EncuA.00954.a

CELL DIVISION CONTROL PROTEIN 2 HOMOLOG

CENTER ID: EncuA.00954.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.00954.a.A1.GE27819 Full length( EncuA.00954.a ) 1 329
EncuA.00954.a.A1.GE28331 Full length( EncuA.00954.a ) 1 329
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU11_0960
RefSeq: NP_586402.1
UniProt: Q8SQW2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEPAEEGEIL AHRRFTATKM EYEKIRIIGE GTFGQVILAR KGRARYALKK VSKEKEGLSV TTIREVQVLR AMGHPSIVRL IEVVVEPGGD IYMVFPYFPY DLNRFIRSNK MTCSEIKHIF YQIAQGVCYI HSKGIMHRDL KSANILLDQK LNASIADFGM ARYTTKTGAY TPGMVTLWYR APEILLGSSS YTYAVDIWSL GCILTEMYLG HMIFQGSTEM LQLEMVIHAC GSINENSYPG VQDLPGFRNF RLPQSPRRIE GIIRKHDASA VELVSKMLCL DPSKRITVEQ VVGSKYFEHE ARRDASSAGL QGCSYREDRL SLSKRKNVD
NT Sequence
ATGGAGCCTG CTGAGGAAGG GGAGATCTTG GCACACAGAC GGTTCACTGC AACCAAGATG GAGTACGAGA AGATAAGAAT CATCGGGGAA GGCACCTTCG GACAGGTTAT TCTTGCAAGA AAGGGAAGGG CCAGATATGC ACTGAAGAAG GTAAGCAAGG AAAAGGAGGG ATTGTCGGTG ACAACGATCA GGGAGGTCCA GGTTCTGCGG GCAATGGGCC ATCCAAGCAT AGTAAGACTC ATAGAGGTTG TTGTCGAGCC TGGCGGAGAT ATCTACATGG TGTTCCCATA CTTTCCATAT GATCTGAACA GATTCATAAG AAGCAACAAG ATGACATGCA GCGAGATCAA GCACATATTC TATCAGATTG CTCAGGGAGT GTGCTACATC CACAGCAAGG GAATCATGCA CAGAGACCTG AAGAGTGCAA ACATTCTTCT GGACCAAAAG CTGAATGCAA GCATCGCAGA CTTTGGGATG GCAAGGTACA CAACAAAGAC AGGAGCATAC ACCCCAGGAA TGGTCACTTT GTGGTACAGG GCCCCGGAGA TACTCTTGGG GTCCTCCAGC TACACGTATG CTGTGGACAT CTGGTCATTG GGGTGCATAC TAACCGAGAT GTATCTGGGG CACATGATAT TCCAGGGAAG CACGGAGATG CTGCAGCTGG AGATGGTTAT CCATGCATGC GGATCCATCA ACGAAAACAG CTATCCAGGA GTGCAGGACC TCCCTGGATT CCGCAACTTC AGGCTTCCCC AGTCTCCCCG GAGAATAGAA GGCATCATCA GGAAGCATGA TGCGTCCGCA GTCGAGTTGG TCAGCAAGAT GCTTTGCCTC GACCCCTCCA AAAGAATAAC TGTGGAGCAG GTTGTAGGGA GCAAATACTT TGAGCATGAG GCTCGTAGGG ACGCCTCCAG CGCTGGACTC CAAGGGTGCT CGTATAGAGA AGACAGGCTC AGCCTTTCCA AAAGGAAAAA TGTCGAT
Details for EncuA.00954.a.A1.GE27819
HARVESTED ON: 11/3/2009
SEQUENCED ON: 11/10/2009
EXPECTED MW: 39kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMEPAEEG EILAHRRFTA TKMEYEKIRI IGEGTFGQVI LARKGRARYA LKKVSKEKEG LSVTTIREVQ VLRAMGHPSI VRLIEVVVEP GGDIYMVFPY FPYDLNRFIR SNKMTCSEIK HIFYQIAQGV CYIHSKGIMH RDLKSANILL DQKLNASIAD FGMARYTTKT GAYTPGMVTL WYRAPEILLG SSSYTYAVDI WSLGCILTEM YLGHMIFQGS TEMLQLEMVI HACGSINENS YPGVQDLPGF RNFRLPQSPR RIEGIIRKHD ASAVELVSKM LCLDPSKRIT VEQVVGSKYF EHEARRDASS AGLQGCSYRE DRLSLSKRKN VD
Validated NT Sequence
tattaatcga catttttcct tttggaaagg ctgagcctgt cttctctata cgagcaccct tggagtccag cgctggaggc gtccctacga gcctcatgct caaagtattt gctccctaca acctgctcca cagttattct tttggagggg tcgaggcaaa gcatcttgct gaccaactcg actgcggacg catcatgctt cctgatgatg ccttctattc tccggggaga ctggggaagc ctgaagttgc ggaatccagg gaggtcctgc actcctggat agctgttttc gttgatggat ccgcatgcat ggataaccat ctccagctgc agcatctccg tgcttccctg gaatatcatg tgccccagat acatctcggt tagtatgcac cccaatgacc agatgtccac agcatacgtg tagctggagg accccaagag tatctccggg gccctgtacc acaaagtgac cattcctggg gtgtatgctc ctgtctttgt tgtgtacctt gccatcccaa agtctgcgat gcttgcattc agcttttggt ccagaagaat gtttgcactc ttcaggtctc tgtgcatgat tcccttgctg tggatgtagc acactccctg agcaatctga tagaatatgt gcttgatctc gctgcatgtc atcttgttgc ttcttatgaa tctgttcaga tcatatggaa agtatgggaa caccatgtag atatctccgc caggctcgac aacaacctct atgagtctta ctatgcttgg atggcccatt gcccgcagaa cctggacctc cctgatcgtt gtcaccgaca atccctcctt ttccttgctt accttcttca gtgcatatct ggcccttccc tttcttgcaa gaataacctg tccgaaggtg ccttccccga tgattcttat cttctcgtac tccatcttgg ttgcagtgaa ccgtctgtgt gccaagatct ccccttcctc agcaggctcc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEPAEEGEI LAHRRFTATK MEYEKIRIIG EGTFGQVILA RKGRARYALK KVSKEKEGLS VTTIREVQVL RAMGHPSIVR LIEVVVEPGG DIYMVFPYFP YDLNRFIRSN KMTCSEIKHI FYQIAQGVCY IHSKGIMHRD LKSANILLDQ KLNASIADFG MARYTTKTGA YTPGMVTLWY RAPEILLGSS SYTYAVDIWS LGCILTEMYL GHMIFQGSTE MLQLEMVIHA CGSINENSYP GVQDLPGFRN FRLPQSPRRI EGIIRKHDAS AVELVSKMLC LDPSKRITVE QVVGSKYFEH EARRDASSAG LQGCSYREDR LSLSKRKNVD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggagcctgct gaggaagggg agatcttggc acacagacgg ttcactgcaa ccaagatgga gtacgagaag ataagaatca tcggggaagg caccttcgga caggttattc ttgcaagaaa gggaagggcc agatatgcac tgaagaaggt aagcaaggaa aaggagggat tgtcggtgac aacgatcagg gaggtccagg ttctgcgggc aatgggccat ccaagcatag taagactcat agaggttgtt gtcgagcctg gcggagatat ctacatggtg ttcccatact ttccatatga tctgaacaga ttcataagaa gcaacaagat gacatgcagc gagatcaagc acatattcta tcagattgct cagggagtgt gctacatcca cagcaaggga atcatgcaca gagacctgaa gagtgcaaac attcttctgg accaaaagct gaatgcaagc atcgcagact ttgggatggc aaggtacaca acaaagacag gagcatacac cccaggaatg gtcactttgt ggtacagggc cccggagata ctcttggggt cctccagcta cacgtatgct gtggacatct ggtcattggg gtgcatacta accgagatgt atctggggca catgatattc cagggaagca cggagatgct gcagctggag atggttatcc atgcatgcgg atccatcaac gaaaacagct atccaggagt gcaggacctc cctggattcc gcaacttcag gcttccccag tctccccgga gaatagaagg catcatcagg aagcatgatg cgtccgcagt cgagttggtc agcaagatgc tttgcctcga cccctccaaa agaataactg tggagcaggt tgtagggagc aaatactttg agcatgaggc tcgtagggac gcctccagcg ctggactcca agggtgctcg tatagagaag acaggctcag cctttccaaa aggaaaaatg tcgataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for EncuA.00954.a.A1.GE28331
HARVESTED ON: 1/5/2010
SEQUENCED ON: 1/15/2010
EXPECTED MW: 39kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMEPAEEG EILAHRRFTA TKMEYEKIRI IGEGTFGQVI LARKGRARYA LKKVSKEKEG LSVTTIREVQ VLRAMGHPSI VRLIEVVVEP GGDIYMVFPY FPYDLNRFIR SNKMTCSEIK HIFYQIAQGV CYIHSKGIMH RDLKSANILL DQKLNASIAD FGMARYTTKT GAYTPGMVTL WYRAPEILLG SSSYTYAVDI WSLGCILTEM YLGHMIFQGS TEMLQLEMVI HACGSINENS YPGVQDLPGF RNFRLPQSPR RIEGIIRKHD ASAVELVSKM LCLDPSKRIT VEQVVGSKYF EHEARRDASS AGLQGCSYRE DRLSLSKRKN VD
Validated NT Sequence
tattaatcga catttttcct tttggaaagg ctgagcctgt cttctctata cgagcaccct tggagtccag cgctggaggc gtccctacga gcctcatgct caaagtattt gctccctaca acctgctcca cagttattct tttggagggg tcgaggcaaa gcatcttgct gaccaactcg actgcggacg catcatgctt cctgatgatg ccttctattc tccggggaga ctggggaagc ctgaagttgc ggaatccagg gaggtcctgc actcctggat agctgttttc gttgatggat ccgcatgcat ggataaccat ctccagctgc agcatctccg tgcttccctg gaatatcatg tgccccagat acatctcggt tagtatgcac cccaatgacc agatgtccac agcatacgtg tagctggagg accccaagag tatctccggg gccctgtacc acaaagtgac cattcctggg gtgtatgctc ctgtctttgt tgtgtacctt gccatcccaa agtctgcgat gcttgcattc agcttttggt ccagaagaat gtttgcactc ttcaggtctc tgtgcatgat tcccttgctg tggatgtagc acactccctg agcaatctga tagaatatgt gcttgatctc gctgcatgtc atcttgttgc ttcttatgaa tctgttcaga tcatatggaa agtatgggaa caccatgtag atatctccgc caggctcgac aacaacctct atgagtctta ctatgcttgg atggcccatt gcccgcagaa cctggacctc cctgatcgtt gtcaccgaca atccctcctt ttccttgctt accttcttca gtgcatatct ggcccttccc tttcttgcaa gaataacctg tccgaaggtg ccttccccga tgattcttat cttctcgtac tccatcttgg ttgcagtgaa ccgtctgtgt gccaagatct ccccttcctc agcaggctcc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEPAEEGEI LAHRRFTATK MEYEKIRIIG EGTFGQVILA RKGRARYALK KVSKEKEGLS VTTIREVQVL RAMGHPSIVR LIEVVVEPGG DIYMVFPYFP YDLNRFIRSN KMTCSEIKHI FYQIAQGVCY IHSKGIMHRD LKSANILLDQ KLNASIADFG MARYTTKTGA YTPGMVTLWY RAPEILLGSS SYTYAVDIWS LGCILTEMYL GHMIFQGSTE MLQLEMVIHA CGSINENSYP GVQDLPGFRN FRLPQSPRRI EGIIRKHDAS AVELVSKMLC LDPSKRITVE QVVGSKYFEH EARRDASSAG LQGCSYREDR LSLSKRKNVD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggagcctgct gaggaagggg agatcttggc acacagacgg ttcactgcaa ccaagatgga gtacgagaag ataagaatca tcggggaagg caccttcgga caggttattc ttgcaagaaa gggaagggcc agatatgcac tgaagaaggt aagcaaggaa aaggagggat tgtcggtgac aacgatcagg gaggtccagg ttctgcgggc aatgggccat ccaagcatag taagactcat agaggttgtt gtcgagcctg gcggagatat ctacatggtg ttcccatact ttccatatga tctgaacaga ttcataagaa gcaacaagat gacatgcagc gagatcaagc acatattcta tcagattgct cagggagtgt gctacatcca cagcaaggga atcatgcaca gagacctgaa gagtgcaaac attcttctgg accaaaagct gaatgcaagc atcgcagact ttgggatggc aaggtacaca acaaagacag gagcatacac cccaggaatg gtcactttgt ggtacagggc cccggagata ctcttggggt cctccagcta cacgtatgct gtggacatct ggtcattggg gtgcatacta accgagatgt atctggggca catgatattc cagggaagca cggagatgct gcagctggag atggttatcc atgcatgcgg atccatcaac gaaaacagct atccaggagt gcaggacctc cctggattcc gcaacttcag gcttccccag tctccccgga gaatagaagg catcatcagg aagcatgatg cgtccgcagt cgagttggtc agcaagatgc tttgcctcga cccctccaaa agaataactg tggagcaggt tgtagggagc aaatactttg agcatgaggc tcgtagggac gcctccagcg ctggactcca agggtgctcg tatagagaag acaggctcag cctttccaaa aggaaaaatg tcgataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa