EncuA.01101.a

ATP-DEPENDENT RNA HELICASE

CENTER ID: EncuA.01101.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.01101.a.A1.GE27827 Full length( EncuA.01101.a ) 1 426
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU05_1270
OrthoMCL: OG5_230698
RefSeq: NP_597470.1
UniProt: Q8SRV1
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIKLIQEYLS FVSSPMRNPL VLRSLKNKIL EKTKSRKQMA CTVDTPRPWR SPRIVSNGSK EPLTEWPSSF NKGLLPPHPT DVQRFVIPYI LAGEGVDVIS ETGSGKTLSY VLPHIHLLES GDKRLLVIVP TRELVSQVGQ VFRKFSEGLL RIVEITGEVS IESQRLQVSR PFDVAISTPG RLRELLELKS IGGFEYVVVD EADRLMQHDI RSDVTFILER MKPEVASFFS ATPFEWSGGT RILVGNVSVG RDVEEFFMYV EKKEKMQVLG EILVSCEGWL AKNMGVDRNG LEVKKIIVFC NSIRTCENLH KRFRQLLVLH SRRTMQERKA VIEALEGERA VLIATDLGGR GIDIKDVDLV VNYDLPRAVD GYVHRCGRTG RQRKGMALSM ICREDREILP KLKRLVEKRG GTVPGFMNVK EDVILD
NT Sequence
ATGATTAAAC TAATCCAGGA ATATTTGTCT TTTGTGTCAT CCCCAATGAG GAATCCTTTG GTGCTCAGGA GTTTGAAAAA CAAGATTCTC GAAAAGACTA AGAGTAGGAA GCAGATGGCC TGTACTGTCG ATACCCCAAG GCCTTGGCGG TCTCCTAGGA TTGTTTCGAA TGGCAGCAAA GAACCTCTGA CGGAATGGCC TTCCTCGTTC AACAAGGGGT TACTTCCTCC GCATCCGACA GACGTCCAGA GGTTCGTGAT TCCGTATATC CTGGCAGGAG AGGGCGTTGA TGTTATTTCC GAGACCGGAA GCGGGAAGAC CCTTTCCTAC GTACTGCCAC ACATCCATCT TCTTGAAAGC GGAGACAAAA GACTACTGGT CATTGTTCCC ACAAGGGAGC TAGTTTCACA GGTAGGTCAA GTATTCAGAA AGTTTTCGGA AGGCCTCCTT AGGATTGTGG AAATAACGGG GGAGGTAAGC ATAGAGTCGC AACGTCTCCA GGTTTCAAGG CCATTTGATG TGGCCATTTC CACGCCCGGA AGGCTGAGAG AGCTTCTGGA ATTAAAGAGC ATTGGAGGCT TCGAATATGT TGTTGTCGAT GAGGCCGATA GACTGATGCA GCACGACATC AGGAGCGACG TGACTTTTAT ACTAGAAAGG ATGAAGCCAG AGGTCGCCAG CTTTTTTTCT GCAACGCCTT TTGAATGGTC TGGGGGGACA AGGATTTTGG TTGGAAACGT TTCTGTTGGC CGAGATGTGG AGGAATTCTT TATGTACGTT GAGAAGAAAG AAAAGATGCA GGTTCTTGGG GAGATTCTAG TTTCTTGTGA GGGCTGGCTC GCAAAGAACA TGGGTGTGGA TAGGAATGGC TTAGAAGTAA AAAAAATAAT TGTGTTCTGC AACAGCATCA GGACGTGTGA AAATCTTCAC AAGAGGTTCA GGCAGTTGCT AGTACTTCAC TCAAGAAGGA CCATGCAGGA GAGGAAGGCT GTCATCGAAG CATTAGAGGG AGAAAGAGCA GTCTTGATAG CTACCGATTT AGGGGGAAGA GGCATTGATA TAAAGGATGT GGATCTGGTA GTAAACTATG ATCTTCCCCG GGCCGTTGAT GGCTATGTAC ATAGGTGCGG GAGAACTGGA AGGCAAAGAA AGGGAATGGC TCTGTCAATG ATATGTCGAG AGGACAGGGA AATACTCCCC AAGCTCAAAA GACTTGTTGA GAAGAGGGGG GGTACTGTGC CTGGGTTTAT GAATGTAAAG GAGGATGTAA TATTGGAC
Details for EncuA.01101.a.A1.GE27827
HARVESTED ON: 11/3/2009
SEQUENCED ON: 11/10/2009
EXPECTED MW: 50kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MGTLEAQTQG PGSMIKLIQE YLSFVSSPMR NPLVLRSLKN KILEKTKSRK QMACTVDTPR PWRSPRIVSN GSKEPLTEWP SSFNKGLLPP HPTDVQRFVI PYILAGEGVD VISETGSGKT LSYVLPHIHL LESGDKRLLV IVPTRELVSQ VGQVFRKFSE GLLRIVEITG EVSIESQRLQ VSRPFDVAIS TPGRLRELLE LKSIGGFEYV VVDEADRLMQ HDIRSDVTFI LERMKPEVAS FFSATPFEWS GGTRILVGNV SVGRDVEEFF MYVEKKEKMQ VLGEILVSCE GWLAKNMGVD RNGLEVKKII VFCNSIRTCE NLHKRFRQLL VLHSRRTMQE RKAVIEALEG ERAVLIATDL GGRGIDIKDV DLVVNYDLPR AVDGYVHRCG RTGRQRKGMA LSMICREDRE ILPKLKRLVE KRGGTVPGFM NVKEDVILD
Validated NT Sequence
attagtccaa tattacatcc tcctttacat tcataaaccc aggcacagta ccccccctct tctcaacaag tcttttgagc ttggggagta tttccctgtc ctctcgacat atcattgaca gagccattcc ctttctttgc cttccagttc tcccgcacct atgtacatag ccatcaacgg cccggggaag atcatagttt actaccagat ccacatcctt tatatcaatg cctcttcccc ctaaatcggt agctatcaag actgctcttt ctccctctaa tgcttcgatg acagccttcc tctcctgcat ggtccttctt gagtgaagta ctagcaactg cctgaacctc ttgtgaagat tttcacacgt cctgatgctg ttgcagaaca caattatttt ttttacttct aagccattcc tatccacacc catgttcttt gcgagccagc cctcacaaga aactagaatc tccccaagaa cctgcatctt ttctttcttc tcaacgtaca taaagaattc ctccacatct cggccaacag aaacgtttcc aaccaaaatc cttgtccccc cagaccattc aaaaggcgtt gcagaaaaaa agctggcgac ctctggcttc atcctttcta gtataaaagt cacgtcgctc ctgatgtcgt gctgcatcag tctatcggcc tcatcgacaa caacatattc gaagcctcca atgctcttta attccagaag ctctctcagc cttccgggcg tggaaatggc cacatcaaat ggccttgaaa cctggagacg ttgcgactct atgcttacct cccccgttat ttccacaatc ctaaggaggc cttccgaaaa ctttctgaat acttgaccta cctgtgaaac tagctccctt gtgggaacaa tgaccagtag tcttttgtct ccgctttcaa gaagatggat gtgtggcagt acgtaggaaa gggtcttccc gcttccggtc tcggaaataa catcaacgcc ctctcctgcc aggatatacg gaatcacgaa cctctggacg tctgtcggat gcggaggaag taaccccttg ttgaacgagg aaggccattc cgtcagaggt tctttgctgc cattcgaaac aatcctagga gaccgccaag gccttggggt atcgacagta caggccatct gcttcctact cttagtcttt tcgagaatct tgtttttcaa actcctgagc accaaaggat tcctcattgg ggatgacaca aaagacaaat attcctggat tagtttaatc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIKLIQEYL SFVSSPMRNP LVLRSLKNKI LEKTKSRKQM ACTVDTPRPW RSPRIVSNGS KEPLTEWPSS FNKGLLPPHP TDVQRFVIPY ILAGEGVDVI SETGSGKTLS YVLPHIHLLE SGDKRLLVIV PTRELVSQVG QVFRKFSEGL LRIVEITGEV SIESQRLQVS RPFDVAISTP GRLRELLELK SIGGFEYVVV DEADRLMQHD IRSDVTFILE RMKPEVASFF SATPFEWSGG TRILVGNVSV GRDVEEFFMY VEKKEKMQVL GEILVSCEGW LAKNMGVDRN GLEVKKIIVF CNSIRTCENL HKRFRQLLVL HSRRTMQERK AVIEALEGER AVLIATDLGG RGIDIKDVDL VVNYDLPRAV DGYVHRCGRT GRQRKGMALS MICREDREIL PKLKRLVEKR GGTVPGFMNV KEDVILD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gattaaacta atccaggaat atttgtcttt tgtgtcatcc ccaatgagga atcctttggt gctcaggagt ttgaaaaaca agattctcga aaagactaag agtaggaagc agatggcctg tactgtcgat accccaaggc cttggcggtc tcctaggatt gtttcgaatg gcagcaaaga acctctgacg gaatggcctt cctcgttcaa caaggggtta cttcctccgc atccgacaga cgtccagagg ttcgtgattc cgtatatcct ggcaggagag ggcgttgatg ttatttccga gaccggaagc gggaagaccc tttcctacgt actgccacac atccatcttc ttgaaagcgg agacaaaaga ctactggtca ttgttcccac aagggagcta gtttcacagg taggtcaagt attcagaaag ttttcggaag gcctccttag gattgtggaa ataacggggg aggtaagcat agagtcgcaa cgtctccagg tttcaaggcc atttgatgtg gccatttcca cgcccggaag gctgagagag cttctggaat taaagagcat tggaggcttc gaatatgttg ttgtcgatga ggccgataga ctgatgcagc acgacatcag gagcgacgtg acttttatac tagaaaggat gaagccagag gtcgccagct ttttttctgc aacgcctttt gaatggtctg gggggacaag gattttggtt ggaaacgttt ctgttggccg agatgtggag gaattcttta tgtacgttga gaagaaagaa aagatgcagg ttcttgggga gattctagtt tcttgtgagg gctggctcgc aaagaacatg ggtgtggata ggaatggctt agaagtaaaa aaaataattg tgttctgcaa cagcatcagg acgtgtgaaa atcttcacaa gaggttcagg cagttgctag tacttcactc aagaaggacc atgcaggaga ggaaggctgt catcgaagca ttagagggag aaagagcagt cttgatagct accgatttag ggggaagagg cattgatata aaggatgtgg atctggtagt aaactatgat cttccccggg ccgttgatgg ctatgtacat aggtgcggga gaactggaag gcaaagaaag ggaatggctc tgtcaatgat atgtcgagag gacagggaaa tactccccaa gctcaaaaga cttgttgaga agaggggggg tactgtgcct gggtttatga atgtaaagga ggatgtaata ttggacaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa