SSGCID: Target Page

EncuA.00881.a

Similarity to SER/THR CYCLIN-DEPENDENT PROTEIN KINASE KIN28

CENTER ID:	EncuA.00881.a
ORGANISM:	Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE:	Microsporidiosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EncuA.00881.a.A1.GE27240	Full length( EncuA.00881.a )		1	308

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	MicrosporidiaDB:ECU02_1450
RefSeq:	NP_584670.1
UniProt:	Q8SW92

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKTYIRERRL GEGTYAVIYL GYRALPQDKP LVSSGTRIED VPVAIKKIKP TKYTQGHEIS AIREIKSLKR IDSKYVVRLI DTFVYDKCVH IVLEYVETNL ENVIRNSDKI IMPGDIKAWI LMVLRGVYEC HRLFIIHRDI KPNNILITSE GMVKLADFGL TRGIGNRMTP QAVTRWYRAP ELLMGSRDYG SPVDMWSVGC VFAELFLRVP LFAGDTDIQQ LDMIFRALGT PVEREWPGVS TLPEFLDFQQ YPKASLNGLF SAASSDALDL LEKLLTLNPC NRISCDDAIK HPYFKSSPPP TPIGKLPV

NT Sequence

ATGAAGACAT ATATCAGGGA AAGGAGATTG GGAGAGGGGA CGTACGCCGT GATATACCTA GGATACAGGG CCCTTCCTCA GGATAAACCT CTTGTAAGTT CGGGCACGAG AATAGAAGAT GTTCCTGTGG CAATAAAGAA GATAAAGCCG ACCAAATACA CACAAGGGCA TGAGATAAGT GCCATAAGGG AAATCAAATC TCTGAAGAGG ATAGATAGTA AGTATGTTGT CAGGCTGATA GATACCTTTG TATATGACAA GTGTGTCCAT ATAGTGCTGG AGTATGTCGA GACTAATCTT GAGAATGTGA TACGGAATTC AGATAAAATC ATCATGCCTG GAGACATCAA GGCATGGATA TTGATGGTGC TAAGGGGAGT TTATGAGTGC CACAGGCTAT TTATAATCCA CCGGGACATC AAGCCAAACA ATATACTTAT TACGTCTGAA GGTATGGTTA AGCTGGCTGA CTTTGGACTT ACCAGGGGGA TAGGAAACCG GATGACACCG CAGGCCGTAA CTAGATGGTA TAGAGCGCCA GAGCTTCTCA TGGGGTCTCG AGACTATGGA AGTCCCGTTG ATATGTGGTC TGTCGGATGT GTGTTTGCAG AACTGTTTTT AAGAGTTCCC CTGTTTGCAG GAGATACAGA CATCCAGCAG CTGGATATGA TCTTTAGGGC CCTTGGAACG CCTGTGGAAA GGGAGTGGCC GGGAGTTTCC ACTTTACCCG AGTTTCTTGA TTTTCAACAA TATCCTAAGG CATCTTTGAA TGGCCTATTT TCTGCAGCAT CTTCTGATGC ATTGGACCTG CTGGAAAAGC TCCTTACTTT GAATCCGTGC AATAGAATCA GCTGCGATGA TGCTATAAAG CATCCCTATT TCAAGTCGTC ACCTCCGCCC ACACCCATAG GAAAACTTCC TGTG

Details for EncuA.00881.a.A1.GE27240

HARVESTED ON:	7/31/2009
SEQUENCED ON:	3/21/2012
EXPECTED MW:	37kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	76

Validated AA Sequence

MKTYIRERRL GEGTYAVIYL GYRALPQDKP LVSSGTRIED VPVAIKKIKP TKYTQGHEIS AIREIKSLKR IDSKYVVRLI DTFVYDKCVH IVLEYVEANL ENVIRNSDKI IMPGDIKAWI LMVLRGVYEC HRLFIIHRDI KPNNILITSE GMVKLADFGL TRGIGNRMTP QAVTRWYRAP ELLMGSRDYG SPVDMWSVGC VFAELFLRVP LFAGDTDIQQ LDMIFXALGT PVEREWPGVS TLPEFLDFXQ Y

Validated NT Sequence

gggtaccctg gaagctcaga cccagggtcc tggttcgatg aagacatata tcagggaaag gagattggga gaggggacgt acgccgtgat atacctagga tacagggccc ttcctcagga taaacctctt gtaagttcgg gcacgagaat agaagatgtt cctgtggcaa taaagaagat aaagccgacc aaatacacac aagggcatga gataagtgcc ataagggaaa tcaaatctct gaagaggata gatagtaagt atgttgtcag gctgatagat acctttgtat atgacaagtg tgtccatata gtgctggagt atgtcgaggc taatcttgag aatgtgatac ggaattcaga taaaatcatc atgcctggag acatcaaggc atggatattg atggtgctaa ggggagttta tgagtgccac aggctattta taatccaccg ggacatcaag ccaaacaata tacttattac gtctgaaggt atggttaagc tggctgactt tggacttacc agggggatag gaaaccggat gacaccgcag gccgtaacta gatggtatag agcgccagag cttctcatgg ggtctcgaga ctatggaagt cccgttgata tgtggtctgt cggatgtgtg tttgcagaac tgtttttaag agttcccctg tttgcaggag atacagacat ccagcagctg gatatgatct ttanggccct tggaacgcct gtggaaaggg agtggccggg agtttccact ttacccgagt ttcttgattt tcnacaatat cc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMKTYIRERR LGEGTYAVIY LGYRALPQDK PLVSSGTRIE DVPVAIKKIK PTKYTQGHEI SAIREIKSLK RIDSKYVVRL IDTFVYDKCV HIVLEYVETN LENVIRNSDK IIMPGDIKAW ILMVLRGVYE CHRLFIIHRD IKPNNILITS EGMVKLADFG LTRGIGNRMT PQAVTRWYRA PELLMGSRDY GSPVDMWSVG CVFAELFLRV PLFAGDTDIQ QLDMIFRALG TPVEREWPGV STLPEFLDFQ QYPKASLNGL FSAASSDALD LLEKLLTLNP CNRISCDDAI KHPYFKSSPP PTPIGKLPV

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagacatat atcagggaaa ggagattggg agaggggacg tacgccgtga tatacctagg atacagggcc cttcctcagg ataaacctct tgtaagttcg ggcacgagaa tagaagatgt tcctgtggca ataaagaaga taaagccgac caaatacaca caagggcatg agataagtgc cataagggaa atcaaatctc tgaagaggat agatagtaag tatgttgtca ggctgataga tacctttgta tatgacaagt gtgtccatat agtgctggag tatgtcgaga ctaatcttga gaatgtgata cggaattcag ataaaatcat catgcctgga gacatcaagg catggatatt gatggtgcta aggggagttt atgagtgcca caggctattt ataatccacc gggacatcaa gccaaacaat atacttatta cgtctgaagg tatggttaag ctggctgact ttggacttac cagggggata ggaaaccgga tgacaccgca ggccgtaact agatggtata gagcgccaga gcttctcatg gggtctcgag actatggaag tcccgttgat atgtggtctg tcggatgtgt gtttgcagaa ctgtttttaa gagttcccct gtttgcagga gatacagaca tccagcagct ggatatgatc tttagggccc ttggaacgcc tgtggaaagg gagtggccgg gagtttccac tttacccgag tttcttgatt ttcaacaata tcctaaggca tctttgaatg gcctattttc tgcagcatct tctgatgcat tggacctgct ggaaaagctc cttactttga atccgtgcaa tagaatcagc tgcgatgatg ctataaagca tccctatttc aagtcgtcac ctccgcccac acccatagga aaacttcctg tgaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa