SSGCID: Target Page

EncuA.01098.a

HISTONE DEACETYLASE 1

CENTER ID:	EncuA.01098.a
ORGANISM:	Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE:	Microsporidiosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EncuA.01098.a.A1.GE30654	Full length( EncuA.01098.a )		1	416

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	MicrosporidiaDB:ECU03_1370
RefSeq:	NP_597645.1
UniProt:	Q8SS80

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNNKRVVYFY DPEIGCFSYA MGHPMKPLRV KMTHTLLVGY ELYKYMDVLR PFQASYENLT NFHAVDYINF LSSVSSENME EMIKDLHKFN IRDDCPVFPG LYDYCRLTSG GSIYAAQKIN SGKYDVAINW AGGLHHAKRG EASGFCYVND IVLGILELLK YNKRILYIDI DIHHGDGVEE AFYTTDRVMT VSFHKYGDYF PGTGMVTDTG LAKGKGYSVN VPLKDGIDDE SYFSIFKPIV SKVIETYQPN AIVLQSGADS LSGDKLGCFN LSHLGHSRCI KFVQSFNIPL ILLGGGGYTI GNVSKAWAYG TSVVLDVDIP REIPYNEYFD YYAPTYRIDV PTSNMANQNT REGLEDIIAK VYENLRSLSH APSVQMVSIP PSFISDESDE DEVSKKKAEA EDSDDYKAFS DSKDED

NT Sequence

ATGAACAATA AGAGAGTGGT TTACTTCTAT GACCCCGAAA TAGGATGCTT CAGCTACGCA ATGGGACATC CAATGAAGCC TCTGAGAGTG AAAATGACCC ATACTCTTCT TGTTGGGTAT GAGCTTTACA AATACATGGA TGTTCTCAGG CCCTTTCAGG CATCGTACGA GAACCTGACG AACTTCCACG CGGTAGACTA CATAAACTTC CTTTCAAGCG TCTCCAGCGA AAACATGGAG GAGATGATTA AGGACCTCCA CAAGTTCAAC ATCAGAGACG ACTGCCCTGT GTTTCCGGGG CTCTACGACT ATTGCAGACT CACGAGCGGA GGGAGTATTT ATGCAGCCCA GAAAATAAAC TCCGGGAAGT ATGATGTTGC AATCAACTGG GCTGGAGGGC TGCACCATGC GAAGAGAGGA GAAGCCTCTG GATTTTGCTA TGTAAACGAC ATAGTTCTAG GGATTCTCGA GCTCCTGAAG TACAACAAAA GAATTCTGTA CATAGATATA GACATTCATC ACGGAGACGG GGTTGAGGAG GCATTCTATA CCACCGACAG GGTCATGACA GTCTCCTTCC ACAAGTATGG AGACTATTTC CCAGGGACAG GGATGGTCAC CGACACCGGC CTGGCAAAGG GAAAGGGCTA TTCGGTCAAC GTTCCACTGA AGGACGGGAT TGACGACGAA AGCTACTTCA GTATATTCAA GCCCATTGTC AGCAAGGTCA TTGAGACGTA CCAGCCAAAT GCAATTGTAC TGCAGTCTGG GGCAGACTCC CTTTCTGGAG ACAAGCTAGG GTGCTTCAAT CTGTCGCACC TGGGGCATTC AAGGTGTATT AAATTTGTGC AGAGCTTCAA CATCCCACTC ATCCTTCTGG GAGGAGGTGG GTACACCATT GGAAATGTCT CGAAGGCATG GGCATACGGG ACAAGCGTGG TTCTGGATGT GGATATCCCC AGGGAAATCC CCTACAACGA GTATTTTGAT TACTATGCAC CGACATACAG GATAGATGTT CCTACATCAA ACATGGCAAA CCAGAACACA AGAGAAGGCC TAGAAGACAT TATAGCGAAG GTCTATGAGA ATCTGAGGAG CCTTTCTCAT GCACCATCGG TACAAATGGT CTCCATTCCT CCGTCCTTCA TCAGCGACGA AAGCGACGAG GACGAGGTTT CCAAGAAGAA GGCTGAAGCA GAAGACTCAG ACGATTACAA AGCATTCTCT GATTCAAAGG ACGAGGAC

Details for EncuA.01098.a.A1.GE30654

HARVESTED ON:	10/27/2010
SEQUENCED ON:	1/13/2011
EXPECTED MW:	49kDa
OBSERVED MW:	49kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMNNKRVVYF YDPEIGCFSY AMGHPMKPLR VKMTHTLLVG YELYKYMDVL RPFQASYENL TNFHAVDYIN FLSSVSSENM EEMIKDLHKF NIRDDCPVFP GLYDYCRLTS GGSIYAAQKI NSGKYDVAIN WAGGLHHAKR GEASGFCYVN DIVLGILELL KYNKRILYID IDIHHGDGVE EAFYTTDRVM TVSFHKYGDY FPGTGMVTDT GLAKGKGYSV NVPLKDGIDD ESYFSIFKPI VSKVIETYQP NAIVLQSGAD SLSGDKLGCF NLSHLGHSRC IKFVQSFNIP LILLGGGGYT IGNVSKAWAY GTSVVLDVDI PREIPYNEYF DYYAPTYRID VPTSNMANQN TREGLEDIIA KVYENLRSLS HAPSVQMVSI PPSFISDESD EDEVSKKKAE AEDSDDYKA

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaca ataagagagt ggtttacttc tatgaccccg aaataggatg cttcagctac gcaatgggac atccaatgaa gcctctgaga gtgaaaatga cccatactct tcttgttggg tatgagcttt acaaatacat ggatgttctc aggccctttc aggcatcgta cgagaacctg acgaacttcc acgcggtaga ctacataaac ttcctttcaa gcgtctccag cgaaaacatg gaggagatga ttaaggacct ccacaagttc aacatcagag acgactgccc tgtgtttccg gggctctacg actattgcag actcacgagc ggagggagta tttatgcagc ccagaaaata aactccggga agtatgatgt tgcaatcaac tgggctggag ggctgcacca tgcgaagaga ggagaagcct ctggattttg ctatgtaaac gacatagttc tagggattct cgagctcctg aagtacaaca aaagaattct gtacatagat atagacattc atcacggaga cggggttgag gaggcattct ataccaccga cagggtcatg acagtctcct tccacaagta tggagactat ttcccaggga cagggatggt caccgacacc ggcctggcaa agggaaaggg ctattcggtc aacgttccac tgaaggacgg gattgacgac gaaagctact tcagtatatt caagcccatt gtcagcaagg tcattgagac gtaccagcca aatgcaattg tactgcagtc tggggcagac tccctttctg gagacaagct agggtgcttc aatctgtcgc acctggggca ttcaaggtgt attaaatttg tgcagagctt caacatccca ctcatccttc tgggaggagg tgggtacacc attggaaatg tctcgaaggc atgggcatac gggacaagcg tggttctgga tgtggatatc cccagggaaa tcccctacaa cgagtatttt gattactatg caccgacata caggatagat gttcctacat caaacatggc aaaccagaac acaagagaag gcctagaaga cattatagcg aaggtctatg agaatctgag gagcctttct catgcaccat cggtacaaat ggtctccatt cctccgtcct tcatcagcga cgaaagcgac gaggacgagg tttccaagaa gaaggctgaa gcagaagact cagacgatta caaagcattc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaacaataag agagtggttt acttctatga ccccgaaata ggatgcttca gctacgcaat gggacatcca atgaagcctc tgagagtgaa aatgacccat actcttcttg ttgggtatga gctttacaaa tacatggatg ttctcaggcc ctttcaggca tcgtacgaga acctgacgaa cttccacgcg gtagactaca taaacttcct ttcaagcgtc tccagcgaaa acatggagga gatgattaag gacctccaca agttcaacat cagagacgac tgccctgtgt ttccggggct ctacgactat tgcagactca cgagcggagg gagtatttat gcagcccaga aaataaactc cgggaagtat gatgttgcaa tcaactgggc tggagggctg caccatgcga agagaggaga agcctctgga ttttgctatg taaacgacat agttctaggg attctcgagc tcctgaagta caacaaaaga attctgtaca tagatataga cattcatcac ggagacgggg ttgaggaggc attctatacc accgacaggg tcatgacagt ctccttccac aagtatggag actatttccc agggacaggg atggtcaccg acaccggcct ggcaaaggga aagggctatt cggtcaacgt tccactgaag gacgggattg acgacgaaag ctacttcagt atattcaagc ccattgtcag caaggtcatt gagacgtacc agccaaatgc aattgtactg cagtctgggg cagactccct ttctggagac aagctagggt gcttcaatct gtcgcacctg gggcattcaa ggtgtattaa atttgtgcag agcttcaaca tcccactcat ccttctggga ggaggtgggt acaccattgg aaatgtctcg aaggcatggg catacgggac aagcgtggtt ctggatgtgg atatccccag ggaaatcccc tacaacgagt attttgatta ctatgcaccg acatacagga tagatgttcc tacatcaaac atggcaaacc agaacacaag agaaggccta gaagacatta tagcgaaggt ctatgagaat ctgaggagcc tttctcatgc accatcggta caaatggtct ccattcctcc gtccttcatc agcgacgaaa gcgacgagga cgaggtttcc aagaagaagg ctgaagcaga agactcagac gattacaaag cattctctga ttcaaaggac gaggacaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa