EncuA.00939.a

RIBOSOMAL PROTEIN S6 KINASE

CENTER ID: EncuA.00939.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.00939.a.A1.GE27817 Full length( EncuA.00939.a ) 1 348
EncuA.00939.a.A1.GE28330 Full length( EncuA.00939.a ) 1 348
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU10_0570
RefSeq: NP_586172.1
UniProt: Q8SR57

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPSKLPDRNA HRMIRKEDIM CVEKTGFGPF WEEFLVFIEV GERIGGLYGL RVMKKKKIVD YGEQNMMHHE YYVRSRIRHS FLINTICGFQ DYEHLFLLSE QSQNYLIKLI NSEGRFSAKV SRFYLAEILL AIQHLHSKGL SYGFLSPSNM MIGDDGHIKL KYDFLNRIDC VVGDPERNVE YASIDYKERG EISPVSDYWS MGIILYYMLV GVTPFAAPDT ALVVARIKRG RIEFPEFLDE NARDLISRLL AVSPRDRLGY AEGDAETIRR HPFFGGMNWE EVLQKKVAPP FLFNTTRKEC LSRSANLKVL YTTDYLPDQK DGYGQTFRGY GATYAPDTYR KIWRCGYI
NT Sequence
ATGCCCTCAA AATTACCGGA TAGAAACGCC CATCGGATGA TAAGAAAGGA GGATATAATG TGTGTCGAGA AAACGGGCTT CGGGCCGTTC TGGGAGGAGT TTCTTGTGTT CATAGAGGTC GGCGAAAGGA TCGGGGGTCT TTATGGCCTC AGGGTAATGA AGAAGAAAAA GATAGTGGAC TATGGAGAGC AGAACATGAT GCACCACGAA TACTATGTTC GATCGAGGAT AAGGCATTCC TTCCTTATCA ATACAATATG TGGATTCCAG GACTACGAGC ATCTATTCCT GCTCTCTGAA CAGTCGCAGA ACTACCTTAT CAAGCTGATC AACTCTGAAG GAAGATTCTC GGCAAAGGTG TCCAGGTTCT ATCTGGCAGA GATTCTGCTG GCAATCCAGC ATTTGCATTC AAAAGGGCTT TCGTATGGAT TTCTATCGCC AAGCAACATG ATGATAGGAG ACGACGGACA CATAAAGCTG AAGTACGACT TCCTAAACAG GATAGATTGT GTTGTTGGGG ATCCCGAGCG CAACGTTGAG TATGCATCGA TAGATTATAA GGAGAGGGGA GAAATCAGCC CTGTGTCTGA CTACTGGTCG ATGGGCATAA TACTATACTA CATGCTTGTC GGGGTTACTC CGTTTGCGGC ACCGGACACT GCCCTGGTCG TTGCGAGAAT TAAAAGGGGC AGGATCGAGT TTCCAGAGTT TCTCGATGAA AATGCCAGGG ACCTGATATC GAGGCTTCTG GCTGTTTCTC CTAGGGATAG GCTTGGATAT GCAGAAGGTG ATGCAGAGAC TATAAGAAGA CATCCATTTT TTGGCGGGAT GAACTGGGAG GAGGTTCTGC AGAAAAAGGT TGCACCGCCG TTTCTGTTTA ATACCACAAG AAAAGAGTGC CTTTCGAGAT CTGCAAACCT CAAGGTTCTT TATACAACAG ACTATCTTCC CGATCAAAAG GATGGATATG GGCAAACATT CCGAGGATAC GGTGCCACAT ATGCACCAGA CACCTATAGA AAGATATGGA GATGCGGCTA TATC
Details for EncuA.00939.a.A1.GE27817
HARVESTED ON: 11/3/2009
SEQUENCED ON: 1/13/2010
EXPECTED MW: 42kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPSKLPDRN AHRMIRKEDI MCVEKTGFGP FWEEFLVFIE VGERIGGLYG LRVMKKKKIV DYGEQNMMHH EYYVRSRIRH SFLINTICGF QDYEHLFLLS EQSQNYLIKL INSEGRFSAK VSRFYLAEIL LAIQHLHSKG LSYGFLSPSN MMIGDDGRIK LKYDFLNRID CVVGDPERNV EYASIDYKER GEISPVSDYW SMGIILYYML VGVTPFAAPD TALVVARIKR GRIEFPEFLD ENARDLISRL LAVSPRDRLG YAEGDAETIR RHPFFGGMNW EEVLQKKVAP PFLFNTTRKE CLSRSANLKV LYTTDYLPDQ KDGYGQTFRG YGATYAPDTY RKIWRCG
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgccct caaaattacc ggatagaaac gcccatcgga tgataagaaa ggaggatata atgtgtgtcg agaaaacggg cttcgggccg ttctgggagg agtttcttgt gttcatagag gtcggcgaaa ggatcggggg tctttatggc ctcagggtaa tgaagaagaa aaagatagtg gactatggag agcagaacat gatgcaccac gaatactatg ttcgatcgag gataaggcat tccttcctta tcaatacaat atgtggattc caggactacg agcatctatt cctgctctct gaacagtcgc agaactacct tatcaagctg atcaactctg aaggaagatt ctcggcaaag gtgtccaggt tctatctggc agagattctg ctggcaatcc agcatttgca ttcaaaaggg ctttcgtatg gatttctatc gccaagcaac atgatgatag gagacgacgg acgcataaag ctgaagtacg acttcctaaa caggatagat tgtgttgttg gggatcccga gcgcaacgtt gagtatgcat cgatagatta taaggagagg ggagaaatca gccctgtgtc tgactactgg tcgatgggca taatactata ctacatgctt gtcggggtta ctccgtttgc ggcaccggac actgccctgg tcgttgcgag aattaaaagg ggcaggatcg agtttccaga gtttctcgat gaaaatgcca gggacctgat atcgaggctt ctggctgttt ctcctaggga taggcttgga tatgcagaag gtgatgcaga gactataaga agacatccat tttttggcgg gatgaactgg gaggaggttc tgcagaaaaa ggttgcaccg ccgtttctgt ttaataccac aagaaaagag tgcctttcga gatctgcaaa cctcaaggtt ctttatacaa cagactatct tcccgatcaa aaggatggat atgggcaaac attccgagga tacggtgcca catatgcacc agacacctat agaaagatat ggagatgcgg ct
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPSKLPDRN AHRMIRKEDI MCVEKTGFGP FWEEFLVFIE VGERIGGLYG LRVMKKKKIV DYGEQNMMHH EYYVRSRIRH SFLINTICGF QDYEHLFLLS EQSQNYLIKL INSEGRFSAK VSRFYLAEIL LAIQHLHSKG LSYGFLSPSN MMIGDDGHIK LKYDFLNRID CVVGDPERNV EYASIDYKER GEISPVSDYW SMGIILYYML VGVTPFAAPD TALVVARIKR GRIEFPEFLD ENARDLISRL LAVSPRDRLG YAEGDAETIR RHPFFGGMNW EEVLQKKVAP PFLFNTTRKE CLSRSANLKV LYTTDYLPDQ KDGYGQTFRG YGATYAPDTY RKIWRCGYI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccctcaaaa ttaccggata gaaacgccca tcggatgata agaaaggagg atataatgtg tgtcgagaaa acgggcttcg ggccgttctg ggaggagttt cttgtgttca tagaggtcgg cgaaaggatc gggggtcttt atggcctcag ggtaatgaag aagaaaaaga tagtggacta tggagagcag aacatgatgc accacgaata ctatgttcga tcgaggataa ggcattcctt ccttatcaat acaatatgtg gattccagga ctacgagcat ctattcctgc tctctgaaca gtcgcagaac taccttatca agctgatcaa ctctgaagga agattctcgg caaaggtgtc caggttctat ctggcagaga ttctgctggc aatccagcat ttgcattcaa aagggctttc gtatggattt ctatcgccaa gcaacatgat gataggagac gacggacaca taaagctgaa gtacgacttc ctaaacagga tagattgtgt tgttggggat cccgagcgca acgttgagta tgcatcgata gattataagg agaggggaga aatcagccct gtgtctgact actggtcgat gggcataata ctatactaca tgcttgtcgg ggttactccg tttgcggcac cggacactgc cctggtcgtt gcgagaatta aaaggggcag gatcgagttt ccagagtttc tcgatgaaaa tgccagggac ctgatatcga ggcttctggc tgtttctcct agggataggc ttggatatgc agaaggtgat gcagagacta taagaagaca tccatttttt ggcgggatga actgggagga ggttctgcag aaaaaggttg caccgccgtt tctgtttaat accacaagaa aagagtgcct ttcgagatct gcaaacctca aggttcttta tacaacagac tatcttcccg atcaaaagga tggatatggg caaacattcc gaggatacgg tgccacatat gcaccagaca cctatagaaa gatatggaga tgcggctata tcaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for EncuA.00939.a.A1.GE28330
HARVESTED ON: 1/5/2010
SEQUENCED ON: 1/15/2010
EXPECTED MW: 42kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPSKLPDRN AHRMIRKEDI MCVEKTGFGP FWEEFLVFIE VGERIGGLYG LRVMKKKKIV DYGEQNMMHH EYYVRSRIRH SFLINTICGF QDYEHLFLLS EQSQNYLIKL INSEGRFSAK VSRFYLAEIL LAIQHLHSKG LSYGFLSPSN MMIGDDGRIK LKYDFLNRID CVVGDPERNV EYASIDYKER GEISPVSDYW SMGIILYYML VGVTPFAAPD TALVVARIKR GRIEFPEFLD ENARDLISRL LAVSPRDRLG YAEGDAETIR RHPFFGGMNW EEVLQKKVAP PFLFNTTRKE CLSRSANLKV LYTTDYLPDQ KDGYGQTFRG YGATYAPDTY RKIWRCG
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgccct caaaattacc ggatagaaac gcccatcgga tgataagaaa ggaggatata atgtgtgtcg agaaaacggg cttcgggccg ttctgggagg agtttcttgt gttcatagag gtcggcgaaa ggatcggggg tctttatggc ctcagggtaa tgaagaagaa aaagatagtg gactatggag agcagaacat gatgcaccac gaatactatg ttcgatcgag gataaggcat tccttcctta tcaatacaat atgtggattc caggactacg agcatctatt cctgctctct gaacagtcgc agaactacct tatcaagctg atcaactctg aaggaagatt ctcggcaaag gtgtccaggt tctatctggc agagattctg ctggcaatcc agcatttgca ttcaaaaggg ctttcgtatg gatttctatc gccaagcaac atgatgatag gagacgacgg acgcataaag ctgaagtacg acttcctaaa caggatagat tgtgttgttg gggatcccga gcgcaacgtt gagtatgcat cgatagatta taaggagagg ggagaaatca gccctgtgtc tgactactgg tcgatgggca taatactata ctacatgctt gtcggggtta ctccgtttgc ggcaccggac actgccctgg tcgttgcgag aattaaaagg ggcaggatcg agtttccaga gtttctcgat gaaaatgcca gggacctgat atcgaggctt ctggctgttt ctcctaggga taggcttgga tatgcagaag gtgatgcaga gactataaga agacatccat tttttggcgg gatgaactgg gaggaggttc tgcagaaaaa ggttgcaccg ccgtttctgt ttaataccac aagaaaagag tgcctttcga gatctgcaaa cctcaaggtt ctttatacaa cagactatct tcccgatcaa aaggatggat atgggcaaac attccgagga tacggtgcca catatgcacc agacacctat agaaagatat ggagatgcgg ct
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPSKLPDRN AHRMIRKEDI MCVEKTGFGP FWEEFLVFIE VGERIGGLYG LRVMKKKKIV DYGEQNMMHH EYYVRSRIRH SFLINTICGF QDYEHLFLLS EQSQNYLIKL INSEGRFSAK VSRFYLAEIL LAIQHLHSKG LSYGFLSPSN MMIGDDGHIK LKYDFLNRID CVVGDPERNV EYASIDYKER GEISPVSDYW SMGIILYYML VGVTPFAAPD TALVVARIKR GRIEFPEFLD ENARDLISRL LAVSPRDRLG YAEGDAETIR RHPFFGGMNW EEVLQKKVAP PFLFNTTRKE CLSRSANLKV LYTTDYLPDQ KDGYGQTFRG YGATYAPDTY RKIWRCGYI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccctcaaaa ttaccggata gaaacgccca tcggatgata agaaaggagg atataatgtg tgtcgagaaa acgggcttcg ggccgttctg ggaggagttt cttgtgttca tagaggtcgg cgaaaggatc gggggtcttt atggcctcag ggtaatgaag aagaaaaaga tagtggacta tggagagcag aacatgatgc accacgaata ctatgttcga tcgaggataa ggcattcctt ccttatcaat acaatatgtg gattccagga ctacgagcat ctattcctgc tctctgaaca gtcgcagaac taccttatca agctgatcaa ctctgaagga agattctcgg caaaggtgtc caggttctat ctggcagaga ttctgctggc aatccagcat ttgcattcaa aagggctttc gtatggattt ctatcgccaa gcaacatgat gataggagac gacggacaca taaagctgaa gtacgacttc ctaaacagga tagattgtgt tgttggggat cccgagcgca acgttgagta tgcatcgata gattataagg agaggggaga aatcagccct gtgtctgact actggtcgat gggcataata ctatactaca tgcttgtcgg ggttactccg tttgcggcac cggacactgc cctggtcgtt gcgagaatta aaaggggcag gatcgagttt ccagagtttc tcgatgaaaa tgccagggac ctgatatcga ggcttctggc tgtttctcct agggataggc ttggatatgc agaaggtgat gcagagacta taagaagaca tccatttttt ggcgggatga actgggagga ggttctgcag aaaaaggttg caccgccgtt tctgtttaat accacaagaa aagagtgcct ttcgagatct gcaaacctca aggttcttta tacaacagac tatcttcccg atcaaaagga tggatatggg caaacattcc gaggatacgg tgccacatat gcaccagaca cctatagaaa gatatggaga tgcggctata tcaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa