EncuA.01191.a
Thymidylate synthase 1/2
| CENTER ID: | EncuA.01191.a |
| ORGANISM: | Encephalitozoon cuniculi GB-M1 |
| ASSOCIATED DISEASE: | Microsporidiosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I/IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| EncuA.01191.a.D11.GE00001 | EncuA.01191.a | 1 | 294 | ||
| EncuA.01191.a.D12.GE00001 | EncuA.01191.a | 1 | 260 | ||
| EncuA.01191.a.D14.GE00001 | EncuA.01191.a | 7 | 294 | ||
| EncuA.01191.a.D15.GE00001 | EncuA.01191.a | 7 | 260 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| EncuA.01191.a.D11.PD00045 | EncuA.01191.a | 1 | 294 | ||
| EncuA.01191.a.D11.PD00072 | EncuA.01191.a | 1 | 294 | ||
| EncuA.01191.a.D11.PD00132 | EncuA.01191.a | 1 | 294 |
Structures
3KGB
DEPOSITED: 10/28/2009
DETERMINATION: XRay
CLONE: EncuA.01191.a.D11.GE00001
PROTEIN: EncuA.01191.a.D11.PD00045
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | MicrosporidiaDB:ECU01_0180 |
| OrthoMCL: | OG5_127385 |
| RefSeq: | XP_965853.1 |
| UniProt: | O62584 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPQDPRHPEH QYLDLVKHIL ENGARRMDRT GTGTLSVFGA TMRFSLEDNT FPLLTTRRVF YRGVVEELLF FLRGETDSKV LEKKGVRIWE KNGAKQFLQS VGIDREEGDL GPIYGFQWRH FGARYETSAS SYEGKGVDQI ASAIAAIRAN PASRRIVVSA WNPTDLGSMA LPPCHVLFQF NVTDGKLSCA MYQRSGDMGL GVPFNIASYS LLTILVAHLT GLQPGEFVHF LGDAHVYLDH VDSLRQQIQR PPRAFPKLFV SPKGPRTEPE HFQYEDFELV GYDPHPAIKM NMSA
NT Sequence
atgccccagg acccgcgcca cccagagcac cagtatcttg acctggtgaa gcacatactg gaaaacggcg ccagaaggat ggacaggaca ggcacgggaa cgctgtccgt cttcggagca acaatgaggt tttccctcga agacaacacc ttcccgctgc tgacgacaag gagggtcttc tacagaggcg ttgtcgagga gctcctgttc ttcctgagag gcgagacaga ctcgaaggtc cttgagaaga aaggagtccg gatctgggaa aagaacgggg ccaagcagtt cctgcagtcc gtggggatag acagggagga gggcgacctc ggcccgatat acggcttcca gtggaggcac ttcggcgccc ggtacgagac atcggcctcg agctacgagg gcaagggggt cgaccagatc gcgagcgcca tcgcagcaat cagggccaac cccgccagca ggaggattgt tgtgagtgca tggaacccca cagacctcgg gagcatggcc cttcccccgt gccacgtgct cttccagttc aacgtgacgg acgggaagct cagctgtgca atgtaccaga ggtctggaga catgggcctg ggcgttccct tcaacattgc ctcgtactcg ctgctcacaa tactcgtggc gcatctgaca ggcctgcagc ccggggagtt tgttcacttt ctgggcgacg cccacgtgta tctggaccac gtcgactctc ttcggcagca gatccagaga cccccgagag catttccaaa gctctttgtc agcccaaagg ggccgagaac agagccggag cacttccagt acgaggactt cgagcttgtg ggatacgacc cgcaccctgc aatcaagatg aacatgagtg cttga
Details for EncuA.01191.a.D11.GE00001
| HARVESTED ON: | data unavailable |
| SEQUENCED ON: | 7/31/2009 |
| EXPECTED MW: | 0kDa |
| OBSERVED MW: | 0kDa |
| ANTIBIOTIC MARKER: | kanamycin |
| COUNT OF EXPRESSION COLONIES: | data unavailable |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 79 |
Validated AA Sequence
MXDNDIIEAX XXQIGXMPQD PRHPEHQYLD LVKHXLENGA RRMDRTGTGT LSVFGATMRF SXEDNTFPLL TTRRVFYRGV VEELLFFLRG ETDSKVLEKK GVRIWEKNGA KQFLQSVGID REEGDLGPIY GFQWRHFGAR YETSASSYEG KGVDQIASAI AAIRANPASR RIVVSAWNPT DLGSMALPPC HVLFQFNVTD GKLSCAMYQR SGDMGLGVPF NIASYSLLTI LVAHLTGLQP GEFVHFLGDA HVYLDHVDSL RQQIQRPPRA FPKLFVSPKG PRTEPEHFQY EDFELVGYDP HPAIKMNMSA
Validated NT Sequence
ttaagcagac atgttcatct tgatggctgg gtgcgggtca tagccaacta attcgaagtc ttcgtactgg aagtgttctg gttcggtacg cggacccttt ggcgatacga agagcttcgg gaatgcacgt ggagggcgct gaatctgttg gcgcagagaa tcaacgtggt ccagatatac atgggcgtca cccaggaagt ggacgaattc acctggctga aggccagtca gatgagcaac cagaatggtc agcagggagt aggaagcaat gttgaacggc acgcctaagc ccatgtcacc ggagcgttga tacatagcac aggacagttt gccatcggtc acgttgaact gaaacaggac gtggcacgga ggcagagcca tagagcctaa gtcagtcggg ttccaagcag acacaacgat acggcgagaa gcagggtttg cacggattgc tgcgattgca gaagctatct ggtccacgcc cttaccctcg taggaagatg cggaggtttc ataacgagcg ccgaagtgac gccactgaaa gccgtagatt ggaccgagat caccttcctc acgatcaatg cctacagact gcaggaactg tttagcaccg ttcttctccc agatacggac gccttttttc tccagaactt tagaatcagt ttcgccgcga aggaagaaga gcagttcttc aactacacca cggtagaaga cgcgacgggt ggtcagcagc gggaaggtgt tgtcttccan gctaaagcgc atggttgcgc cgaacacgct cagggtaccg gtgccggtac gatccatacg acgtgcgcca ttctcgagga natgcttaac taagtcgagg tactggtgtt ctgggtgacg cgggtcctgt ggcattncac cgatttgnnc anggngtgct tcgataatgt cgttatctnn cat
Expected Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMPQD PRHPEHQYLD LVKHILENGA RRMDRTGTGT LSVFGATMRF SLEDNTFPLL TTRRVFYRGV VEELLFFLRG ETDSKVLEKK GVRIWEKNGA KQFLQSVGID REEGDLGPIY GFQWRHFGAR YETSASSYEG KGVDQIASAI AAIRANPASR RIVVSAWNPT DLGSMALPPC HVLFQFNVTD GKLSCAMYQR SGDMGLGVPF NIASYSLLTI LVAHLTGLQP GEFVHFLGDA HVYLDHVDSL RQQIQRPPRA FPKLFVSPKG PRTEPEHFQY EDFELVGYDP HPAIKMNMSA
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggaat gccacaggac ccgcgtcacc cagaacacca gtacctcgac ttagttaagc atatcctcga gaatggcgca cgtcgtatgg atcgtaccgg caccggtacc ctgagcgtgt tcggcgcaac catgcgcttt agcctggaag acaacacctt cccgctgctg accacccgtc gcgtcttcta ccgtggtgta gttgaagaac tgctcttctt ccttcgcggc gaaactgatt ctaaagttct ggagaaaaaa ggcgtccgta tctgggagaa gaacggtgct aaacagttcc tgcagtctgt aggcattgat cgtgaggaag gtgatctcgg tccaatctac ggctttcagt ggcgtcactt cggcgctcgt tatgaaacct ccgcatcttc ctacgagggt aagggcgtgg accagatagc ttctgcaatc gcagcaatcc gtgcaaaccc tgcttctcgc cgtatcgttg tgtctgcttg gaacccgact gacttaggct ctatggctct gcctccgtgc cacgtcctgt ttcagttcaa cgtgaccgat ggcaaactgt cctgtgctat gtatcaacgc tccggtgaca tgggcttagg cgtgccgttc aacattgctt cctactccct gctgaccatt ctggttgctc atctgactgg ccttcagcca ggtgaattcg tccacttcct gggtgacgcc catgtatatc tggaccacgt tgattctctg cgccaacaga ttcagcgccc tccacgtgca ttcccgaagc tcttcgtatc gccaaagggt ccgcgtaccg aaccagaaca cttccagtac gaagacttcg aattagttgg ctatgacccg cacccagcca tcaagatgaa catgtctgct taaaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga t
Details for EncuA.01191.a.D12.GE00001
| HARVESTED ON: | data unavailable |
| SEQUENCED ON: | 7/31/2009 |
| EXPECTED MW: | 0kDa |
| OBSERVED MW: | 0kDa |
| ANTIBIOTIC MARKER: | kanamycin |
| COUNT OF EXPRESSION COLONIES: | data unavailable |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | No Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 87 |
Validated AA Sequence
MEAFAKRQGK EMDSLRFLYD GIRIQADQTP EDLDMEDNDI IEAHREQIGG MPQDPRHPEH QYLDLVKHIL ENGARRMDRT GTGTLSVFGA TMRFSLEDNT FPLLTTRRVF YRGVVEELLF FLRGETDSKV LEKKGVRIWE KNGAKQFLQS VGIDREEGDL GPIYGFQWRH FGARYETSAS SYEGKGVDQI ASAIAAIRAN PASRRIVVSA WNPTDLGSMA LPPCHVLFQF NVTDGKLSCA MYQRSGDMGL GVPFNIASYS LLTILVAHLT GLQPGEFVHF LGDAHVYLDH VDSLRQQIQR PPRAFPKLFV
Validated NT Sequence
ttatacgaag agcttcggga atgcacgtgg agggcgctga atctgttggc gcagagaatc aacgtggtcc agatatacat gggcgtcacc caggaagtgg acgaattcac ctggctgaag gccagtcaga tgagcaacca gaatggtcag cagggagtag gaagcaatgt tgaacggcac gcctaagccc atgtcaccgg agcgttgata catagcacag gacagtttgc catcggtcac gttgaactga aacaggacgt ggcacggagg cagagccata gagcctaagt cagtcgggtt ccaagcagac acaacgatac ggcgagaagc agggtttgca cggattgctg cgattgcaga agctatctgg tccacgccct taccctcgta ggaagatgcg gaggtttcat aacgagcgcc gaagtgacgc cactgaaagc cgtagattgg accgagatca ccttcctcac gatcaatgcc tacagactgc aggaactgtt tagcaccgtt cttctcccag atacggacgc cttttttctc cagaacttta gaatcagttt cgccgcgaag gaagaagagc agttcttcaa ctacaccacg gtagaagacg cgacgggtgg tcagcagcgg gaaggtgttg tcttccaggc taaagcgcat ggttgcgccg aacacgctca gggtaccggt gccggtacga tccatacgac gtgcgccatt ctcgaggata tgcttaacta agtcgaggta ctggtgttct gggtgacgcg ggtcctgtgg cattccaccg atttgttcac ggtgtgcttc gataatgtcg ttatcttcca tgtccagatc ttccggagtc tggtctgcct gaatacggat accgtcgtac agaaagcgca gggaatccat ttccttgccc tggcgtttag cgaatgcttc catcaggcga cgcagcgggg tggtcttctt aatcttgaag aagatttcgc tgctaccgtc gctaaccttc aggttgatgt gggtctccgg cttcacttcc ggttttactt cg
Expected Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMPQD PRHPEHQYLD LVKHILENGA RRMDRTGTGT LSVFGATMRF SLEDNTFPLL TTRRVFYRGV VEELLFFLRG ETDSKVLEKK GVRIWEKNGA KQFLQSVGID REEGDLGPIY GFQWRHFGAR YETSASSYEG KGVDQIASAI AAIRANPASR RIVVSAWNPT DLGSMALPPC HVLFQFNVTD GKLSCAMYQR SGDMGLGVPF NIASYSLLTI LVAHLTGLQP GEFVHFLGDA HVYLDHVDSL RQQIQRPPRA FPKLFV
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggaat gccacaggac ccgcgtcacc cagaacacca gtacctcgac ttagttaagc atatcctcga gaatggcgca cgtcgtatgg atcgtaccgg caccggtacc ctgagcgtgt tcggcgcaac catgcgcttt agcctggaag acaacacctt cccgctgctg accacccgtc gcgtcttcta ccgtggtgta gttgaagaac tgctcttctt ccttcgcggc gaaactgatt ctaaagttct ggagaaaaaa ggcgtccgta tctgggagaa gaacggtgct aaacagttcc tgcagtctgt aggcattgat cgtgaggaag gtgatctcgg tccaatctac ggctttcagt ggcgtcactt cggcgctcgt tatgaaacct ccgcatcttc ctacgagggt aagggcgtgg accagatagc ttctgcaatc gcagcaatcc gtgcaaaccc tgcttctcgc cgtatcgttg tgtctgcttg gaacccgact gacttaggct ctatggctct gcctccgtgc cacgtcctgt ttcagttcaa cgtgaccgat ggcaaactgt cctgtgctat gtatcaacgc tccggtgaca tgggcttagg cgtgccgttc aacattgctt cctactccct gctgaccatt ctggttgctc atctgactgg ccttcagcca ggtgaattcg tccacttcct gggtgacgcc catgtatatc tggaccacgt tgattctctg cgccaacaga ttcagcgccc tccacgtgca ttcccgaagc tcttcgtata aaagcttgcg gccgcactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggat
Details for EncuA.01191.a.D14.GE00001
| HARVESTED ON: | data unavailable |
| SEQUENCED ON: | 7/31/2009 |
| EXPECTED MW: | 0kDa |
| OBSERVED MW: | 0kDa |
| ANTIBIOTIC MARKER: | kanamycin |
| COUNT OF EXPRESSION COLONIES: | data unavailable |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGHPEH QYLDLVKHIL ENGARRMDRT GTGTLSVFGA TMRFSLEDNT FPLLTTRRVF YRGVVEELLF FLRGETDSKV LEKKGVRIWE KNGAKQFLQS VGIDREEGDL GPIYGFQWRH FGARYETSAS SYEGKGVDQI ASAIAAIRAN PASRRIVVSA WNPTDLGSMA LPPCHVLFQF NVTDGKLSCA MYQRSGDMGL GVPFNIASYS LLTILVAHLT GLQPGEFVHF LGDAHVYLDH VDSLRQQIQR PPRAFPKLFV SPKGPRTEPE HFQYEDFELV GYDPHPAIKM NMSA
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggaca cccagaacac cagtacctcg acttagttaa gcatatcctc gagaatggcg cacgtcgtat ggatcgtacc ggcaccggta ccctgagcgt gttcggcgca accatgcgct ttagcctgga agacaacacc ttcccgctgc tgaccacccg tcgcgtcttc taccgtggtg tagttgaaga actgctcttc ttccttcgcg gcgaaactga ttctaaagtt ctggagaaaa aaggcgtccg tatctgggag aagaacggtg ctaaacagtt cctgcagtct gtaggcattg atcgtgagga aggtgatctc ggtccaatct acggctttca gtggcgtcac ttcggcgctc gttatgaaac ctccgcatct tcctacgagg gtaagggcgt ggaccagata gcttctgcaa tcgcagcaat ccgtgcaaac cctgcttctc gccgtatcgt tgtgtctgct tggaacccga ctgacttagg ctctatggct ctgcctccgt gccacgtcct gtttcagttc aacgtgaccg atggcaaact gtcctgtgct atgtatcaac gctccggtga catgggctta ggcgtgccgt tcaacattgc ttcctactcc ctgctgacca ttctggttgc tcatctgact ggccttcagc caggtgaatt cgtccacttc ctgggtgacg cccatgtata tctggaccac gttgattctc tgcgccaaca gattcagcgc cctccacgtg cattcccgaa gctcttcgta tcgccaaagg gtccgcgtac cgaaccagaa cacttccagt acgaagactt cgaattagtt ggctatgacc cgcacccagc catcaagatg aacatgtctg cttaaaagct tgcggccgca ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gat
Details for EncuA.01191.a.D15.GE00001
| HARVESTED ON: | data unavailable |
| SEQUENCED ON: | 7/31/2009 |
| EXPECTED MW: | 0kDa |
| OBSERVED MW: | 0kDa |
| ANTIBIOTIC MARKER: | kanamycin |
| COUNT OF EXPRESSION COLONIES: | data unavailable |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | No Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 18 |
| PERCENT COVERAGE: | 21 |
Validated AA Sequence
MHVEGAESVG AXNXRGPXIH GRHXXXGRXH LAEGQSDXXX ERSXXXRKXX XXXXXXXXXG XLXXXGCLLS XXLN
Validated NT Sequence
tnnnnggnnn nntnncagcc ggatctcagn ggnggnggng gnggngntcg nnngcggccg cnagctttta tacnaagagc ttcgngaatg cacgtggagg gcgctgaatc tgttggcgca nanaatcnac gtggtccana tatacatggn cgtcacncan gaannggacg anttcacctg gctgaaggcc agtcagatga ncanncagaa cgntcnncan ggantaggaa gcangnnnna nggnncgnct annnnnatgn cnccgganng ttgannnnan taggatgttt gttatcgnnc annttgaact gaaannnaat ngncncnana nncntncagc cnaagnnatc nggtncctnn nnnnagnnnn ngcnngannc gnntnccnna tannnnnngn nnnntnntng nnnannnntn ntncnntann nnnnncnnnn nnnnnnnttn nnnnncncnn nnnnnnnnna taannnnnnn cnnnnnnnnn ngnnnnnnnt nnnnnntnnn nnnnnnannn ntnncnnatn nanntnccnt nnnnnantnn gnnannnt
Expected Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGHPEH QYLDLVKHIL ENGARRMDRT GTGTLSVFGA TMRFSLEDNT FPLLTTRRVF YRGVVEELLF FLRGETDSKV LEKKGVRIWE KNGAKQFLQS VGIDREEGDL GPIYGFQWRH FGARYETSAS SYEGKGVDQI ASAIAAIRAN PASRRIVVSA WNPTDLGSMA LPPCHVLFQF NVTDGKLSCA MYQRSGDMGL GVPFNIASYS LLTILVAHLT GLQPGEFVHF LGDAHVYLDH VDSLRQQIQR PPRAFPKLFV
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggaca cccagaacac cagtacctcg acttagttaa gcatatcctc gagaatggcg cacgtcgtat ggatcgtacc ggcaccggta ccctgagcgt gttcggcgca accatgcgct ttagcctgga agacaacacc ttcccgctgc tgaccacccg tcgcgtcttc taccgtggtg tagttgaaga actgctcttc ttccttcgcg gcgaaactga ttctaaagtt ctggagaaaa aaggcgtccg tatctgggag aagaacggtg ctaaacagtt cctgcagtct gtaggcattg atcgtgagga aggtgatctc ggtccaatct acggctttca gtggcgtcac ttcggcgctc gttatgaaac ctccgcatct tcctacgagg gtaagggcgt ggaccagata gcttctgcaa tcgcagcaat ccgtgcaaac cctgcttctc gccgtatcgt tgtgtctgct tggaacccga ctgacttagg ctctatggct ctgcctccgt gccacgtcct gtttcagttc aacgtgaccg atggcaaact gtcctgtgct atgtatcaac gctccggtga catgggctta ggcgtgccgt tcaacattgc ttcctactcc ctgctgacca ttctggttgc tcatctgact ggccttcagc caggtgaatt cgtccacttc ctgggtgacg cccatgtata tctggaccac gttgattctc tgcgccaaca gattcagcgc cctccacgtg cattcccgaa gctcttcgta taaaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga t
Details for EncuA.01191.a.D11.PD00045
| PURIFICATION DATe: | 3/26/2009 |
| CONCENTRATION: | 11mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 300µl |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 79 |
Protocol Notes
notes unavailable
Validated AA Sequence
MXDNDIIEAX XXQIGXMPQD PRHPEHQYLD LVKHXLENGA RRMDRTGTGT LSVFGATMRF SXEDNTFPLL TTRRVFYRGV VEELLFFLRG ETDSKVLEKK GVRIWEKNGA KQFLQSVGID REEGDLGPIY GFQWRHFGAR YETSASSYEG KGVDQIASAI AAIRANPASR RIVVSAWNPT DLGSMALPPC HVLFQFNVTD GKLSCAMYQR SGDMGLGVPF NIASYSLLTI LVAHLTGLQP GEFVHFLGDA HVYLDHVDSL RQQIQRPPRA FPKLFVSPKG PRTEPEHFQY EDFELVGYDP HPAIKMNMSA
Validated NT Sequence
ttaagcagac atgttcatct tgatggctgg gtgcgggtca tagccaacta attcgaagtc ttcgtactgg aagtgttctg gttcggtacg cggacccttt ggcgatacga agagcttcgg gaatgcacgt ggagggcgct gaatctgttg gcgcagagaa tcaacgtggt ccagatatac atgggcgtca cccaggaagt ggacgaattc acctggctga aggccagtca gatgagcaac cagaatggtc agcagggagt aggaagcaat gttgaacggc acgcctaagc ccatgtcacc ggagcgttga tacatagcac aggacagttt gccatcggtc acgttgaact gaaacaggac gtggcacgga ggcagagcca tagagcctaa gtcagtcggg ttccaagcag acacaacgat acggcgagaa gcagggtttg cacggattgc tgcgattgca gaagctatct ggtccacgcc cttaccctcg taggaagatg cggaggtttc ataacgagcg ccgaagtgac gccactgaaa gccgtagatt ggaccgagat caccttcctc acgatcaatg cctacagact gcaggaactg tttagcaccg ttcttctccc agatacggac gccttttttc tccagaactt tagaatcagt ttcgccgcga aggaagaaga gcagttcttc aactacacca cggtagaaga cgcgacgggt ggtcagcagc gggaaggtgt tgtcttccan gctaaagcgc atggttgcgc cgaacacgct cagggtaccg gtgccggtac gatccatacg acgtgcgcca ttctcgagga natgcttaac taagtcgagg tactggtgtt ctgggtgacg cgggtcctgt ggcattncac cgatttgnnc anggngtgct tcgataatgt cgttatctnn cat
Expressed Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMPQD PRHPEHQYLD LVKHILENGA RRMDRTGTGT LSVFGATMRF SLEDNTFPLL TTRRVFYRGV VEELLFFLRG ETDSKVLEKK GVRIWEKNGA KQFLQSVGID REEGDLGPIY GFQWRHFGAR YETSASSYEG KGVDQIASAI AAIRANPASR RIVVSAWNPT DLGSMALPPC HVLFQFNVTD GKLSCAMYQR SGDMGLGVPF NIASYSLLTI LVAHLTGLQP GEFVHFLGDA HVYLDHVDSL RQQIQRPPRA FPKLFVSPKG PRTEPEHFQY EDFELVGYDP HPAIKMNMSA
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCCACC ACCACCACCA CCATAGCGGC GAAGTAAAAC CGGAAGTGAA GCCGGAGACC CACATCAACC TGAAGGTTAG CGACGGTAGC AGCGAAATCT TCTTCAAGAT TAAGAAGACC ACCCCGCTGC GTCGCCTGAT GGAAGCATTC GCTAAACGCC AGGGCAAGGA AATGGATTCC CTGCGCTTTC TGTACGACGG TATCCGTATT CAGGCAGACC AGACTCCGGA AGATCTGGAC ATGGAAGATA ACGACATTAT CGAAGCACAC CGTGAACAAA TCGGTGGAAT GCCACAGGAC CCGCGTCACC CAGAACACCA GTACCTCGAC TTAGTTAAGC ATATCCTCGA GAATGGCGCA CGTCGTATGG ATCGTACCGG CACCGGTACC CTGAGCGTGT TCGGCGCAAC CATGCGCTTT AGCCTGGAAG ACAACACCTT CCCGCTGCTG ACCACCCGTC GCGTCTTCTA CCGTGGTGTA GTTGAAGAAC TGCTCTTCTT CCTTCGCGGC GAAACTGATT CTAAAGTTCT GGAGAAAAAA GGCGTCCGTA TCTGGGAGAA GAACGGTGCT AAACAGTTCC TGCAGTCTGT AGGCATTGAT CGTGAGGAAG GTGATCTCGG TCCAATCTAC GGCTTTCAGT GGCGTCACTT CGGCGCTCGT TATGAAACCT CCGCATCTTC CTACGAGGGT AAGGGCGTGG ACCAGATAGC TTCTGCAATC GCAGCAATCC GTGCAAACCC TGCTTCTCGC CGTATCGTTG TGTCTGCTTG GAACCCGACT GACTTAGGCT CTATGGCTCT GCCTCCGTGC CACGTCCTGT TTCAGTTCAA CGTGACCGAT GGCAAACTGT CCTGTGCTAT GTATCAACGC TCCGGTGACA TGGGCTTAGG CGTGCCGTTC AACATTGCTT CCTACTCCCT GCTGACCATT CTGGTTGCTC ATCTGACTGG CCTTCAGCCA GGTGAATTCG TCCACTTCCT GGGTGACGCC CATGTATATC TGGACCACGT TGATTCTCTG CGCCAACAGA TTCAGCGCCC TCCACGTGCA TTCCCGAAGC TCTTCGTATC GCCAAAGGGT CCGCGTACCG AACCAGAACA CTTCCAGTAC GAAGACTTCG AATTAGTTGG CTATGACCCG CACCCAGCCA TCAAGATGAA CATGTCTGCT TAAAAGCTTG CGGCCGCACT CGAGCACCAC CACCACCACC ACTGAGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA T
Details for EncuA.01191.a.D11.PD00072
| PURIFICATION DATe: | 7/7/2009 |
| CONCENTRATION: | 26.19mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | data unavailable |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 79 |
Protocol Notes
notes unavailable
Validated AA Sequence
MXDNDIIEAX XXQIGXMPQD PRHPEHQYLD LVKHXLENGA RRMDRTGTGT LSVFGATMRF SXEDNTFPLL TTRRVFYRGV VEELLFFLRG ETDSKVLEKK GVRIWEKNGA KQFLQSVGID REEGDLGPIY GFQWRHFGAR YETSASSYEG KGVDQIASAI AAIRANPASR RIVVSAWNPT DLGSMALPPC HVLFQFNVTD GKLSCAMYQR SGDMGLGVPF NIASYSLLTI LVAHLTGLQP GEFVHFLGDA HVYLDHVDSL RQQIQRPPRA FPKLFVSPKG PRTEPEHFQY EDFELVGYDP HPAIKMNMSA
Validated NT Sequence
ttaagcagac atgttcatct tgatggctgg gtgcgggtca tagccaacta attcgaagtc ttcgtactgg aagtgttctg gttcggtacg cggacccttt ggcgatacga agagcttcgg gaatgcacgt ggagggcgct gaatctgttg gcgcagagaa tcaacgtggt ccagatatac atgggcgtca cccaggaagt ggacgaattc acctggctga aggccagtca gatgagcaac cagaatggtc agcagggagt aggaagcaat gttgaacggc acgcctaagc ccatgtcacc ggagcgttga tacatagcac aggacagttt gccatcggtc acgttgaact gaaacaggac gtggcacgga ggcagagcca tagagcctaa gtcagtcggg ttccaagcag acacaacgat acggcgagaa gcagggtttg cacggattgc tgcgattgca gaagctatct ggtccacgcc cttaccctcg taggaagatg cggaggtttc ataacgagcg ccgaagtgac gccactgaaa gccgtagatt ggaccgagat caccttcctc acgatcaatg cctacagact gcaggaactg tttagcaccg ttcttctccc agatacggac gccttttttc tccagaactt tagaatcagt ttcgccgcga aggaagaaga gcagttcttc aactacacca cggtagaaga cgcgacgggt ggtcagcagc gggaaggtgt tgtcttccan gctaaagcgc atggttgcgc cgaacacgct cagggtaccg gtgccggtac gatccatacg acgtgcgcca ttctcgagga natgcttaac taagtcgagg tactggtgtt ctgggtgacg cgggtcctgt ggcattncac cgatttgnnc anggngtgct tcgataatgt cgttatctnn cat
Expressed Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMPQD PRHPEHQYLD LVKHILENGA RRMDRTGTGT LSVFGATMRF SLEDNTFPLL TTRRVFYRGV VEELLFFLRG ETDSKVLEKK GVRIWEKNGA KQFLQSVGID REEGDLGPIY GFQWRHFGAR YETSASSYEG KGVDQIASAI AAIRANPASR RIVVSAWNPT DLGSMALPPC HVLFQFNVTD GKLSCAMYQR SGDMGLGVPF NIASYSLLTI LVAHLTGLQP GEFVHFLGDA HVYLDHVDSL RQQIQRPPRA FPKLFVSPKG PRTEPEHFQY EDFELVGYDP HPAIKMNMSA
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCCACC ACCACCACCA CCATAGCGGC GAAGTAAAAC CGGAAGTGAA GCCGGAGACC CACATCAACC TGAAGGTTAG CGACGGTAGC AGCGAAATCT TCTTCAAGAT TAAGAAGACC ACCCCGCTGC GTCGCCTGAT GGAAGCATTC GCTAAACGCC AGGGCAAGGA AATGGATTCC CTGCGCTTTC TGTACGACGG TATCCGTATT CAGGCAGACC AGACTCCGGA AGATCTGGAC ATGGAAGATA ACGACATTAT CGAAGCACAC CGTGAACAAA TCGGTGGAAT GCCACAGGAC CCGCGTCACC CAGAACACCA GTACCTCGAC TTAGTTAAGC ATATCCTCGA GAATGGCGCA CGTCGTATGG ATCGTACCGG CACCGGTACC CTGAGCGTGT TCGGCGCAAC CATGCGCTTT AGCCTGGAAG ACAACACCTT CCCGCTGCTG ACCACCCGTC GCGTCTTCTA CCGTGGTGTA GTTGAAGAAC TGCTCTTCTT CCTTCGCGGC GAAACTGATT CTAAAGTTCT GGAGAAAAAA GGCGTCCGTA TCTGGGAGAA GAACGGTGCT AAACAGTTCC TGCAGTCTGT AGGCATTGAT CGTGAGGAAG GTGATCTCGG TCCAATCTAC GGCTTTCAGT GGCGTCACTT CGGCGCTCGT TATGAAACCT CCGCATCTTC CTACGAGGGT AAGGGCGTGG ACCAGATAGC TTCTGCAATC GCAGCAATCC GTGCAAACCC TGCTTCTCGC CGTATCGTTG TGTCTGCTTG GAACCCGACT GACTTAGGCT CTATGGCTCT GCCTCCGTGC CACGTCCTGT TTCAGTTCAA CGTGACCGAT GGCAAACTGT CCTGTGCTAT GTATCAACGC TCCGGTGACA TGGGCTTAGG CGTGCCGTTC AACATTGCTT CCTACTCCCT GCTGACCATT CTGGTTGCTC ATCTGACTGG CCTTCAGCCA GGTGAATTCG TCCACTTCCT GGGTGACGCC CATGTATATC TGGACCACGT TGATTCTCTG CGCCAACAGA TTCAGCGCCC TCCACGTGCA TTCCCGAAGC TCTTCGTATC GCCAAAGGGT CCGCGTACCG AACCAGAACA CTTCCAGTAC GAAGACTTCG AATTAGTTGG CTATGACCCG CACCCAGCCA TCAAGATGAA CATGTCTGCT TAAAAGCTTG CGGCCGCACT CGAGCACCAC CACCACCACC ACTGAGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA T
Details for EncuA.01191.a.D11.PD00132
| PURIFICATION DATe: | 5/14/2010 |
| CONCENTRATION: | 0.7mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | data unavailable |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 79 |
Protocol Notes
notes unavailable
Validated AA Sequence
MXDNDIIEAX XXQIGXMPQD PRHPEHQYLD LVKHXLENGA RRMDRTGTGT LSVFGATMRF SXEDNTFPLL TTRRVFYRGV VEELLFFLRG ETDSKVLEKK GVRIWEKNGA KQFLQSVGID REEGDLGPIY GFQWRHFGAR YETSASSYEG KGVDQIASAI AAIRANPASR RIVVSAWNPT DLGSMALPPC HVLFQFNVTD GKLSCAMYQR SGDMGLGVPF NIASYSLLTI LVAHLTGLQP GEFVHFLGDA HVYLDHVDSL RQQIQRPPRA FPKLFVSPKG PRTEPEHFQY EDFELVGYDP HPAIKMNMSA
Validated NT Sequence
ttaagcagac atgttcatct tgatggctgg gtgcgggtca tagccaacta attcgaagtc ttcgtactgg aagtgttctg gttcggtacg cggacccttt ggcgatacga agagcttcgg gaatgcacgt ggagggcgct gaatctgttg gcgcagagaa tcaacgtggt ccagatatac atgggcgtca cccaggaagt ggacgaattc acctggctga aggccagtca gatgagcaac cagaatggtc agcagggagt aggaagcaat gttgaacggc acgcctaagc ccatgtcacc ggagcgttga tacatagcac aggacagttt gccatcggtc acgttgaact gaaacaggac gtggcacgga ggcagagcca tagagcctaa gtcagtcggg ttccaagcag acacaacgat acggcgagaa gcagggtttg cacggattgc tgcgattgca gaagctatct ggtccacgcc cttaccctcg taggaagatg cggaggtttc ataacgagcg ccgaagtgac gccactgaaa gccgtagatt ggaccgagat caccttcctc acgatcaatg cctacagact gcaggaactg tttagcaccg ttcttctccc agatacggac gccttttttc tccagaactt tagaatcagt ttcgccgcga aggaagaaga gcagttcttc aactacacca cggtagaaga cgcgacgggt ggtcagcagc gggaaggtgt tgtcttccan gctaaagcgc atggttgcgc cgaacacgct cagggtaccg gtgccggtac gatccatacg acgtgcgcca ttctcgagga natgcttaac taagtcgagg tactggtgtt ctgggtgacg cgggtcctgt ggcattncac cgatttgnnc anggngtgct tcgataatgt cgttatctnn cat
Expressed Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMPQD PRHPEHQYLD LVKHILENGA RRMDRTGTGT LSVFGATMRF SLEDNTFPLL TTRRVFYRGV VEELLFFLRG ETDSKVLEKK GVRIWEKNGA KQFLQSVGID REEGDLGPIY GFQWRHFGAR YETSASSYEG KGVDQIASAI AAIRANPASR RIVVSAWNPT DLGSMALPPC HVLFQFNVTD GKLSCAMYQR SGDMGLGVPF NIASYSLLTI LVAHLTGLQP GEFVHFLGDA HVYLDHVDSL RQQIQRPPRA FPKLFVSPKG PRTEPEHFQY EDFELVGYDP HPAIKMNMSA
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCCACC ACCACCACCA CCATAGCGGC GAAGTAAAAC CGGAAGTGAA GCCGGAGACC CACATCAACC TGAAGGTTAG CGACGGTAGC AGCGAAATCT TCTTCAAGAT TAAGAAGACC ACCCCGCTGC GTCGCCTGAT GGAAGCATTC GCTAAACGCC AGGGCAAGGA AATGGATTCC CTGCGCTTTC TGTACGACGG TATCCGTATT CAGGCAGACC AGACTCCGGA AGATCTGGAC ATGGAAGATA ACGACATTAT CGAAGCACAC CGTGAACAAA TCGGTGGAAT GCCACAGGAC CCGCGTCACC CAGAACACCA GTACCTCGAC TTAGTTAAGC ATATCCTCGA GAATGGCGCA CGTCGTATGG ATCGTACCGG CACCGGTACC CTGAGCGTGT TCGGCGCAAC CATGCGCTTT AGCCTGGAAG ACAACACCTT CCCGCTGCTG ACCACCCGTC GCGTCTTCTA CCGTGGTGTA GTTGAAGAAC TGCTCTTCTT CCTTCGCGGC GAAACTGATT CTAAAGTTCT GGAGAAAAAA GGCGTCCGTA TCTGGGAGAA GAACGGTGCT AAACAGTTCC TGCAGTCTGT AGGCATTGAT CGTGAGGAAG GTGATCTCGG TCCAATCTAC GGCTTTCAGT GGCGTCACTT CGGCGCTCGT TATGAAACCT CCGCATCTTC CTACGAGGGT AAGGGCGTGG ACCAGATAGC TTCTGCAATC GCAGCAATCC GTGCAAACCC TGCTTCTCGC CGTATCGTTG TGTCTGCTTG GAACCCGACT GACTTAGGCT CTATGGCTCT GCCTCCGTGC CACGTCCTGT TTCAGTTCAA CGTGACCGAT GGCAAACTGT CCTGTGCTAT GTATCAACGC TCCGGTGACA TGGGCTTAGG CGTGCCGTTC AACATTGCTT CCTACTCCCT GCTGACCATT CTGGTTGCTC ATCTGACTGG CCTTCAGCCA GGTGAATTCG TCCACTTCCT GGGTGACGCC CATGTATATC TGGACCACGT TGATTCTCTG CGCCAACAGA TTCAGCGCCC TCCACGTGCA TTCCCGAAGC TCTTCGTATC GCCAAAGGGT CCGCGTACCG AACCAGAACA CTTCCAGTAC GAAGACTTCG AATTAGTTGG CTATGACCCG CACCCAGCCA TCAAGATGAA CATGTCTGCT TAAAAGCTTG CGGCCGCACT CGAGCACCAC CACCACCACC ACTGAGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA T