EncuA.01150.a

CASEIN KINASE II ALPHA CHAIN

CENTER ID: EncuA.01150.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.01150.a.A1.GE27829 Full length( EncuA.01150.a ) 1 319
EncuA.01150.a.MB1.GE30461 Full length( EncuA.01150.a ) 1 319
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU05_1510
RefSeq: NP_597494.1
UniProt: Q8SRU0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVVVTVARSN ADVNEKKDER YWNYECYTIT TGSIEKYQIY QRMGRGKYSE VFEGRKDREK IVIKALKPVR KAKICREVLI LRNLSHKNII KLMDVVVDPE SQIYSLIFEY IEHEDYAKIF EKLCYKDIVE YSRQILSALS YCHSMGIIHR DIKPQNMVIN QARRELKIID WGLAEFYHPK KEYSVRVASR YYKGPELLVD YPYYDYSLDI WSFGCVLAEL VFKKRPFFHG ESNSDQLVKI ARILGYTHLK KYVRKYKMAP LNPKYEGVGE RVLLSSFTPP GKTGLYTNAI DLLEKILIYD HQDRPTADEC LRHPLFESR
NT Sequence
ATGGTTGTTG TAACTGTAGC AAGGTCAAAC GCAGATGTTA ATGAAAAGAA GGATGAGAGG TACTGGAACT ATGAATGCTA TACCATAACC ACTGGAAGCA TAGAGAAATA CCAAATATAT CAGCGCATGG GCCGGGGAAA GTATTCGGAG GTGTTCGAAG GCCGCAAGGA CAGGGAGAAA ATTGTGATAA AAGCCCTTAA ACCCGTAAGG AAAGCAAAGA TCTGCCGGGA GGTGCTAATA CTGAGGAATC TTTCACACAA GAACATCATA AAATTGATGG ACGTTGTTGT TGACCCTGAA TCCCAAATAT ACTCCCTGAT TTTTGAATAC ATAGAGCATG AGGACTATGC AAAAATATTT GAGAAGCTGT GCTACAAAGA CATCGTTGAA TACTCCAGGC AAATTCTTTC CGCTCTAAGT TACTGCCATA GCATGGGAAT AATTCACAGG GACATCAAGC CACAGAACAT GGTGATTAAC CAAGCCAGGA GAGAGCTTAA AATAATTGAT TGGGGACTTG CAGAGTTTTA CCATCCAAAG AAAGAGTACA GCGTCAGAGT TGCAAGTAGA TACTACAAAG GCCCCGAGCT ATTGGTTGAT TACCCGTATT ACGACTATTC GCTTGACATC TGGTCGTTTG GATGCGTTTT GGCAGAGCTA GTGTTTAAGA AAAGGCCCTT TTTCCATGGG GAGAGCAACA GTGACCAGCT GGTCAAGATA GCCAGAATCC TAGGATACAC TCATCTGAAA AAGTACGTCA GAAAATACAA AATGGCTCCT CTTAATCCAA AATACGAGGG AGTGGGTGAG AGAGTGTTGC TTTCTTCCTT TACTCCTCCT GGAAAGACCG GTTTATACAC CAATGCAATA GACCTGCTAG AAAAAATACT AATATATGAC CACCAAGACA GGCCTACTGC TGATGAGTGC TTAAGACACC CTCTCTTTGA GTCCAGA
Details for EncuA.01150.a.A1.GE27829
HARVESTED ON: 11/3/2009
SEQUENCED ON: 1/13/2010
EXPECTED MW: 39kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMVVVTVA RSNADVNEKK DERYWNYECY TITTGSIEKY QIYQRMGRGK YSEVFEGRKD REKIVIKALK PVRKAKICRE VLILRNLSHK NIIKLMDVVV DPESQIYSLI FEYIEHEDYA KIFEKLCYKD IVEYSRQILS ALSYCHSMGI IHRDIKPQNM VINQARRELK IIDWGLAEFY HPKKEYSVRV ASRYYKGPEL LVDYPYYDYS LDIWSFGCVL AELVFKKRPF FHGESNSDQL VKIARILGYT HLKKYVRKYK MAPLNPKYEG VGERVLLSSF TPPGKTGLYT NAIDLLEKIL IYDHQDRPTA DECLRHPLFE SR
Validated NT Sequence
tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggttgt tgtaactgta gcaaggtcaa acgcagatgt taatgaaaag aaggatgaga ggtactggaa ctatgaatgc tataccataa ccactggaag catagagaaa taccaaatat atcagcgcat gggccgggga aagtattcgg aggtgttcga aggccgcaag gacagggaga aaattgtgat aaaagccctt aaacccgtaa ggaaagcaaa gatctgccgg gaggtgctaa tactgaggaa tctttcacac aagaacatca taaaattgat ggacgttgtt gttgaccctg aatcccaaat atactccctg atttttgaat acatagagca tgaggactat gcaaaaatat ttgagaagct gtgctacaaa gacatcgttg aatactccag gcaaattctt tccgctctaa gttactgcca tagcatggga ataattcaca gggacatcaa gccacagaac atggtgatta accaagccag gagagagctt aaaataattg attggggact tgcagagttt taccatccaa agaaagagta cagcgtcaga gttgcaagta gatactacaa aggccccgag ctattggttg attacccgta ttacgactat tcgcttgaca tctggtcgtt tggatgcgtt ttggcagagc tagtgtttaa gaaaaggccc tttttccatg gggagagcaa cagtgaccag ctggtcaaga tagccagaat cctaggatac actcatctga aaaagtacgt cagaaaatac aaaatggctc ctcttaatcc aaaatacgag ggagtgggtg agagagtgtt gctttcttcc tttactcctc ctggaaagac cggtttatac accaatgcaa tagacctgct agaaaaaata ctaatatatg accaccaaga caggcctact gctgatgagt gcttaagaca ccctctcttt gagtccagat aataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVVVTVARS NADVNEKKDE RYWNYECYTI TTGSIEKYQI YQRMGRGKYS EVFEGRKDRE KIVIKALKPV RKAKICREVL ILRNLSHKNI IKLMDVVVDP ESQIYSLIFE YIEHEDYAKI FEKLCYKDIV EYSRQILSAL SYCHSMGIIH RDIKPQNMVI NQARRELKII DWGLAEFYHP KKEYSVRVAS RYYKGPELLV DYPYYDYSLD IWSFGCVLAE LVFKKRPFFH GESNSDQLVK IARILGYTHL KKYVRKYKMA PLNPKYEGVG ERVLLSSFTP PGKTGLYTNA IDLLEKILIY DHQDRPTADE CLRHPLFESR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggttgttgta actgtagcaa ggtcaaacgc agatgttaat gaaaagaagg atgagaggta ctggaactat gaatgctata ccataaccac tggaagcata gagaaatacc aaatatatca gcgcatgggc cggggaaagt attcggaggt gttcgaaggc cgcaaggaca gggagaaaat tgtgataaaa gcccttaaac ccgtaaggaa agcaaagatc tgccgggagg tgctaatact gaggaatctt tcacacaaga acatcataaa attgatggac gttgttgttg accctgaatc ccaaatatac tccctgattt ttgaatacat agagcatgag gactatgcaa aaatatttga gaagctgtgc tacaaagaca tcgttgaata ctccaggcaa attctttccg ctctaagtta ctgccatagc atgggaataa ttcacaggga catcaagcca cagaacatgg tgattaacca agccaggaga gagcttaaaa taattgattg gggacttgca gagttttacc atccaaagaa agagtacagc gtcagagttg caagtagata ctacaaaggc cccgagctat tggttgatta cccgtattac gactattcgc ttgacatctg gtcgtttgga tgcgttttgg cagagctagt gtttaagaaa aggccctttt tccatgggga gagcaacagt gaccagctgg tcaagatagc cagaatccta ggatacactc atctgaaaaa gtacgtcaga aaatacaaaa tggctcctct taatccaaaa tacgagggag tgggtgagag agtgttgctt tcttccttta ctcctcctgg aaagaccggt ttatacacca atgcaataga cctgctagaa aaaatactaa tatatgacca ccaagacagg cctactgctg atgagtgctt aagacaccct ctctttgagt ccagaaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for EncuA.01150.a.MB1.GE30461
HARVESTED ON: 5/11/2010
SEQUENCED ON: 10/7/2010
EXPECTED MW: 82kDa
OBSERVED MW: 80kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 91
PERCENT COVERAGE: 43
Validated AA Sequence
MVVVTVARSN ADVNEKKDER YWNYECYTIT TGSIEXXXIY QRMGRGKYSE VFEGRKDREK IVIKALKPVR KAKICREVLI LRNLSHKNII KLMDVVVDPE SQIYSLIFEY IEHEDYAKIF EKLCYKDIVE YSRQILSALS YCHSMGIIHR DIKPQNMVIN QARRELKIID WGLAEFYHPK KEYGVRVASR YYKGPELLVD YPYYDYSLDI WSFGCVLAEL VFKKRPFFHG ESNSDQLVKI ARILXYTHLK KYVRKYKMAX LNPKYEGVGE RVLLSXFTXP GKTXLXTXXI DLLEKXTNIX XQXRXTXDEX XXXPXX
Validated NT Sequence
ctcngacccn gggtcctggt tcgatggttg ttgtaactgt agcaaggtca aacgcagatg ttaatgaaaa gaaggatgag aggtactgga actatgaatg ctataccata accactggaa gcatagagnn nannnaaata tatcagcgca tgggccgggg aaagtattcg gaggtgttcg aaggccgcaa ggacagggag aaaattgtga taaaagccct taaacccgta aggaaagcaa agatctgccg ggaggtgcta atactgagga atctttcaca caagaacatc ataaaattga tggacgttgt tgttgaccct gaatcccaaa tatactccct gatttttgaa tacatagagc atgaggacta tgcaaaaata tttgagaagc tgtgctacaa agacatcgtt gaatactcca ggcaaattct ttccgctcta agttactgcc atagcatggg aataattcac agggacatca agccacagaa catggtgatt aaccaagcca ggagagagct taaaataatt gattggggac ttgcagagtt ttaccatcca aagaaagagt acggcgtcag agttgcaagt agatactaca aaggccccga gctattggtt gattacccgt attacgacta ttcgcttgac atctggtcgt ttggatgcgt tttggcagag ctagtgttta agaaaaggcc ctttttccat ggggagagca acagtgacca gctggtcaag atagccagaa tcctangata cactcatctg aaaaagtacg tcagaaaata caaaatggct nctcttaatc caaaatacga gggagtgggn gagagagtgt tgctttctnn ctttactnct cctggaaaga ccgnnttata naccnntnnn atagacctgc tagaaaaaan tactaatatt ntganncaan acaggnctac tgntgatgag nnnttnagnn nnccnnnnnn ttgantccng annntaaana gcacgaacna nntnnncnnn nancttctcn angtncngnt gntnnnnaag nccnnnngnn nnnnngnnnn ntnnnnnnnn cnnnnnnnnn nnnnna
Expected Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS MVVVTVARSN ADVNEKKDER YWNYECYTIT TGSIEKYQIY QRMGRGKYSE VFEGRKDREK IVIKALKPVR KAKICREVLI LRNLSHKNII KLMDVVVDPE SQIYSLIFEY IEHEDYAKIF EKLCYKDIVE YSRQILSALS YCHSMGIIHR DIKPQNMVIN QARRELKIID WGLAEFYHPK KEYSVRVASR YYKGPELLVD YPYYDYSLDI WSFGCVLAEL VFKKRPFFHG ESNSDQLVKI ARILGYTHLK KYVRKYKMAP LNPKYEGVGE RVLLSSFTPP GKTGLYTNAI DLLEKILIYD HQDRPTADEC LRHPLFESR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcgatggt tgttgtaact gtagcaaggt caaacgcaga tgttaatgaa aagaaggatg agaggtactg gaactatgaa tgctatacca taaccactgg aagcatagag aaataccaaa tatatcagcg catgggccgg ggaaagtatt cggaggtgtt cgaaggccgc aaggacaggg agaaaattgt gataaaagcc cttaaacccg taaggaaagc aaagatctgc cgggaggtgc taatactgag gaatctttca cacaagaaca tcataaaatt gatggacgtt gttgttgacc ctgaatccca aatatactcc ctgatttttg aatacataga gcatgaggac tatgcaaaaa tatttgagaa gctgtgctac aaagacatcg ttgaatactc caggcaaatt ctttccgctc taagttactg ccatagcatg ggaataattc acagggacat caagccacag aacatggtga ttaaccaagc caggagagag cttaaaataa ttgattgggg acttgcagag ttttaccatc caaagaaaga gtacagcgtc agagttgcaa gtagatacta caaaggcccc gagctattgg ttgattaccc gtattacgac tattcgcttg acatctggtc gtttggatgc gttttggcag agctagtgtt taagaaaagg ccctttttcc atggggagag caacagtgac cagctggtca agatagccag aatcctagga tacactcatc tgaaaaagta cgtcagaaaa tacaaaatgg ctcctcttaa tccaaaatac gagggagtgg gtgagagagt gttgctttct tcctttactc ctcctggaaa gaccggttta tacaccaatg caatagacct gctagaaaaa atactaatat atgaccacca agacaggcct actgctgatg agtgcttaag acaccctctc tttgagtcca gataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa