacaacaag ctcgagcttg tggactgctc gatgataccc agtagtgttg aagttcttct tcttggagaa aacaggagtc ttgaagtagt ggagaacata gagttgctca agaacctcaa aatccttggg ctggaaaaga caagagtgtc tgagaatatg gaggggaaaa gctttactat ttggcgttaa taa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSDAVYIVH RQLEKIPTIP EHVRTVDLRR NNISRMTLNK AESVEYLDLS DNRIRTISSL ENVPNLKVLD LSYNLITDIS IPPMNLEELY LISNDIATIH GLNLPRIKKL DMAVNDICKI ENLEKCTTLE ELYLGSNQIG AVEGLEEMRS LKILDLQNNK LELVDCSMIP SSVEVLLLGE NRSLEVVENI ELLKNLKILG LEKTRVSENM EGKSFTIWR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtgatgct gtgtatatag ttcacagaca gctggagaag ataccgacta ttccagagca tgtgaggact gtcgatctgc gacggaacaa catatctcgg atgactctaa acaaggcgga gtctgtcgag tacctcgatt tgtcggacaa cagaataaga accatctcta gtcttgagaa cgttccgaac ttgaaggtgc tggatctgag ctacaacctg ataacggaca tctcgattcc tcccatgaat cttgaggagc tgtacttgat ttcaaacgac attgcaacga tccacggcct aaacctccct cgaatcaaga agttagacat ggcagtaaat gacatatgca agatcgaaaa tctcgagaaa tgcaccacgc tggaggagct ctatctcggg agcaaccaaa taggggcagt ggagggcttg gaggagatga ggagcctgaa gatactggat ctccaaaaca acaagctcga gcttgtggac tgctcgatga tacccagtag tgttgaagtt cttcttcttg gagaaaacag gagtcttgaa gtagtggaga acatagagtt gctcaagaac ctcaaaatcc ttgggctgga aaagacaaga gtgtctgaga atatggaggg gaaaagcttt actatttggc gtaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for EncuA.01299.a.A1.PS00479
PURIFICATION DATe: 2/4/2010
CONCENTRATION: 47.7mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSDAVYIVH RQLEKIPTIP EHVRTVDLRR NNISRMTLNK AESVEYLDLS DNRIRTISSL ENVPNLKVLD LSYNLITDIS IPPMNLEELY LISNDIATIH GLNLPRIKKL DMAVNDICKI ENLEKCTTLE ELYLGSNQIG AVEGLEEMRS LKILDLQNNK LELVDCSMIP SSVEVLLLGE NRSLEVVENI ELLKNLKILG LEKTRVSENM EGKSFTIWR
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagtg atgctgtgta tatagttcac agacagctgg agaagatacc gactattcca gagcatgtga ggactgtcga tctgcgacgg aacaacatat ctcggatgac tctaaacaag gcggagtctg tcgagtacct cgatttgtcg gacaacagaa taagaaccat ctctagtctt gagaacgttc cgaacttgaa ggtgctggat ctgagctaca acctgataac ggacatctcg attcctccca tgaatcttga ggagctgtac ttgatttcaa acgacattgc aacgatccac ggcctaaacc tccctcgaat caagaagtta gacatggcag taaatgacat atgcaagatc gaaaatctcg agaaatgcac cacgctggag gagctctatc tcgggagcaa ccaaataggg gcagtggagg gcttggagga gatgaggagc ctgaagatac tggatctcca aaacaacaag ctcgagcttg tggactgctc gatgataccc agtagtgttg aagttcttct tcttggagaa aacaggagtc ttgaagtagt ggagaacata gagttgctca agaacctcaa aatccttggg ctggaaaaga caagagtgtc tgagaatatg gaggggaaaa gctttactat ttggcgttaa taa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSDAVYIVH RQLEKIPTIP EHVRTVDLRR NNISRMTLNK AESVEYLDLS DNRIRTISSL ENVPNLKVLD LSYNLITDIS IPPMNLEELY LISNDIATIH GLNLPRIKKL DMAVNDICKI ENLEKCTTLE ELYLGSNQIG AVEGLEEMRS LKILDLQNNK LELVDCSMIP SSVEVLLLGE NRSLEVVENI ELLKNLKILG LEKTRVSENM EGKSFTIWR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGTGATGCT GTGTATATAG TTCACAGACA GCTGGAGAAG ATACCGACTA TTCCAGAGCA TGTGAGGACT GTCGATCTGC GACGGAACAA CATATCTCGG ATGACTCTAA ACAAGGCGGA GTCTGTCGAG TACCTCGATT TGTCGGACAA CAGAATAAGA ACCATCTCTA GTCTTGAGAA CGTTCCGAAC TTGAAGGTGC TGGATCTGAG CTACAACCTG ATAACGGACA TCTCGATTCC TCCCATGAAT CTTGAGGAGC TGTACTTGAT TTCAAACGAC ATTGCAACGA TCCACGGCCT AAACCTCCCT CGAATCAAGA AGTTAGACAT GGCAGTAAAT GACATATGCA AGATCGAAAA TCTCGAGAAA TGCACCACGC TGGAGGAGCT CTATCTCGGG AGCAACCAAA TAGGGGCAGT GGAGGGCTTG GAGGAGATGA GGAGCCTGAA GATACTGGAT CTCCAAAACA ACAAGCTCGA GCTTGTGGAC TGCTCGATGA TACCCAGTAG TGTTGAAGTT CTTCTTCTTG GAGAAAACAG GAGTCTTGAA GTAGTGGAGA ACATAGAGTT GCTCAAGAAC CTCAAAATCC TTGGGCTGGA AAAGACAAGA GTGTCTGAGA ATATGGAGGG GAAAAGCTTT ACTATTTGGC GTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA