EncuA.01171.a

ATP-dependent rRNA helicase RRP3

CENTER ID: EncuA.01171.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.01171.a.A1.GE27833 Full length( EncuA.01171.a ) 1 400
EncuA.01171.a.A1.GE28345 Full length( EncuA.01171.a ) 1 400
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.01171.a.A1.PW27724 Full length( EncuA.01171.a ) 1 400
External Resources
RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU10_0440
OrthoMCL: OG5_127784
RefSeq: NP_586159.1
UniProt: Q8SR63
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEFGDLRIDE SLIKTCQEKG ITRPTEVQRQ VIPAVLGGGD VIAVSQTGSG KTLAFVLPIV SHLLQKNRSF YCLVVAPTRE LSSQIAECFN MFQATGLRVC LLVGGANFNV QANQLSKRPH VVVGTPGRIA EHVLKTKSFR TERVRKFVLD EADRFFEQDF VEDLETIIPS LREKRQTLLF TATMSDEISK LSSSILKRPK TIRTAEKYET VPALKEYYLF VAMKWKNSAL VELLEMSQGM SVIVFVSMCV TARVMSLALA RLGFCSEALH GELSQEKREE AMRSFKESRF NVLVCTDLGS RGLDISHVDL VINFDVPKSG KDYIHRVGRT ARAGRSGTAI TLVTQYDVEQ IQKIEFTLEK KLEEFKMMKK NFGTICARIE EAIQEAQETL KEERKRNRRP
NT Sequence
ATGGAATTTG GTGATCTAAG AATTGATGAG TCCTTGATAA AGACATGCCA GGAAAAGGGG ATTACACGGC CTACTGAGGT CCAAAGACAG GTGATTCCAG CAGTGCTTGG AGGAGGTGAT GTTATAGCGG TTTCGCAGAC TGGGTCAGGA AAAACCCTTG CGTTTGTCCT TCCGATAGTT TCCCATCTGT TGCAGAAAAA CAGATCATTC TATTGCCTAG TCGTTGCACC CACAAGAGAG CTATCCTCAC AAATAGCTGA GTGCTTTAAC ATGTTCCAGG CCACGGGGCT GAGGGTCTGT CTGCTGGTCG GAGGAGCCAA CTTCAATGTT CAGGCCAACC AGCTTTCTAA GCGTCCCCAC GTGGTTGTTG GAACGCCCGG AAGAATAGCG GAACATGTCT TGAAAACAAA ATCGTTCAGA ACAGAAAGAG TTCGGAAGTT TGTTCTCGAT GAAGCGGACA GGTTTTTTGA GCAAGACTTT GTAGAAGATC TGGAGACTAT TATTCCGAGT CTGAGGGAGA AAAGGCAGAC TCTTCTCTTT ACGGCAACGA TGTCCGACGA AATATCTAAG CTGTCCAGTT CCATCTTAAA AAGACCGAAA ACCATCCGCA CGGCAGAGAA GTATGAGACG GTTCCTGCCC TGAAAGAGTA CTACCTCTTT GTGGCCATGA AATGGAAGAA CTCTGCATTG GTTGAATTGC TGGAAATGAG CCAAGGAATG TCTGTTATTG TGTTTGTATC GATGTGTGTG ACTGCCAGAG TAATGTCCTT AGCATTGGCC AGACTTGGGT TCTGCTCGGA GGCGCTACAT GGGGAGCTGA GCCAGGAAAA AAGAGAAGAG GCCATGAGAA GTTTTAAGGA AAGCAGGTTT AATGTTCTTG TTTGCACTGA CCTTGGAAGT AGAGGTCTTG ATATAAGCCA TGTAGATCTG GTGATTAATT TTGATGTCCC GAAGAGCGGA AAGGATTATA TCCATCGGGT CGGGAGGACT GCAAGGGCTG GAAGATCTGG AACTGCTATC ACGCTTGTAA CCCAGTACGA TGTTGAACAG ATCCAAAAGA TAGAATTTAC ACTAGAAAAA AAGCTGGAGG AATTCAAGAT GATGAAGAAG AACTTCGGGA CAATTTGTGC AAGAATCGAA GAAGCCATCC AAGAAGCTCA AGAGACTCTT AAGGAGGAGA GAAAAAGAAA CAGAAGGCCA
Details for EncuA.01171.a.A1.GE27833
HARVESTED ON: 11/3/2009
SEQUENCED ON: 12/10/2009
EXPECTED MW: 47kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMEFGDLRID ESLIKTCQEK GITRPTEVQR QVIPAVLGGG DVIAVSQTGS GKTLAFVLPI VSHLLQKNRS FYCLVVAPTR ELSSQIAECF NMFQATGLRV CLLVGGANFN VQANQLSKRP HVVVGTPGRI AEHVLKTKSF RTERVRKFVL DEADRFFEQD FVEDLETIIP SLREKRQTLL FTATMSDEIX KLSSSILKRP KTIRTAEKYE TVPALKEYYL FVAMKWKNSA LVELLEMSQG MSVIVFVSMC VTARVMSLAL ARLGFCSEAL HGELSQEKRE EAMRSFKESR FNVLVCTDLG SRGLDISHVD LVINFDVPKS GKDYIHRVGR TARAGRSGTA ITLVTQYDVE QIQKIEFTLE KKLEEFKMMK KNFGTICARI EEAIQEAQET LKEERKRNRR P
Validated NT Sequence
taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg gaatttggtg atctaagaat tgatgagtcc ttgataaaga catgccagga aaaggggatt acacggccta ctgaggtcca aagacaggtg attccagcag tgcttggagg aggtgatgtt atagcggttt cgcagactgg gtcaggaaaa acccttgcgt ttgtccttcc gatagtttcc catctgttgc agaaaaacag atcattctat tgcctagtcg ttgcacccac aagagagcta tcctcacaaa tagctgagtg ctttaacatg ttccaggcca cggggctgag ggtctgtctg ctggtcggag gagccaactt caatgttcag gccaaccagc tttctaagcg tccccacgtg gttgttggaa cgcccggaag aatagcggaa catgtcttga aaacaaaatc gttcagaaca gaaagagttc ggaagtttgt tctcgatgaa gcggacaggt tttttgagca agactttgta gaagatctgg agactattat tccgagtctg agggagaaaa ggcagactct tctctttacg gcaacgatgt ccgacgaaat atntaagctg tccagttcca tcttaaaaag accgaaaacc atccgcacgg cagagaagta tgagacggtt cctgccctga aagagtacta cctctttgtg gccatgaaat ggaagaactc tgcattggtt gaattgctgg aaatgagcca aggaatgtct gttattgtgt ttgtatcgat gtgtgtgact gccagagtaa tgtccttagc attggccaga cttgggttct gctcggaggc gctacatggg gagctgagcc aggaaaaaag agaagaggcc atgagaagtt ttaaggaaag caggtttaat gttcttgttt gcactgacct tggaagtaga ggtcttgata taagccatgt agatctggtg attaattttg atgtcccgaa gagcggaaag gattatatcc atcgggtcgg gaggactgca agggctggaa gatctggaac tgctatcacg cttgtaaccc agtacgatgt tgaacagatc caaaagatag aatttacact agaaaaaaag ctggaggaat tcaagatgat gaagaagaac ttcgggacaa tttgtgcaag aatcgaagaa gccatccaag aagctcaaga gactcttaag gaggagagaa aaagaaacag aaggccataa taa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEFGDLRID ESLIKTCQEK GITRPTEVQR QVIPAVLGGG DVIAVSQTGS GKTLAFVLPI VSHLLQKNRS FYCLVVAPTR ELSSQIAECF NMFQATGLRV CLLVGGANFN VQANQLSKRP HVVVGTPGRI AEHVLKTKSF RTERVRKFVL DEADRFFEQD FVEDLETIIP SLREKRQTLL FTATMSDEIS KLSSSILKRP KTIRTAEKYE TVPALKEYYL FVAMKWKNSA LVELLEMSQG MSVIVFVSMC VTARVMSLAL ARLGFCSEAL HGELSQEKRE EAMRSFKESR FNVLVCTDLG SRGLDISHVD LVINFDVPKS GKDYIHRVGR TARAGRSGTA ITLVTQYDVE QIQKIEFTLE KKLEEFKMMK KNFGTICARI EEAIQEAQET LKEERKRNRR P
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaatttggt gatctaagaa ttgatgagtc cttgataaag acatgccagg aaaaggggat tacacggcct actgaggtcc aaagacaggt gattccagca gtgcttggag gaggtgatgt tatagcggtt tcgcagactg ggtcaggaaa aacccttgcg tttgtccttc cgatagtttc ccatctgttg cagaaaaaca gatcattcta ttgcctagtc gttgcaccca caagagagct atcctcacaa atagctgagt gctttaacat gttccaggcc acggggctga gggtctgtct gctggtcgga ggagccaact tcaatgttca ggccaaccag ctttctaagc gtccccacgt ggttgttgga acgcccggaa gaatagcgga acatgtcttg aaaacaaaat cgttcagaac agaaagagtt cggaagtttg ttctcgatga agcggacagg ttttttgagc aagactttgt agaagatctg gagactatta ttccgagtct gagggagaaa aggcagactc ttctctttac ggcaacgatg tccgacgaaa tatctaagct gtccagttcc atcttaaaaa gaccgaaaac catccgcacg gcagagaagt atgagacggt tcctgccctg aaagagtact acctctttgt ggccatgaaa tggaagaact ctgcattggt tgaattgctg gaaatgagcc aaggaatgtc tgttattgtg tttgtatcga tgtgtgtgac tgccagagta atgtccttag cattggccag acttgggttc tgctcggagg cgctacatgg ggagctgagc caggaaaaaa gagaagaggc catgagaagt tttaaggaaa gcaggtttaa tgttcttgtt tgcactgacc ttggaagtag aggtcttgat ataagccatg tagatctggt gattaatttt gatgtcccga agagcggaaa ggattatatc catcgggtcg ggaggactgc aagggctgga agatctggaa ctgctatcac gcttgtaacc cagtacgatg ttgaacagat ccaaaagata gaatttacac tagaaaaaaa gctggaggaa ttcaagatga tgaagaagaa cttcgggaca atttgtgcaa gaatcgaaga agccatccaa gaagctcaag agactcttaa ggaggagaga aaaagaaaca gaaggccaaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for EncuA.01171.a.A1.GE28345
HARVESTED ON: 1/5/2010
SEQUENCED ON: 1/15/2010
EXPECTED MW: 47kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMEFGDLRID ESLIKTCQEK GITRPTEVQR QVIPAVLGGG DVIAVSQTGS GKTLAFVLPI VSHLLQKNRS FYCLVVAPTR ELSSQIAECF NMFQATGLRV CLLVGGANFN VQANQLSKRP HVVVGTPGRI AEHVLKTKSF RTERVRKFVL DEADRFFEQD FVEDLETIIP SLREKRQTLL FTATMSDEIX KLSSSILKRP KTIRTAEKYE TVPALKEYYL FVAMKWKNSA LVELLEMSQG MSVIVFVSMC VTARVMSLAL ARLGFCSEAL HGELSQEKRE EAMRSFKESR FNVLVCTDLG SRGLDISHVD LVINFDVPKS GKDYIHRVGR TARAGRSGTA ITLVTQYDVE QIQKIEFTLE KKLEEFKMMK KNFGTICARI EEAIQEAQET LKEERKRNRR P
Validated NT Sequence
taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg gaatttggtg atctaagaat tgatgagtcc ttgataaaga catgccagga aaaggggatt acacggccta ctgaggtcca aagacaggtg attccagcag tgcttggagg aggtgatgtt atagcggttt cgcagactgg gtcaggaaaa acccttgcgt ttgtccttcc gatagtttcc catctgttgc agaaaaacag atcattctat tgcctagtcg ttgcacccac aagagagcta tcctcacaaa tagctgagtg ctttaacatg ttccaggcca cggggctgag ggtctgtctg ctggtcggag gagccaactt caatgttcag gccaaccagc tttctaagcg tccccacgtg gttgttggaa cgcccggaag aatagcggaa catgtcttga aaacaaaatc gttcagaaca gaaagagttc ggaagtttgt tctcgatgaa gcggacaggt tttttgagca agactttgta gaagatctgg agactattat tccgagtctg agggagaaaa ggcagactct tctctttacg gcaacgatgt ccgacgaaat atntaagctg tccagttcca tcttaaaaag accgaaaacc atccgcacgg cagagaagta tgagacggtt cctgccctga aagagtacta cctctttgtg gccatgaaat ggaagaactc tgcattggtt gaattgctgg aaatgagcca aggaatgtct gttattgtgt ttgtatcgat gtgtgtgact gccagagtaa tgtccttagc attggccaga cttgggttct gctcggaggc gctacatggg gagctgagcc aggaaaaaag agaagaggcc atgagaagtt ttaaggaaag caggtttaat gttcttgttt gcactgacct tggaagtaga ggtcttgata taagccatgt agatctggtg attaattttg atgtcccgaa gagcggaaag gattatatcc atcgggtcgg gaggactgca agggctggaa gatctggaac tgctatcacg cttgtaaccc agtacgatgt tgaacagatc caaaagatag aatttacact agaaaaaaag ctggaggaat tcaagatgat gaagaagaac ttcgggacaa tttgtgcaag aatcgaagaa gccatccaag aagctcaaga gactcttaag gaggagagaa aaagaaacag aaggccataa taa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEFGDLRID ESLIKTCQEK GITRPTEVQR QVIPAVLGGG DVIAVSQTGS GKTLAFVLPI VSHLLQKNRS FYCLVVAPTR ELSSQIAECF NMFQATGLRV CLLVGGANFN VQANQLSKRP HVVVGTPGRI AEHVLKTKSF RTERVRKFVL DEADRFFEQD FVEDLETIIP SLREKRQTLL FTATMSDEIS KLSSSILKRP KTIRTAEKYE TVPALKEYYL FVAMKWKNSA LVELLEMSQG MSVIVFVSMC VTARVMSLAL ARLGFCSEAL HGELSQEKRE EAMRSFKESR FNVLVCTDLG SRGLDISHVD LVINFDVPKS GKDYIHRVGR TARAGRSGTA ITLVTQYDVE QIQKIEFTLE KKLEEFKMMK KNFGTICARI EEAIQEAQET LKEERKRNRR P
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaatttggt gatctaagaa ttgatgagtc cttgataaag acatgccagg aaaaggggat tacacggcct actgaggtcc aaagacaggt gattccagca gtgcttggag gaggtgatgt tatagcggtt tcgcagactg ggtcaggaaa aacccttgcg tttgtccttc cgatagtttc ccatctgttg cagaaaaaca gatcattcta ttgcctagtc gttgcaccca caagagagct atcctcacaa atagctgagt gctttaacat gttccaggcc acggggctga gggtctgtct gctggtcgga ggagccaact tcaatgttca ggccaaccag ctttctaagc gtccccacgt ggttgttgga acgcccggaa gaatagcgga acatgtcttg aaaacaaaat cgttcagaac agaaagagtt cggaagtttg ttctcgatga agcggacagg ttttttgagc aagactttgt agaagatctg gagactatta ttccgagtct gagggagaaa aggcagactc ttctctttac ggcaacgatg tccgacgaaa tatctaagct gtccagttcc atcttaaaaa gaccgaaaac catccgcacg gcagagaagt atgagacggt tcctgccctg aaagagtact acctctttgt ggccatgaaa tggaagaact ctgcattggt tgaattgctg gaaatgagcc aaggaatgtc tgttattgtg tttgtatcga tgtgtgtgac tgccagagta atgtccttag cattggccag acttgggttc tgctcggagg cgctacatgg ggagctgagc caggaaaaaa gagaagaggc catgagaagt tttaaggaaa gcaggtttaa tgttcttgtt tgcactgacc ttggaagtag aggtcttgat ataagccatg tagatctggt gattaatttt gatgtcccga agagcggaaa ggattatatc catcgggtcg ggaggactgc aagggctgga agatctggaa ctgctatcac gcttgtaacc cagtacgatg ttgaacagat ccaaaagata gaatttacac tagaaaaaaa gctggaggaa ttcaagatga tgaagaagaa cttcgggaca atttgtgcaa gaatcgaaga agccatccaa gaagctcaag agactcttaa ggaggagaga aaaagaaaca gaaggccaaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for EncuA.01171.a.A1.PW27724
PURIFICATION DATe: 3/15/2010
CONCENTRATION: 43.6mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 17
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMEFGDLRID ESLIKTCQEK GITRPTEVQR QVIPAVLGGG DVIAVSQTGS GKTLAFVLPI VSHLLQKNRS FYCLVVAPTR ELSSQIAECF NMFQATGLRV CLLVGGANFN VQANQLSKRP HVVVGTPGRI AEHVLKTKSF RTERVRKFVL DEADRFFEQD FVEDLETIIP SLREKRQTLL FTATMSDEIX KLSSSILKRP KTIRTAEKYE TVPALKEYYL FVAMKWKNSA LVELLEMSQG MSVIVFVSMC VTARVMSLAL ARLGFCSEAL HGELSQEKRE EAMRSFKESR FNVLVCTDLG SRGLDISHVD LVINFDVPKS GKDYIHRVGR TARAGRSGTA ITLVTQYDVE QIQKIEFTLE KKLEEFKMMK KNFGTICARI EEAIQEAQET LKEERKRNRR P
Validated NT Sequence
taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg gaatttggtg atctaagaat tgatgagtcc ttgataaaga catgccagga aaaggggatt acacggccta ctgaggtcca aagacaggtg attccagcag tgcttggagg aggtgatgtt atagcggttt cgcagactgg gtcaggaaaa acccttgcgt ttgtccttcc gatagtttcc catctgttgc agaaaaacag atcattctat tgcctagtcg ttgcacccac aagagagcta tcctcacaaa tagctgagtg ctttaacatg ttccaggcca cggggctgag ggtctgtctg ctggtcggag gagccaactt caatgttcag gccaaccagc tttctaagcg tccccacgtg gttgttggaa cgcccggaag aatagcggaa catgtcttga aaacaaaatc gttcagaaca gaaagagttc ggaagtttgt tctcgatgaa gcggacaggt tttttgagca agactttgta gaagatctgg agactattat tccgagtctg agggagaaaa ggcagactct tctctttacg gcaacgatgt ccgacgaaat atntaagctg tccagttcca tcttaaaaag accgaaaacc atccgcacgg cagagaagta tgagacggtt cctgccctga aagagtacta cctctttgtg gccatgaaat ggaagaactc tgcattggtt gaattgctgg aaatgagcca aggaatgtct gttattgtgt ttgtatcgat gtgtgtgact gccagagtaa tgtccttagc attggccaga cttgggttct gctcggaggc gctacatggg gagctgagcc aggaaaaaag agaagaggcc atgagaagtt ttaaggaaag caggtttaat gttcttgttt gcactgacct tggaagtaga ggtcttgata taagccatgt agatctggtg attaattttg atgtcccgaa gagcggaaag gattatatcc atcgggtcgg gaggactgca agggctggaa gatctggaac tgctatcacg cttgtaaccc agtacgatgt tgaacagatc caaaagatag aatttacact agaaaaaaag ctggaggaat tcaagatgat gaagaagaac ttcgggacaa tttgtgcaag aatcgaagaa gccatccaag aagctcaaga gactcttaag gaggagagaa aaagaaacag aaggccataa taa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEFGDLRID ESLIKTCQEK GITRPTEVQR QVIPAVLGGG DVIAVSQTGS GKTLAFVLPI VSHLLQKNRS FYCLVVAPTR ELSSQIAECF NMFQATGLRV CLLVGGANFN VQANQLSKRP HVVVGTPGRI AEHVLKTKSF RTERVRKFVL DEADRFFEQD FVEDLETIIP SLREKRQTLL FTATMSDEIS KLSSSILKRP KTIRTAEKYE TVPALKEYYL FVAMKWKNSA LVELLEMSQG MSVIVFVSMC VTARVMSLAL ARLGFCSEAL HGELSQEKRE EAMRSFKESR FNVLVCTDLG SRGLDISHVD LVINFDVPKS GKDYIHRVGR TARAGRSGTA ITLVTQYDVE QIQKIEFTLE KKLEEFKMMK KNFGTICARI EEAIQEAQET LKEERKRNRR P
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGAATTTGGT GATCTAAGAA TTGATGAGTC CTTGATAAAG ACATGCCAGG AAAAGGGGAT TACACGGCCT ACTGAGGTCC AAAGACAGGT GATTCCAGCA GTGCTTGGAG GAGGTGATGT TATAGCGGTT TCGCAGACTG GGTCAGGAAA AACCCTTGCG TTTGTCCTTC CGATAGTTTC CCATCTGTTG CAGAAAAACA GATCATTCTA TTGCCTAGTC GTTGCACCCA CAAGAGAGCT ATCCTCACAA ATAGCTGAGT GCTTTAACAT GTTCCAGGCC ACGGGGCTGA GGGTCTGTCT GCTGGTCGGA GGAGCCAACT TCAATGTTCA GGCCAACCAG CTTTCTAAGC GTCCCCACGT GGTTGTTGGA ACGCCCGGAA GAATAGCGGA ACATGTCTTG AAAACAAAAT CGTTCAGAAC AGAAAGAGTT CGGAAGTTTG TTCTCGATGA AGCGGACAGG TTTTTTGAGC AAGACTTTGT AGAAGATCTG GAGACTATTA TTCCGAGTCT GAGGGAGAAA AGGCAGACTC TTCTCTTTAC GGCAACGATG TCCGACGAAA TATCTAAGCT GTCCAGTTCC ATCTTAAAAA GACCGAAAAC CATCCGCACG GCAGAGAAGT ATGAGACGGT TCCTGCCCTG AAAGAGTACT ACCTCTTTGT GGCCATGAAA TGGAAGAACT CTGCATTGGT TGAATTGCTG GAAATGAGCC AAGGAATGTC TGTTATTGTG TTTGTATCGA TGTGTGTGAC TGCCAGAGTA ATGTCCTTAG CATTGGCCAG ACTTGGGTTC TGCTCGGAGG CGCTACATGG GGAGCTGAGC CAGGAAAAAA GAGAAGAGGC CATGAGAAGT TTTAAGGAAA GCAGGTTTAA TGTTCTTGTT TGCACTGACC TTGGAAGTAG AGGTCTTGAT ATAAGCCATG TAGATCTGGT GATTAATTTT GATGTCCCGA AGAGCGGAAA GGATTATATC CATCGGGTCG GGAGGACTGC AAGGGCTGGA AGATCTGGAA CTGCTATCAC GCTTGTAACC CAGTACGATG TTGAACAGAT CCAAAAGATA GAATTTACAC TAGAAAAAAA GCTGGAGGAA TTCAAGATGA TGAAGAAGAA CTTCGGGACA ATTTGTGCAA GAATCGAAGA AGCCATCCAA GAAGCTCAAG AGACTCTTAA GGAGGAGAGA AAAAGAAACA GAAGGCCAAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA