EncuA.01002.a
GTP-BINDING NUCLEAR PROTEIN
| CENTER ID: | EncuA.01002.a |
| ORGANISM: | Encephalitozoon cuniculi GB-M1 |
| ASSOCIATED DISEASE: | Microsporidiosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I/IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| EncuA.01002.a.A1.GE27821 | Full length( EncuA.01002.a ) | 1 | 214 | ||
| EncuA.01002.a.A1.GE28335 | Full length( EncuA.01002.a ) | 1 | 214 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| EncuA.01002.a.A1.PS00532 | Full length( EncuA.01002.a ) | 1 | 214 |
Structures
4DJT
DEPOSITED: 2/2/2012
DETERMINATION: XRay
CLONE: EncuA.01002.a.A1.GE27821
PROTEIN: EncuA.01002.a.A1.PS00532
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | MicrosporidiaDB:ECU04_1560 |
| OrthoMCL: | OG5_127514 |
| RefSeq: | NP_584841.1 |
| UniProt: | Q8SS11 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MERRELTYKI CLIGDGGVGK TTYINRVLDG RFEKNYNATV GAVNHPVTFL DDQGNVIKFN VWDTAGQEKK AVLKDVYYIG ASGAIFFFDV TSRITCQNLA RWVKEFQAVV GNEAPIVVCA NKIDIKNRQK ISKKLVMEVL KGKNYEYFEI SAKTAHNFGL PFLHLARIFT GRPDLIFVSN VNLEPTEVNY DYHSPEESKY IDYMEQASKM APEE
NT Sequence
ATGGAGAGGA GAGAGTTAAC ATACAAGATA TGTCTGATAG GGGATGGAGG AGTTGGAAAA ACAACGTACA TAAACAGGGT GCTTGATGGA AGGTTTGAAA AGAACTACAA TGCAACAGTT GGAGCAGTGA ATCACCCCGT GACGTTTCTT GACGACCAAG GAAATGTAAT AAAGTTTAAT GTGTGGGACA CTGCGGGGCA GGAGAAGAAG GCGGTTCTGA AGGACGTATA CTACATAGGA GCCAGCGGAG CCATCTTCTT CTTTGATGTG ACGTCGAGGA TCACGTGCCA GAACCTTGCG CGATGGGTTA AGGAGTTCCA AGCGGTTGTT GGTAACGAGG CACCTATAGT CGTCTGTGCT AACAAGATCG ACATAAAGAA TAGGCAGAAG ATTAGTAAGA AGCTGGTGAT GGAGGTGTTG AAGGGAAAGA ACTACGAGTA CTTTGAGATC AGCGCGAAGA CTGCGCATAA CTTCGGGCTT CCGTTTTTGC ACCTGGCAAG GATCTTTACC GGAAGGCCGG ATCTTATATT TGTGTCGAAT GTGAATCTTG AGCCAACCGA AGTCAATTAC GACTATCATA GTCCTGAGGA GTCGAAATAC ATTGACTACA TGGAGCAGGC GTCGAAGATG GCTCCAGAGG AG
Details for EncuA.01002.a.A1.GE27821
| HARVESTED ON: | 11/3/2009 |
| SEQUENCED ON: | 11/10/2009 |
| EXPECTED MW: | 26kDa |
| OBSERVED MW: | 29kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with sequence variation |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMERRELTYK ICLIGDGGVG KTTYINRVLD GRFEKNYNAT VGAVNHPVTF LDDQGNVIKF NVWDTAGQEK KAVLKDVYYI GASGAILFFD VTSRITCQNL ARWVKEFQAV VGNEAPIVVC ANKIDIKNRQ KISKKLVMEV LKGKNYEYFE ISAKTAHNFG LPFLHLARIF TGRPDLIFVS NVNLEPTEVN YDYHSPEESK YIDYMEQASK MAPEE
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggaga ggagagagtt aacatacaag atatgtctga taggggatgg aggagttgga aaaacaacgt acataaacag ggtgcttgat ggaaggtttg aaaagaacta caatgcaaca gttggagcag tgaatcaccc cgtgacgttt cttgacgacc aaggaaatgt aataaagttt aatgtgtggg acactgcggg gcaggagaag aaggcggttc tgaaggacgt atactacata ggagccagcg gagccatcct cttctttgat gtgacgtcga ggatcacgtg ccagaacctt gcgcgatggg ttaaggagtt ccaagcggtt gttggtaacg aggcacctat agtcgtctgt gctaacaaga tcgacataaa gaataggcag aagattagta agaagctggt gatggaggtg ttgaagggaa agaactacga gtactttgag atcagcgcga agactgcgca taacttcggg cttccgtttt tgcacctggc aaggatcttt accggaaggc cggatcttat atttgtgtcg aatgtgaatc ttgagccaac cgaagtcaat tacgactatc atagtcctga ggagtcgaaa tacattgact acatggagca ggcgtcgaag atggctccag aggagtaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMERRELTYK ICLIGDGGVG KTTYINRVLD GRFEKNYNAT VGAVNHPVTF LDDQGNVIKF NVWDTAGQEK KAVLKDVYYI GASGAIFFFD VTSRITCQNL ARWVKEFQAV VGNEAPIVVC ANKIDIKNRQ KISKKLVMEV LKGKNYEYFE ISAKTAHNFG LPFLHLARIF TGRPDLIFVS NVNLEPTEVN YDYHSPEESK YIDYMEQASK MAPEE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggagaggaga gagttaacat acaagatatg tctgataggg gatggaggag ttggaaaaac aacgtacata aacagggtgc ttgatggaag gtttgaaaag aactacaatg caacagttgg agcagtgaat caccccgtga cgtttcttga cgaccaagga aatgtaataa agtttaatgt gtgggacact gcggggcagg agaagaaggc ggttctgaag gacgtatact acataggagc cagcggagcc atcttcttct ttgatgtgac gtcgaggatc acgtgccaga accttgcgcg atgggttaag gagttccaag cggttgttgg taacgaggca cctatagtcg tctgtgctaa caagatcgac ataaagaata ggcagaagat tagtaagaag ctggtgatgg aggtgttgaa gggaaagaac tacgagtact ttgagatcag cgcgaagact gcgcataact tcgggcttcc gtttttgcac ctggcaagga tctttaccgg aaggccggat cttatatttg tgtcgaatgt gaatcttgag ccaaccgaag tcaattacga ctatcatagt cctgaggagt cgaaatacat tgactacatg gagcaggcgt cgaagatggc tccagaggag aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for EncuA.01002.a.A1.GE28335
| HARVESTED ON: | 1/5/2010 |
| SEQUENCED ON: | 1/15/2010 |
| EXPECTED MW: | 26kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMERRELTYK ICLIGDGGVG KTTYINRVLD GRFEKNYNAT VGAVNHPVTF LDDQGNVIKF NVWDTAGQEK KAVLKDVYYI GASGAILFFD VTSRITCQNL ARWVKEFQAV VGNEAPIVVC ANKIDIKNRQ KISKKLVMEV LKGKNYEYFE ISAKTAHNFG LPFLHLARIF TGRPDLIFVS NVNLEPTEVN YDYHSPEESK YIDYMEQASK MAPEE
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggaga ggagagagtt aacatacaag atatgtctga taggggatgg aggagttgga aaaacaacgt acataaacag ggtgcttgat ggaaggtttg aaaagaacta caatgcaaca gttggagcag tgaatcaccc cgtgacgttt cttgacgacc aaggaaatgt aataaagttt aatgtgtggg acactgcggg gcaggagaag aaggcggttc tgaaggacgt atactacata ggagccagcg gagccatcct cttctttgat gtgacgtcga ggatcacgtg ccagaacctt gcgcgatggg ttaaggagtt ccaagcggtt gttggtaacg aggcacctat agtcgtctgt gctaacaaga tcgacataaa gaataggcag aagattagta agaagctggt gatggaggtg ttgaagggaa agaactacga gtactttgag atcagcgcga agactgcgca taacttcggg cttccgtttt tgcacctggc aaggatcttt accggaaggc cggatcttat atttgtgtcg aatgtgaatc ttgagccaac cgaagtcaat tacgactatc atagtcctga ggagtcgaaa tacattgact acatggagca ggcgtcgaag atggctccag aggagtaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMERRELTYK ICLIGDGGVG KTTYINRVLD GRFEKNYNAT VGAVNHPVTF LDDQGNVIKF NVWDTAGQEK KAVLKDVYYI GASGAIFFFD VTSRITCQNL ARWVKEFQAV VGNEAPIVVC ANKIDIKNRQ KISKKLVMEV LKGKNYEYFE ISAKTAHNFG LPFLHLARIF TGRPDLIFVS NVNLEPTEVN YDYHSPEESK YIDYMEQASK MAPEE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggagaggaga gagttaacat acaagatatg tctgataggg gatggaggag ttggaaaaac aacgtacata aacagggtgc ttgatggaag gtttgaaaag aactacaatg caacagttgg agcagtgaat caccccgtga cgtttcttga cgaccaagga aatgtaataa agtttaatgt gtgggacact gcggggcagg agaagaaggc ggttctgaag gacgtatact acataggagc cagcggagcc atcttcttct ttgatgtgac gtcgaggatc acgtgccaga accttgcgcg atgggttaag gagttccaag cggttgttgg taacgaggca cctatagtcg tctgtgctaa caagatcgac ataaagaata ggcagaagat tagtaagaag ctggtgatgg aggtgttgaa gggaaagaac tacgagtact ttgagatcag cgcgaagact gcgcataact tcgggcttcc gtttttgcac ctggcaagga tctttaccgg aaggccggat cttatatttg tgtcgaatgt gaatcttgag ccaaccgaag tcaattacga ctatcatagt cctgaggagt cgaaatacat tgactacatg gagcaggcgt cgaagatggc tccagaggag aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for EncuA.01002.a.A1.PS00532
| PURIFICATION DATe: | 3/10/2010 |
| CONCENTRATION: | 44.6mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMERRELTYK ICLIGDGGVG KTTYINRVLD GRFEKNYNAT VGAVNHPVTF LDDQGNVIKF NVWDTAGQEK KAVLKDVYYI GASGAILFFD VTSRITCQNL ARWVKEFQAV VGNEAPIVVC ANKIDIKNRQ KISKKLVMEV LKGKNYEYFE ISAKTAHNFG LPFLHLARIF TGRPDLIFVS NVNLEPTEVN YDYHSPEESK YIDYMEQASK MAPEE
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggaga ggagagagtt aacatacaag atatgtctga taggggatgg aggagttgga aaaacaacgt acataaacag ggtgcttgat ggaaggtttg aaaagaacta caatgcaaca gttggagcag tgaatcaccc cgtgacgttt cttgacgacc aaggaaatgt aataaagttt aatgtgtggg acactgcggg gcaggagaag aaggcggttc tgaaggacgt atactacata ggagccagcg gagccatcct cttctttgat gtgacgtcga ggatcacgtg ccagaacctt gcgcgatggg ttaaggagtt ccaagcggtt gttggtaacg aggcacctat agtcgtctgt gctaacaaga tcgacataaa gaataggcag aagattagta agaagctggt gatggaggtg ttgaagggaa agaactacga gtactttgag atcagcgcga agactgcgca taacttcggg cttccgtttt tgcacctggc aaggatcttt accggaaggc cggatcttat atttgtgtcg aatgtgaatc ttgagccaac cgaagtcaat tacgactatc atagtcctga ggagtcgaaa tacattgact acatggagca ggcgtcgaag atggctccag aggagtaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMERRELTYK ICLIGDGGVG KTTYINRVLD GRFEKNYNAT VGAVNHPVTF LDDQGNVIKF NVWDTAGQEK KAVLKDVYYI GASGAIFFFD VTSRITCQNL ARWVKEFQAV VGNEAPIVVC ANKIDIKNRQ KISKKLVMEV LKGKNYEYFE ISAKTAHNFG LPFLHLARIF TGRPDLIFVS NVNLEPTEVN YDYHSPEESK YIDYMEQASK MAPEE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGAGAGGAGA GAGTTAACAT ACAAGATATG TCTGATAGGG GATGGAGGAG TTGGAAAAAC AACGTACATA AACAGGGTGC TTGATGGAAG GTTTGAAAAG AACTACAATG CAACAGTTGG AGCAGTGAAT CACCCCGTGA CGTTTCTTGA CGACCAAGGA AATGTAATAA AGTTTAATGT GTGGGACACT GCGGGGCAGG AGAAGAAGGC GGTTCTGAAG GACGTATACT ACATAGGAGC CAGCGGAGCC ATCTTCTTCT TTGATGTGAC GTCGAGGATC ACGTGCCAGA ACCTTGCGCG ATGGGTTAAG GAGTTCCAAG CGGTTGTTGG TAACGAGGCA CCTATAGTCG TCTGTGCTAA CAAGATCGAC ATAAAGAATA GGCAGAAGAT TAGTAAGAAG CTGGTGATGG AGGTGTTGAA GGGAAAGAAC TACGAGTACT TTGAGATCAG CGCGAAGACT GCGCATAACT TCGGGCTTCC GTTTTTGCAC CTGGCAAGGA TCTTTACCGG AAGGCCGGAT CTTATATTTG TGTCGAATGT GAATCTTGAG CCAACCGAAG TCAATTACGA CTATCATAGT CCTGAGGAGT CGAAATACAT TGACTACATG GAGCAGGCGT CGAAGATGGC TCCAGAGGAG AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA