EncuA.01167.a

REPLICATION FACTOR C

CENTER ID: EncuA.01167.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.01167.a.A1.GE27831 Full length( EncuA.01167.a ) 1 309
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU09_1330
RefSeq: XP_955685.1
UniProt: Q8SQM0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDLLVNKYQP SEIQDIVGNE ATMELVSLMI ESRDMPHLLF TGPPGTGKTT CAKILARRLL GNKEGLLELN ASDERGIDTV RTTIKSFAQR RVKDCEFKII ILDEADSMTT TAQQAMRRVM EIHSSECRFI LICNVFTKIF EPIQSRCAIL RFDRIEQSVI LKRLKEISEG EGIRITAEAL DLVVELSDGD MRQSLNILQA CINSPGTVDQ DYIIKIIGLP SPKRIEKVLQ RLLKREVEEA LEMFDEIWEE KFDPLDLINS FFRAAKNMES YELLKVIGLA NLRISEGVNS RLQFYGMFWD ILDMGSKRL
NT Sequence
ATGGATCTGC TTGTGAACAA GTACCAGCCA AGCGAGATCC AGGACATAGT CGGAAACGAA GCAACGATGG AACTCGTTTC CTTGATGATC GAAAGCAGAG ATATGCCCCA CCTGCTGTTC ACGGGGCCTC CCGGGACAGG AAAGACAACC TGTGCAAAAA TACTTGCAAG GAGGCTCCTG GGCAACAAGG AGGGGCTGCT GGAGCTGAAT GCGTCTGACG AAAGAGGGAT AGACACTGTT AGGACCACCA TTAAAAGCTT TGCACAGAGA AGAGTCAAGG ACTGTGAGTT CAAGATCATA ATCCTGGACG AGGCCGACTC GATGACCACT ACAGCCCAGC AGGCAATGCG AAGGGTGATG GAGATACACA GCTCGGAGTG CAGGTTTATT CTGATCTGTA ATGTGTTTAC AAAGATATTT GAGCCTATCC AGAGCAGATG TGCAATACTA AGGTTTGACA GGATAGAGCA GTCCGTGATC CTCAAAAGAC TAAAGGAAAT CAGCGAAGGC GAAGGCATCA GGATAACCGC CGAGGCTCTT GATCTTGTTG TCGAGCTTAG CGATGGGGAC ATGAGACAGT CGCTGAACAT CCTTCAAGCG TGCATCAACT CTCCTGGCAC AGTAGACCAG GACTATATCA TCAAGATAAT AGGCCTTCCA TCTCCAAAGC GGATCGAAAA GGTTCTCCAG AGGCTCCTGA AAAGGGAAGT AGAGGAGGCC CTGGAGATGT TTGACGAGAT CTGGGAGGAG AAATTTGATC CTCTCGACCT CATAAACTCG TTTTTCAGGG CTGCAAAGAA CATGGAAAGC TACGAGTTGC TTAAGGTGAT TGGGCTGGCA AACCTAAGGA TATCTGAGGG TGTCAACTCA AGGCTGCAGT TCTATGGAAT GTTCTGGGAC ATCCTCGACA TGGGAAGTAA AAGATTG
Details for EncuA.01167.a.A1.GE27831
HARVESTED ON: 11/3/2009
SEQUENCED ON: 11/10/2009
EXPECTED MW: 37kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAXTQG PGSMDLLVNK YQPSEIQDIV GNXATMELVS LMIESRDMPH LLFXGPPGTG KTTCAKILAR RLLGNKEGLL ELNASDERGI DTVRTXXXSF AQRXVKDCEF KIIILDEADS MTTTAQQAMR RVXEIHSSEC RFILICNVFT KIXEPIQSRC AILRFDRIEQ SVILKRLKEI SEGEGIRITA EALDLVVELS DGDMRQSLNI LQACINSPGT VDQDYIIKII GLPSPKRIEK VLQRLLKREV EEALEMFDEI WEEKFDPLDL INSFFRAAKN MESYELLKVI GLANLRISEG VNSRLQFYGM FWDILDMGSK RL
Validated NT Sequence
acaatctttt acttcccatg tcgaggatgt cccagaacat tccatagaac tgcagccttg agttgacacc ctcagatatc cttaggtttg ccagcccaat caccttaagc aactcgtagc tttccatgtt ctttgcagcc ctgaaaaacg agtttatgag gtcgagagga tcaaatttct cctcccagat ctcgtcaaac atctccaggg cctcctctac ttcccttttc aggagcctct ggagaacctt ttcgatccgc tttggagatg gaaggcctat tatcttgatg atatagtcct ggtctactgt gccaggagag ttgatgcacg cttgaaggat gttcagcgac tgtctcatgt ccccatcgct aagctcgaca acaagatcaa gagcctcggc ggttatcctg atgccttcgc cttcgctgat ttcctttagt cttttgagga tcacggactg ctctatcctg tcaaacctta gtattgcaca tctgctctgg ataggctcan atatctttgt aaacacatta cagatcagaa taaacctgca ctccgagctg tgtatctccn ncacccttcg cattgcctgc tgggctgtag tggtcatcga gtcngcctcg tccaggatta tgatcttgaa ctcacagtcc ttgactcntc tctgtgcaaa gctnnnnnnn nnggtcctaa cagtgtctat ccctctttcg tcagacgcat tcagctccag cagcccctcc ttgttgccca ggagcctcct tgcaagtatt tttgcacagg ttgtctttcc tgtcccggga ggccccnnga acagcaggtg gggcatatct ctgctttcga tcatcaagga aacgagttcc atcgttgctt ngtttccgac tatgtcctgg atctcgcttg gctggtactt gttcacaagc agatccatcg aaccaggacc ctgggtnnga gcttccaggg tacccatnnn a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDLLVNKYQ PSEIQDIVGN EATMELVSLM IESRDMPHLL FTGPPGTGKT TCAKILARRL LGNKEGLLEL NASDERGIDT VRTTIKSFAQ RRVKDCEFKI IILDEADSMT TTAQQAMRRV MEIHSSECRF ILICNVFTKI FEPIQSRCAI LRFDRIEQSV ILKRLKEISE GEGIRITAEA LDLVVELSDG DMRQSLNILQ ACINSPGTVD QDYIIKIIGL PSPKRIEKVL QRLLKREVEE ALEMFDEIWE EKFDPLDLIN SFFRAAKNME SYELLKVIGL ANLRISEGVN SRLQFYGMFW DILDMGSKRL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatctgctt gtgaacaagt accagccaag cgagatccag gacatagtcg gaaacgaagc aacgatggaa ctcgtttcct tgatgatcga aagcagagat atgccccacc tgctgttcac ggggcctccc gggacaggaa agacaacctg tgcaaaaata cttgcaagga ggctcctggg caacaaggag gggctgctgg agctgaatgc gtctgacgaa agagggatag acactgttag gaccaccatt aaaagctttg cacagagaag agtcaaggac tgtgagttca agatcataat cctggacgag gccgactcga tgaccactac agcccagcag gcaatgcgaa gggtgatgga gatacacagc tcggagtgca ggtttattct gatctgtaat gtgtttacaa agatatttga gcctatccag agcagatgtg caatactaag gtttgacagg atagagcagt ccgtgatcct caaaagacta aaggaaatca gcgaaggcga aggcatcagg ataaccgccg aggctcttga tcttgttgtc gagcttagcg atggggacat gagacagtcg ctgaacatcc ttcaagcgtg catcaactct cctggcacag tagaccagga ctatatcatc aagataatag gccttccatc tccaaagcgg atcgaaaagg ttctccagag gctcctgaaa agggaagtag aggaggccct ggagatgttt gacgagatct gggaggagaa atttgatcct ctcgacctca taaactcgtt tttcagggct gcaaagaaca tggaaagcta cgagttgctt aaggtgattg ggctggcaaa cctaaggata tctgagggtg tcaactcaag gctgcagttc tatggaatgt tctgggacat cctcgacatg ggaagtaaaa gattgaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa