EncuA.00884.a

Similarity with M phase inducer phosphatase

CENTER ID:	EncuA.00884.a
ORGANISM:	Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE:	Microsporidiosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/IIIB

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EncuA.00884.a.A1.GE27814	Full length( EncuA.00884.a )		1	261

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EncuA.00884.a.A1.PW29191	Full length( EncuA.00884.a )		1	261

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	MicrosporidiaDB:ECU04_0710
OrthoMCL:	OG5_127845
RefSeq:	NP_584754.1
UniProt:	Q8SVU9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTNSSAFASS DNEQCPAAVD QMYIKPFYEI PWSSSENIEF EDPGHDPFVD NSDAIKMNHL RGSGKQGKCR EKAVWTSLQG MRGQSRAGPA PAANRHLIDF WKDFSNGEVF SLPTLGPGKS DSIQRISVDV AKQVVEGMYN IDYLVIDCRF PYEYRGGHII NAVNISSTKK LGLLFRRPRA LIFHCEFSSI RAPRLAQHLR NMDRMKNPYP LLTIPEVYVM EGGYRKFYSR YPDLCDPKGY VTMDNLDYRD LYKRDKLRSN K

NT Sequence

ATGACAAATA GCTCAGCATT TGCTTCAAGT GACAACGAAC AGTGCCCTGC AGCCGTAGAT CAGATGTACA TCAAGCCTTT CTACGAAATC CCCTGGTCGT CCAGTGAGAA TATTGAGTTC GAGGATCCTG GACACGATCC TTTTGTAGAT AATAGCGATG CGATTAAGAT GAATCATTTA AGAGGATCTG GAAAGCAAGG GAAGTGTAGA GAGAAGGCTG TGTGGACATC ACTGCAGGGA ATGAGGGGGC AAAGCAGGGC CGGCCCTGCT CCCGCTGCAA ATAGACATCT TATAGACTTC TGGAAGGACT TTAGCAACGG CGAAGTGTTT TCTCTCCCCA CCCTTGGACC TGGAAAAAGT GATAGCATCC AGAGGATTTC TGTTGATGTT GCAAAGCAGG TTGTTGAGGG GATGTATAAC ATCGACTACC TGGTCATAGA CTGCAGGTTC CCATATGAGT ATCGAGGAGG ACATATCATA AATGCAGTGA ACATCAGCTC CACCAAGAAG CTGGGCCTCC TCTTCCGAAG ACCAAGGGCT TTGATCTTCC ACTGCGAGTT CTCATCCATA AGGGCTCCGA GACTTGCCCA GCATCTCAGG AACATGGACA GGATGAAGAA TCCATATCCG CTGCTGACCA TCCCTGAAGT GTATGTGATG GAGGGGGGGT ACAGGAAGTT TTACAGCAGG TATCCGGACC TGTGCGATCC AAAGGGATAT GTGACAATGG ACAACCTTGA TTATAGAGAC CTGTATAAAA GAGACAAGCT TAGATCGAAT AAA

Details for EncuA.00884.a.A1.GE27814

HARVESTED ON:	11/3/2009
SEQUENCED ON:	11/10/2009
EXPECTED MW:	32kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMTNSSAFAS SDNEQRPAAV DQMYIKPFYE IPWSSSENIE FEDPGHDPFV DNSDAIKMNH LRGSGKQGKC REKAVWTSLQ GMRGQSRAGP APAANRHLID FWKDFSNGEV FSLPTLGPGK SDSIQRISVD VAKQVVEGMY NIDYLVIDCR FPYEYRGGHI INAVNISSTK KLGLLFRRPR ALIFHCEFSS IRAPRLAQHL RNMDRMKNPY PLLTIPEVYV MEGGYRKFYS RYPDLCDPKG YVTMDNLDYR DLYKRDKLRS

Validated NT Sequence

tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgacaaa tagctcagca tttgcttcaa gtgacaacga acagcgccct gcagccgtag atcagatgta catcaagcct ttctacgaaa tcccctggtc gtccagtgag aatattgagt tcgaggatcc tggacacgat ccttttgtag ataatagcga tgcgattaag atgaatcatt taagaggatc tggaaagcaa gggaagtgta gagagaaggc tgtgtggaca tcactgcagg gaatgagggg gcaaagcagg gccggccctg ctcccgctgc aaatagacat cttatagact tctggaagga ctttagcaac ggcgaagtgt tttctctccc cacccttgga cctggaaaaa gtgatagcat ccagaggatt tctgttgatg ttgcaaagca ggttgttgag gggatgtata acatcgacta cctggtcata gactgcaggt tcccatatga gtatcgagga ggacatatca taaatgcagt gaacatcagc tccaccaaga agctgggcct cctcttccga agaccaaggg ctttgatctt ccactgcgag ttctcatcca taagggctcc gagacttgcc cagcatctca ggaacatgga caggatgaag aatccatatc cgctgctgac catccctgaa gtgtatgtga tggagggggg gtacaggaag ttttacagca ggtatccgga cctgtgcgat ccaaagggat atgtgacaat ggacaacctt gattatagag acctgtataa aagagacaag cttagatcga

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTNSSAFAS SDNEQCPAAV DQMYIKPFYE IPWSSSENIE FEDPGHDPFV DNSDAIKMNH LRGSGKQGKC REKAVWTSLQ GMRGQSRAGP APAANRHLID FWKDFSNGEV FSLPTLGPGK SDSIQRISVD VAKQVVEGMY NIDYLVIDCR FPYEYRGGHI INAVNISSTK KLGLLFRRPR ALIFHCEFSS IRAPRLAQHL RNMDRMKNPY PLLTIPEVYV MEGGYRKFYS RYPDLCDPKG YVTMDNLDYR DLYKRDKLRS NK

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacaaatagc tcagcatttg cttcaagtga caacgaacag tgccctgcag ccgtagatca gatgtacatc aagcctttct acgaaatccc ctggtcgtcc agtgagaata ttgagttcga ggatcctgga cacgatcctt ttgtagataa tagcgatgcg attaagatga atcatttaag aggatctgga aagcaaggga agtgtagaga gaaggctgtg tggacatcac tgcagggaat gagggggcaa agcagggccg gccctgctcc cgctgcaaat agacatctta tagacttctg gaaggacttt agcaacggcg aagtgttttc tctccccacc cttggacctg gaaaaagtga tagcatccag aggatttctg ttgatgttgc aaagcaggtt gttgagggga tgtataacat cgactacctg gtcatagact gcaggttccc atatgagtat cgaggaggac atatcataaa tgcagtgaac atcagctcca ccaagaagct gggcctcctc ttccgaagac caagggcttt gatcttccac tgcgagttct catccataag ggctccgaga cttgcccagc atctcaggaa catggacagg atgaagaatc catatccgct gctgaccatc cctgaagtgt atgtgatgga gggggggtac aggaagtttt acagcaggta tccggacctg tgcgatccaa agggatatgt gacaatggac aaccttgatt atagagacct gtataaaaga gacaagctta gatcgaataa aaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa

Details for EncuA.00884.a.A1.PW29191

PURIFICATION DATe:	3/22/2010
CONCENTRATION:	1.37mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	10
VIAL VOLUME:	100µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	99

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACAAATAGC TCAGCATTTG CTTCAAGTGA CAACGAACAG TGCCCTGCAG CCGTAGATCA GATGTACATC AAGCCTTTCT ACGAAATCCC CTGGTCGTCC AGTGAGAATA TTGAGTTCGA GGATCCTGGA CACGATCCTT TTGTAGATAA TAGCGATGCG ATTAAGATGA ATCATTTAAG AGGATCTGGA AAGCAAGGGA AGTGTAGAGA GAAGGCTGTG TGGACATCAC TGCAGGGAAT GAGGGGGCAA AGCAGGGCCG GCCCTGCTCC CGCTGCAAAT AGACATCTTA TAGACTTCTG GAAGGACTTT AGCAACGGCG AAGTGTTTTC TCTCCCCACC CTTGGACCTG GAAAAAGTGA TAGCATCCAG AGGATTTCTG TTGATGTTGC AAAGCAGGTT GTTGAGGGGA TGTATAACAT CGACTACCTG GTCATAGACT GCAGGTTCCC ATATGAGTAT CGAGGAGGAC ATATCATAAA TGCAGTGAAC ATCAGCTCCA CCAAGAAGCT GGGCCTCCTC TTCCGAAGAC CAAGGGCTTT GATCTTCCAC TGCGAGTTCT CATCCATAAG GGCTCCGAGA CTTGCCCAGC ATCTCAGGAA CATGGACAGG ATGAAGAATC CATATCCGCT GCTGACCATC CCTGAAGTGT ATGTGATGGA GGGGGGGTAC AGGAAGTTTT ACAGCAGGTA TCCGGACCTG TGCGATCCAA AGGGATATGT GACAATGGAC AACCTTGATT ATAGAGACCT GTATAAAAGA GACAAGCTTA GATCGAATAA AAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA