EncuA.00932.a

TYROSYL tRNA SYNTHETASE

CENTER ID: EncuA.00932.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.00932.a.A1.GE27815 Full length( EncuA.00932.a ) 1 337
EncuA.00932.a.A1.GE28329 Full length( EncuA.00932.a ) 1 337
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.00932.a.A1.PS00455 Full length( EncuA.00932.a ) 1 337
External Resources
RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU05_1120i
OrthoMCL: OG5_127567
RefSeq: NP_597455.1
UniProt: Q8SRV7
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSVEQKLYLI TRNLQEILGE EELKRIVSER ELNVYWGTAI TGKPHIAYLV PLMKIKDFVD AGCNVKILFA DIHGFLDNLK APIEKVQHRC AYYEKLIKSA LKMLCVDLDR IQFVKGSEFQ KSERYTMDLY RILSITSKHD AKKAGAEVVR QVENPMVSSL VYPSMQALDE VHLSVDAQFG GVDQRKIFTY ARKYLPLLNY EKRIHLMSPM LPGLNSDKMS SSDDLSKIDL MDSKEAIWRK IRKCFCEEGN KDNGLMMIFS HIVFPILQLK GECVRITDRD GREMAFEKYQ EFEEEFVRKS IHPGDLKSNA ARLIDEIIRP IREEMEKDLD MVREAYN
NT Sequence
ATGAGCGTCG AGCAGAAGCT GTATCTAATT ACAAGAAACC TCCAGGAGAT ACTGGGTGAG GAGGAACTGA AGAGGATTGT TTCTGAAAGA GAGCTAAATG TCTACTGGGG GACTGCAATA ACCGGAAAGC CTCATATAGC ATATCTGGTC CCCCTGATGA AGATAAAGGA CTTTGTGGAT GCTGGATGCA ACGTAAAGAT TTTATTTGCA GATATCCACG GGTTTCTGGA TAATCTCAAG GCACCCATCG AGAAAGTCCA GCATAGATGC GCATACTACG AGAAGCTAAT CAAGTCTGCG CTGAAGATGC TGTGCGTCGA CCTCGATAGA ATCCAGTTTG TCAAGGGCAG CGAGTTCCAA AAGTCTGAGA GATACACAAT GGACCTGTAT CGGATTCTAT CCATAACATC CAAGCACGAC GCAAAAAAAG CCGGAGCAGA GGTTGTCAGG CAGGTGGAAA ACCCGATGGT AAGCTCGTTG GTGTATCCGT CGATGCAGGC TCTTGACGAG GTGCATCTGT CTGTGGATGC ACAGTTCGGA GGAGTCGACC AGAGAAAGAT ATTTACATAT GCTAGGAAAT ACCTCCCGCT TCTCAACTAC GAGAAGAGAA TACACTTGAT GAGCCCAATG CTTCCGGGGC TGAACTCAGA CAAGATGAGC AGCTCCGACG ACCTTTCGAA GATTGACCTT ATGGACAGCA AGGAAGCCAT CTGGAGAAAG ATAAGAAAAT GCTTCTGCGA GGAGGGAAAC AAGGACAACG GCCTGATGAT GATTTTTTCC CACATTGTCT TCCCCATCCT TCAGCTCAAG GGAGAGTGTG TTAGAATTAC CGATAGAGAT GGAAGGGAGA TGGCCTTTGA AAAGTACCAG GAGTTTGAGG AGGAGTTTGT CAGGAAATCG ATCCATCCCG GAGACCTCAA GAGTAATGCA GCACGACTTA TTGATGAGAT TATCAGGCCC ATAAGAGAAG AGATGGAGAA GGACTTGGAC ATGGTCAGAG AGGCCTACAA T
Details for EncuA.00932.a.A1.GE27815
HARVESTED ON: 11/3/2009
SEQUENCED ON: 11/10/2009
EXPECTED MW: 41kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSVEQKLYL ITRNLQEILG EEELKRIVSE RELNVYWGTA ITGKPHIAYL VPLMKIKDFV DAGCNVKILF ADIHGFLDNL KAPIEKVQHR CAYYEKLIKS ALKMLCVDLD RIQFVKGSEF QKSERYTMDL HRILSITSKH DAKKAGAEVV RQVENPMVSS LVYPSMQALD EVHLSVDAQF GGVDQRKIFT YARKYLPLLN YEKRIHLMSP MLPGLNSDKM SSSDDLSKID LMDSKEAIWR KIRKCFCEEG NKDNGLMMIF SHIVFPILQL KGECVRITDR DGREMAFEKY QEFEEEFVRK SIHPGDLKSN AARLIDEIIR PIREEMEKDL DMVREA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagcg tcgagcagaa gctgtatcta attacaagaa acctccagga gatactgggt gaggaggaac tgaagaggat tgtttctgaa agagagctaa atgtctactg ggggactgca ataaccggaa agcctcatat agcatatctg gtccccctga tgaagataaa ggactttgtg gatgctggat gcaacgtaaa gattttattt gcagatatcc acgggtttct ggataatctc aaggcaccca tcgagaaagt ccagcataga tgcgcatact acgagaagct aatcaagtct gcgctgaaga tgctgtgcgt cgacctcgat agaatccagt ttgtcaaggg cagcgagttc caaaagtctg agagatacac aatggacctg catcggattc tatccataac atccaagcac gacgcaaaaa aagccggagc agaggttgtc aggcaggtgg aaaacccgat ggtaagctcg ttggtgtatc cgtcgatgca ggctcttgac gaggtgcatc tgtctgtgga tgcacagttc ggaggagtcg accagagaaa gatatttaca tatgctagga aatacctccc gcttctcaac tacgagaaga gaatacactt gatgagccca atgcttccgg ggctgaactc agacaagatg agcagctccg acgacctttc gaagattgac cttatggaca gcaaggaagc catctggaga aagataagaa aatgcttctg cgaggaggga aacaaggaca acggcctgat gatgattttt tcccacattg tcttccccat ccttcagctc aagggagagt gtgttagaat taccgataga gatggaaggg agatggcctt tgaaaagtac caggagtttg aggaggagtt tgtcaggaaa tcgatccatc ccggagacct caagagtaat gcagcacgac ttattgatga gattatcagg cccataagag aagagatgga gaaggacttg gacatggtca gagaggcct
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSVEQKLYL ITRNLQEILG EEELKRIVSE RELNVYWGTA ITGKPHIAYL VPLMKIKDFV DAGCNVKILF ADIHGFLDNL KAPIEKVQHR CAYYEKLIKS ALKMLCVDLD RIQFVKGSEF QKSERYTMDL YRILSITSKH DAKKAGAEVV RQVENPMVSS LVYPSMQALD EVHLSVDAQF GGVDQRKIFT YARKYLPLLN YEKRIHLMSP MLPGLNSDKM SSSDDLSKID LMDSKEAIWR KIRKCFCEEG NKDNGLMMIF SHIVFPILQL KGECVRITDR DGREMAFEKY QEFEEEFVRK SIHPGDLKSN AARLIDEIIR PIREEMEKDL DMVREAYN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgtcgag cagaagctgt atctaattac aagaaacctc caggagatac tgggtgagga ggaactgaag aggattgttt ctgaaagaga gctaaatgtc tactggggga ctgcaataac cggaaagcct catatagcat atctggtccc cctgatgaag ataaaggact ttgtggatgc tggatgcaac gtaaagattt tatttgcaga tatccacggg tttctggata atctcaaggc acccatcgag aaagtccagc atagatgcgc atactacgag aagctaatca agtctgcgct gaagatgctg tgcgtcgacc tcgatagaat ccagtttgtc aagggcagcg agttccaaaa gtctgagaga tacacaatgg acctgtatcg gattctatcc ataacatcca agcacgacgc aaaaaaagcc ggagcagagg ttgtcaggca ggtggaaaac ccgatggtaa gctcgttggt gtatccgtcg atgcaggctc ttgacgaggt gcatctgtct gtggatgcac agttcggagg agtcgaccag agaaagatat ttacatatgc taggaaatac ctcccgcttc tcaactacga gaagagaata cacttgatga gcccaatgct tccggggctg aactcagaca agatgagcag ctccgacgac ctttcgaaga ttgaccttat ggacagcaag gaagccatct ggagaaagat aagaaaatgc ttctgcgagg agggaaacaa ggacaacggc ctgatgatga ttttttccca cattgtcttc cccatccttc agctcaaggg agagtgtgtt agaattaccg atagagatgg aagggagatg gcctttgaaa agtaccagga gtttgaggag gagtttgtca ggaaatcgat ccatcccgga gacctcaaga gtaatgcagc acgacttatt gatgagatta tcaggcccat aagagaagag atggagaagg acttggacat ggtcagagag gcctacaata aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for EncuA.00932.a.A1.GE28329
HARVESTED ON: 1/5/2010
SEQUENCED ON: 1/15/2010
EXPECTED MW: 41kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSVEQKLYL ITRNLQEILG EEELKRIVSE RELNVYWGTA ITGKPHIAYL VPLMKIKDFV DAGCNVKILF ADIHGFLDNL KAPIEKVQHR CAYYEKLIKS ALKMLCVDLD RIQFVKGSEF QKSERYTMDL HRILSITSKH DAKKAGAEVV RQVENPMVSS LVYPSMQALD EVHLSVDAQF GGVDQRKIFT YARKYLPLLN YEKRIHLMSP MLPGLNSDKM SSSDDLSKID LMDSKEAIWR KIRKCFCEEG NKDNGLMMIF SHIVFPILQL KGECVRITDR DGREMAFEKY QEFEEEFVRK SIHPGDLKSN AARLIDEIIR PIREEMEKDL DMVREA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagcg tcgagcagaa gctgtatcta attacaagaa acctccagga gatactgggt gaggaggaac tgaagaggat tgtttctgaa agagagctaa atgtctactg ggggactgca ataaccggaa agcctcatat agcatatctg gtccccctga tgaagataaa ggactttgtg gatgctggat gcaacgtaaa gattttattt gcagatatcc acgggtttct ggataatctc aaggcaccca tcgagaaagt ccagcataga tgcgcatact acgagaagct aatcaagtct gcgctgaaga tgctgtgcgt cgacctcgat agaatccagt ttgtcaaggg cagcgagttc caaaagtctg agagatacac aatggacctg catcggattc tatccataac atccaagcac gacgcaaaaa aagccggagc agaggttgtc aggcaggtgg aaaacccgat ggtaagctcg ttggtgtatc cgtcgatgca ggctcttgac gaggtgcatc tgtctgtgga tgcacagttc ggaggagtcg accagagaaa gatatttaca tatgctagga aatacctccc gcttctcaac tacgagaaga gaatacactt gatgagccca atgcttccgg ggctgaactc agacaagatg agcagctccg acgacctttc gaagattgac cttatggaca gcaaggaagc catctggaga aagataagaa aatgcttctg cgaggaggga aacaaggaca acggcctgat gatgattttt tcccacattg tcttccccat ccttcagctc aagggagagt gtgttagaat taccgataga gatggaaggg agatggcctt tgaaaagtac caggagtttg aggaggagtt tgtcaggaaa tcgatccatc ccggagacct caagagtaat gcagcacgac ttattgatga gattatcagg cccataagag aagagatgga gaaggacttg gacatggtca gagaggcct
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSVEQKLYL ITRNLQEILG EEELKRIVSE RELNVYWGTA ITGKPHIAYL VPLMKIKDFV DAGCNVKILF ADIHGFLDNL KAPIEKVQHR CAYYEKLIKS ALKMLCVDLD RIQFVKGSEF QKSERYTMDL YRILSITSKH DAKKAGAEVV RQVENPMVSS LVYPSMQALD EVHLSVDAQF GGVDQRKIFT YARKYLPLLN YEKRIHLMSP MLPGLNSDKM SSSDDLSKID LMDSKEAIWR KIRKCFCEEG NKDNGLMMIF SHIVFPILQL KGECVRITDR DGREMAFEKY QEFEEEFVRK SIHPGDLKSN AARLIDEIIR PIREEMEKDL DMVREAYN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgtcgag cagaagctgt atctaattac aagaaacctc caggagatac tgggtgagga ggaactgaag aggattgttt ctgaaagaga gctaaatgtc tactggggga ctgcaataac cggaaagcct catatagcat atctggtccc cctgatgaag ataaaggact ttgtggatgc tggatgcaac gtaaagattt tatttgcaga tatccacggg tttctggata atctcaaggc acccatcgag aaagtccagc atagatgcgc atactacgag aagctaatca agtctgcgct gaagatgctg tgcgtcgacc tcgatagaat ccagtttgtc aagggcagcg agttccaaaa gtctgagaga tacacaatgg acctgtatcg gattctatcc ataacatcca agcacgacgc aaaaaaagcc ggagcagagg ttgtcaggca ggtggaaaac ccgatggtaa gctcgttggt gtatccgtcg atgcaggctc ttgacgaggt gcatctgtct gtggatgcac agttcggagg agtcgaccag agaaagatat ttacatatgc taggaaatac ctcccgcttc tcaactacga gaagagaata cacttgatga gcccaatgct tccggggctg aactcagaca agatgagcag ctccgacgac ctttcgaaga ttgaccttat ggacagcaag gaagccatct ggagaaagat aagaaaatgc ttctgcgagg agggaaacaa ggacaacggc ctgatgatga ttttttccca cattgtcttc cccatccttc agctcaaggg agagtgtgtt agaattaccg atagagatgg aagggagatg gcctttgaaa agtaccagga gtttgaggag gagtttgtca ggaaatcgat ccatcccgga gacctcaaga gtaatgcagc acgacttatt gatgagatta tcaggcccat aagagaagag atggagaagg acttggacat ggtcagagag gcctacaata aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for EncuA.00932.a.A1.PS00455
PURIFICATION DATe: 1/7/2010
CONCENTRATION: 26.4mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSVEQKLYL ITRNLQEILG EEELKRIVSE RELNVYWGTA ITGKPHIAYL VPLMKIKDFV DAGCNVKILF ADIHGFLDNL KAPIEKVQHR CAYYEKLIKS ALKMLCVDLD RIQFVKGSEF QKSERYTMDL HRILSITSKH DAKKAGAEVV RQVENPMVSS LVYPSMQALD EVHLSVDAQF GGVDQRKIFT YARKYLPLLN YEKRIHLMSP MLPGLNSDKM SSSDDLSKID LMDSKEAIWR KIRKCFCEEG NKDNGLMMIF SHIVFPILQL KGECVRITDR DGREMAFEKY QEFEEEFVRK SIHPGDLKSN AARLIDEIIR PIREEMEKDL DMVREA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagcg tcgagcagaa gctgtatcta attacaagaa acctccagga gatactgggt gaggaggaac tgaagaggat tgtttctgaa agagagctaa atgtctactg ggggactgca ataaccggaa agcctcatat agcatatctg gtccccctga tgaagataaa ggactttgtg gatgctggat gcaacgtaaa gattttattt gcagatatcc acgggtttct ggataatctc aaggcaccca tcgagaaagt ccagcataga tgcgcatact acgagaagct aatcaagtct gcgctgaaga tgctgtgcgt cgacctcgat agaatccagt ttgtcaaggg cagcgagttc caaaagtctg agagatacac aatggacctg catcggattc tatccataac atccaagcac gacgcaaaaa aagccggagc agaggttgtc aggcaggtgg aaaacccgat ggtaagctcg ttggtgtatc cgtcgatgca ggctcttgac gaggtgcatc tgtctgtgga tgcacagttc ggaggagtcg accagagaaa gatatttaca tatgctagga aatacctccc gcttctcaac tacgagaaga gaatacactt gatgagccca atgcttccgg ggctgaactc agacaagatg agcagctccg acgacctttc gaagattgac cttatggaca gcaaggaagc catctggaga aagataagaa aatgcttctg cgaggaggga aacaaggaca acggcctgat gatgattttt tcccacattg tcttccccat ccttcagctc aagggagagt gtgttagaat taccgataga gatggaaggg agatggcctt tgaaaagtac caggagtttg aggaggagtt tgtcaggaaa tcgatccatc ccggagacct caagagtaat gcagcacgac ttattgatga gattatcagg cccataagag aagagatgga gaaggacttg gacatggtca gagaggcct
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSVEQKLYL ITRNLQEILG EEELKRIVSE RELNVYWGTA ITGKPHIAYL VPLMKIKDFV DAGCNVKILF ADIHGFLDNL KAPIEKVQHR CAYYEKLIKS ALKMLCVDLD RIQFVKGSEF QKSERYTMDL YRILSITSKH DAKKAGAEVV RQVENPMVSS LVYPSMQALD EVHLSVDAQF GGVDQRKIFT YARKYLPLLN YEKRIHLMSP MLPGLNSDKM SSSDDLSKID LMDSKEAIWR KIRKCFCEEG NKDNGLMMIF SHIVFPILQL KGECVRITDR DGREMAFEKY QEFEEEFVRK SIHPGDLKSN AARLIDEIIR PIREEMEKDL DMVREAYN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCGTCGAG CAGAAGCTGT ATCTAATTAC AAGAAACCTC CAGGAGATAC TGGGTGAGGA GGAACTGAAG AGGATTGTTT CTGAAAGAGA GCTAAATGTC TACTGGGGGA CTGCAATAAC CGGAAAGCCT CATATAGCAT ATCTGGTCCC CCTGATGAAG ATAAAGGACT TTGTGGATGC TGGATGCAAC GTAAAGATTT TATTTGCAGA TATCCACGGG TTTCTGGATA ATCTCAAGGC ACCCATCGAG AAAGTCCAGC ATAGATGCGC ATACTACGAG AAGCTAATCA AGTCTGCGCT GAAGATGCTG TGCGTCGACC TCGATAGAAT CCAGTTTGTC AAGGGCAGCG AGTTCCAAAA GTCTGAGAGA TACACAATGG ACCTGTATCG GATTCTATCC ATAACATCCA AGCACGACGC AAAAAAAGCC GGAGCAGAGG TTGTCAGGCA GGTGGAAAAC CCGATGGTAA GCTCGTTGGT GTATCCGTCG ATGCAGGCTC TTGACGAGGT GCATCTGTCT GTGGATGCAC AGTTCGGAGG AGTCGACCAG AGAAAGATAT TTACATATGC TAGGAAATAC CTCCCGCTTC TCAACTACGA GAAGAGAATA CACTTGATGA GCCCAATGCT TCCGGGGCTG AACTCAGACA AGATGAGCAG CTCCGACGAC CTTTCGAAGA TTGACCTTAT GGACAGCAAG GAAGCCATCT GGAGAAAGAT AAGAAAATGC TTCTGCGAGG AGGGAAACAA GGACAACGGC CTGATGATGA TTTTTTCCCA CATTGTCTTC CCCATCCTTC AGCTCAAGGG AGAGTGTGTT AGAATTACCG ATAGAGATGG AAGGGAGATG GCCTTTGAAA AGTACCAGGA GTTTGAGGAG GAGTTTGTCA GGAAATCGAT CCATCCCGGA GACCTCAAGA GTAATGCAGC ACGACTTATT GATGAGATTA TCAGGCCCAT AAGAGAAGAG ATGGAGAAGG ACTTGGACAT GGTCAGAGAG GCCTACAATA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A