SSGCID: Target Page

EncuA.00940.a

Dehydrodolichyl diphosphate synthetase

CENTER ID:	EncuA.00940.a
ORGANISM:	Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE:	Microsporidiosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EncuA.00940.a.A1.GE30863	Full length( EncuA.00940.a )		1	270

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	MicrosporidiaDB:ECU09_1870
RefSeq:	XP_955741.1
UniProt:	Q8SQJ8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIEGLKVKAL ICVVEIIENG INSIHAMGIL LFALNILKKA SVLVSRILCA WNGKLSSEYP RDPRSRLGIF KNMNIAFICD GNRRYARKLG LEDSFIKDKG LQKIYEFIEF GCFYGIKEIS FFCFALSNFK RSPEEVNKLM GLVKQKIERP KEIGIRPKFR VYGRLDLLEE DVRKRLMDIE EESKNNTSII VNIFFAYSAE DEITRGIQFN SHVDILIRTS NTKRLSNFMI RQVAKGTSVF FAKALWPELT TAHLFLILLK HRLENKYLLG

NT Sequence

ATGATAGAAG GTCTAAAAGT GAAAGCCCTA ATATGTGTGG TGGAGATCAT TGAAAACGGA ATCAATAGCA TACATGCCAT GGGGATTCTA CTCTTTGCAT TGAATATTCT TAAAAAGGCC TCTGTCCTTG TAAGCAGAAT TCTGTGTGCG TGGAATGGGA AACTATCTTC TGAGTATCCC AGGGACCCAA GAAGCAGGCT TGGTATTTTC AAAAACATGA ACATTGCATT TATCTGCGAT GGAAACAGAA GGTATGCAAG AAAGCTTGGG CTTGAAGATA GCTTCATCAA GGACAAGGGA CTCCAGAAGA TATATGAGTT CATCGAATTT GGATGTTTCT ACGGCATTAA GGAGATCTCT TTTTTCTGCT TTGCACTGAG CAACTTCAAA AGAAGTCCGG AGGAGGTGAA TAAACTGATG GGACTTGTCA AGCAGAAAAT AGAAAGACCT AAGGAGATAG GCATCAGACC AAAGTTCCGG GTTTATGGAA GGCTGGATCT TCTCGAGGAA GATGTCAGGA AGAGACTCAT GGATATTGAG GAGGAATCTA AGAACAACAC AAGTATAATT GTGAACATAT TTTTTGCATA TTCTGCAGAG GATGAAATCA CCAGGGGAAT TCAATTCAAC AGCCATGTTG ACATACTCAT AAGGACTAGT AACACAAAAA GACTCAGCAA CTTCATGATC CGCCAAGTTG CAAAGGGGAC ATCTGTGTTC TTTGCAAAGG CACTCTGGCC TGAGCTGACA ACAGCACATC TCTTCCTGAT ACTTCTGAAG CATCGACTGG AAAATAAATA TTTATTGGGT

Details for EncuA.00940.a.A1.GE30863

HARVESTED ON:	11/16/2010
SEQUENCED ON:	11/23/2010
EXPECTED MW:	33kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMIEGLKVKA LICVVEIIEN GINSIHAMGI LLFALNILKK ASVLVSRILC AWNGKLSSEY PRDPRSRLGI FKNMNIAFIC DGNRRYARKL GLEDSFIKDK GLQKIYEFIE FGCFYGIKEI SFFCFALSNF KRSPEEVNKL MGLVKQKIER PKEIGIRPKF RVYGRLDLLE EDVRKRLMDI EEESKNNTSI IVNIFFAYSA EDEITRGIQF NSHVDILIRT SNTKRLSNFM IRQVAKGTSV FFAKALWPEL TTAHLFLILL KHRLENKYLL G

Validated NT Sequence

ttattaaccc aataaatatt tattttccag tcgatgcttc agaagtatca ggaagagatg tgctgttgtc agctcaggcc agagtgcctt tgcaaagaac acagatgtcc cctttgcaac ttggcggatc atgaagttgc tgagtctttt tgtgttacta gtccttatga gtatgtcaac atggctgttg aattgaattc ccctggtgat ttcatcctct gcagaatatg caaaaaatat gttcacaatt atacttgtgt tgttcttaga ttcctcctca atatccatga gtctcttcct gacatcttcc tcgagaagat ccagccttcc ataaacccgg aactttggtc tgatgcctat ctccttaggt ctttctattt tctgcttgac aagtcccatc agtttattca cctcctccgg acttcttttg aagttgctca gtgcaaagca gaaaaaagag atctccttaa tgccgtagaa acatccaaat tcgatgaact catatatctt ctggagtccc ttgtccttga tgaagctatc ttcaagccca agctttcttg cataccttct gtttccatcg cagataaatg caatgttcat gtttttgaaa ataccaagcc tgcttcttgg gtccctggga tactcagaag atagtttccc attccacgca cacagaattc tgcttacaag gacagaggcc tttttaagaa tattcaatgc aaagagtaga atccccatgg catgtatgct attgattccg ttttcaatga tctccaccac acatattagg gctttcactt ttagaccttc tatcatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatagaaggt ctaaaagtga aagccctaat atgtgtggtg gagatcattg aaaacggaat caatagcata catgccatgg ggattctact ctttgcattg aatattctta aaaaggcctc tgtccttgta agcagaattc tgtgtgcgtg gaatgggaaa ctatcttctg agtatcccag ggacccaaga agcaggcttg gtattttcaa aaacatgaac attgcattta tctgcgatgg aaacagaagg tatgcaagaa agcttgggct tgaagatagc ttcatcaagg acaagggact ccagaagata tatgagttca tcgaatttgg atgtttctac ggcattaagg agatctcttt tttctgcttt gcactgagca acttcaaaag aagtccggag gaggtgaata aactgatggg acttgtcaag cagaaaatag aaagacctaa ggagataggc atcagaccaa agttccgggt ttatggaagg ctggatcttc tcgaggaaga tgtcaggaag agactcatgg atattgagga ggaatctaag aacaacacaa gtataattgt gaacatattt tttgcatatt ctgcagagga tgaaatcacc aggggaattc aattcaacag ccatgttgac atactcataa ggactagtaa cacaaaaaga ctcagcaact tcatgatccg ccaagttgca aaggggacat ctgtgttctt tgcaaaggca ctctggcctg agctgacaac agcacatctc ttcctgatac ttctgaagca tcgactggaa aataaatatt tattgggtta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a